Folate production and fol genes expression by the dairy starter culture Streptococcus thermophilus CRL803 in free and controlled pH batch fermentations

Autores
Laiño, Jonathan Emiliano; Juarez del Valle, Marianela; Hebert, Elvira Maria; Savoy, Graciela; Leblanc, Jean Guy Joseph
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Folate production by Streptococcus thermophilus CRL 803 was examined during batch fermentations without pH control and at constant pH (6.0 and 5.0) at different incubation temperatures. Folate production was higher at 42 °C under both constant and non-constant pH fermentation conditions, the increase being more pronounced at constant pH 6.0. Folate production by S. thermophilus CRL 803 under different fermentation conditions was cell-growth-associated. These observations were supported by analysis at the transcriptional levels of fol genes in controlled pH of 6.0 and uncontrolled pH batch culture. At pH 6.0, the highest relative expression levels of folE, folQ, folK and folP genes (involved in de novo synthesis) were observed after 6 h of incubation. This is the first study on the expression of fol mRNA and their relation with folate synthesis by S. thermophilus, which is influenced by culture conditions.
Fil: Laiño, Jonathan Emiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Juarez del Valle, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Hebert, Elvira Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Savoy, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Materia
Culture Conditions
Fol Genes Expression
Folate
Lactic Acid Bacteria
Streptococcus Thermophilus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/40079
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/40079
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Folate production and fol genes expression by the dairy starter culture Streptococcus thermophilus CRL803 in free and controlled pH batch fermentationsLaiño, Jonathan EmilianoJuarez del Valle, MarianelaHebert, Elvira MariaSavoy, GracielaLeblanc, Jean Guy JosephCulture ConditionsFol Genes ExpressionFolateLactic Acid BacteriaStreptococcus Thermophilushttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Folate production by Streptococcus thermophilus CRL 803 was examined during batch fermentations without pH control and at constant pH (6.0 and 5.0) at different incubation temperatures. Folate production was higher at 42 °C under both constant and non-constant pH fermentation conditions, the increase being more pronounced at constant pH 6.0. Folate production by S. thermophilus CRL 803 under different fermentation conditions was cell-growth-associated. These observations were supported by analysis at the transcriptional levels of fol genes in controlled pH of 6.0 and uncontrolled pH batch culture. At pH 6.0, the highest relative expression levels of folE, folQ, folK and folP genes (involved in de novo synthesis) were observed after 6 h of incubation. This is the first study on the expression of fol mRNA and their relation with folate synthesis by S. thermophilus, which is influenced by culture conditions.Fil: Laiño, Jonathan Emiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaFil: Juarez del Valle, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaFil: Hebert, Elvira Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaFil: Savoy, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaFil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaElsevier Science2017-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/40079Laiño, Jonathan Emiliano; Juarez del Valle, Marianela; Hebert, Elvira Maria; Savoy, Graciela; Leblanc, Jean Guy Joseph; Folate production and fol genes expression by the dairy starter culture Streptococcus thermophilus CRL803 in free and controlled pH batch fermentations; Elsevier Science; LWT - Food Science and Technology; 85; 11-2017; 146-1500023-6438CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2017.07.004info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0023643817304735info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:26:11Zoai:ri.conicet.gov.ar:11336/40079instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:26:11.955CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Folate production and fol genes expression by the dairy starter culture Streptococcus thermophilus CRL803 in free and controlled pH batch fermentations
title Folate production and fol genes expression by the dairy starter culture Streptococcus thermophilus CRL803 in free and controlled pH batch fermentations
spellingShingle Folate production and fol genes expression by the dairy starter culture Streptococcus thermophilus CRL803 in free and controlled pH batch fermentations
Laiño, Jonathan Emiliano
Culture Conditions
Fol Genes Expression
Folate
Lactic Acid Bacteria
Streptococcus Thermophilus
