Discontinuous versus continuous drinking of ethanol in peripubertal rats: Effect on 24-hour pattern of hypophyseal-gonadal axis activity and anterior pituitary oxidative stress

Autores
Fernández Mateos, Pilar; Jiménez Ortega, Vanesa; Cano Barquilla, Pilar; Cardinali, Daniel Pedro; Esquifino, Ana I.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aims: Discontinuous (weekend) consumption of alcohol is common in adolescents and young adults. This study therefore assesses, in peripubertal male rats, the effect of discontinuous as compared to chronic feeding of ethanol or control liquid diet. Methods: Animals received an ethanol liquid diet (6.2 % w/v) starting on day 35 of life. Every week for 5 weeks, the discontinuous ethanol group received the ethanol diet for 3 consecutive days and the control liquid diet for 4 days. At the 5th week, 24 h after the last ethanol administration to the discontinuously ethanol-treated animals, rats were killed at 4-hour intervals beginning at 09.00 h. Chronically administered rats received the ethanol diet until immediately before study. Results: Disrupted 24-hour rhythmicity together with a significant nocturnal increase in plasma luteinizing hormone (LH), testosterone and prolactin (PRL) occurred in the discontinuous ethanol group. Plasma ethanol levels were undetectable at 24 h after the last ethanol treatment. In contrast, after chronic ethanol administration, plasma PRL was increased late in scotophase while LH and testosterone decreased; blood ethanol levels were 2-fold greater than those in discontinuously ethanol-administered rats killed immediately after ethanol withdrawal. Circulating testosterone positively correlated with LH levels in control rats only. Chronic administration of ethanol significantly augmented mean expression of pituitary nitric oxide synthase (NOS)-2, heme oxygenase (HO)-1, Per1 and Per2 genes and disrupted their diurnal rhythmicity. Decreased NOS-1 and NOS-2 expression during scotophase, together with suppression of the rhythm in Per1 and Per2 expression, were found in the discontinuous ethanol group. Conclusions: Abstinence after discontinuous drinking of alcohol in rats, as compared to chronic administration of ethanol, is accompanied by increases of plasma LH and testosterone, a greater PRL response and a less pronounced oxidative damage of the anterior pituitary.
Fil: Fernández Mateos, Pilar. Universidad Complutense de Madrid; España
Fil: Jiménez Ortega, Vanesa. Universidad Complutense de Madrid; España
Fil: Cano Barquilla, Pilar. Universidad Complutense de Madrid; España
Fil: Cardinali, Daniel Pedro. Universidad de Buenos Aires; Argentina. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Esquifino, Ana I.. Universidad Complutense de Madrid; España
Materia
ALCOHOLISM
ANTERIOR PITUITARY
CHRONIC ALCOHOL
CLOCK GENES
HYPOTHALAMIC-PITUITARY-GONADAL AXIS
LIBIDO
OXIDATIVE STRESS
PROLACTIN
REPRODUCTION
THYROID-STIMULATING HORMONE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/194450
Acceder
id CONICETDig_8d46abb9baee5cabcaa1573e6db4f2cd
oai_identifier_str oai:ri.conicet.gov.ar:11336/194450
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Discontinuous versus continuous drinking of ethanol in peripubertal rats: Effect on 24-hour pattern of hypophyseal-gonadal axis activity and anterior pituitary oxidative stressFernández Mateos, PilarJiménez Ortega, VanesaCano Barquilla, PilarCardinali, Daniel PedroEsquifino, Ana I.ALCOHOLISMANTERIOR PITUITARYCHRONIC ALCOHOLCLOCK GENESHYPOTHALAMIC-PITUITARY-GONADAL AXISLIBIDOOXIDATIVE STRESSPROLACTINREPRODUCTIONTHYROID-STIMULATING HORMONEhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Aims: Discontinuous (weekend) consumption of alcohol is common in adolescents and young adults. This study therefore assesses, in peripubertal male rats, the effect of discontinuous as compared to chronic feeding of ethanol or control liquid diet. Methods: Animals received an ethanol liquid diet (6.2 % w/v) starting on day 35 of life. Every week for 5 weeks, the discontinuous ethanol group received the ethanol diet for 3 consecutive days and the control liquid diet for 4 days. At the 5th week, 24 h after the last ethanol administration to the discontinuously ethanol-treated animals, rats were killed at 4-hour intervals beginning at 09.00 h. Chronically administered rats received the ethanol diet until immediately before study. Results: Disrupted 24-hour rhythmicity together with a significant nocturnal