First detection of V410L kdr mutation in Aedes aegypti populations of Argentina supported by toxicological evidence

Autores
Gonzalez, Paula Valeria; Loureiro, Aline C.; Gómez Bravo, Andrea; Castillo, Paola Marcela; Espinosa, Manuel; Gil, José Fernando; Martins, Ademir J.; Harburguer, Laura Vanesa
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aedes aegypti (L.) is the main vector of dengue, yellow fever, Zika, and chikungunya viruses in many parts of the world, impacting millions of people worldwide each year. Insecticide-based interventions have been effective in controlling Aedes mosquito populations for several years, but in recent times, resistance to these compounds has developed, posing a global threat to the control of this mosquito. Ovitraps were used to collect A. aegypti eggs in the cities of Tartagal and San Ramón de la Nueva Orán (Salta), Puerto Iguazú (Misiones), and Clorinda (Formosa). World Health Organization (WHO)-impregnated papers with the discriminating concentration (DC) of permethrin, 5X, 10X and pirimiphos methyl were used for the toxicological bioassays. We also genotyped each sample for the three kdr single nucleotide polymorphisms (SNP): V410L, V1016I, and F1534C in individual TaqMan quantitative PCR (qPCR) reactions. All investigated A. aegypti populations were highly resistant to permethrin, as the mortality percentage with the permethrin 10×DC remained below 98%. However, all populations were 100% susceptible to pirimiphos-methyl. Kdr genotyping demonstrated the presence of the V410L mutation for the first time in Argentina in all the populations studied. A prevalence of the triple mutant genotype (LL + II + CC) was observed in the northeastern cities of Clorinda (83.3%) and Puerto Iguazú (55.6%). This study demonstrates for the first time the presence and intensity of resistance to permethrin in different populations from Argentina, and correlates the observed phenotype with the presence of kdr mutations (genotype).
Fil: Gonzalez, Paula Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; Argentina
Fil: Loureiro, Aline C.. Instituto Oswaldo Cruz ; Fundacion Oswaldo Cruz ; Ministerio de Salud de Brasil;
Fil: Gómez Bravo, Andrea. Fundación Mundo Sano; Argentina
Fil: Castillo, Paola Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones en Energía no Convencional. Universidad Nacional de Salta. Facultad de Ciencias Exactas. Departamento de Física. Instituto de Investigaciones en Energía no Convencional; Argentina
Fil: Espinosa, Manuel. Fundación Mundo Sano; Argentina
Fil: Gil, José Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones en Energía no Convencional. Universidad Nacional de Salta. Facultad de Ciencias Exactas. Departamento de Física. Instituto de Investigaciones en Energía no Convencional; Argentina
Fil: Martins, Ademir J.. Instituto Oswaldo Cruz ; Fundacion Oswaldo Cruz ; Ministerio de Salud de Brasil;
Fil: Harburguer, Laura Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; Argentina
Materia
Aedes aegypti
V410L kdr mutation
Toxicologic
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/257463
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/257463
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling First detection of V410L kdr mutation in Aedes aegypti populations of Argentina supported by toxicological evidenceGonzalez, Paula ValeriaLoureiro, Aline C.Gómez Bravo, AndreaCastillo, Paola MarcelaEspinosa, ManuelGil, José FernandoMartins, Ademir J.Harburguer, Laura VanesaAedes aegyptiV410L kdr mutationToxicologichttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Aedes aegypti (L.) is the main vector of dengue, yellow fever, Zika, and chikungunya viruses in many parts of the world, impacting millions of people worldwide each year. Insecticide-based interventions have been effective in controlling Aedes mosquito populations for several years, but in recent times, resistance to these compounds has developed, posing a global threat to the control of this mosquito. Ovitraps were used to collect A. aegypti eggs in the cities of Tartagal and San Ramón de la Nueva Orán (Salta), Puerto Iguazú (Misiones), and Clorinda (Formosa). World Health Organization (WHO)-impregnated papers with the discriminating concentration (DC) of permethrin, 5X, 10X and pirimiphos methyl