Antidiabetic potential of extracts obtained from different varieties of sorghum

Autores
Escobar, Ana Paula; Gonzalez Miragliotta, Ana Melissa; Ojeda, Gonzalo Adrian; Gonzalez, Romina Belen; Torres, Ana María; Aguirre, María Victoria
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Type 2 diabetes mellitus is a chronic condition that covers approximately 90-95% of cases. In the current market, there is a wide variety of drugs for its treatment. In recent years there has been a growing interest in alternative approaches such as the use of natural products. There are references about the usefulness of sorghum as a hypoglycemic agent referred to the species cultivated in other regions of the world, but the study of the species adapted to our region (NEA) is of the utmost importance since it is known that they can vary their chemical composition due to edaphoclimatological influences. The objective of this work was to evaluate the hypoglycemic potential of sorghum by measuring the inhibitory capacity of sorghum extracts on the enzyme alpha glucosidase (APG) responsible for glucose absorption at the intestinal level. Samples of red and white variety sorghum were used with 3 different particle sizes for each case, on a 500um sieve (ST500), 500um flour (H500) and 177um fine flour (HF177). The material was macerated for 48h in a previously optimized mixture of solvents, filtered and dried in a rotatory evaporatorThe in vitro APG inhibition assay was performed in a microplate reader using extracts (0.02mg/ml) and acarbose (1mg/ml) as positive control. The final results were expressed as the inhibition ratio in relation to acarbose. It was observed that all the extracts were more active than acarbose (Ir>1). However, the red variety presented greater inhibitory capacity than the white variety. For red sorghum, the most active extract was ST500 (ir=75.68) followed by H500 (Ir= 56.31). In the case of white sorghum, the extract that was most active was ST500 (Ir=46.96) but lower than all the cases of red sorghum. Our findings show that there are significant differences in the hypoglycemic potential of sorghum extracts according to the variety and size of the material used.
Fil: Escobar, Ana Paula. Universidad Nacional del Nordeste; Argentina
Fil: Gonzalez Miragliotta, Ana Melissa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: Ojeda, Gonzalo Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: Gonzalez, Romina Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: Torres, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: Aguirre, María Victoria. Universidad Nacional del Nordeste. Facultad de Medicina. Laboratorio de Investigaciones Bioquímicas; Argentina
LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Farmacología Experimental
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Sociedad Argentina de Biología
Materia
Sorghum
Diabetes
Antidiabetic
Optimization
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/246734
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Antidiabetic potential of extracts obtained from different varieties of sorghumEscobar, Ana PaulaGonzalez Miragliotta, Ana MelissaOjeda, Gonzalo AdrianGonzalez, Romina BelenTorres, Ana MaríaAguirre, María VictoriaSorghumDiabetesAntidiabeticOptimizationhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Type 2 diabetes mellitus is a chronic condition that covers approximately 90-95% of cases. In the current market, there is a wide variety of drugs for its treatment. In recent years there has been a growing interest in alternative approaches such as the use of natural products. There are references about the usefulness of sorghum as a hypoglycemic agent referred to the species cultivated in other regions of the world, but the study of the species adapted to our region (NEA) is of the utmost importance since it is known that they can vary their chemical composition due to edaphoclimatological influences. The objective of this work was to evaluate the hypoglycemic potential of sorghum by measuring the inhibitory capacity of sorghum extracts on the enzyme alpha glucosidase (APG) responsible for glucose absorption at the intestinal level. Samples of red and white variety sorghum were used with 3 different particle sizes for each case, on a 500um sieve (ST500), 500um flour (H500) and 177um fine flour (HF177). The material was macerated for 48h in a previously optimized mixture of solvents, filtered and dried in a rotatory evaporatorThe in vitro APG inhibition assay was performed in a microplate reader using extracts (0.02mg/ml) and acarbose (1mg/ml) as positive control. The final results were expressed as the inhibition ratio in relation to acarbose. It was observed that all the extracts were more active than acarbose (Ir>1). However, the red variety presented greater inhibitory capacity than the white variety. For red sorghum, the most active extract was ST500 (ir=75.68) followed by H500 (Ir= 56.31). In the case of white sorghum, the extract that was most active was ST500 (Ir=46.96) but lower than all the cases of red sorghum. Our findings show that there are significant differences in the hypoglycemic potential of sorghum extracts according to the variety and size of the material used.Fil: Escobar, Ana Paula. Universidad Nacional del Nordeste; ArgentinaFil: Gonzalez Miragliotta, Ana Melissa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; ArgentinaFil: Ojeda, Gonzalo Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; ArgentinaFil: Gonzalez, Romina Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; ArgentinaFil: Torres, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; ArgentinaFil: Aguirre, María Victoria. Universidad Nacional del Nordeste. Facultad de Medicina. Laboratorio de Investigaciones Bioquímicas; ArgentinaLXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de LaboratorioMar del PlataArgentinaSociedad Argentina de Investigación ClínicaAsociación Argentina de Farmacología ExperimentalAsociación Argentina de Ciencia y Tecnología de Animales de LaboratorioSociedad Argentina de BiologíaFundación Revista Medicina2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/246734Antidiabetic potential of extracts obtained from different varieties of sorghum; LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2023; 221-2211669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.cominfo:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/revista-medicinaNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:29:54Zoai:ri.conicet.gov.ar:11336/246734instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:29:54.505CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antidiabetic potential of extracts obtained from different varieties of sorghum