increase in plasma luteinizing hormone (LH), testosterone and prolactin (PRL) occurred in the discontinuous ethanol group. Plasma ethanol levels were undetectable at 24 h after the last ethanol treatment. In contrast, after chronic ethanol administration, plasma PRL was increased late in scotophase while LH and testosterone decreased; blood ethanol levels were 2-fold greater than those in discontinuously ethanol-administered rats killed immediately after ethanol withdrawal. Circulating testosterone positively correlated with LH levels in control rats only. Chronic administration of ethanol significantly augmented mean expression of pituitary nitric oxide synthase (NOS)-2, heme oxygenase (HO)-1, Per1 and Per2 genes and disrupted their diurnal rhythmicity. Decreased NOS-1 and NOS-2 expression during scotophase, together with suppression of the rhythm in Per1 and Per2 expression, were found in the discontinuous ethanol group. Conclusions: Abstinence after discontinuous drinking of alcohol in rats, as compared to chronic administration of ethanol, is accompanied by increases of plasma LH and testosterone, a greater PRL response and a less pronounced oxidative damage of the anterior pituitary.Fil: Fernández Mateos, Pilar. Universidad Complutense de Madrid; EspañaFil: Jiménez Ortega, Vanesa. Universidad Complutense de Madrid; EspañaFil: Cano Barquilla, Pilar. Universidad Complutense de Madrid; EspañaFil: Cardinali, Daniel Pedro. Universidad de Buenos Aires; Argentina. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Esquifino, Ana I.. Universidad Complutense de Madrid; EspañaKarger2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/194450Fernández Mateos, Pilar; Jiménez Ortega, Vanesa; Cano Barquilla, Pilar; Cardinali, Daniel Pedro; Esquifino, Ana I.; Discontinuous versus continuous drinking of ethanol in peripubertal rats: Effect on 24-hour pattern of hypophyseal-gonadal axis activity and anterior pituitary oxidative stress; Karger; Neuroendocrinology; 96; 3; 1-2012; 194-2030028-3835CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/Abstract/334963info:eu-repo/semantics/altIdentifier/doi/10.1159/000334963info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:55:59Zoai:ri.conicet.gov.ar:11336/194450instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:55:59.953CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Discontinuous versus continuous drinking of ethanol in peripubertal rats: Effect on 24-hour pattern of hypophyseal-gonadal axis activity and anterior pituitary oxidative stress
title Discontinuous versus continuous drinking of ethanol in peripubertal rats: Effect on 24-hour pattern of hypophyseal-gonadal axis activity and anterior pituitary oxidative stress
spellingShingle Discontinuous versus continuous drinking of ethanol in peripubertal rats: Effect on 24-hour pattern of hypophyseal-gonadal axis activity and anterior pituitary oxidative stress
Fernández Mateos, Pilar
ALCOHOLISM
ANTERIOR PITUITARY
CHRONIC ALCOHOL
CLOCK GENES
HYPOTHALAMIC-PITUITARY-GONADAL AXIS
LIBIDO
OXIDATIVE STRESS
PROLACTIN
REPRODUCTION
THYROID-STIMULATING HORMONE
title_short Discontinuous versus continuous drinking of ethanol in peripubertal rats: Effect on 24-hour pattern of hypophyseal-gonadal axis activity and anterior pituitary oxidative stress
title_full Discontinuous versus continuous drinking of ethanol in peripubertal rats: Effect on 24-hour pattern of hypophyseal-gonadal axis activity and anterior pituitary oxidative stress
title_fullStr Discontinuous versus continuous drinking of ethanol in peripubertal rats: Effect on 24-hour pattern of hypophyseal-gonadal axis activity and anterior pituitary oxidative stress
title_full_unstemmed Discontinuous versus continuous drinking of ethanol in peripubertal rats: Effect on 24-hour pattern of hypophyseal-gonadal axis activity and anterior pituitary oxidative stress
title_sort Discontinuous versus continuous drinking of ethanol in peripubertal rats: Effect on 24-hour pattern of hypophyseal-gonadal axis activity and anterior pituitary oxidative stress
dc.creator.none.fl_str_mv Fernández Mateos, Pilar
Jiménez Ortega, Vanesa
Cano Barquilla, Pilar
Cardinali, Daniel Pedro
Esquifino, Ana I.
author Fernández Mateos, Pilar
author_facet Fernández Mateos, Pilar
Jiménez Ortega, Vanesa
Cano Barquilla, Pilar
Cardinali, Daniel Pedro
Esquifino, Ana I.
author_role author
author2 Jiménez Ortega, Vanesa
Cano Barquilla, Pilar
Cardinali, Daniel Pedro
Esquifino, Ana I.
