Association of folate production and immune modulation by seleceted bacteria in Parkinson's disease models
- Autores
- Pérez Visñuk, Daiana Emilce; Savoy, Graciela; Leblanc, Jean Guy Joseph; de Moreno, Maria Alejandra
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Parkinson's disease (PD) is a neurodegenerative process that affect motor functions and involves an inflammatory response and B group vitamins' deficiency. Folate depletion with hyperhomocysteinemia are related with immune activation ; however, the association between folate (vitamin B9) and the immune system in PD requires further research. Aim: To evaluate the effect of folate-producing and immunomodulatory lactic acid bacteria (LAB) in PD models. Methods. Streptococcus thermophilus (St.) CRL808 (folate producer strain), and St. CRL807 (immunomodulatory strain) were evaluated individually using in vitro and in vivo PD models. N2a neuronal cells were differentiated to dopaminergic neurons with di-butyryl cyclic AMP and then exposed to the neurotoxin 1-methyl-4-phenylpyridinium (MPP+) in presence of intracellular extracts from LAB or the commercial vitamin B9. Cell viability, IL-6 production and reactive oxygen species (ROS) were determined. In vivo, LAB were administered to mice injected with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Motor capacity, tyrosine hydroxylase (TH) in brain, and cytokines in serum were evaluated. Results: Neurotoxic effect of MPP+ decreased in cells cultured with both LAB intracellular extracts. This result was related with significant (p<0.05) decrease of ROS formation and IL-6 release by the neurons Mice given LAB improved motor skills altered by MPTP and significantly (p<0.05) increased the number of TH+ neurons in the brain . The LAB effect was associated to decreased pro-inflammatory cytokines such asIL-6 and TNF-alpha and increased levels of IL-10 in the serum of the mice. Conclusions. LAB selected as folate producers and as immunomodulators have the potential to be used as adjuvants in PD, improving both vitamin deficiency and the inflammatory state associated with this pathology, which translates into less loss of dopaminergic neurons and better motor skills.
Fil: Pérez Visñuk, Daiana Emilce. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Savoy, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); LXIX Reunión anual de la Sociedad Argentina de Immunología (SAI); LIII Reunión anual de la Asociación Argentina de Farmacología Experimental (AAFE) y XI Reunión anual de la Asociación Argentina de Nanomedicinas (NANOMED-AR)
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunologia
Asociación Argentina de Farmacología Experimental
Asociación Argentina de Nanomedicinas - Materia
-
PARKINSON DISEASE
FOLATE
LACTIC ACID BACTERIA
BRAIN-GUT AXIS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/158437
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Association of folate production and immune modulation by seleceted bacteria in Parkinson's disease modelsPérez Visñuk, Daiana EmilceSavoy, GracielaLeblanc, Jean Guy Josephde Moreno, Maria AlejandraPARKINSON DISEASEFOLATELACTIC ACID BACTERIABRAIN-GUT AXIShttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Parkinson's disease (PD) is a neurodegenerative process that affect motor functions and involves an inflammatory response and B group vitamins' deficiency. Folate depletion with hyperhomocysteinemia are related with immune activation ; however, the association between folate (vitamin B9) and the immune system in PD requires further research. Aim: To evaluate the effect of folate-producing and immunomodulatory lactic acid bacteria (LAB) in PD models. Methods. Streptococcus thermophilus (St.) CRL808 (folate producer strain), and St. CRL807 (immunomodulatory strain) were evaluated individually using in vitro and in vivo PD models. N2a neuronal cells were differentiated to dopaminergic neurons with di-butyryl cyclic AMP and then exposed to the neurotoxin 1-methyl-4-phenylpyridinium (MPP+) in presence of intracellular extracts from LAB or the commercial vitamin B9. Cell viability, IL-6 production and reactive oxygen species (ROS) were determined. In vivo, LAB were administered to mice injected with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Motor capacity, tyrosine hydroxylase (TH) in brain, and cytokines in serum were evaluated. Results: Neurotoxic effect of MPP+ decreased in cells cultured with both LAB intracellular extracts. This result was related with significant (p<0.05) decrease of ROS formation and IL-6 release by the neurons Mice given LAB improved motor skills altered by MPTP and significantly (p<0.05) increased the number of TH+ neurons in the brain . The LAB effect was associated to decreased pro-inflammatory cytokines such asIL-6 and TNF-alpha and increased levels of IL-10 in the serum of the mice. Conclusions. LAB selected as folate producers and as immunomodulators have the potential to be used as adjuvants in PD, improving both vitamin deficiency and the inflammatory state associated with this pathology, which translates into less loss of dopaminergic neurons and better motor skills.Fil: Pérez Visñuk, Daiana Emilce. