Antidiabetic potential of extracts obtained from different varieties of sorghum
- Autores
- Escobar, Ana Paula; Gonzalez Miragliotta, Ana Melissa; Ojeda, Gonzalo Adrian; Gonzalez, Romina Belen; Torres, Ana María; Aguirre, María Victoria
- Año de publicación
- 2023
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Type 2 diabetes mellitus is a chronic condition that covers approximately 90-95% of cases. In the current market, there is a wide variety of drugs for its treatment. In recent years there has been a growing interest in alternative approaches such as the use of natural products. There are references about the usefulness of sorghum as a hypoglycemic agent referred to the species cultivated in other regions of the world, but the study of the species adapted to our region (NEA) is of the utmost importance since it is known that they can vary their chemical composition due to edaphoclimatological influences. The objective of this work was to evaluate the hypoglycemic potential of sorghum by measuring the inhibitory capacity of sorghum extracts on the enzyme alpha glucosidase (APG) responsible for glucose absorption at the intestinal level. Samples of red and white variety sorghum were used with 3 different particle sizes for each case, on a 500um sieve (ST500), 500um flour (H500) and 177um fine flour (HF177). The material was macerated for 48h in a previously optimized mixture of solvents, filtered and dried in a rotatory evaporatorThe in vitro APG inhibition assay was performed in a microplate reader using extracts (0.02mg/ml) and acarbose (1mg/ml) as positive control. The final results were expressed as the inhibition ratio in relation to acarbose. It was observed that all the extracts were more active than acarbose (Ir>1). However, the red variety presented greater inhibitory capacity than the white variety. For red sorghum, the most active extract was ST500 (ir=75.68) followed by H500 (Ir= 56.31). In the case of white sorghum, the extract that was most active was ST500 (Ir=46.96) but lower than all the cases of red sorghum. Our findings show that there are significant differences in the hypoglycemic potential of sorghum extracts according to the variety and size of the material used.
Fil: Escobar, Ana Paula. Universidad Nacional del Nordeste; Argentina
Fil: Gonzalez Miragliotta, Ana Melissa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: Ojeda, Gonzalo Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: Gonzalez, Romina Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: Torres, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: Aguirre, María Victoria. Universidad Nacional del Nordeste. Facultad de Medicina. Laboratorio de Investigaciones Bioquímicas; Argentina
LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Farmacología Experimental
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Sociedad Argentina de Biología - Materia
-
Sorghum
Diabetes
Antidiabetic
Optimization - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/246734
Ver los metadatos del registro completo
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Antidiabetic potential of extracts obtained from different varieties of sorghumEscobar, Ana PaulaGonzalez Miragliotta, Ana MelissaOjeda, Gonzalo AdrianGonzalez, Romina BelenTorres, Ana MaríaAguirre, María VictoriaSorghumDiabetesAntidiabeticOptimizationhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Type 2 diabetes mellitus is a chronic condition that covers approximately 90-95% of cases. In the current market, there is a wide variety of drugs for its treatment. In recent years there has been a growing interest in alternative approaches such as the use of natural products. There are references about the usefulness of sorghum as a hypoglycemic agent referred to the species cultivated in other regions of the world, but the study of the species adapted to our region (NEA) is of the utmost importance since it is known that they can vary their chemical composition due to edaphoclimatological influences. The objective of this work was to evaluate the hypoglycemic potential of sorghum by measuring the inhibitory capacity of sorghum extracts on the enzyme alpha glucosidase (APG) responsible for glucose absorption at the intestinal level. Samples of red and white variety sorghum were used with 3 different particle sizes for each case, on a 500um sieve (ST500), 500um flour (H500) and 177um fine flour (HF177). The material was macerated for 48h in a previously optimized mixture of solvents, filtered and dried in a rotatory evaporatorThe in vitro APG inhibition assay was performed in a microplate reader using extracts (0.02mg/ml) and acarbose (1mg/ml) as positive control. The final results were expressed as the inhibition ratio in relation to acarbose. It was observed that all the extracts were more active than acarbose (Ir>1). However, the red variety presented greater inhibitory capacity than the white variety. For red sorghum, the most active extract was ST500 (ir=75.68) followed by H500 (Ir= 56.31). In the case of white sorghum, the extract that was most active was ST500 (Ir=46.96) but lower than all the cases of red sorghum. Our findings show that there are significant differences in the hypoglycemic potential of sorghum extracts according to the variety and size of the material used.Fil: Escobar, Ana Paula. Universidad Nacional del Nordeste; ArgentinaFil: Gonzalez Miragliotta, Ana Melissa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; ArgentinaFil: Ojeda, Gonzalo Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; ArgentinaFil: Gonzalez, Romina Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; ArgentinaFil: Torres, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; ArgentinaFil: Aguirre, María Victoria. Universidad Nacional del Nordeste. Facultad de Medicina. Laboratorio de Investigaciones Bioquímicas; ArgentinaLXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de LaboratorioMar del PlataArgentinaSociedad Argentina de Investigación ClínicaAsociación Argentina de Farmacología ExperimentalAsociación Argentina de Ciencia y Tecnología de Animales de LaboratorioSociedad Argentina de BiologíaFundación Revista Medicina2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/246734Antidiabetic potential of extracts obtained from different varieties of sorghum; LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2023; 221-2211669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.cominfo:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/revista-medicinaNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:00:25Zoai:ri.conicet.gov.ar:11336/246734instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:00:25.588CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Antidiabetic potential of extracts obtained from different varieties of sorghum |
title |
Antidiabetic potential of extracts obtained from different varieties of sorghum |
spellingShingle |
Antidiabetic potential of extracts obtained from different varieties of sorghum Escobar, Ana Paula Sorghum Diabetes Antidiabetic Optimization |