author2_role author
author
author
author
dc.subject.none.fl_str_mv ALCOHOLISM
ANTERIOR PITUITARY
CHRONIC ALCOHOL
CLOCK GENES
HYPOTHALAMIC-PITUITARY-GONADAL AXIS
LIBIDO
OXIDATIVE STRESS
PROLACTIN
REPRODUCTION
THYROID-STIMULATING HORMONE
topic ALCOHOLISM
ANTERIOR PITUITARY
CHRONIC ALCOHOL
CLOCK GENES
HYPOTHALAMIC-PITUITARY-GONADAL AXIS
LIBIDO
OXIDATIVE STRESS
PROLACTIN
REPRODUCTION
THYROID-STIMULATING HORMONE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Aims: Discontinuous (weekend) consumption of alcohol is common in adolescents and young adults. This study therefore assesses, in peripubertal male rats, the effect of discontinuous as compared to chronic feeding of ethanol or control liquid diet. Methods: Animals received an ethanol liquid diet (6.2 % w/v) starting on day 35 of life. Every week for 5 weeks, the discontinuous ethanol group received the ethanol diet for 3 consecutive days and the control liquid diet for 4 days. At the 5th week, 24 h after the last ethanol administration to the discontinuously ethanol-treated animals, rats were killed at 4-hour intervals beginning at 09.00 h. Chronically administered rats received the ethanol diet until immediately before study. Results: Disrupted 24-hour rhythmicity together with a significant nocturnal increase in plasma luteinizing hormone (LH), testosterone and prolactin (PRL) occurred in the discontinuous ethanol group. Plasma ethanol levels were undetectable at 24 h after the last ethanol treatment. In contrast, after chronic ethanol administration, plasma PRL was increased late in scotophase while LH and testosterone decreased; blood ethanol levels were 2-fold greater than those in discontinuously ethanol-administered rats killed immediately after ethanol withdrawal. Circulating testosterone positively correlated with LH levels in control rats only. Chronic administration of ethanol significantly augmented mean expression of pituitary nitric oxide synthase (NOS)-2, heme oxygenase (HO)-1, Per1 and Per2 genes and disrupted their diurnal rhythmicity. Decreased NOS-1 and NOS-2 expression during scotophase, together with suppression of the rhythm in Per1 and Per2 expression, were found in the discontinuous ethanol group. Conclusions: Abstinence after discontinuous drinking of alcohol in rats, as compared to chronic administration of ethanol, is accompanied by increases of plasma LH and testosterone, a greater PRL response and a less pronounced oxidative damage of the anterior pituitary.
Fil: Fernández Mateos, Pilar. Universidad Complutense de Madrid; España
Fil: Jiménez Ortega, Vanesa. Universidad Complutense de Madrid; España
Fil: Cano Barquilla, Pilar. Universidad Complutense de Madrid; España
Fil: Cardinali, Daniel Pedro. Universidad de Buenos Aires; Argentina. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Esquifino, Ana I.. Universidad Complutense de Madrid; España
description Aims: Discontinuous (weekend) consumption of alcohol is common in adolescents and young adults. This study therefore assesses, in peripubertal male rats, the effect of discontinuous as compared to chronic feeding of ethanol or control liquid diet. Methods: Animals received an ethanol liquid diet (6.2 % w/v) starting on day 35 of life. Every week for 5 weeks, the discontinuous ethanol group received the ethanol diet for 3 consecutive days and the control liquid diet for 4 days. At the 5th week, 24 h after the last ethanol administration to the discontinuously ethanol-treated animals, rats were killed at 4-hour intervals beginning at 09.00 h. Chronically administered rats received the ethanol diet until immediately before study. Results: Disrupted 24-hour rhythmicity together with a significant nocturnal increase in plasma luteinizing hormone (LH), testosterone and prolactin (PRL) occurred in the discontinuous ethanol group. Plasma ethanol levels were undetectable at 24 h after the last ethanol treatment. In contrast, after chronic ethanol administration, plasma PRL was increased late in scotophase while LH and testosterone decreased; blood ethanol levels were 2-fold greater than those in discontinuously ethanol-administered rats killed immediately after ethanol withdrawal. Circulating testosterone positively correlated with LH levels in control rats only. Chronic administration of ethanol significantly augmented mean expression of pituitary nitric oxide synthase (NOS)-2, heme oxygenase (HO)-1, Per1 and Per2 genes and disrupted their diurnal rhythmicity. Decreased NOS-1 and NOS-2 expression during scotophase, together with suppression of the rhythm in Per1 and Per2 expression, were found in the discontinuous ethanol group. Conclusions: Abstinence after discontinuous drinking of alcohol in rats, as compared to chronic administration of ethanol, is accompanied by increases of plasma LH and testosterone, a greater PRL response and a less pronounced oxidative damage of the anterior pituitary.
publishDate 2012
dc.date.none.fl_str_mv 2012-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/194450
Fernández Mateos, Pilar; Jiménez Ortega, Vanesa; Cano Barquilla, Pilar; Cardinali, Daniel Pedro; Esquifino, Ana I.; Discontinuous versus continuous drinking of ethanol in peripubertal rats: Effect on 24-hour pattern of hypophyseal-gonadal axis activity and anterior pituitary oxidative stress; Karger; Neuroendocrinology; 96; 3; 1-2012; 194-203
0028-3835
CONICET Digital
CONICET
url http://hdl.handle.net/11336/194450
identifier_str_mv Fernández Mateos, Pilar; Jiménez Ortega, Vanesa; Cano Barquilla, Pilar; Cardinali, Daniel Pedro; Esquifino, Ana I.; Discontinuous versus continuous drinking of ethanol in peripubertal rats: Effect on 24-hour pattern of hypophyseal-gonadal axis activity and anterior pituitary oxidative stress; Karger; Neuroendocrinology; 96; 3; 1-2012; 194-203
0028-3835
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/Abstract/334963
info:eu-repo/semantics/altIdentifier/doi/10.1159/000334963
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269376479232000
score 13.13397

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