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Savoy, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaLXVI Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); LXIX Reunión anual de la Sociedad Argentina de Immunología (SAI); LIII Reunión anual de la Asociación Argentina de Farmacología Experimental (AAFE) y XI Reunión anual de la Asociación Argentina de Nanomedicinas (NANOMED-AR)ArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologiaAsociación Argentina de Farmacología ExperimentalAsociación Argentina de NanomedicinasFundación Revista Medicina2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/158437Association of folate production and immune modulation by seleceted bacteria in Parkinson's disease models; LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); LXIX Reunión anual de la Sociedad Argentina de Immunología (SAI); LIII Reunión anual de la Asociación Argentina de Farmacología Experimental (AAFE) y XI Reunión anual de la Asociación Argentina de Nanomedicinas (NANOMED-AR); Argentina; 2021; 193-1930025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.reunionbiociencias.com.ar/wp-content/uploads/2021/11/Revista-Medicina-2021.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:00:58Zoai:ri.conicet.gov.ar:11336/158437instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:00:58.906CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Association of folate production and immune modulation by seleceted bacteria in Parkinson's disease models |
title |
Association of folate production and immune modulation by seleceted bacteria in Parkinson's disease models |
spellingShingle |
Association of folate production and immune modulation by seleceted bacteria in Parkinson's disease models Pérez Visñuk, Daiana Emilce PARKINSON DISEASE FOLATE LACTIC ACID BACTERIA BRAIN-GUT AXIS |
title_short |
Association of folate production and immune modulation by seleceted bacteria in Parkinson's disease models |
title_full |
Association of folate production and immune modulation by seleceted bacteria in Parkinson's disease models |
title_fullStr |
Association of folate production and immune modulation by seleceted bacteria in Parkinson's disease models |
title_full_unstemmed |
Association of folate production and immune modulation by seleceted bacteria in Parkinson's disease models |
title_sort |
Association of folate production and immune modulation by seleceted bacteria in Parkinson's disease models |
dc.creator.none.fl_str_mv |
Pérez Visñuk, Daiana Emilce Savoy, Graciela Leblanc, Jean Guy Joseph de Moreno, Maria Alejandra |
author |
Pérez Visñuk, Daiana Emilce |
author_facet |
Pérez Visñuk, Daiana Emilce Savoy, Graciela Leblanc, Jean Guy Joseph de Moreno, Maria Alejandra |
author_role |
author |
author2 |
Savoy, Graciela Leblanc, Jean Guy Joseph de Moreno, Maria Alejandra |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
PARKINSON DISEASE FOLATE LACTIC ACID BACTERIA BRAIN-GUT AXIS |
topic |
PARKINSON DISEASE FOLATE LACTIC ACID BACTERIA BRAIN-GUT AXIS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.11 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
Parkinson's disease (PD) is a neurodegenerative process that affect motor functions and involves an inflammatory response and B group vitamins' deficiency. Folate depletion with hyperhomocysteinemia are related with immune activation ; however, the association between folate (vitamin B9) and the immune system in PD requires further research. Aim: To evaluate the effect of folate-producing and immunomodulatory lactic acid bacteria (LAB) in PD models. Methods. Streptococcus thermophilus (St.) CRL808 (folate producer strain), and St. CRL807 (immunomodulatory strain) were evaluated individually using in vitro and in vivo PD models. N2a neuronal cells were differentiated to dopaminergic neurons with di-butyryl cyclic AMP and then exposed to the neurotoxin 1-methyl-4-phenylpyridinium (MPP+) in presence of intracellular extracts from LAB or the commercial vitamin B9. Cell viability, IL-6 production and reactive oxygen species (ROS) were determined. In vivo, LAB were administered to mice injected with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Motor capacity, tyrosine hydroxylase (TH) in brain, and cytokines in serum