Reactive oxygen species-mediated degradation of antidiabetic compounds: Cytotoxic implications of their photodegradation products

Autores
Challier, Cecilia; Laurella, Sergio Luis; Allegretti, Patricia Ercilia; Sabini, Maria Carola; Sabini, Liliana Ines; Garcia, Norman Andino; Biasutti, Maria Alicia; Criado, Susana Noemi
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Reactive oxygen species (ROS) have been described in their double physiological function, helping in the maintenance of health as well as contributing to oxidative stress. Diabetes mellitus is a chronical disease nearly related to oxidative stress, whose treatment (in type II variant) consists in the administration of antidiabetic compounds (Andb) such as Gliclazide (Gli) and Glipizide (Glip). In this context, as Andb may be exposed to high ROS concentrations in diabetic patients, we have studied the potential ROS-mediated degradation of Gli and Glip through photosensitized processes, in the presence of Riboflavin (Rf) vitamin. We found that singlet oxygen (O 2 ( 1 ∆ g )) participated in the Rf-sensitized photodegradation of both Andb, and also superoxide radical anion in the case of Gli. Two principal products derived from O 2 ( 1 ∆ g )-mediated degradation of Gli were identified and their chemical structures characterized, through HPLC mass spectrometry. O 2 ( 1 ∆ g )-mediated degradation products and their toxicity was assayed on Vero cell line. These studies demonstrated that neither Gli nor its photoproducts caused cytotoxic effect under the experimental conditions assayed. Our results show strong evidences of ROS-mediated Andb degradation, which may involve the reduction or loss of their therapeutic action, as well as potential cytotoxicity derived from their oxidation products.
Fil: Challier, Cecilia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Laurella, Sergio Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina
Fil: Allegretti, Patricia Ercilia. Universidad Nacional de La Plata; Argentina
Fil: Sabini, Maria Carola. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Sabini, Liliana Ines. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Garcia, Norman Andino. Universidad Nacional de Río Cuarto; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Biasutti, Maria Alicia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Universidad Nacional de Río Cuarto; Argentina
Fil: Criado, Susana Noemi. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Universidad Nacional de Río Cuarto; Argentina
Materia
REACTIVE OXYGEN SPECIES
ANTIDIABETIC COMPOUNDS
DIABETES MELLITUS
CYTOTOXICITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/91972
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/91972
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Reactive oxygen species-mediated degradation of antidiabetic compounds: Cytotoxic implications of their photodegradation productsChallier, CeciliaLaurella, Sergio LuisAllegretti, Patricia ErciliaSabini, Maria CarolaSabini, Liliana InesGarcia, Norman AndinoBiasutti, Maria AliciaCriado, Susana NoemiREACTIVE OXYGEN SPECIESANTIDIABETIC COMPOUNDSDIABETES MELLITUSCYTOTOXICITYhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Reactive oxygen species (ROS) have been described in their double physiological function, helping in the maintenance of health as well as contributing to oxidative stress. Diabetes mellitus is a chronical disease nearly related to oxidative stress, whose treatment (in type II variant) consists in the administration of antidiabetic compounds (Andb) such as Gliclazide (Gli) and Glipizide (Glip). In this context, as Andb may be exposed to high ROS concentrations in diabetic patients, we have studied the potential ROS-mediated degradation of Gli and Glip through photosensitized processes, in the presence of Riboflavin (Rf) vitamin. We found that singlet oxygen (O 2 ( 1 ∆ g )) participated in the Rf-sensitized photodegradation of both Andb, and also superoxide radical anion in the case of Gli. Two principal products derived from O 2 ( 1 ∆ g )-mediated degradation of Gli were identified and their chemical structures characterized, through HPLC mass spectrometry. O 2 ( 1 ∆ g )-mediated degradation products and their toxicity was assayed on Vero cell line. These studies demonstrated that neither Gli nor its photoproducts caused cytotoxic effect under the experimental conditions assayed. Our results show strong evidences of ROS-mediated Andb degradation, which may involve the reduction or loss of their therapeutic action, as well as potential cytotoxicity derived from their oxidation products.Fil: Challier, Cecilia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Laurella, Sergio Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; ArgentinaFil: Allegretti, Patricia Ercilia. Universidad Nacional de La Plata; ArgentinaFil: Sabini, Maria Carola. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Sabini, Liliana Ines. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Garcia, Norman Andino. Universidad Nacional de Río Cuarto; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Biasutti, Maria Alicia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Universidad Nacional de Río Cuarto; ArgentinaFil: Criado, Susana Noemi. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Universidad Nacional de Río Cuarto; ArgentinaWiley Blackwell Publishing, Inc2018-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/91972Challier, Cecilia; Laurella, Sergio Luis; Allegretti, Patricia Ercilia; Sabini, Maria Carola; Sabini, Liliana Ines; et al.; Reactive oxygen species-mediated degradation of antidiabetic compounds: Cytotoxic implications of their photodegradation products; Wiley Blackwell Publishing, Inc; Photochemistry and Photobiology; 94; 6; 11-2018; 1151-11580031-86551751-1097CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://doi.wiley.com/10.1111/php.12989info:eu-repo/semantics/altIdentifier/doi/10.1111/php.12989info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/php.12989info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:19:40Zoai:ri.conicet.gov.ar:11336/91972instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:19:41.031CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Reactive oxygen species-mediated degradation of antidiabetic compounds: Cytotoxic implications of their photodegradation products
title Reactive oxygen species-mediated degradation of antidiabetic compounds: Cytotoxic implications of their photodegradation products
spellingShingle Reactive oxygen species-mediated degradation of antidiabetic compounds: Cytotoxic implications of their photodegradation products
Challier, Cecilia
REACTIVE OXYGEN SPECIES
ANTIDIABETIC COMPOUNDS
DIABETES MELLITUS
CYTOTOXICITY
title_short Reactive oxygen species-mediated degradation of antidiabetic compounds: Cytotoxic implications of their photodegradation products
title_full Reactive oxygen species-mediated degradation of antidiabetic compounds: Cytotoxic implications of their photodegradation products
title_fullStr Reactive oxygen species-mediated degradation of antidiabetic compounds: Cytotoxic implications of their photodegradation products
title_full_unstemmed Reactive oxygen species-mediated degradation of antidiabetic compounds: Cytotoxic implications of their photodegradation products
title_sort Reactive oxygen species-mediated degradation of antidiabetic compounds: Cytotoxic implications of their photodegradation products
dc.creator.none.fl_str_mv Challier, Cecilia
Laurella, Sergio Luis
Allegretti, Patricia Ercilia
Sabini, Maria Carola
Sabini, Liliana Ines
Garcia, Norman Andino
Biasutti, Maria Alicia
Criado, Susana Noemi
author Challier, Cecilia
author_facet Challier, Cecilia
Laurella, Sergio Luis
Allegretti, Patricia Ercilia
Sabini, Maria Carola
Sabini, Liliana Ines
Garcia, Norman Andino
Biasutti, Maria Alicia
Criado, Susana Noemi
author_role author
author2 Laurella, Sergio Luis
Allegretti, Patricia Ercilia
Sabini, Maria Carola
Sabini, Liliana Ines
Garcia, Norman Andino
Biasutti, Maria Alicia
Criado, Susana Noemi
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv REACTIVE OXYGEN SPECIES
ANTIDIABETIC COMPOUNDS
DIABETES MELLITUS
CYTOTOXICITY
topic REACTIVE OXYGEN SPECIES
ANTIDIABETIC COMPOUNDS
DIABETES MELLITUS
CYTOTOXICITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Reactive oxygen species (ROS) have been described in their double physiological function, helping in the maintenance of health as well as contributing to oxidative stress. Diabetes mellitus is a chronical disease nearly related to oxidative stress, whose treatment (in type II variant) consists in the administration of antidiabetic compounds (Andb) such as Gliclazide (Gli) and Glipizide (Glip). In this context, as Andb may be exposed to high ROS concentrations in diabetic patients, we have studied the potential ROS-mediated degradation of Gli and Glip through photosensitized processes, in the presence of Riboflavin (Rf) vitamin. We found that singlet oxygen (O 2 ( 1 ∆ g )) participated in the Rf-sensitized photodegradation of both Andb, and also superoxide radical anion in the case of Gli. Two principal products derived from O 2 ( 1 ∆ g )-mediated degradation of Gli were identified and their chemical structures characterized, through HPLC mass spectrometry. O 2 ( 1 ∆ g )-mediated degradation products and their toxicity was assayed on Vero cell line. These studies demonstrated that neither Gli nor its photoproducts caused cytotoxic effect under the experimental conditions assayed. Our results show strong evidences of ROS-mediated Andb degradation, which may involve the reduction or loss of their therapeutic action, as well as potential cytotoxicity derived from their oxidation products.
