Folate producing Streptococcus thermophilus CRL808 decreased sympthoms of neurological damages in a mouse parkinson's disease model and exerted neuroprotector effect in vitro

Autores
Pérez Visñuk, Daiana Emilce; Savoy, Graciela; Leblanc, Jean Guy Joseph; de Moreno, Maria Alejandra
Año de publicación
2018
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Parkinson's disease (PD) results from the dysfunction and degeneration of dopaminergic neurons within the substantia nigra pars compacta, and is characterized by persistent tremors, bradykinesia, rigidity and instability in posture. At the cellular level, the pathogenic process involves high levels of oxidative stress, mitochondrial dysfunction and apoptosis. Many studies showed that dietary factors, including B-Group vitamins, may be involved in the etiology of PD. It has been shown that patients with PD present folate deficiencies with increases in homocysteinemia. The aim of this study was to evaluate the effect of Streptococcus (S.) thermophilus CRL808, a folate-producing strain, in an animal model of PD and in vitro using neuron cell cultures. For the in vivo study, C56BL/6 mice were injected subcutaneously with the neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), using 5 doses of 20 mg/kg each during 4 days. Mice were also injected intraperitoneally with probenecid (250 mg/kg) to decrease the renal excretion of MPTP and achieve the chronicity. S. thermophilus CRL808 was orally administered (1x108 CFU/ml) starting 7 days before injection with MPTP until the end of the experiment. Each mouse received 100µl of the bacterial suspension daily. Control animals injected or not with MPTP received 100µl of physiological solution. Each mouse was subjected to behavior tests (vertical and horizontal bar, and the nasal bridge adhesive elimination). For the in vitro model, the mouse Neuro-2a (N2a) neuroblastoma cells were cultured in the presence and absence of folic acid. Oxidative stress was induced with MPP+ (toxic metabolite from MPTP). The intracellular extract from S. thermophilus CRL808 was added to the cell cultures and the effect was compared with commercial folic acid. Cell viability and proliferation were evaluated using the MTT assay. Results obtained in the in vivo model of PD showed that mice that received S. thermophilus CRL808 presented milder symptoms of the pathology compared to the PD control group. This effect was observed in the three evaluated behavior tests, in which on average, the time used to complete each test was reduced by half when compared with the PD control, with results close to those of healthy control animals. The in vitro assay showed that the exposure to MPP+ significantly increased the death of N2a cells. In contrast, when vitamin B9 or the intracellular extract of S. thermophilus CRL808 was added to the culture medium, this effect was reversed or significantly decreased. The results obtained demonstrate the neuroprotective properties of S. thermophilus CRL808 against the toxicity of MPTP and MPP+. This bacterial strain has an important potential for future studies in the Parkinson´s disease model.
Fil: Pérez Visñuk, Daiana Emilce. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Savoy, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
XIII Congreso Argentino de Microbiología General
San Luis
Argentina
Sociedad Argentina de Microbiología General
Materia
BACTERIAS LACTICAS
VITAMINAS
ENFERMEDADES NEURODEGENERATIVAS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/215762
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/215762
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Folate producing Streptococcus thermophilus CRL808 decreased sympthoms of neurological damages in a mouse parkinson's disease model and exerted neuroprotector effect in vitroPérez Visñuk, Daiana EmilceSavoy, GracielaLeblanc, Jean Guy Josephde Moreno, Maria AlejandraBACTERIAS LACTICASVITAMINASENFERMEDADES NEURODEGENERATIVAShttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Parkinson's disease (PD) results from the dysfunction and degeneration of dopaminergic neurons within the substantia nigra pars compacta, and is characterized by persistent tremors, bradykinesia, rigidity and instability in posture. At the cellular level, the pathogenic process involves high levels of oxidative stress, mitochondrial dysfunction and apoptosis. Many studies showed that dietary factors, including B-Group vitamins, may be involved in the etiology of PD. It has been shown that patients with PD present folate deficiencies with increases in homocysteinemia. The aim of this study was to evaluate the effect of Streptococcus (S.) thermophilus CRL808, a folate-producing strain, in an animal model of PD and in vitro using neuron cell cultures. For the in vivo study, C56BL/6 mice were injected subcutaneously with the neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), using 5 doses of 20 mg/kg each during 4 days. Mice were also injected intraperitoneally with probenecid (250 mg/kg) to decrease the renal excretion of MPTP and achieve the chronicity. S. thermophilus CRL808 was orally administered (1x108 CFU/ml) starting 7 days before injection with MPTP until the end of the experiment. Each mouse received 100µl of the bacterial suspension daily. Control animals injected or not with MPTP received 100µl of physiological solution. Each mouse was subjected to behavior tests (vertical and horizontal bar, and the nasal bridge adhesive elimination). For the in vitro model, the mouse Neuro-2a (N2a) neuroblastoma cells were cultured in the presence and absence of