were used for the toxicological bioassays. We also genotyped each sample for the three kdr single nucleotide polymorphisms (SNP): V410L, V1016I, and F1534C in individual TaqMan quantitative PCR (qPCR) reactions. All investigated A. aegypti populations were highly resistant to permethrin, as the mortality percentage with the permethrin 10×DC remained below 98%. However, all populations were 100% susceptible to pirimiphos-methyl. Kdr genotyping demonstrated the presence of the V410L mutation for the first time in Argentina in all the populations studied. A prevalence of the triple mutant genotype (LL + II + CC) was observed in the northeastern cities of Clorinda (83.3%) and Puerto Iguazú (55.6%). This study demonstrates for the first time the presence and intensity of resistance to permethrin in different populations from Argentina, and correlates the observed phenotype with the presence of kdr mutations (genotype).Fil: Gonzalez, Paula Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; ArgentinaFil: Loureiro, Aline C.. Instituto Oswaldo Cruz ; Fundacion Oswaldo Cruz ; Ministerio de Salud de Brasil;Fil: Gómez Bravo, Andrea. Fundación Mundo Sano; ArgentinaFil: Castillo, Paola Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones en Energía no Convencional. Universidad Nacional de Salta. Facultad de Ciencias Exactas. Departamento de Física. Instituto de Investigaciones en Energía no Convencional; ArgentinaFil: Espinosa, Manuel. Fundación Mundo Sano; ArgentinaFil: Gil, José Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones en Energía no Convencional. Universidad Nacional de Salta. Facultad de Ciencias Exactas. Departamento de Física. Instituto de Investigaciones en Energía no Convencional; ArgentinaFil: Martins, Ademir J.. Instituto Oswaldo Cruz ; Fundacion Oswaldo Cruz ; Ministerio de Salud de Brasil;Fil: Harburguer, Laura Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; ArgentinaBioMed Central2024-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/257463Gonzalez, Paula Valeria; Loureiro, Aline C.; Gómez Bravo, Andrea; Castillo, Paola Marcela; Espinosa, Manuel; et al.; First detection of V410L kdr mutation in Aedes aegypti populations of Argentina supported by toxicological evidence; BioMed Central; Parasites and Vectors; 17; 1; 8-2024; 1-101756-3305CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-024-06405-3info:eu-repo/semantics/altIdentifier/doi/10.1186/s13071-024-06405-3info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-29T12:43:43Zoai:ri.conicet.gov.ar:11336/257463instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-29 12:43:43.61CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv First detection of V410L kdr mutation in Aedes aegypti populations of Argentina supported by toxicological evidence
title First detection of V410L kdr mutation in Aedes aegypti populations of Argentina supported by toxicological evidence
spellingShingle First detection of V410L kdr mutation in Aedes aegypti populations of Argentina supported by toxicological evidence
Gonzalez, Paula Valeria
Aedes aegypti
V410L kdr mutation
Toxicologic
title_short First detection of V410L kdr mutation in Aedes aegypti populations of Argentina supported by toxicological evidence
title_full First detection of V410L kdr mutation in Aedes aegypti populations of Argentina supported by toxicological evidence
title_fullStr First detection of V410L kdr mutation in Aedes aegypti populations of Argentina supported by toxicological evidence
title_full_unstemmed First detection of V410L kdr mutation in Aedes aegypti populations of Argentina supported by toxicological evidence
title_sort First detection of V410L kdr mutation in Aedes aegypti populations of Argentina supported by toxicological evidence
dc.creator.none.fl_str_mv Gonzalez, Paula Valeria
Loureiro, Aline C.
Gómez Bravo, Andrea
Castillo, Paola Marcela
Espinosa, Manuel
Gil, José Fernando
Martins, Ademir J.
Harburguer, Laura Vanesa
author Gonzalez, Paula Valeria
author_facet Gonzalez, Paula Valeria
Loureiro, Aline C.
Gómez Bravo, Andrea
Castillo, Paola Marcela
Espinosa, Manuel
Gil, José Fernando
Martins, Ademir J.
Harburguer, Laura Vanesa
author_role author
author2 Loureiro, Aline C.
Gómez Bravo, Andrea
Castillo, Paola Marcela
Espinosa, Manuel
Gil, José Fernando
Martins, Ademir J.
