Genome re-assignment of Arachis trinitensis (sect. Arachis, Leguminosae) and its implications for the genetic origin of cultivated peanut

Autores
Robledo Dobladez, Germán Ariel; Lavia, Graciela Inés; Seijo, José Guillermo
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The karyotype structure of Arachis trinitensis was studied by conventional Feulgen staining, CMA/DAPI banding and rDNA loci detection by fluorescence in situ hybridization (FISH) in order to establish its genome status and test the hypothesis that this species is a genome donor of cultivated peanut. Conventional staining revealed that the karyotype lacked the small "A chromosomes" characteristic of the A genome. In agreement with this, chromosomal banding showed that none of the chromosomes had the large centromeric bands expected for A chromosomes. FISH revealed one pair each of 5S and 45S rDNA loci, located in different medium-sized metacentric chromosomes. Collectively, these results suggest that A. trinitensis should be removed from the A genome and be considered as a B or non-A genome species. The pattern of heterochromatic bands and rDNA loci of A. trinitensis differ markedly from any of the complements of A. hypogaea, suggesting that the former species is unlikely to be one of the wild diploid progenitors of the latter. © 2010, Sociedade Brasileira de Genética.
Fil: Robledo Dobladez, Germán Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina
Fil: Lavia, Graciela Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Seijo, José Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina
Materia
CMA/DAPI BANDS
GENOME DONORS
PEANUT GENETIC ORIGIN
RDNA LOCI
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/66061
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/66061
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Genome re-assignment of Arachis trinitensis (sect. Arachis, Leguminosae) and its implications for the genetic origin of cultivated peanutRobledo Dobladez, Germán ArielLavia, Graciela InésSeijo, José GuillermoCMA/DAPI BANDSGENOME DONORSPEANUT GENETIC ORIGINRDNA LOCIhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The karyotype structure of Arachis trinitensis was studied by conventional Feulgen staining, CMA/DAPI banding and rDNA loci detection by fluorescence in situ hybridization (FISH) in order to establish its genome status and test the hypothesis that this species is a genome donor of cultivated peanut. Conventional staining revealed that the karyotype lacked the small "A chromosomes" characteristic of the A genome. In agreement with this, chromosomal banding showed that none of the chromosomes had the large centromeric bands expected for A chromosomes. FISH revealed one pair each of 5S and 45S rDNA loci, located in different medium-sized metacentric chromosomes. Collectively, these results suggest that A. trinitensis should be removed from the A genome and be considered as a B or non-A genome species. The pattern of heterochromatic bands and rDNA loci of A. trinitensis differ markedly from any of the complements of A. hypogaea, suggesting that the former species is unlikely to be one of the wild diploid progenitors of the latter. © 2010, Sociedade Brasileira de Genética.Fil: Robledo Dobladez, Germán Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; ArgentinaFil: Lavia, Graciela Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Seijo, José Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; ArgentinaSociedade Brasileira de Genética2010-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/66061Robledo Dobladez, Germán Ariel; Lavia, Graciela Inés; Seijo, José Guillermo; Genome re-assignment of Arachis trinitensis (sect. Arachis, Leguminosae) and its implications for the genetic origin of cultivated peanut; Sociedade Brasileira de Genética; Genetics and Molecular Biology; 33; 4; 4-2010; 714-7181415-47571678-4685CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1590/S1415-47572010005000079info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/49dychinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:47Zoai:ri.conicet.gov.ar:11336/66061instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:47.286CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genome re-assignment of Arachis trinitensis (sect. Arachis, Leguminosae) and its implications for the genetic origin of cultivated peanut
title Genome re-assignment of Arachis trinitensis (sect. Arachis, Leguminosae) and its implications for the genetic origin of cultivated peanut
spellingShingle Genome re-assignment of Arachis trinitensis (sect. Arachis, Leguminosae) and its implications for the genetic origin of cultivated peanut
Robledo Dobladez, Germán Ariel
CMA/DAPI BANDS
GENOME DONORS
PEANUT GENETIC ORIGIN
RDNA LOCI
