Localization and distribution of CCK-8‑, NPY‑, Leu-ENK-, and Ghrelin- in the digestive tract of prochilodus lineatus (Valenciennes, 1836)

Autores
Barrios, Carlos Eduardo; Santinón, Juan José; Domitrovic, Hugo Alberto; Sánchez, Sebastián; Hernandez, David Roque
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study describes the histological characteristics and distribution of gastrointestinal tract endocrine cells (ECs) of Prochilodus lineatus (detritivorous fish) using immunohistochemical procedures. The digestive tract of P. lineatus was divided into seven portions: stomach (cardial and pyloric), pyloric caeca, and intestine (anterior, glandular, middle and posterior). A pool of specific antisera against cholecystokinin (CCK-8), -neuropeptide Y (NPY), -ghrelin (Ghre) and -leu-enkephalin (Leu-ENK) to identify ECs were used. According to the morphological characteristics of ECs, two different types were identified and classified as open or closed-type. The number of ECs varied throughout the gastrointestinal tract, though a high abundance was found in the anterior intestine and pyloric caeca. A large number of ECs immunoreactive to CCK-8 and NPY were recorded in the anterior, glandular and middle intestine. ECs immunopositive to Leu-ENK were distributed in the stomach and pyloric caeca. For Ghre, immunopositive ECs were restricted to the glandular intestine. The results of the present study indicate that P. lineatus presents an ECs distribution pattern with species-specific particularities. However, CCK showed a distribution similar to that of omnivores, which is possibly related to local signaling functions in order to achieve the correct digestion of the various organisms found in the detritus.
Fil: Barrios, Carlos Eduardo. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Santinón, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina
Fil: Domitrovic, Hugo Alberto. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Sánchez, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina
Fil: Hernandez, David Roque. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Materia
DETRITIVOROUS FISH
SÁBALO
ENDOCRINE CELLS
IMMUNOHISTOCHEMISTRY
NEUROPEPTIDE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/143503
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/143503
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Localization and distribution of CCK-8‑, NPY‑, Leu-ENK-, and Ghrelin- in the digestive tract of prochilodus lineatus (Valenciennes, 1836)Barrios, Carlos EduardoSantinón, Juan JoséDomitrovic, Hugo AlbertoSánchez, SebastiánHernandez, David RoqueDETRITIVOROUS FISHSÁBALOENDOCRINE CELLSIMMUNOHISTOCHEMISTRYNEUROPEPTIDEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1This study describes the histological characteristics and distribution of gastrointestinal tract endocrine cells (ECs) of Prochilodus lineatus (detritivorous fish) using immunohistochemical procedures. The digestive tract of P. lineatus was divided into seven portions: stomach (cardial and pyloric), pyloric caeca, and intestine (anterior, glandular, middle and posterior). A pool of specific antisera against cholecystokinin (CCK-8), -neuropeptide Y (NPY), -ghrelin (Ghre) and -leu-enkephalin (Leu-ENK) to identify ECs were used. According to the morphological characteristics of ECs, two different types were identified and classified as open or closed-type. The number of ECs varied throughout the gastrointestinal tract, though a high abundance was found in the anterior intestine and pyloric caeca. A large number of ECs immunoreactive to CCK-8 and NPY were recorded in the anterior, glandular and middle intestine. ECs immunopositive to Leu-ENK were distributed in the stomach and pyloric caeca. For Ghre, immunopositive ECs were restricted to the glandular intestine. The results of the present study indicate that P. lineatus presents an ECs distribution pattern with species-specific particularities. However, CCK showed a distribution similar to that of omnivores, which is possibly related to local signaling functions in order to achieve the correct digestion of the various organisms found in the detritus.Fil: Barrios, Carlos Eduardo. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Santinón, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; ArgentinaFil: Domitrovic, Hugo Alberto. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Sánchez, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; ArgentinaFil: Hernandez, David Roque. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaAcademia Brasileira de Ciencias2020-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/143503Barrios, Carlos Eduardo; Santinón, Juan José; Domitrovic, Hugo Alberto; Sánchez, Sebastián; Hernandez, David Roque; Localization and distribution of CCK-8‑, NPY‑, Leu-ENK-, and Ghrelin- in the digestive tract of prochilodus lineatus (Valenciennes, 1836); Academia Brasileira de Ciencias; Anais da Academia Brasileira de Ciencias; 92; 2; 12-2020; 1-120001-37651678-2690CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.scielo.br/j/aabc/a/5W59vTKpwvvyjgXwCGyfYjP/?lang=eninfo:eu-repo/semantics/altIdentifier/doi/10.1590/0001-3765202020181165info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:00:01Zoai:ri.conicet.gov.ar:11336/143503instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:00:01.441CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Localization and distribution of CCK-8‑, NPY‑, Leu-ENK-, and Ghrelin- in the digestive tract of prochilodus lineatus (Valenciennes, 1836)
title Localization and distribution of CCK-8‑, NPY‑, Leu-ENK-, and Ghrelin- in the digestive tract of prochilodus lineatus (Valenciennes, 1836)
spellingShingle Localization and distribution of CCK-8‑, NPY‑, Leu-ENK-, and Ghrelin- in the digestive tract of prochilodus lineatus (Valenciennes, 1836)
Barrios, Carlos Eduardo
DETRITIVOROUS FISH
SÁBALO
ENDOCRINE CELLS
IMMUNOHISTOCHEMISTRY
NEUROPEPTIDE
