Human tubal secretion can modify the affinity of human spermatozoa for the zona pellucida

Autores
Munuce, María José; Serravalle, Analía; Caille, Adriana M.; Zumoffen, Carlos María; Botti, Gustavo; Cabada, Marcelo Oscar; Ghersevich, Sergio Albino
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: To study the effect of the human tubal tissue conditioned medium (CM) on sperm parameters related to sperm-zona pellucida interaction. Design: Controlled experimental laboratory study. Setting: Research laboratory. Subject(s): Semen samples from donors with normozoospermia. Human tubal tissue obtained from women undergoing hysterectomies. Human follicular fluids (hFF) and oocytes collected from patients undergoing IVF-ET. Intervention(s): Incubation of spermatozoa with CM proteins obtained from human tubal tissue culture; sperm binding to the zona pellucida assessment. Main Outcome Measure(s): Explants' viability was assessed by tissue DNA analysis. Sperm ability to interact with zona was tested with use of the whole oocyte test. Expression of d-mannose binding sites was assessed with use of a fluorescent probe on mannose coupled to bovine serum albumin. Human FF-induced acrosome reaction was assessed by the Pisum sativum technique. Result(s): Although treatment with 0.8 μg/μL of CM allowed sperm binding to the zona and the expression of d-mannose binding sites comparable with sperm in control medium, with 3.2 μg/mL of CM resulted in a significant decrease of both parameters. No effect of CM on spontaneous or hFF-induced acrosome reaction or in sperm viability was observed. Conclusion(s): The results indicate that the incubation of spermatozoa in the presence of CM reduces sperm affinity for the zona pellucida. This effect can be partly explained by the decreased expression of d-mannose binding sites on the sperm surface.
Fil: Munuce, María José. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Serravalle, Analía. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Caille, Adriana M.. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Zumoffen, Carlos María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Botti, Gustavo. Universidad Nacional de Rosario; Argentina
Fil: Cabada, Marcelo Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Ghersevich, Sergio Albino. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Materia
Acrosome Reaction
D-Mannose Binding Site
Human Oviduct
Human Spermatozoa
Proteins
Zona Pellucida Binding
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/80479
Acceder
id CONICETDig_70ead959b65c0d8e7959a74ed7715146
oai_identifier_str oai:ri.conicet.gov.ar:11336/80479
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Human tubal secretion can modify the affinity of human spermatozoa for the zona pellucidaMunuce, María JoséSerravalle, AnalíaCaille, Adriana M.Zumoffen, Carlos MaríaBotti, GustavoCabada, Marcelo OscarGhersevich, Sergio AlbinoAcrosome ReactionD-Mannose Binding SiteHuman OviductHuman SpermatozoaProteinsZona Pellucida Bindinghttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Objective: To study the effect of the human tubal tissue conditioned medium (CM) on sperm parameters related to sperm-zona pellucida interaction. Design: Controlled experimental laboratory study. Setting: Research laboratory. Subject(s): Semen samples from donors with normozoospermia. Human tubal tissue obtained from women undergoing hysterectomies. Human follicular fluids (hFF) and oocytes collected from patients undergoing IVF-ET. Intervention(s): Incubation of spermatozoa with CM proteins obtained from human tubal tissue culture; sperm binding to the zona pellucida assessment. Main Outcome Measure(s): Explants' viability was assessed by tissue DNA analysis. Sperm ability to interact with zona was tested with use of the whole oocyte test. Expression of d-mannose binding sites was assessed with use of a fluorescent probe on mannose coupled to bovine serum albumin. Human FF-induced acrosome reaction was assessed by the Pisum sativum technique. Result(s): Although treatment with 0.8 μg/μL of CM allowed sperm binding to the zona and the expression of d-mannose binding sites comparable with sperm in control medium, with 3.2 μg/mL of CM resulted in a significant decrease of both parameters. No effect of CM on spontaneous or hFF-induced acrosome reaction or in sperm viability was observed. Conclusion(s): The results indicate that the incubation of spermatozoa in the presence of CM reduces sperm affinity for the zona pellucida. This effect can be partly explained by the decreased expression of d-mannose binding sites on the sperm surface.Fil: Munuce, María José. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Serravalle, Analía. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Caille, Adriana M.. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Zumoffen, Carlos María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Botti, Gustavo. Universidad Nacional de Rosario; ArgentinaFil: Cabada, Marcelo Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Ghersevich, Sergio Albino. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaElsevier Science Inc2009-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/80479Munuce, María José; Serravalle, Analía; Caille, Adriana M.; Zumoffen, Carlos María; Botti, Gustavo; et al.; Human tubal secretion can modify the affinity of human spermatozoa for the zona pellucida; Elsevier Science Inc; Fertility and Sterility; 91; 2; 2-2009; 407-4130015-0282CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.fertnstert.2007.11.077info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0015028207041428info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:00:26Zoai:ri.conicet.gov.ar:11336/80479instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:00:27.049CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Human tubal secretion can modify the affinity of human spermatozoa for the zona pellucida
title Human tubal secretion can modify the affinity of human spermatozoa for the zona pellucida
spellingShingle Human tubal secretion can modify the affinity of human spermatozoa for the zona pellucida
Munuce, María José
Acrosome Reaction
D-Mannose Binding Site
Human Oviduct
Human Spermatozoa
Proteins
Zona Pellucida Binding
title_short Human tubal secretion can modify the affinity of human spermatozoa for the zona pellucida
title_full Human tubal secretion can modify the affinity of human spermatozoa for the zona pellucida
title_fullStr Human tubal secretion can modify the affinity of human spermatozoa for the zona pellucida
title_full_unstemmed Human tubal secretion can modify the affinity of human spermatozoa for the zona pellucida
title_sort Human tubal secretion can modify the affinity of human spermatozoa for the zona pellucida
dc.creator.none.fl_str_mv Munuce, María José
Serravalle, Analía
Caille, Adriana M.