Harburguer, Laura Vanesa
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Aedes aegypti
V410L kdr mutation
Toxicologic
topic Aedes aegypti
V410L kdr mutation
Toxicologic
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Aedes aegypti (L.) is the main vector of dengue, yellow fever, Zika, and chikungunya viruses in many parts of the world, impacting millions of people worldwide each year. Insecticide-based interventions have been effective in controlling Aedes mosquito populations for several years, but in recent times, resistance to these compounds has developed, posing a global threat to the control of this mosquito. Ovitraps were used to collect A. aegypti eggs in the cities of Tartagal and San Ramón de la Nueva Orán (Salta), Puerto Iguazú (Misiones), and Clorinda (Formosa). World Health Organization (WHO)-impregnated papers with the discriminating concentration (DC) of permethrin, 5X, 10X and pirimiphos methyl were used for the toxicological bioassays. We also genotyped each sample for the three kdr single nucleotide polymorphisms (SNP): V410L, V1016I, and F1534C in individual TaqMan quantitative PCR (qPCR) reactions. All investigated A. aegypti populations were highly resistant to permethrin, as the mortality percentage with the permethrin 10×DC remained below 98%. However, all populations were 100% susceptible to pirimiphos-methyl. Kdr genotyping demonstrated the presence of the V410L mutation for the first time in Argentina in all the populations studied. A prevalence of the triple mutant genotype (LL + II + CC) was observed in the northeastern cities of Clorinda (83.3%) and Puerto Iguazú (55.6%). This study demonstrates for the first time the presence and intensity of resistance to permethrin in different populations from Argentina, and correlates the observed phenotype with the presence of kdr mutations (genotype).
Fil: Gonzalez, Paula Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; Argentina
Fil: Loureiro, Aline C.. Instituto Oswaldo Cruz ; Fundacion Oswaldo Cruz ; Ministerio de Salud de Brasil;
Fil: Gómez Bravo, Andrea. Fundación Mundo Sano; Argentina
Fil: Castillo, Paola Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones en Energía no Convencional. Universidad Nacional de Salta. Facultad de Ciencias Exactas. Departamento de Física. Instituto de Investigaciones en Energía no Convencional; Argentina
Fil: Espinosa, Manuel. Fundación Mundo Sano; Argentina
Fil: Gil, José Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones en Energía no Convencional. Universidad Nacional de Salta. Facultad de Ciencias Exactas. Departamento de Física. Instituto de Investigaciones en Energía no Convencional; Argentina
Fil: Martins, Ademir J.. Instituto Oswaldo Cruz ; Fundacion Oswaldo Cruz ; Ministerio de Salud de Brasil;
Fil: Harburguer, Laura Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; Argentina
description Aedes aegypti (L.) is the main vector of dengue, yellow fever, Zika, and chikungunya viruses in many parts of the world, impacting millions of people worldwide each year. Insecticide-based interventions have been effective in controlling Aedes mosquito populations for several years, but in recent times, resistance to these compounds has developed, posing a global threat to the control of this mosquito. Ovitraps were used to collect A. aegypti eggs in the cities of Tartagal and San Ramón de la Nueva Orán (Salta), Puerto Iguazú (Misiones), and Clorinda (Formosa). World Health Organization (WHO)-impregnated papers with the discriminating concentration (DC) of permethrin, 5X, 10X and pirimiphos methyl were used for the toxicological bioassays. We also genotyped each sample for the three kdr single nucleotide polymorphisms (SNP): V410L, V1016I, and F1534C in individual TaqMan quantitative PCR (qPCR) reactions. All investigated A. aegypti populations were highly resistant to permethrin, as the mortality percentage with the permethrin 10×DC remained below 98%. However, all populations were 100% susceptible to pirimiphos-methyl. Kdr genotyping demonstrated the presence of the V410L mutation for the first time in Argentina in all the populations studied. A prevalence of the triple mutant genotype (LL + II + CC) was observed in the northeastern cities of Clorinda (83.3%) and Puerto Iguazú (55.6%). This study demonstrates for the first time the presence and intensity of resistance to permethrin in different populations from Argentina, and correlates the observed phenotype with the presence of kdr mutations (genotype).
publishDate 2024
dc.date.none.fl_str_mv 2024-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/257463
Gonzalez, Paula Valeria; Loureiro, Aline C.; Gómez Bravo, Andrea; Castillo, Paola Marcela; Espinosa, Manuel; et al.; First detection of V410L kdr mutation in Aedes aegypti populations of Argentina supported by toxicological evidence; BioMed Central; Parasites and Vectors; 17; 1; 8-2024; 1-10
1756-3305
CONICET Digital
CONICET
url http://hdl.handle.net/11336/257463
identifier_str_mv Gonzalez, Paula Valeria; Loureiro, Aline C.; Gómez Bravo, Andrea; Castillo, Paola Marcela; Espinosa, Manuel; et al.; First detection of V410L kdr mutation in Aedes aegypti populations of Argentina supported by toxicological evidence; BioMed Central; Parasites and Vectors; 17; 1; 8-2024; 1-10
1756-3305
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-024-06405-3
info:eu-repo/semantics/altIdentifier/doi/10.1186/s13071-024-06405-3
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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