Association of folate production and immune modulation by seleceted bacteria in Parkinson's disease models

Autores
Pérez Visñuk, Daiana Emilce; Savoy, Graciela; Leblanc, Jean Guy Joseph; de Moreno, Maria Alejandra
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Parkinson's disease (PD) is a neurodegenerative process that affect motor functions and involves an inflammatory response and B group vitamins' deficiency. Folate depletion with hyperhomocysteinemia are related with immune activation ; however, the association between folate (vitamin B9) and the immune system in PD requires further research. Aim: To evaluate the effect of folate-producing and immunomodulatory lactic acid bacteria (LAB) in PD models. Methods. Streptococcus thermophilus (St.) CRL808 (folate producer strain), and St. CRL807 (immunomodulatory strain) were evaluated individually using in vitro and in vivo PD models. N2a neuronal cells were differentiated to dopaminergic neurons with di-butyryl cyclic AMP and then exposed to the neurotoxin 1-methyl-4-phenylpyridinium (MPP+) in presence of intracellular extracts from LAB or the commercial vitamin B9. Cell viability, IL-6 production and reactive oxygen species (ROS) were determined. In vivo, LAB were administered to mice injected with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Motor capacity, tyrosine hydroxylase (TH) in brain, and cytokines in serum were evaluated. Results: Neurotoxic effect of MPP+ decreased in cells cultured with both LAB intracellular extracts. This result was related with significant (p<0.05) decrease of ROS formation and IL-6 release by the neurons Mice given LAB improved motor skills altered by MPTP and significantly (p<0.05) increased the number of TH+ neurons in the brain . The LAB effect was associated to decreased pro-inflammatory cytokines such asIL-6 and TNF-alpha and increased levels of IL-10 in the serum of the mice. Conclusions. LAB selected as folate producers and as immunomodulators have the potential to be used as adjuvants in PD, improving both vitamin deficiency and the inflammatory state associated with this pathology, which translates into less loss of dopaminergic neurons and better motor skills.
Fil: Pérez Visñuk, Daiana Emilce. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Savoy, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); LXIX Reunión anual de la Sociedad Argentina de Immunología (SAI); LIII Reunión anual de la Asociación Argentina de Farmacología Experimental (AAFE) y XI Reunión anual de la Asociación Argentina de Nanomedicinas (NANOMED-AR)
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunologia
Asociación Argentina de Farmacología Experimental
Asociación Argentina de Nanomedicinas
Materia
PARKINSON DISEASE
FOLATE
LACTIC ACID BACTERIA
BRAIN-GUT AXIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/158437

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oai_identifier_str oai:ri.conicet.gov.ar:11336/158437
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Association of folate production and immune modulation by seleceted bacteria in Parkinson's disease modelsPérez Visñuk, Daiana EmilceSavoy, GracielaLeblanc, Jean Guy Josephde Moreno, Maria AlejandraPARKINSON DISEASEFOLATELACTIC ACID BACTERIABRAIN-GUT AXIShttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Parkinson's disease (PD) is a neurodegenerative process that affect motor functions and involves an inflammatory response and B group vitamins' deficiency. Folate depletion with hyperhomocysteinemia are related with immune activation ; however, the association between folate (vitamin B9) and the immune system in PD requires further research. Aim: To evaluate the effect of folate-producing and immunomodulatory lactic acid bacteria (LAB) in PD models. Methods. Streptococcus thermophilus (St.) CRL808 (folate producer strain), and St. CRL807 (immunomodulatory strain) were evaluated individually using in vitro and in vivo PD models. N2a neuronal cells were differentiated to dopaminergic neurons with di-butyryl cyclic AMP and then exposed to the neurotoxin 1-methyl-4-phenylpyridinium (MPP+) in presence of intracellular extracts from LAB or the commercial vitamin B9. Cell viability, IL-6 production and reactive oxygen species (ROS) were determined. In vivo, LAB were administered to mice injected with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Motor capacity, tyrosine hydroxylase (TH) in brain, and cytokines in serum were evaluated. Results: Neurotoxic effect of MPP+ decreased in cells cultured with both LAB intracellular extracts. This result was related with significant (p<0.05) decrease of ROS formation and IL-6 release by the neurons Mice given LAB improved motor skills altered by MPTP and significantly (p<0.05) increased the number of TH+ neurons in the brain . The LAB effect was associated to decreased pro-inflammatory cytokines such asIL-6 and TNF-alpha and increased levels of IL-10 in the serum of the mice. Conclusions. LAB selected as folate producers and as immunomodulators have the potential to be used as adjuvants in PD, improving both vitamin deficiency and the inflammatory state associated with this pathology, which translates into less loss of dopaminergic neurons and better motor skills.Fil: Pérez Visñuk, Daiana Emilce. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Savoy, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaLXVI Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); LXIX Reunión anual de la Sociedad Argentina de Immunología (SAI); LIII Reunión anual de la Asociación Argentina de Farmacología Experimental (AAFE) y XI Reunión anual de la Asociación Argentina de Nanomedicinas (NANOMED-AR)ArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologiaAsociación Argentina de Farmacología ExperimentalAsociación Argentina de NanomedicinasFundación Revista Medicina2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/158437Association of folate production and immune modulation by seleceted bacteria in Parkinson's disease models; LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); LXIX Reunión anual de la Sociedad Argentina de Immunología (SAI); LIII Reunión anual de la Asociación Argentina de Farmacología Experimental (AAFE) y XI Reunión anual de la Asociación Argentina de Nanomedicinas (NANOMED-AR); Argentina; 2021; 193-1930025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.reunionbiociencias.com.ar/wp-content/uploads/2021/11/Revista-Medicina-2021.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:00:58Zoai:ri.conicet.gov.ar:11336/158437instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:00:58.906CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Association of folate production and immune modulation by seleceted bacteria in Parkinson's disease models
title Association of folate production and immune modulation by seleceted bacteria in Parkinson's disease models
spellingShingle Association of folate production and immune modulation by seleceted bacteria in Parkinson's disease models
Pérez Visñuk, Daiana Emilce
PARKINSON DISEASE
FOLATE
LACTIC ACID BACTERIA
BRAIN-GUT AXIS
title_short Association of folate production and immune modulation by seleceted bacteria in Parkinson's disease models
title_full Association of folate production and immune modulation by seleceted bacteria in Parkinson's disease models
title_fullStr Association of folate production and immune modulation by seleceted bacteria in Parkinson's disease models
title_full_unstemmed Association of folate production and immune modulation by seleceted bacteria in Parkinson's disease models
title_sort Association of folate production and immune modulation by seleceted bacteria in Parkinson's disease models
dc.creator.none.fl_str_mv Pérez Visñuk, Daiana Emilce
Savoy, Graciela
Leblanc, Jean Guy Joseph
de Moreno, Maria Alejandra
author Pérez Visñuk, Daiana Emilce
author_facet Pérez Visñuk, Daiana Emilce
Savoy, Graciela
Leblanc, Jean Guy Joseph
de Moreno, Maria Alejandra
author_role author
author2 Savoy, Graciela
Leblanc, Jean Guy Joseph
de Moreno, Maria Alejandra
author2_role author
author
author
dc.subject.none.fl_str_mv PARKINSON DISEASE
FOLATE
LACTIC ACID BACTERIA
BRAIN-GUT AXIS
topic PARKINSON DISEASE
FOLATE
LACTIC ACID BACTERIA
BRAIN-GUT AXIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Parkinson's disease (PD) is a neurodegenerative process that affect motor functions and involves an inflammatory response and B group vitamins' deficiency. Folate depletion with hyperhomocysteinemia are related with immune activation ; however, the association between folate (vitamin B9) and the immune system in PD requires further research. Aim: To evaluate the effect of folate-producing and immunomodulatory lactic acid bacteria (LAB) in PD models. Methods. Streptococcus thermophilus (St.) CRL808 (folate producer strain), and St. CRL807 (immunomodulatory strain) were evaluated individually using in vitro and in vivo PD models. N2a neuronal cells were differentiated to dopaminergic neurons with di-butyryl cyclic AMP and then exposed to the neurotoxin 1-methyl-4-phenylpyridinium (MPP+) in presence of intracellular extracts from LAB or the commercial vitamin B9. Cell viability, IL-6 production and reactive oxygen species (ROS) were determined. In vivo, LAB were administered to mice injected with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Motor capacity, tyrosine hydroxylase (TH) in brain, and cytokines in serum were evaluated. Results: Neurotoxic effect of MPP+ decreased in cells cultured with both LAB intracellular extracts. This result was related with significant (p<0.05) decrease of ROS formation and IL-6 release by the neurons Mice given LAB improved motor skills altered by MPTP and significantly (p<0.05) increased the number of TH+ neurons in the brain . The LAB effect was associated to decreased pro-inflammatory cytokines such asIL-6 and TNF-alpha and increased levels of IL-10 in the serum of the mice. Conclusions. LAB selected as folate producers and as immunomodulators have the potential to be used as adjuvants in PD, improving both vitamin deficiency and the inflammatory state associated with this pathology, which translates into less loss of dopaminergic neurons and better motor skills.
