Casein Glycomacropeptide Regulates Gene Expression in Intestinal Epithelial Cells: Effect of Simulated Gastrointestinal Digestion and Peptide Microencapsulation
- Autores
- Cian, Raúl Esteban; Tena Garitaonaindia, Mireia; Sánchez de Medina, Fermín; Martínez Augustin, Olga
- Año de publicación
- 2025
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- κ-Casein glycomacropeptide (GMP) exerts anti-inflammatory and immune modulatory effects. A bovine GMP concentrate and its in vitro digestion product were obtained. GMP was also microencapsulated with phycocolloids and further digested. These products were tested in three-dimensional (3D) and open monolayer two-dimensional (2D) mouse jejunal organoids. Almost no effect was observed on the 2D organoids. In 3D organoids, GMP induced intestinal proliferation (Axin2, Pcna) and differentiation (Vil1, Alpl) genes together with Muc3, antibacterial genes (Lyz1, Pla2g2a), and Cxcl1. GMP also induced interferon I defense genes (Ifnb1, Ifr3, Oas2, Oas3, Rnasel) under basal conditions and in TNF-stimulated organoids. In vitro digestion abrogated the effects of GMP and induced new genes (Lgr5, Olfm4, and Lct). In TNF-stimulated organoids, digested GMP repressed multiple genes. Encapsulation largely preserved the GMP effects. In conclusion, GMP showed differential effects in 3D and 2D organoids. The effects of digestion peptides were also different, suggesting distinct potential as functional foods.
Fil: Cian, Raúl Esteban. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Tena Garitaonaindia, Mireia. Universidad de Granada; España
Fil: Sánchez de Medina, Fermín. Universidad de Granada; España
Fil: Martínez Augustin, Olga. Universidad de Granada; España - Materia
-
CASEIN GLYCOMACROPEPTIDE
INTESTINAL EPITHELIAL CELLS
INTESTINAL ORGANOIDS
IN VITRO GASTROINTESTINAL DIGESTION
DIALYZABILITY
ENCAPSULATION BY SPRAY DRYING - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/272632
Ver los metadatos del registro completo
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CONICET Digital (CONICET) |
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Casein Glycomacropeptide Regulates Gene Expression in Intestinal Epithelial Cells: Effect of Simulated Gastrointestinal Digestion and Peptide MicroencapsulationCian, Raúl EstebanTena Garitaonaindia, MireiaSánchez de Medina, FermínMartínez Augustin, OlgaCASEIN GLYCOMACROPEPTIDEINTESTINAL EPITHELIAL CELLSINTESTINAL ORGANOIDSIN VITRO GASTROINTESTINAL DIGESTIONDIALYZABILITYENCAPSULATION BY SPRAY DRYINGhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2κ-Casein glycomacropeptide (GMP) exerts anti-inflammatory and immune modulatory effects. A bovine GMP concentrate and its in vitro digestion product were obtained. GMP was also microencapsulated with phycocolloids and further digested. These products were tested in three-dimensional (3D) and open monolayer two-dimensional (2D) mouse jejunal organoids. Almost no effect was observed on the 2D organoids. In 3D organoids, GMP induced intestinal proliferation (Axin2, Pcna) and differentiation (Vil1, Alpl) genes together with Muc3, antibacterial genes (Lyz1, Pla2g2a), and Cxcl1. GMP also induced interferon I defense genes (Ifnb1, Ifr3, Oas2, Oas3, Rnasel) under basal conditions and in TNF-stimulated organoids. In vitro digestion abrogated the effects of GMP and induced new genes (Lgr5, Olfm4, and Lct). In TNF-stimulated organoids, digested GMP repressed multiple genes. Encapsulation largely preserved the GMP effects. In conclusion, GMP showed differential effects in 3D and 2D organoids. The effects of digestion peptides were also different, suggesting distinct potential as functional foods.Fil: Cian, Raúl Esteban. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Tena Garitaonaindia, Mireia. Universidad de Granada; EspañaFil: Sánchez de Medina, Fermín. Universidad de Granada; EspañaFil: Martínez Augustin, Olga. Universidad de Granada; EspañaAmerican Chemical Society2025-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/272632Cian, Raúl Esteban; Tena Garitaonaindia, Mireia; Sánchez de Medina, Fermín; Martínez Augustin, Olga; Casein Glycomacropeptide Regulates Gene Expression in Intestinal Epithelial Cells: Effect of Simulated Gastrointestinal Digestion and Peptide Microencapsulation; American Chemical Society; Journal of Agricultural and Food Chemistry; 73; 7; 2-2025; 4105-41150021-8561CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/acs.jafc.4c10146info:eu-repo/semantics/altIdentifier/doi/10.1021/acs.jafc.4c10146info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:56:03Zoai:ri.conicet.gov.ar:11336/272632instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:56:03.822CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Casein Glycomacropeptide Regulates Gene Expression in Intestinal Epithelial Cells: Effect of Simulated Gastrointestinal Digestion and Peptide Microencapsulation |
title |
Casein Glycomacropeptide Regulates Gene Expression in Intestinal Epithelial Cells: Effect of Simulated Gastrointestinal Digestion and Peptide Microencapsulation |
spellingShingle |
Casein Glycomacropeptide Regulates Gene Expression in Intestinal Epithelial Cells: Effect of Simulated Gastrointestinal Digestion and Peptide Microencapsulation Cian, Raúl Esteban CASEIN GLYCOMACROPEPTIDE INTESTINAL EPITHELIAL CELLS INTESTINAL ORGANOIDS IN VITRO GASTROINTESTINAL DIGESTION DIALYZABILITY ENCAPSULATION BY SPRAY DRYING |
title_short |
