Activation of Limulus Coagulation Factor G by Scleroglucan as a Biological Activity Measurement

Autores
Viñarta, Silvana Carolina; Virgili Alemán, Inés María; Castellanos, Lucia Ines; Fariña, Julia Ines
Año de publicación
2010
Idioma
español castellano
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The ability of scleroglucan (beta-1,3-beta-1,6 glucan) conformers to activate Limulus coagulation factor G was evaluated. Studies pursued to get a further insight into the scleroglucan structure-function relationship. Lab-scale produced (EPS I, EPS II, EPSi) and commercial (LSCL) scleroglucans were tested on their ability to activate factor G of the Limulus coagulation cascade (Glucatell Test, Pyrolab). Native triplex conformation was obtained by dissolving scleroglucan (2 pg/mL) in distilled water. Thermally- (150°C, 30 min) and alkali-treated (0·2 N NaOH, 10 min) scleroglucans were also prepared. Single helices were further evaluated at 2 ng/mL and 2 µg/mL. All scleroglucans were endotoxin-free (LAL-Test, Pyrolab). Triplex conformation had stronger ability to activate factor G than single helix, with EPS I and EPS II as the most active polymers. Thermal and alkaline denaturation significantly reduced scleroglucan reactivity, being EPS II the most stable glucan, whilst EPSi and LSCL were the most affected ones (91-99% reduction). Single helix activation ability was significantly dependent on polysaccharide concentration. Scleroglucans from S. rolfsii ATCC 201126 (EPS I and EPS II) exhibited marked clotting activity against factor G and were more effective than commercial scleroglucan. Denaturation would lead to lower biological activity and it would be dependent on EPS concentration.
Fil: Viñarta, Silvana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Virgili Alemán, Inés María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XLVI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Chubut
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Materia
ESCLEROGLUCANO
LIMULUS TEST
CONFORMACION
ACTIVIDAD BIOLOGICA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/214198
Acceder
id CONICETDig_8c7d24a27f16ee14493872e14ea0e4ec
oai_identifier_str oai:ri.conicet.gov.ar:11336/214198
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Activation of Limulus Coagulation Factor G by Scleroglucan as a Biological Activity MeasurementViñarta, Silvana CarolinaVirgili Alemán, Inés MaríaCastellanos, Lucia InesFariña, Julia InesESCLEROGLUCANOLIMULUS TESTCONFORMACIONACTIVIDAD BIOLOGICAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The ability of scleroglucan (beta-1,3-beta-1,6 glucan) conformers to activate Limulus coagulation factor G was evaluated. Studies pursued to get a further insight into the scleroglucan structure-function relationship. Lab-scale produced (EPS I, EPS II, EPSi) and commercial (LSCL) scleroglucans were tested on their ability to activate factor G of the Limulus coagulation cascade (Glucatell Test, Pyrolab). Native triplex conformation was obtained by dissolving scleroglucan (2 pg/mL) in distilled water. Thermally- (150°C, 30 min) and alkali-treated (0·2 N NaOH, 10 min) scleroglucans were also prepared. Single helices were further evaluated at 2 ng/mL and 2 µg/mL. All scleroglucans were endotoxin-free (LAL-Test, Pyrolab). Triplex conformation had stronger ability to activate factor G than single helix, with EPS I and EPS II as the most active polymers. Thermal and alkaline denaturation significantly reduced scleroglucan reactivity, being EPS II the most stable glucan, whilst EPSi and LSCL were the most affected ones (91-99% reduction). Single helix activation ability was significantly dependent on polysaccharide concentration. Scleroglucans from S. rolfsii ATCC 201126 (EPS I and EPS II) exhibited marked clotting activity against factor G and were more effective than commercial scleroglucan. Denaturation would lead to lower biological activity and it would be dependent on EPS concentration.Fil: Viñarta, Silvana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Virgili Alemán, Inés María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaXLVI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología MolecularChubutArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularTech Science Press2010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/214198Activation of Limulus Coagulation Factor G by Scleroglucan as a Biological Activity Measurement; XLVI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Chubut; Argentina; 2010; 98-980327-9545CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/http://saib.org.ar/archivos/biocell-34.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-01-14T12:01:53Zoai:ri.conicet.gov.ar:11336/214198instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-01-14 12:01:53.53CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Activation of Limulus Coagulation Factor G by Scleroglucan as a Biological Activity Measurement
title Activation of Limulus Coagulation Factor G by Scleroglucan as a Biological Activity Measurement
spellingShingle Activation of Limulus Coagulation Factor G by Scleroglucan as a Biological Activity Measurement
