Comparison of different fertilisation media for an in vitro maturation-fertilisation-culture system using flow-cytometrically sorted X chromosome-bearing spermatozoa for bovine emb...

Autores
Ferré, Luis Bernardo; Bogliotti, Yanina; Chitwood, James L.; Fresno Rodríguez, Cristóbal; Ortega, Hugo Hector; Kjelland, Michael E.; ROSS, Pablo Juan
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
High demand exists among commercial cattle producers for in vitro-derived bovine embryos fertilised with female sex-sorted spermatozoa from high-value breeding stock. The aim of this study was to evaluate three fertilisation media, namely M199, synthetic oviductal fluid (SOF) and Tyrode's albumin-lactate-pyruvate (TALP), on IVF performance using female sex-sorted spermatozoa. In all, 1143, 1220 and 1041 cumulus-oocyte complexes were fertilised in M199, SOF and TALP, respectively. There were significant differences among fertilisation media (P<0.05) in cleavage rate (M199≤57%, SOF≤71% and TALP≤72%), blastocyst formation (M199≤9%, SOF≤20% and TALP≤19%), proportion of Grade 1 blastocysts (M199≤15%, SOF≤52% and TALP≤51%), proportion of Grade 3 blastocysts (M199≤58%, SOF≤21% and TALP≤20%) and hatching rates (M199≤29%, SOF≤60% and TALP≤65%). The inner cell mass (ICM) and trophectoderm (TE) cells of Day 7 blastocysts were also affected by the fertilisation medium. Embryos derived from SOF and TALP fertilisation media had higher numbers of ICM, TE and total cells than those fertilised in M199. In conclusion, fertilisation media affected cleavage rate, as well as subsequent embryo development, quality and hatching ability. SOF and TALP fertilisation media produced significantly more embryos of higher quality than M199.
Fil: Ferré, Luis Bernardo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Bogliotti, Yanina. University of California; Estados Unidos
Fil: Chitwood, James L.. University of California; Estados Unidos
Fil: Fresno Rodríguez, Cristóbal. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Catolica de Córdoba. Facultad de Ingeniería; Argentina
Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Kjelland, Michael E.. Conservation, Genetics and Biotech; Estados Unidos
Fil: ROSS, Pablo Juan. University of California; Estados Unidos
Materia
In Vitro Produced
Zona Pellucida
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/52574
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/52574
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Comparison of different fertilisation media for an in vitro maturation-fertilisation-culture system using flow-cytometrically sorted X chromosome-bearing spermatozoa for bovine embryo productionFerré, Luis BernardoBogliotti, YaninaChitwood, James L.Fresno Rodríguez, CristóbalOrtega, Hugo HectorKjelland, Michael E.ROSS, Pablo JuanIn Vitro ProducedZona Pellucidahttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4High demand exists among commercial cattle producers for in vitro-derived bovine embryos fertilised with female sex-sorted spermatozoa from high-value breeding stock. The aim of this study was to evaluate three fertilisation media, namely M199, synthetic oviductal fluid (SOF) and Tyrode's albumin-lactate-pyruvate (TALP), on IVF performance using female sex-sorted spermatozoa. In all, 1143, 1220 and 1041 cumulus-oocyte complexes were fertilised in M199, SOF and TALP, respectively. There were significant differences among fertilisation media (P<0.05) in cleavage rate (M199≤57%, SOF≤71% and TALP≤72%), blastocyst formation (M199≤9%, SOF≤20% and TALP≤19%), proportion of Grade 1 blastocysts (M199≤15%, SOF≤52% and TALP≤51%), proportion of Grade 3 blastocysts (M199≤58%, SOF≤21% and TALP≤20%) and hatching rates (M199≤29%, SOF≤60% and TALP≤65%). The inner cell mass (ICM) and trophectoderm (TE) cells of Day 7 blastocysts were also affected by the fertilisation medium. Embryos derived from SOF and TALP fertilisation media had higher numbers of ICM, TE and total cells than those fertilised in M199. In conclusion, fertilisation media affected cleavage rate, as well as subsequent embryo development, quality and hatching ability. SOF and TALP fertilisation media produced significantly more embryos of higher quality than M199.Fil: Ferré, Luis Bernardo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Bogliotti, Yanina. University of California; Estados UnidosFil: Chitwood, James L.. University of California; Estados UnidosFil: Fresno Rodríguez, Cristóbal. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Catolica de Córdoba. Facultad de Ingeniería; ArgentinaFil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Kjelland, Michael E.. Conservation, Genetics and Biotech; Estados UnidosFil: ROSS, Pablo Juan. University of California; Estados UnidosCsiro Publishing2016-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/52574Ferré, Luis Bernardo; Bogliotti, Yanina; Chitwood, James L.; Fresno Rodríguez, Cristóbal; Ortega, Hugo Hector; et al.; Comparison of different fertilisation media for an in vitro maturation-fertilisation-culture system using flow-cytometrically sorted X chromosome-bearing spermatozoa for bovine embryo production; Csiro Publishing; Reproduction Fertility and Development; 28; 11; 11-2016; 1695-17031031-3613CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1071/RD15019info:eu-repo/semantics/altIdentifier/url/http://www.publish.csiro.au/rd/RD15019info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:48:57Zoai:ri.conicet.gov.ar:11336/52574instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:48:58.173CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Comparison of different fertilisation media for an in vitro maturation-fertilisation-culture system using flow-cytometrically sorted X chromosome-bearing spermatozoa for bovine embryo production
title Comparison of different fertilisation media for an in vitro maturation-fertilisation-culture system using flow-cytometrically sorted X chromosome-bearing spermatozoa for bovine embryo production
spellingShingle Comparison of different fertilisation media for an in vitro maturation-fertilisation-culture system using flow-cytometrically sorted X chromosome-bearing spermatozoa for bovine embryo production
Ferré, Luis Bernardo
In Vitro Produced
Zona Pellucida
title_short Comparison of different fertilisation media for an in vitro maturation-fertilisation-culture system using flow-cytometrically sorted X chromosome-bearing spermatozoa for bovine embryo production
title_full Comparison of different fertilisation media for an in vitro maturation-fertilisation-culture system using flow-cytometrically sorted X chromosome-bearing spermatozoa for bovine embryo production
title_fullStr Comparison of different fertilisation media for an in vitro maturation-fertilisation-culture system using flow-cytometrically sorted X chromosome-bearing spermatozoa for bovine embryo production
title_full_unstemmed Comparison of different fertilisation media for an in vitro maturation-fertilisation-culture system using flow-cytometrically sorted X chromosome-bearing spermatozoa for bovine embryo production
title_sort Comparison of different fertilisation media for an in vitro maturation-fertilisation-culture system using flow-cytometrically sorted X chromosome-bearing spermatozoa for bovine embryo production
dc.creator.none.fl_str_mv Ferré, Luis Bernardo
Bogliotti, Yanina
Chitwood, James L.
Fresno Rodríguez, Cristóbal
Ortega, Hugo Hector
Kjelland, Michael E.
ROSS, Pablo Juan
author Ferré, Luis Bernardo
author_facet Ferré, Luis Bernardo
Bogliotti, Yanina
Chitwood, James L.
Fresno Rodríguez, Cristóbal
Ortega, Hugo Hector
Kjelland, Michael E.
ROSS, Pablo Juan
author_role author
author2 Bogliotti, Yanina
Chitwood, James L.
Fresno Rodríguez, Cristóbal
Ortega, Hugo Hector
Kjelland, Michael E.