folic acid. Oxidative stress was induced with MPP+ (toxic metabolite from MPTP). The intracellular extract from S. thermophilus CRL808 was added to the cell cultures and the effect was compared with commercial folic acid. Cell viability and proliferation were evaluated using the MTT assay. Results obtained in the in vivo model of PD showed that mice that received S. thermophilus CRL808 presented milder symptoms of the pathology compared to the PD control group. This effect was observed in the three evaluated behavior tests, in which on average, the time used to complete each test was reduced by half when compared with the PD control, with results close to those of healthy control animals. The in vitro assay showed that the exposure to MPP+ significantly increased the death of N2a cells. In contrast, when vitamin B9 or the intracellular extract of S. thermophilus CRL808 was added to the culture medium, this effect was reversed or significantly decreased. The results obtained demonstrate the neuroprotective properties of S. thermophilus CRL808 against the toxicity of MPTP and MPP+. This bacterial strain has an important potential for future studies in the Parkinson´s disease model.Fil: Pérez Visñuk, Daiana Emilce. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Savoy, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaXIII Congreso Argentino de Microbiología GeneralSan LuisArgentinaSociedad Argentina de Microbiología GeneralAsociación Civil de Microbiología General2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/215762Folate producing Streptococcus thermophilus CRL808 decreased sympthoms of neurological damages in a mouse parkinson's disease model and exerted neuroprotector effect in vitro; XIII Congreso Argentino de Microbiología General; San Luis; Argentina; 2018; 40-40CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://samige.org.ar//wp-content/uploads/2022/10/Libro-samige-2018-v3.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-04-23T14:52:02Zoai:ri.conicet.gov.ar:11336/215762instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-04-23 14:52:03.069CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Folate producing Streptococcus thermophilus CRL808 decreased sympthoms of neurological damages in a mouse parkinson's disease model and exerted neuroprotector effect in vitro
title Folate producing Streptococcus thermophilus CRL808 decreased sympthoms of neurological damages in a mouse parkinson's disease model and exerted neuroprotector effect in vitro
spellingShingle Folate producing Streptococcus thermophilus CRL808 decreased sympthoms of neurological damages in a mouse parkinson's disease model and exerted neuroprotector effect in vitro
Pérez Visñuk, Daiana Emilce
BACTERIAS LACTICAS
VITAMINAS
ENFERMEDADES NEURODEGENERATIVAS
title_short Folate producing Streptococcus thermophilus CRL808 decreased sympthoms of neurological damages in a mouse parkinson's disease model and exerted neuroprotector effect in vitro
title_full Folate producing Streptococcus thermophilus CRL808 decreased sympthoms of neurological damages in a mouse parkinson's disease model and exerted neuroprotector effect in vitro
title_fullStr Folate producing Streptococcus thermophilus CRL808 decreased sympthoms of neurological damages in a mouse parkinson's disease model and exerted neuroprotector effect in vitro
title_full_unstemmed Folate producing Streptococcus thermophilus CRL808 decreased sympthoms of neurological damages in a mouse parkinson's disease model and exerted neuroprotector effect in vitro
title_sort Folate producing Streptococcus thermophilus CRL808 decreased sympthoms of neurological damages in a mouse parkinson's disease model and exerted neuroprotector effect in vitro
dc.creator.none.fl_str_mv Pérez Visñuk, Daiana Emilce
Savoy, Graciela
Leblanc, Jean Guy Joseph
de Moreno, Maria Alejandra
author Pérez Visñuk, Daiana Emilce
author_facet Pérez Visñuk, Daiana Emilce
Savoy, Graciela
Leblanc, Jean Guy Joseph
de Moreno, Maria Alejandra
author_role author
author2 Savoy, Graciela
Leblanc, Jean Guy Joseph
de Moreno, Maria Alejandra
author2_role author
author
author
dc.subject.none.fl_str_mv BACTERIAS LACTICAS
VITAMINAS
ENFERMEDADES NEURODEGENERATIVAS
topic BACTERIAS LACTICAS
VITAMINAS
ENFERMEDADES NEURODEGENERATIVAS
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Parkinson's disease (PD) results from the dysfunction and degeneration of dopaminergic neurons within the substantia nigra pars compacta, and is characterized by persistent tremors, bradykinesia, rigidity and instability in posture. At the cellular level, the pathogenic process involves high levels of oxidative stress, mitochondrial dysfunction and apoptosis. Many studies showed that dietary factors, including B-Group vitamins, may be involved in the etiology of PD. It has been shown that patients with PD present folate deficiencies with increases in homocysteinemia. The aim of this study was to evaluate the effect of Streptococcus (S.) thermophilus CRL808, a folate-producing strain, in an animal model of PD and in vitro using neuron cell cultures. For the in vivo study, C56BL/6 mice were injected subcutaneously with the neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), using 5 doses of 20 mg/kg each during 4 days. Mice were also injected intraperitoneally with probenecid (250 mg/kg) to decrease the renal excretion of MPTP and achieve the chronicity. S. thermophilus CRL808 was orally administered (1x108 CFU/ml) starting 7 days before injection with MPTP until the end of the experiment. Each mouse received 100µl of the bacterial suspension