Zumoffen, Carlos María
Botti, Gustavo
Cabada, Marcelo Oscar
Ghersevich, Sergio Albino
author Munuce, María José
author_facet Munuce, María José
Serravalle, Analía
Caille, Adriana M.
Zumoffen, Carlos María
Botti, Gustavo
Cabada, Marcelo Oscar
Ghersevich, Sergio Albino
author_role author
author2 Serravalle, Analía
Caille, Adriana M.
Zumoffen, Carlos María
Botti, Gustavo
Cabada, Marcelo Oscar
Ghersevich, Sergio Albino
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Acrosome Reaction
D-Mannose Binding Site
Human Oviduct
Human Spermatozoa
Proteins
Zona Pellucida Binding
topic Acrosome Reaction
D-Mannose Binding Site
Human Oviduct
Human Spermatozoa
Proteins
Zona Pellucida Binding
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Objective: To study the effect of the human tubal tissue conditioned medium (CM) on sperm parameters related to sperm-zona pellucida interaction. Design: Controlled experimental laboratory study. Setting: Research laboratory. Subject(s): Semen samples from donors with normozoospermia. Human tubal tissue obtained from women undergoing hysterectomies. Human follicular fluids (hFF) and oocytes collected from patients undergoing IVF-ET. Intervention(s): Incubation of spermatozoa with CM proteins obtained from human tubal tissue culture; sperm binding to the zona pellucida assessment. Main Outcome Measure(s): Explants' viability was assessed by tissue DNA analysis. Sperm ability to interact with zona was tested with use of the whole oocyte test. Expression of d-mannose binding sites was assessed with use of a fluorescent probe on mannose coupled to bovine serum albumin. Human FF-induced acrosome reaction was assessed by the Pisum sativum technique. Result(s): Although treatment with 0.8 μg/μL of CM allowed sperm binding to the zona and the expression of d-mannose binding sites comparable with sperm in control medium, with 3.2 μg/mL of CM resulted in a significant decrease of both parameters. No effect of CM on spontaneous or hFF-induced acrosome reaction or in sperm viability was observed. Conclusion(s): The results indicate that the incubation of spermatozoa in the presence of CM reduces sperm affinity for the zona pellucida. This effect can be partly explained by the decreased expression of d-mannose binding sites on the sperm surface.
Fil: Munuce, María José. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Serravalle, Analía. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Caille, Adriana M.. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Zumoffen, Carlos María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Botti, Gustavo. Universidad Nacional de Rosario; Argentina
Fil: Cabada, Marcelo Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Ghersevich, Sergio Albino. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
description Objective: To study the effect of the human tubal tissue conditioned medium (CM) on sperm parameters related to sperm-zona pellucida interaction. Design: Controlled experimental laboratory study. Setting: Research laboratory. Subject(s): Semen samples from donors with normozoospermia. Human tubal tissue obtained from women undergoing hysterectomies. Human follicular fluids (hFF) and oocytes collected from patients undergoing IVF-ET. Intervention(s): Incubation of spermatozoa with CM proteins obtained from human tubal tissue culture; sperm binding to the zona pellucida assessment. Main Outcome Measure(s): Explants' viability was assessed by tissue DNA analysis. Sperm ability to interact with zona was tested with use of the whole oocyte test. Expression of d-mannose binding sites was assessed with use of a fluorescent probe on mannose coupled to bovine serum albumin. Human FF-induced acrosome reaction was assessed by the Pisum sativum technique. Result(s): Although treatment with 0.8 μg/μL of CM allowed sperm binding to the zona and the expression of d-mannose binding sites comparable with sperm in control medium, with 3.2 μg/mL of CM resulted in a significant decrease of both parameters. No effect of CM on spontaneous or hFF-induced acrosome reaction or in sperm viability was observed. Conclusion(s): The results indicate that the incubation of spermatozoa in the presence of CM reduces sperm affinity for the zona pellucida. This effect can be partly explained by the decreased expression of d-mannose binding sites on the sperm surface.
publishDate 2009
dc.date.none.fl_str_mv 2009-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/80479
Munuce, María José; Serravalle, Analía; Caille, Adriana M.; Zumoffen, Carlos María; Botti, Gustavo; et al.; Human tubal secretion can modify the affinity of human spermatozoa for the zona pellucida; Elsevier Science Inc; Fertility and Sterility; 91; 2; 2-2009; 407-413
0015-0282
CONICET Digital
CONICET
url http://hdl.handle.net/11336/80479
identifier_str_mv Munuce, María José; Serravalle, Analía; Caille, Adriana M.; Zumoffen, Carlos María; Botti, Gustavo; et al.; Human tubal secretion can modify the affinity of human spermatozoa for the zona pellucida; Elsevier Science Inc; Fertility and Sterility; 91; 2; 2-2009; 407-413
0015-0282
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fertnstert.2007.11.077
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0015028207041428
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.221938

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