were evaluated. Results: Neurotoxic effect of MPP+ decreased in cells cultured with both LAB intracellular extracts. This result was related with significant (p<0.05) decrease of ROS formation and IL-6 release by the neurons Mice given LAB improved motor skills altered by MPTP and significantly (p<0.05) increased the number of TH+ neurons in the brain . The LAB effect was associated to decreased pro-inflammatory cytokines such asIL-6 and TNF-alpha and increased levels of IL-10 in the serum of the mice. Conclusions. LAB selected as folate producers and as immunomodulators have the potential to be used as adjuvants in PD, improving both vitamin deficiency and the inflammatory state associated with this pathology, which translates into less loss of dopaminergic neurons and better motor skills. Fil: Pérez Visñuk, Daiana Emilce. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Savoy, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); LXIX Reunión anual de la Sociedad Argentina de Immunología (SAI); LIII Reunión anual de la Asociación Argentina de Farmacología Experimental (AAFE) y XI Reunión anual de la Asociación Argentina de Nanomedicinas (NANOMED-AR) Argentina Sociedad Argentina de Investigación Clínica Sociedad Argentina de Inmunologia Asociación Argentina de Farmacología Experimental Asociación Argentina de Nanomedicinas |
description |
Parkinson's disease (PD) is a neurodegenerative process that affect motor functions and involves an inflammatory response and B group vitamins' deficiency. Folate depletion with hyperhomocysteinemia are related with immune activation ; however, the association between folate (vitamin B9) and the immune system in PD requires further research. Aim: To evaluate the effect of folate-producing and immunomodulatory lactic acid bacteria (LAB) in PD models. Methods. Streptococcus thermophilus (St.) CRL808 (folate producer strain), and St. CRL807 (immunomodulatory strain) were evaluated individually using in vitro and in vivo PD models. N2a neuronal cells were differentiated to dopaminergic neurons with di-butyryl cyclic AMP and then exposed to the neurotoxin 1-methyl-4-phenylpyridinium (MPP+) in presence of intracellular extracts from LAB or the commercial vitamin B9. Cell viability, IL-6 production and reactive oxygen species (ROS) were determined. In vivo, LAB were administered to mice injected with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Motor capacity, tyrosine hydroxylase (TH) in brain, and cytokines in serum were evaluated. Results: Neurotoxic effect of MPP+ decreased in cells cultured with both LAB intracellular extracts. This result was related with significant (p<0.05) decrease of ROS formation and IL-6 release by the neurons Mice given LAB improved motor skills altered by MPTP and significantly (p<0.05) increased the number of TH+ neurons in the brain . The LAB effect was associated to decreased pro-inflammatory cytokines such asIL-6 and TNF-alpha and increased levels of IL-10 in the serum of the mice. Conclusions. LAB selected as folate producers and as immunomodulators have the potential to be used as adjuvants in PD, improving both vitamin deficiency and the inflammatory state associated with this pathology, which translates into less loss of dopaminergic neurons and better motor skills. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Reunión Journal http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/158437 Association of folate production and immune modulation by seleceted bacteria in Parkinson's disease models; LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); LXIX Reunión anual de la Sociedad Argentina de Immunología (SAI); LIII Reunión anual de la Asociación Argentina de Farmacología Experimental (AAFE) y XI Reunión anual de la Asociación Argentina de Nanomedicinas (NANOMED-AR); Argentina; 2021; 193-193 0025-7680 1669-9106 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/158437 |
identifier_str_mv |
Association of folate production and immune modulation by seleceted bacteria in Parkinson's disease models; LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); LXIX Reunión anual de la Sociedad Argentina de Immunología (SAI); LIII Reunión anual de la Asociación Argentina de Farmacología Experimental (AAFE) y XI Reunión anual de la Asociación Argentina de Nanomedicinas (NANOMED-AR); Argentina; 2021; 193-193 0025-7680 1669-9106 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
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info:eu-repo/semantics/altIdentifier/url/https://www.reunionbiociencias.com.ar/wp-content/uploads/2021/11/Revista-Medicina-2021.pdf |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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