Fil: Challier, Cecilia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Laurella, Sergio Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina
Fil: Allegretti, Patricia Ercilia. Universidad Nacional de La Plata; Argentina
Fil: Sabini, Maria Carola. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Sabini, Liliana Ines. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Garcia, Norman Andino. Universidad Nacional de Río Cuarto; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Biasutti, Maria Alicia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Universidad Nacional de Río Cuarto; Argentina
Fil: Criado, Susana Noemi. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Universidad Nacional de Río Cuarto; Argentina
description Reactive oxygen species (ROS) have been described in their double physiological function, helping in the maintenance of health as well as contributing to oxidative stress. Diabetes mellitus is a chronical disease nearly related to oxidative stress, whose treatment (in type II variant) consists in the administration of antidiabetic compounds (Andb) such as Gliclazide (Gli) and Glipizide (Glip). In this context, as Andb may be exposed to high ROS concentrations in diabetic patients, we have studied the potential ROS-mediated degradation of Gli and Glip through photosensitized processes, in the presence of Riboflavin (Rf) vitamin. We found that singlet oxygen (O 2 ( 1 ∆ g )) participated in the Rf-sensitized photodegradation of both Andb, and also superoxide radical anion in the case of Gli. Two principal products derived from O 2 ( 1 ∆ g )-mediated degradation of Gli were identified and their chemical structures characterized, through HPLC mass spectrometry. O 2 ( 1 ∆ g )-mediated degradation products and their toxicity was assayed on Vero cell line. These studies demonstrated that neither Gli nor its photoproducts caused cytotoxic effect under the experimental conditions assayed. Our results show strong evidences of ROS-mediated Andb degradation, which may involve the reduction or loss of their therapeutic action, as well as potential cytotoxicity derived from their oxidation products.
publishDate 2018
dc.date.none.fl_str_mv 2018-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/91972
Challier, Cecilia; Laurella, Sergio Luis; Allegretti, Patricia Ercilia; Sabini, Maria Carola; Sabini, Liliana Ines; et al.; Reactive oxygen species-mediated degradation of antidiabetic compounds: Cytotoxic implications of their photodegradation products; Wiley Blackwell Publishing, Inc; Photochemistry and Photobiology; 94; 6; 11-2018; 1151-1158
0031-8655
1751-1097
CONICET Digital
CONICET
url http://hdl.handle.net/11336/91972
identifier_str_mv Challier, Cecilia; Laurella, Sergio Luis; Allegretti, Patricia Ercilia; Sabini, Maria Carola; Sabini, Liliana Ines; et al.; Reactive oxygen species-mediated degradation of antidiabetic compounds: Cytotoxic implications of their photodegradation products; Wiley Blackwell Publishing, Inc; Photochemistry and Photobiology; 94; 6; 11-2018; 1151-1158
0031-8655
1751-1097
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://doi.wiley.com/10.1111/php.12989
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info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/php.12989
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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