daily. Control animals injected or not with MPTP received 100µl of physiological solution. Each mouse was subjected to behavior tests (vertical and horizontal bar, and the nasal bridge adhesive elimination). For the in vitro model, the mouse Neuro-2a (N2a) neuroblastoma cells were cultured in the presence and absence of folic acid. Oxidative stress was induced with MPP+ (toxic metabolite from MPTP). The intracellular extract from S. thermophilus CRL808 was added to the cell cultures and the effect was compared with commercial folic acid. Cell viability and proliferation were evaluated using the MTT assay. Results obtained in the in vivo model of PD showed that mice that received S. thermophilus CRL808 presented milder symptoms of the pathology compared to the PD control group. This effect was observed in the three evaluated behavior tests, in which on average, the time used to complete each test was reduced by half when compared with the PD control, with results close to those of healthy control animals. The in vitro assay showed that the exposure to MPP+ significantly increased the death of N2a cells. In contrast, when vitamin B9 or the intracellular extract of S. thermophilus CRL808 was added to the culture medium, this effect was reversed or significantly decreased. The results obtained demonstrate the neuroprotective properties of S. thermophilus CRL808 against the toxicity of MPTP and MPP+. This bacterial strain has an important potential for future studies in the Parkinson´s disease model.
Fil: Pérez Visñuk, Daiana Emilce. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Savoy, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
XIII Congreso Argentino de Microbiología General
San Luis
Argentina
Sociedad Argentina de Microbiología General
description Parkinson's disease (PD) results from the dysfunction and degeneration of dopaminergic neurons within the substantia nigra pars compacta, and is characterized by persistent tremors, bradykinesia, rigidity and instability in posture. At the cellular level, the pathogenic process involves high levels of oxidative stress, mitochondrial dysfunction and apoptosis. Many studies showed that dietary factors, including B-Group vitamins, may be involved in the etiology of PD. It has been shown that patients with PD present folate deficiencies with increases in homocysteinemia. The aim of this study was to evaluate the effect of Streptococcus (S.) thermophilus CRL808, a folate-producing strain, in an animal model of PD and in vitro using neuron cell cultures. For the in vivo study, C56BL/6 mice were injected subcutaneously with the neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), using 5 doses of 20 mg/kg each during 4 days. Mice were also injected intraperitoneally with probenecid (250 mg/kg) to decrease the renal excretion of MPTP and achieve the chronicity. S. thermophilus CRL808 was orally administered (1x108 CFU/ml) starting 7 days before injection with MPTP until the end of the experiment. Each mouse received 100µl of the bacterial suspension daily. Control animals injected or not with MPTP received 100µl of physiological solution. Each mouse was subjected to behavior tests (vertical and horizontal bar, and the nasal bridge adhesive elimination). For the in vitro model, the mouse Neuro-2a (N2a) neuroblastoma cells were cultured in the presence and absence of folic acid. Oxidative stress was induced with MPP+ (toxic metabolite from MPTP). The intracellular extract from S. thermophilus CRL808 was added to the cell cultures and the effect was compared with commercial folic acid. Cell viability and proliferation were evaluated using the MTT assay. Results obtained in the in vivo model of PD showed that mice that received S. thermophilus CRL808 presented milder symptoms of the pathology compared to the PD control group. This effect was observed in the three evaluated behavior tests, in which on average, the time used to complete each test was reduced by half when compared with the PD control, with results close to those of healthy control animals. The in vitro assay showed that the exposure to MPP+ significantly increased the death of N2a cells. In contrast, when vitamin B9 or the intracellular extract of S. thermophilus CRL808 was added to the culture medium, this effect was reversed or significantly decreased. The results obtained demonstrate the neuroprotective properties of S. thermophilus CRL808 against the toxicity of MPTP and MPP+. This bacterial strain has an important potential for future studies in the Parkinson´s disease model.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/215762
Folate producing Streptococcus thermophilus CRL808 decreased sympthoms of neurological damages in a mouse parkinson's disease model and exerted neuroprotector effect in vitro; XIII Congreso Argentino de Microbiología General; San Luis; Argentina; 2018; 40-40
CONICET Digital
CONICET
url http://hdl.handle.net/11336/215762
identifier_str_mv Folate producing Streptococcus thermophilus CRL808 decreased sympthoms of neurological damages in a mouse parkinson's disease model and exerted neuroprotector effect in vitro; XIII Congreso Argentino de Microbiología General; San Luis; Argentina; 2018; 40-40
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://samige.org.ar//wp-content/uploads/2022/10/Libro-samige-2018-v3.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Asociación Civil de Microbiología General
publisher.none.fl_str_mv Asociación Civil de Microbiología General
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
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