title_short |
Antidiabetic potential of extracts obtained from different varieties of sorghum |
title_full |
Antidiabetic potential of extracts obtained from different varieties of sorghum |
title_fullStr |
Antidiabetic potential of extracts obtained from different varieties of sorghum |
title_full_unstemmed |
Antidiabetic potential of extracts obtained from different varieties of sorghum |
title_sort |
Antidiabetic potential of extracts obtained from different varieties of sorghum |
dc.creator.none.fl_str_mv |
Escobar, Ana Paula Gonzalez Miragliotta, Ana Melissa Ojeda, Gonzalo Adrian Gonzalez, Romina Belen Torres, Ana María Aguirre, María Victoria |
author |
Escobar, Ana Paula |
author_facet |
Escobar, Ana Paula Gonzalez Miragliotta, Ana Melissa Ojeda, Gonzalo Adrian Gonzalez, Romina Belen Torres, Ana María Aguirre, María Victoria |
author_role |
author |
author2 |
Gonzalez Miragliotta, Ana Melissa Ojeda, Gonzalo Adrian Gonzalez, Romina Belen Torres, Ana María Aguirre, María Victoria |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Sorghum Diabetes Antidiabetic Optimization |
topic |
Sorghum Diabetes Antidiabetic Optimization |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Type 2 diabetes mellitus is a chronic condition that covers approximately 90-95% of cases. In the current market, there is a wide variety of drugs for its treatment. In recent years there has been a growing interest in alternative approaches such as the use of natural products. There are references about the usefulness of sorghum as a hypoglycemic agent referred to the species cultivated in other regions of the world, but the study of the species adapted to our region (NEA) is of the utmost importance since it is known that they can vary their chemical composition due to edaphoclimatological influences. The objective of this work was to evaluate the hypoglycemic potential of sorghum by measuring the inhibitory capacity of sorghum extracts on the enzyme alpha glucosidase (APG) responsible for glucose absorption at the intestinal level. Samples of red and white variety sorghum were used with 3 different particle sizes for each case, on a 500um sieve (ST500), 500um flour (H500) and 177um fine flour (HF177). The material was macerated for 48h in a previously optimized mixture of solvents, filtered and dried in a rotatory evaporatorThe in vitro APG inhibition assay was performed in a microplate reader using extracts (0.02mg/ml) and acarbose (1mg/ml) as positive control. The final results were expressed as the inhibition ratio in relation to acarbose. It was observed that all the extracts were more active than acarbose (Ir>1). However, the red variety presented greater inhibitory capacity than the white variety. For red sorghum, the most active extract was ST500 (ir=75.68) followed by H500 (Ir= 56.31). In the case of white sorghum, the extract that was most active was ST500 (Ir=46.96) but lower than all the cases of red sorghum. Our findings show that there are significant differences in the hypoglycemic potential of sorghum extracts according to the variety and size of the material used. Fil: Escobar, Ana Paula. Universidad Nacional del Nordeste; Argentina Fil: Gonzalez Miragliotta, Ana Melissa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina Fil: Ojeda, Gonzalo Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina Fil: Gonzalez, Romina Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina Fil: Torres, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina Fil: Aguirre, María Victoria. Universidad Nacional del Nordeste. Facultad de Medicina. Laboratorio de Investigaciones Bioquímicas; Argentina LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio Mar del Plata Argentina Sociedad Argentina de Investigación Clínica Asociación Argentina de Farmacología Experimental Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio Sociedad Argentina de Biología |
description |
Type 2 diabetes mellitus is a chronic condition that covers approximately 90-95% of cases. In the current market, there is a wide variety of drugs for its treatment. In recent years there has been a growing interest in alternative approaches such as the use of natural products. There are references about the usefulness of sorghum as a hypoglycemic agent referred to the species cultivated in other regions of the world, but the study of the species adapted to our region (NEA) is of the utmost importance since it is known that they can vary their chemical composition due to edaphoclimatological influences. The objective of this work was to evaluate the hypoglycemic potential of sorghum by measuring the inhibitory capacity of sorghum extracts on the enzyme alpha glucosidase (APG) responsible for glucose absorption at the intestinal level. Samples of red and white variety sorghum were used with 3 different particle sizes for each case, on a 500um sieve (ST500), 500um flour (H500) and 177um fine flour (HF177). The material was macerated for 48h in a previously optimized mixture of solvents, filtered and dried in a rotatory evaporatorThe in vitro APG inhibition assay was performed in a microplate reader using extracts (0.02mg/ml) and acarbose (1mg/ml) as positive control. The final results were expressed as the inhibition ratio in relation to acarbose. It was observed that all the extracts were more active than acarbose (Ir>1). However, the red variety presented greater inhibitory capacity than the white variety. For red sorghum, the most active extract was ST500 (ir=75.68) followed by H500 (Ir= 56.31). In the case of white sorghum, the extract that was most active was ST500 (Ir=46.96) but lower than all the cases of red sorghum. Our findings show that there are significant differences in the hypoglycemic potential of sorghum extracts according to the variety and size of the material used. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023 |
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info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Reunión Journal http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
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publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/246734 Antidiabetic potential of extracts obtained from different varieties of sorghum; LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2023; 221-221 1669-9106 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/246734 |
identifier_str_mv |
Antidiabetic potential of extracts obtained from different varieties of sorghum; LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2023; 221-221 1669-9106 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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openAccess |
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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Fundación Revista Medicina |
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Fundación Revista Medicina |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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