title_short Localization and distribution of CCK-8‑, NPY‑, Leu-ENK-, and Ghrelin- in the digestive tract of prochilodus lineatus (Valenciennes, 1836)
title_full Localization and distribution of CCK-8‑, NPY‑, Leu-ENK-, and Ghrelin- in the digestive tract of prochilodus lineatus (Valenciennes, 1836)
title_fullStr Localization and distribution of CCK-8‑, NPY‑, Leu-ENK-, and Ghrelin- in the digestive tract of prochilodus lineatus (Valenciennes, 1836)
title_full_unstemmed Localization and distribution of CCK-8‑, NPY‑, Leu-ENK-, and Ghrelin- in the digestive tract of prochilodus lineatus (Valenciennes, 1836)
title_sort Localization and distribution of CCK-8‑, NPY‑, Leu-ENK-, and Ghrelin- in the digestive tract of prochilodus lineatus (Valenciennes, 1836)
dc.creator.none.fl_str_mv Barrios, Carlos Eduardo
Santinón, Juan José
Domitrovic, Hugo Alberto
Sánchez, Sebastián
Hernandez, David Roque
author Barrios, Carlos Eduardo
author_facet Barrios, Carlos Eduardo
Santinón, Juan José
Domitrovic, Hugo Alberto
Sánchez, Sebastián
Hernandez, David Roque
author_role author
author2 Santinón, Juan José
Domitrovic, Hugo Alberto
Sánchez, Sebastián
Hernandez, David Roque
author2_role author
author
author
author
dc.subject.none.fl_str_mv DETRITIVOROUS FISH
SÁBALO
ENDOCRINE CELLS
IMMUNOHISTOCHEMISTRY
NEUROPEPTIDE
topic DETRITIVOROUS FISH
SÁBALO
ENDOCRINE CELLS
IMMUNOHISTOCHEMISTRY
NEUROPEPTIDE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This study describes the histological characteristics and distribution of gastrointestinal tract endocrine cells (ECs) of Prochilodus lineatus (detritivorous fish) using immunohistochemical procedures. The digestive tract of P. lineatus was divided into seven portions: stomach (cardial and pyloric), pyloric caeca, and intestine (anterior, glandular, middle and posterior). A pool of specific antisera against cholecystokinin (CCK-8), -neuropeptide Y (NPY), -ghrelin (Ghre) and -leu-enkephalin (Leu-ENK) to identify ECs were used. According to the morphological characteristics of ECs, two different types were identified and classified as open or closed-type. The number of ECs varied throughout the gastrointestinal tract, though a high abundance was found in the anterior intestine and pyloric caeca. A large number of ECs immunoreactive to CCK-8 and NPY were recorded in the anterior, glandular and middle intestine. ECs immunopositive to Leu-ENK were distributed in the stomach and pyloric caeca. For Ghre, immunopositive ECs were restricted to the glandular intestine. The results of the present study indicate that P. lineatus presents an ECs distribution pattern with species-specific particularities. However, CCK showed a distribution similar to that of omnivores, which is possibly related to local signaling functions in order to achieve the correct digestion of the various organisms found in the detritus.
Fil: Barrios, Carlos Eduardo. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Santinón, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina
Fil: Domitrovic, Hugo Alberto. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Sánchez, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina
Fil: Hernandez, David Roque. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
description This study describes the histological characteristics and distribution of gastrointestinal tract endocrine cells (ECs) of Prochilodus lineatus (detritivorous fish) using immunohistochemical procedures. The digestive tract of P. lineatus was divided into seven portions: stomach (cardial and pyloric), pyloric caeca, and intestine (anterior, glandular, middle and posterior). A pool of specific antisera against cholecystokinin (CCK-8), -neuropeptide Y (NPY), -ghrelin (Ghre) and -leu-enkephalin (Leu-ENK) to identify ECs were used. According to the morphological characteristics of ECs, two different types were identified and classified as open or closed-type. The number of ECs varied throughout the gastrointestinal tract, though a high abundance was found in the anterior intestine and pyloric caeca. A large number of ECs immunoreactive to CCK-8 and NPY were recorded in the anterior, glandular and middle intestine. ECs immunopositive to Leu-ENK were distributed in the stomach and pyloric caeca. For Ghre, immunopositive ECs were restricted to the glandular intestine. The results of the present study indicate that P. lineatus presents an ECs distribution pattern with species-specific particularities. However, CCK showed a distribution similar to that of omnivores, which is possibly related to local signaling functions in order to achieve the correct digestion of the various organisms found in the detritus.
publishDate 2020
dc.date.none.fl_str_mv 2020-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/143503
Barrios, Carlos Eduardo; Santinón, Juan José; Domitrovic, Hugo Alberto; Sánchez, Sebastián; Hernandez, David Roque; Localization and distribution of CCK-8‑, NPY‑, Leu-ENK-, and Ghrelin- in the digestive tract of prochilodus lineatus (Valenciennes, 1836); Academia Brasileira de Ciencias; Anais da Academia Brasileira de Ciencias; 92; 2; 12-2020; 1-12
0001-3765
1678-2690
CONICET Digital
CONICET
url http://hdl.handle.net/11336/143503
identifier_str_mv Barrios, Carlos Eduardo; Santinón, Juan José; Domitrovic, Hugo Alberto; Sánchez, Sebastián; Hernandez, David Roque; Localization and distribution of CCK-8‑, NPY‑, Leu-ENK-, and Ghrelin- in the digestive tract of prochilodus lineatus (Valenciennes, 1836); Academia Brasileira de Ciencias; Anais da Academia Brasileira de Ciencias; 92; 2; 12-2020; 1-12
0001-3765
1678-2690
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.scielo.br/j/aabc/a/5W59vTKpwvvyjgXwCGyfYjP/?lang=en
info:eu-repo/semantics/altIdentifier/doi/10.1590/0001-3765202020181165
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academia Brasileira de Ciencias
publisher.none.fl_str_mv Academia Brasileira de Ciencias
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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