Viñarta, Silvana Carolina
ESCLEROGLUCANO
LIMULUS TEST
CONFORMACION
ACTIVIDAD BIOLOGICA
title_short Activation of Limulus Coagulation Factor G by Scleroglucan as a Biological Activity Measurement
title_full Activation of Limulus Coagulation Factor G by Scleroglucan as a Biological Activity Measurement
title_fullStr Activation of Limulus Coagulation Factor G by Scleroglucan as a Biological Activity Measurement
title_full_unstemmed Activation of Limulus Coagulation Factor G by Scleroglucan as a Biological Activity Measurement
title_sort Activation of Limulus Coagulation Factor G by Scleroglucan as a Biological Activity Measurement
dc.creator.none.fl_str_mv Viñarta, Silvana Carolina
Virgili Alemán, Inés María
Castellanos, Lucia Ines
Fariña, Julia Ines
author Viñarta, Silvana Carolina
author_facet Viñarta, Silvana Carolina
Virgili Alemán, Inés María
Castellanos, Lucia Ines
Fariña, Julia Ines
author_role author
author2 Virgili Alemán, Inés María
Castellanos, Lucia Ines
Fariña, Julia Ines
author2_role author
author
author
dc.subject.none.fl_str_mv ESCLEROGLUCANO
LIMULUS TEST
CONFORMACION
ACTIVIDAD BIOLOGICA
topic ESCLEROGLUCANO
LIMULUS TEST
CONFORMACION
ACTIVIDAD BIOLOGICA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The ability of scleroglucan (beta-1,3-beta-1,6 glucan) conformers to activate Limulus coagulation factor G was evaluated. Studies pursued to get a further insight into the scleroglucan structure-function relationship. Lab-scale produced (EPS I, EPS II, EPSi) and commercial (LSCL) scleroglucans were tested on their ability to activate factor G of the Limulus coagulation cascade (Glucatell Test, Pyrolab). Native triplex conformation was obtained by dissolving scleroglucan (2 pg/mL) in distilled water. Thermally- (150°C, 30 min) and alkali-treated (0·2 N NaOH, 10 min) scleroglucans were also prepared. Single helices were further evaluated at 2 ng/mL and 2 µg/mL. All scleroglucans were endotoxin-free (LAL-Test, Pyrolab). Triplex conformation had stronger ability to activate factor G than single helix, with EPS I and EPS II as the most active polymers. Thermal and alkaline denaturation significantly reduced scleroglucan reactivity, being EPS II the most stable glucan, whilst EPSi and LSCL were the most affected ones (91-99% reduction). Single helix activation ability was significantly dependent on polysaccharide concentration. Scleroglucans from S. rolfsii ATCC 201126 (EPS I and EPS II) exhibited marked clotting activity against factor G and were more effective than commercial scleroglucan. Denaturation would lead to lower biological activity and it would be dependent on EPS concentration.
Fil: Viñarta, Silvana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Virgili Alemán, Inés María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XLVI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Chubut
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
description The ability of scleroglucan (beta-1,3-beta-1,6 glucan) conformers to activate Limulus coagulation factor G was evaluated. Studies pursued to get a further insight into the scleroglucan structure-function relationship. Lab-scale produced (EPS I, EPS II, EPSi) and commercial (LSCL) scleroglucans were tested on their ability to activate factor G of the Limulus coagulation cascade (Glucatell Test, Pyrolab). Native triplex conformation was obtained by dissolving scleroglucan (2 pg/mL) in distilled water. Thermally- (150°C, 30 min) and alkali-treated (0·2 N NaOH, 10 min) scleroglucans were also prepared. Single helices were further evaluated at 2 ng/mL and 2 µg/mL. All scleroglucans were endotoxin-free (LAL-Test, Pyrolab). Triplex conformation had stronger ability to activate factor G than single helix, with EPS I and EPS II as the most active polymers. Thermal and alkaline denaturation significantly reduced scleroglucan reactivity, being EPS II the most stable glucan, whilst EPSi and LSCL were the most affected ones (91-99% reduction). Single helix activation ability was significantly dependent on polysaccharide concentration. Scleroglucans from S. rolfsii ATCC 201126 (EPS I and EPS II) exhibited marked clotting activity against factor G and were more effective than commercial scleroglucan. Denaturation would lead to lower biological activity and it would be dependent on EPS concentration.
publishDate 2010
dc.date.none.fl_str_mv 2010
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/214198
Activation of Limulus Coagulation Factor G by Scleroglucan as a Biological Activity Measurement; XLVI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Chubut; Argentina; 2010; 98-98
0327-9545
CONICET Digital
CONICET
url http://hdl.handle.net/11336/214198
identifier_str_mv Activation of Limulus Coagulation Factor G by Scleroglucan as a Biological Activity Measurement; XLVI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Chubut; Argentina; 2010; 98-98
0327-9545
CONICET Digital
CONICET
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://saib.org.ar/archivos/biocell-34.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.071171

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