title_short Genome re-assignment of Arachis trinitensis (sect. Arachis, Leguminosae) and its implications for the genetic origin of cultivated peanut
title_full Genome re-assignment of Arachis trinitensis (sect. Arachis, Leguminosae) and its implications for the genetic origin of cultivated peanut
title_fullStr Genome re-assignment of Arachis trinitensis (sect. Arachis, Leguminosae) and its implications for the genetic origin of cultivated peanut
title_full_unstemmed Genome re-assignment of Arachis trinitensis (sect. Arachis, Leguminosae) and its implications for the genetic origin of cultivated peanut
title_sort Genome re-assignment of Arachis trinitensis (sect. Arachis, Leguminosae) and its implications for the genetic origin of cultivated peanut
dc.creator.none.fl_str_mv Robledo Dobladez, Germán Ariel
Lavia, Graciela Inés
Seijo, José Guillermo
author Robledo Dobladez, Germán Ariel
author_facet Robledo Dobladez, Germán Ariel
Lavia, Graciela Inés
Seijo, José Guillermo
author_role author
author2 Lavia, Graciela Inés
Seijo, José Guillermo
author2_role author
author
dc.subject.none.fl_str_mv CMA/DAPI BANDS
GENOME DONORS
PEANUT GENETIC ORIGIN
RDNA LOCI
topic CMA/DAPI BANDS
GENOME DONORS
PEANUT GENETIC ORIGIN
RDNA LOCI
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The karyotype structure of Arachis trinitensis was studied by conventional Feulgen staining, CMA/DAPI banding and rDNA loci detection by fluorescence in situ hybridization (FISH) in order to establish its genome status and test the hypothesis that this species is a genome donor of cultivated peanut. Conventional staining revealed that the karyotype lacked the small "A chromosomes" characteristic of the A genome. In agreement with this, chromosomal banding showed that none of the chromosomes had the large centromeric bands expected for A chromosomes. FISH revealed one pair each of 5S and 45S rDNA loci, located in different medium-sized metacentric chromosomes. Collectively, these results suggest that A. trinitensis should be removed from the A genome and be considered as a B or non-A genome species. The pattern of heterochromatic bands and rDNA loci of A. trinitensis differ markedly from any of the complements of A. hypogaea, suggesting that the former species is unlikely to be one of the wild diploid progenitors of the latter. © 2010, Sociedade Brasileira de Genética.
Fil: Robledo Dobladez, Germán Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina
Fil: Lavia, Graciela Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Seijo, José Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina
description The karyotype structure of Arachis trinitensis was studied by conventional Feulgen staining, CMA/DAPI banding and rDNA loci detection by fluorescence in situ hybridization (FISH) in order to establish its genome status and test the hypothesis that this species is a genome donor of cultivated peanut. Conventional staining revealed that the karyotype lacked the small "A chromosomes" characteristic of the A genome. In agreement with this, chromosomal banding showed that none of the chromosomes had the large centromeric bands expected for A chromosomes. FISH revealed one pair each of 5S and 45S rDNA loci, located in different medium-sized metacentric chromosomes. Collectively, these results suggest that A. trinitensis should be removed from the A genome and be considered as a B or non-A genome species. The pattern of heterochromatic bands and rDNA loci of A. trinitensis differ markedly from any of the complements of A. hypogaea, suggesting that the former species is unlikely to be one of the wild diploid progenitors of the latter. © 2010, Sociedade Brasileira de Genética.
publishDate 2010
dc.date.none.fl_str_mv 2010-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/66061
Robledo Dobladez, Germán Ariel; Lavia, Graciela Inés; Seijo, José Guillermo; Genome re-assignment of Arachis trinitensis (sect. Arachis, Leguminosae) and its implications for the genetic origin of cultivated peanut; Sociedade Brasileira de Genética; Genetics and Molecular Biology; 33; 4; 4-2010; 714-718
1415-4757
1678-4685
CONICET Digital
CONICET
url http://hdl.handle.net/11336/66061
identifier_str_mv Robledo Dobladez, Germán Ariel; Lavia, Graciela Inés; Seijo, José Guillermo; Genome re-assignment of Arachis trinitensis (sect. Arachis, Leguminosae) and its implications for the genetic origin of cultivated peanut; Sociedade Brasileira de Genética; Genetics and Molecular Biology; 33; 4; 4-2010; 714-718
1415-4757
1678-4685
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1590/S1415-47572010005000079
info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/49dych
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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