title_short Folate production and fol genes expression by the dairy starter culture Streptococcus thermophilus CRL803 in free and controlled pH batch fermentations
title_full Folate production and fol genes expression by the dairy starter culture Streptococcus thermophilus CRL803 in free and controlled pH batch fermentations
title_fullStr Folate production and fol genes expression by the dairy starter culture Streptococcus thermophilus CRL803 in free and controlled pH batch fermentations
title_full_unstemmed Folate production and fol genes expression by the dairy starter culture Streptococcus thermophilus CRL803 in free and controlled pH batch fermentations
title_sort Folate production and fol genes expression by the dairy starter culture Streptococcus thermophilus CRL803 in free and controlled pH batch fermentations
dc.creator.none.fl_str_mv Laiño, Jonathan Emiliano
Juarez del Valle, Marianela
Hebert, Elvira Maria
Savoy, Graciela
Leblanc, Jean Guy Joseph
author Laiño, Jonathan Emiliano
author_facet Laiño, Jonathan Emiliano
Juarez del Valle, Marianela
Hebert, Elvira Maria
Savoy, Graciela
Leblanc, Jean Guy Joseph
author_role author
author2 Juarez del Valle, Marianela
Hebert, Elvira Maria
Savoy, Graciela
Leblanc, Jean Guy Joseph
author2_role author
author
author
author
dc.subject.none.fl_str_mv Culture Conditions
Fol Genes Expression
Folate
Lactic Acid Bacteria
Streptococcus Thermophilus
topic Culture Conditions
Fol Genes Expression
Folate
Lactic Acid Bacteria
Streptococcus Thermophilus
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Folate production by Streptococcus thermophilus CRL 803 was examined during batch fermentations without pH control and at constant pH (6.0 and 5.0) at different incubation temperatures. Folate production was higher at 42 °C under both constant and non-constant pH fermentation conditions, the increase being more pronounced at constant pH 6.0. Folate production by S. thermophilus CRL 803 under different fermentation conditions was cell-growth-associated. These observations were supported by analysis at the transcriptional levels of fol genes in controlled pH of 6.0 and uncontrolled pH batch culture. At pH 6.0, the highest relative expression levels of folE, folQ, folK and folP genes (involved in de novo synthesis) were observed after 6 h of incubation. This is the first study on the expression of fol mRNA and their relation with folate synthesis by S. thermophilus, which is influenced by culture conditions.
Fil: Laiño, Jonathan Emiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Juarez del Valle, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Hebert, Elvira Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Savoy, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
description Folate production by Streptococcus thermophilus CRL 803 was examined during batch fermentations without pH control and at constant pH (6.0 and 5.0) at different incubation temperatures. Folate production was higher at 42 °C under both constant and non-constant pH fermentation conditions, the increase being more pronounced at constant pH 6.0. Folate production by S. thermophilus CRL 803 under different fermentation conditions was cell-growth-associated. These observations were supported by analysis at the transcriptional levels of fol genes in controlled pH of 6.0 and uncontrolled pH batch culture. At pH 6.0, the highest relative expression levels of folE, folQ, folK and folP genes (involved in de novo synthesis) were observed after 6 h of incubation. This is the first study on the expression of fol mRNA and their relation with folate synthesis by S. thermophilus, which is influenced by culture conditions.
publishDate 2017
dc.date.none.fl_str_mv 2017-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/40079
Laiño, Jonathan Emiliano; Juarez del Valle, Marianela; Hebert, Elvira Maria; Savoy, Graciela; Leblanc, Jean Guy Joseph; Folate production and fol genes expression by the dairy starter culture Streptococcus thermophilus CRL803 in free and controlled pH batch fermentations; Elsevier Science; LWT - Food Science and Technology; 85; 11-2017; 146-150
0023-6438
CONICET Digital
CONICET
url http://hdl.handle.net/11336/40079
identifier_str_mv Laiño, Jonathan Emiliano; Juarez del Valle, Marianela; Hebert, Elvira Maria; Savoy, Graciela; Leblanc, Jean Guy Joseph; Folate production and fol genes expression by the dairy starter culture Streptococcus thermophilus CRL803 in free and controlled pH batch fermentations; Elsevier Science; LWT - Food Science and Technology; 85; 11-2017; 146-150
0023-6438
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2017.07.004
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0023643817304735
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781818070630400
score 12.982451

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