Fil: Pérez Visñuk, Daiana Emilce. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Savoy, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); LXIX Reunión anual de la Sociedad Argentina de Immunología (SAI); LIII Reunión anual de la Asociación Argentina de Farmacología Experimental (AAFE) y XI Reunión anual de la Asociación Argentina de Nanomedicinas (NANOMED-AR)
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunologia
Asociación Argentina de Farmacología Experimental
Asociación Argentina de Nanomedicinas
description Parkinson's disease (PD) is a neurodegenerative process that affect motor functions and involves an inflammatory response and B group vitamins' deficiency. Folate depletion with hyperhomocysteinemia are related with immune activation ; however, the association between folate (vitamin B9) and the immune system in PD requires further research. Aim: To evaluate the effect of folate-producing and immunomodulatory lactic acid bacteria (LAB) in PD models. Methods. Streptococcus thermophilus (St.) CRL808 (folate producer strain), and St. CRL807 (immunomodulatory strain) were evaluated individually using in vitro and in vivo PD models. N2a neuronal cells were differentiated to dopaminergic neurons with di-butyryl cyclic AMP and then exposed to the neurotoxin 1-methyl-4-phenylpyridinium (MPP+) in presence of intracellular extracts from LAB or the commercial vitamin B9. Cell viability, IL-6 production and reactive oxygen species (ROS) were determined. In vivo, LAB were administered to mice injected with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Motor capacity, tyrosine hydroxylase (TH) in brain, and cytokines in serum were evaluated. Results: Neurotoxic effect of MPP+ decreased in cells cultured with both LAB intracellular extracts. This result was related with significant (p<0.05) decrease of ROS formation and IL-6 release by the neurons Mice given LAB improved motor skills altered by MPTP and significantly (p<0.05) increased the number of TH+ neurons in the brain . The LAB effect was associated to decreased pro-inflammatory cytokines such asIL-6 and TNF-alpha and increased levels of IL-10 in the serum of the mice. Conclusions. LAB selected as folate producers and as immunomodulators have the potential to be used as adjuvants in PD, improving both vitamin deficiency and the inflammatory state associated with this pathology, which translates into less loss of dopaminergic neurons and better motor skills.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
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Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/158437
Association of folate production and immune modulation by seleceted bacteria in Parkinson's disease models; LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); LXIX Reunión anual de la Sociedad Argentina de Immunología (SAI); LIII Reunión anual de la Asociación Argentina de Farmacología Experimental (AAFE) y XI Reunión anual de la Asociación Argentina de Nanomedicinas (NANOMED-AR); Argentina; 2021; 193-193
0025-7680
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/158437
identifier_str_mv Association of folate production and immune modulation by seleceted bacteria in Parkinson's disease models; LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); LXIX Reunión anual de la Sociedad Argentina de Immunología (SAI); LIII Reunión anual de la Asociación Argentina de Farmacología Experimental (AAFE) y XI Reunión anual de la Asociación Argentina de Nanomedicinas (NANOMED-AR); Argentina; 2021; 193-193
0025-7680
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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