title Antidiabetic potential of extracts obtained from different varieties of sorghum
spellingShingle Antidiabetic potential of extracts obtained from different varieties of sorghum
Escobar, Ana Paula
Sorghum
Diabetes
Antidiabetic
Optimization
title_short Antidiabetic potential of extracts obtained from different varieties of sorghum
title_full Antidiabetic potential of extracts obtained from different varieties of sorghum
title_fullStr Antidiabetic potential of extracts obtained from different varieties of sorghum
title_full_unstemmed Antidiabetic potential of extracts obtained from different varieties of sorghum
title_sort Antidiabetic potential of extracts obtained from different varieties of sorghum
dc.creator.none.fl_str_mv Escobar, Ana Paula
Gonzalez Miragliotta, Ana Melissa
Ojeda, Gonzalo Adrian
Gonzalez, Romina Belen
Torres, Ana María
Aguirre, María Victoria
author Escobar, Ana Paula
author_facet Escobar, Ana Paula
Gonzalez Miragliotta, Ana Melissa
Ojeda, Gonzalo Adrian
Gonzalez, Romina Belen
Torres, Ana María
Aguirre, María Victoria
author_role author
author2 Gonzalez Miragliotta, Ana Melissa
Ojeda, Gonzalo Adrian
Gonzalez, Romina Belen
Torres, Ana María
Aguirre, María Victoria
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Sorghum
Diabetes
Antidiabetic
Optimization
topic Sorghum
Diabetes
Antidiabetic
Optimization
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Type 2 diabetes mellitus is a chronic condition that covers approximately 90-95% of cases. In the current market, there is a wide variety of drugs for its treatment. In recent years there has been a growing interest in alternative approaches such as the use of natural products. There are references about the usefulness of sorghum as a hypoglycemic agent referred to the species cultivated in other regions of the world, but the study of the species adapted to our region (NEA) is of the utmost importance since it is known that they can vary their chemical composition due to edaphoclimatological influences. The objective of this work was to evaluate the hypoglycemic potential of sorghum by measuring the inhibitory capacity of sorghum extracts on the enzyme alpha glucosidase (APG) responsible for glucose absorption at the intestinal level. Samples of red and white variety sorghum were used with 3 different particle sizes for each case, on a 500um sieve (ST500), 500um flour (H500) and 177um fine flour (HF177). The material was macerated for 48h in a previously optimized mixture of solvents, filtered and dried in a rotatory evaporatorThe in vitro APG inhibition assay was performed in a microplate reader using extracts (0.02mg/ml) and acarbose (1mg/ml) as positive control. The final results were expressed as the inhibition ratio in relation to acarbose. It was observed that all the extracts were more active than acarbose (Ir>1). However, the red variety presented greater inhibitory capacity than the white variety. For red sorghum, the most active extract was ST500 (ir=75.68) followed by H500 (Ir= 56.31). In the case of white sorghum, the extract that was most active was ST500 (Ir=46.96) but lower than all the cases of red sorghum. Our findings show that there are significant differences in the hypoglycemic potential of sorghum extracts according to the variety and size of the material used.
Fil: Escobar, Ana Paula. Universidad Nacional del Nordeste; Argentina
Fil: Gonzalez Miragliotta, Ana Melissa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: Ojeda, Gonzalo Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: Gonzalez, Romina Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: Torres, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: Aguirre, María Victoria. Universidad Nacional del Nordeste. Facultad de Medicina. Laboratorio de Investigaciones Bioquímicas; Argentina
LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Farmacología Experimental
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Sociedad Argentina de Biología
description Type 2 diabetes mellitus is a chronic condition that covers approximately 90-95% of cases. In the current market, there is a wide variety of drugs for its treatment. In recent years there has been a growing interest in alternative approaches such as the use of natural products. There are references about the usefulness of sorghum as a hypoglycemic agent referred to the species cultivated in other regions of the world, but the study of the species adapted to our region (NEA) is of the utmost importance since it is known that they can vary their chemical composition due to edaphoclimatological influences. The objective of this work was to evaluate the hypoglycemic potential of sorghum by measuring the inhibitory capacity of sorghum extracts on the enzyme alpha glucosidase (APG) responsible for glucose absorption at the intestinal level. Samples of red and white variety sorghum were used with 3 different particle sizes for each case, on a 500um sieve (ST500), 500um flour (H500) and 177um fine flour (HF177). The material was macerated for 48h in a previously optimized mixture of solvents, filtered and dried in a rotatory evaporatorThe in vitro APG inhibition assay was performed in a microplate reader using extracts (0.02mg/ml) and acarbose (1mg/ml) as positive control. The final results were expressed as the inhibition ratio in relation to acarbose. It was observed that all the extracts were more active than acarbose (Ir>1). However, the red variety presented greater inhibitory capacity than the white variety. For red sorghum, the most active extract was ST500 (ir=75.68) followed by H500 (Ir= 56.31). In the case of white sorghum, the extract that was most active was ST500 (Ir=46.96) but lower than all the cases of red sorghum. Our findings show that there are significant differences in the hypoglycemic potential of sorghum extracts according to the variety and size of the material used.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/246734
Antidiabetic potential of extracts obtained from different varieties of sorghum; LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2023; 221-221
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/246734
identifier_str_mv Antidiabetic potential of extracts obtained from different varieties of sorghum; LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2023; 221-221
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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