ROSS, Pablo Juan
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv In Vitro Produced
Zona Pellucida
topic In Vitro Produced
Zona Pellucida
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv High demand exists among commercial cattle producers for in vitro-derived bovine embryos fertilised with female sex-sorted spermatozoa from high-value breeding stock. The aim of this study was to evaluate three fertilisation media, namely M199, synthetic oviductal fluid (SOF) and Tyrode's albumin-lactate-pyruvate (TALP), on IVF performance using female sex-sorted spermatozoa. In all, 1143, 1220 and 1041 cumulus-oocyte complexes were fertilised in M199, SOF and TALP, respectively. There were significant differences among fertilisation media (P<0.05) in cleavage rate (M199≤57%, SOF≤71% and TALP≤72%), blastocyst formation (M199≤9%, SOF≤20% and TALP≤19%), proportion of Grade 1 blastocysts (M199≤15%, SOF≤52% and TALP≤51%), proportion of Grade 3 blastocysts (M199≤58%, SOF≤21% and TALP≤20%) and hatching rates (M199≤29%, SOF≤60% and TALP≤65%). The inner cell mass (ICM) and trophectoderm (TE) cells of Day 7 blastocysts were also affected by the fertilisation medium. Embryos derived from SOF and TALP fertilisation media had higher numbers of ICM, TE and total cells than those fertilised in M199. In conclusion, fertilisation media affected cleavage rate, as well as subsequent embryo development, quality and hatching ability. SOF and TALP fertilisation media produced significantly more embryos of higher quality than M199.
Fil: Ferré, Luis Bernardo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Bogliotti, Yanina. University of California; Estados Unidos
Fil: Chitwood, James L.. University of California; Estados Unidos
Fil: Fresno Rodríguez, Cristóbal. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Catolica de Córdoba. Facultad de Ingeniería; Argentina
Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Kjelland, Michael E.. Conservation, Genetics and Biotech; Estados Unidos
Fil: ROSS, Pablo Juan. University of California; Estados Unidos
description High demand exists among commercial cattle producers for in vitro-derived bovine embryos fertilised with female sex-sorted spermatozoa from high-value breeding stock. The aim of this study was to evaluate three fertilisation media, namely M199, synthetic oviductal fluid (SOF) and Tyrode's albumin-lactate-pyruvate (TALP), on IVF performance using female sex-sorted spermatozoa. In all, 1143, 1220 and 1041 cumulus-oocyte complexes were fertilised in M199, SOF and TALP, respectively. There were significant differences among fertilisation media (P<0.05) in cleavage rate (M199≤57%, SOF≤71% and TALP≤72%), blastocyst formation (M199≤9%, SOF≤20% and TALP≤19%), proportion of Grade 1 blastocysts (M199≤15%, SOF≤52% and TALP≤51%), proportion of Grade 3 blastocysts (M199≤58%, SOF≤21% and TALP≤20%) and hatching rates (M199≤29%, SOF≤60% and TALP≤65%). The inner cell mass (ICM) and trophectoderm (TE) cells of Day 7 blastocysts were also affected by the fertilisation medium. Embryos derived from SOF and TALP fertilisation media had higher numbers of ICM, TE and total cells than those fertilised in M199. In conclusion, fertilisation media affected cleavage rate, as well as subsequent embryo development, quality and hatching ability. SOF and TALP fertilisation media produced significantly more embryos of higher quality than M199.
publishDate 2016
dc.date.none.fl_str_mv 2016-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/52574
Ferré, Luis Bernardo; Bogliotti, Yanina; Chitwood, James L.; Fresno Rodríguez, Cristóbal; Ortega, Hugo Hector; et al.; Comparison of different fertilisation media for an in vitro maturation-fertilisation-culture system using flow-cytometrically sorted X chromosome-bearing spermatozoa for bovine embryo production; Csiro Publishing; Reproduction Fertility and Development; 28; 11; 11-2016; 1695-1703
1031-3613
CONICET Digital
CONICET
url http://hdl.handle.net/11336/52574
identifier_str_mv Ferré, Luis Bernardo; Bogliotti, Yanina; Chitwood, James L.; Fresno Rodríguez, Cristóbal; Ortega, Hugo Hector; et al.; Comparison of different fertilisation media for an in vitro maturation-fertilisation-culture system using flow-cytometrically sorted X chromosome-bearing spermatozoa for bovine embryo production; Csiro Publishing; Reproduction Fertility and Development; 28; 11; 11-2016; 1695-1703
1031-3613
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1071/RD15019
info:eu-repo/semantics/altIdentifier/url/http://www.publish.csiro.au/rd/RD15019
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Csiro Publishing
publisher.none.fl_str_mv Csiro Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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