Casein Glycomacropeptide Regulates Gene Expression in Intestinal Epithelial Cells: Effect of Simulated Gastrointestinal Digestion and Peptide Microencapsulation |
title_full |
Casein Glycomacropeptide Regulates Gene Expression in Intestinal Epithelial Cells: Effect of Simulated Gastrointestinal Digestion and Peptide Microencapsulation |
title_fullStr |
Casein Glycomacropeptide Regulates Gene Expression in Intestinal Epithelial Cells: Effect of Simulated Gastrointestinal Digestion and Peptide Microencapsulation |
title_full_unstemmed |
Casein Glycomacropeptide Regulates Gene Expression in Intestinal Epithelial Cells: Effect of Simulated Gastrointestinal Digestion and Peptide Microencapsulation |
title_sort |
Casein Glycomacropeptide Regulates Gene Expression in Intestinal Epithelial Cells: Effect of Simulated Gastrointestinal Digestion and Peptide Microencapsulation |
dc.creator.none.fl_str_mv |
Cian, Raúl Esteban Tena Garitaonaindia, Mireia Sánchez de Medina, Fermín Martínez Augustin, Olga |
author |
Cian, Raúl Esteban |
author_facet |
Cian, Raúl Esteban Tena Garitaonaindia, Mireia Sánchez de Medina, Fermín Martínez Augustin, Olga |
author_role |
author |
author2 |
Tena Garitaonaindia, Mireia Sánchez de Medina, Fermín Martínez Augustin, Olga |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
CASEIN GLYCOMACROPEPTIDE INTESTINAL EPITHELIAL CELLS INTESTINAL ORGANOIDS IN VITRO GASTROINTESTINAL DIGESTION DIALYZABILITY ENCAPSULATION BY SPRAY DRYING |
topic |
CASEIN GLYCOMACROPEPTIDE INTESTINAL EPITHELIAL CELLS INTESTINAL ORGANOIDS IN VITRO GASTROINTESTINAL DIGESTION DIALYZABILITY ENCAPSULATION BY SPRAY DRYING |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.11 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
κ-Casein glycomacropeptide (GMP) exerts anti-inflammatory and immune modulatory effects. A bovine GMP concentrate and its in vitro digestion product were obtained. GMP was also microencapsulated with phycocolloids and further digested. These products were tested in three-dimensional (3D) and open monolayer two-dimensional (2D) mouse jejunal organoids. Almost no effect was observed on the 2D organoids. In 3D organoids, GMP induced intestinal proliferation (Axin2, Pcna) and differentiation (Vil1, Alpl) genes together with Muc3, antibacterial genes (Lyz1, Pla2g2a), and Cxcl1. GMP also induced interferon I defense genes (Ifnb1, Ifr3, Oas2, Oas3, Rnasel) under basal conditions and in TNF-stimulated organoids. In vitro digestion abrogated the effects of GMP and induced new genes (Lgr5, Olfm4, and Lct). In TNF-stimulated organoids, digested GMP repressed multiple genes. Encapsulation largely preserved the GMP effects. In conclusion, GMP showed differential effects in 3D and 2D organoids. The effects of digestion peptides were also different, suggesting distinct potential as functional foods. Fil: Cian, Raúl Esteban. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina Fil: Tena Garitaonaindia, Mireia. Universidad de Granada; España Fil: Sánchez de Medina, Fermín. Universidad de Granada; España Fil: Martínez Augustin, Olga. Universidad de Granada; España |
description |
κ-Casein glycomacropeptide (GMP) exerts anti-inflammatory and immune modulatory effects. A bovine GMP concentrate and its in vitro digestion product were obtained. GMP was also microencapsulated with phycocolloids and further digested. These products were tested in three-dimensional (3D) and open monolayer two-dimensional (2D) mouse jejunal organoids. Almost no effect was observed on the 2D organoids. In 3D organoids, GMP induced intestinal proliferation (Axin2, Pcna) and differentiation (Vil1, Alpl) genes together with Muc3, antibacterial genes (Lyz1, Pla2g2a), and Cxcl1. GMP also induced interferon I defense genes (Ifnb1, Ifr3, Oas2, Oas3, Rnasel) under basal conditions and in TNF-stimulated organoids. In vitro digestion abrogated the effects of GMP and induced new genes (Lgr5, Olfm4, and Lct). In TNF-stimulated organoids, digested GMP repressed multiple genes. Encapsulation largely preserved the GMP effects. In conclusion, GMP showed differential effects in 3D and 2D organoids. The effects of digestion peptides were also different, suggesting distinct potential as functional foods. |
publishDate |
2025 |
dc.date.none.fl_str_mv |
2025-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/272632 Cian, Raúl Esteban; Tena Garitaonaindia, Mireia; Sánchez de Medina, Fermín; Martínez Augustin, Olga; Casein Glycomacropeptide Regulates Gene Expression in Intestinal Epithelial Cells: Effect of Simulated Gastrointestinal Digestion and Peptide Microencapsulation; American Chemical Society; Journal of Agricultural and Food Chemistry; 73; 7; 2-2025; 4105-4115 0021-8561 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/272632 |
identifier_str_mv |
Cian, Raúl Esteban; Tena Garitaonaindia, Mireia; Sánchez de Medina, Fermín; Martínez Augustin, Olga; Casein Glycomacropeptide Regulates Gene Expression in Intestinal Epithelial Cells: Effect of Simulated Gastrointestinal Digestion and Peptide Microencapsulation; American Chemical Society; Journal of Agricultural and Food Chemistry; 73; 7; 2-2025; 4105-4115 0021-8561 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/acs.jafc.4c10146 info:eu-repo/semantics/altIdentifier/doi/10.1021/acs.jafc.4c10146 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
American Chemical Society |
publisher.none.fl_str_mv |
American Chemical Society |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1846083096606146560 |
score |
13.22299 |