Mal de Río Cuarto virus infection triggers the production of distinctive viral-derived siRNA profiles in wheat and its planthopper vector

Autores
de Haro, Luis Alejandro; Dumón, Analía Delina; Mattio, Maria Fernanda; Argüello Caro, Evangelina Beatriz; Llauger, Gabriela; Zavallo, Diego; Blanc, Hervé; Mongelli, Vanesa Claudia; Truol, Graciela Ana; Saleh, María Carla; Asurmendi, Sebastian; del Vas, Mariana
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Plant reoviruses are able to multiply in gramineae plants and delphacid vectors encountering different defense strategies with unique features. This study aims to comparatively assess alterations of small RNA (sRNA) populations in both hosts upon virus infection. For this purpose, we characterized the sRNA profiles of wheat and planthopper vectors infected by Mal de Río Cuarto virus (MRCV, Fijivirus, Reoviridae) and quantified virus genome segments by quantitative reverse transcription PCR We provide evidence that plant and insect silencing machineries differentially recognize the viral genome, thus giving rise to distinct profiles of virus-derived small interfering RNAs (vsiRNAs). In plants, most of the virus genome segments were targeted preferentially within their upstream sequences and vsiRNAs mapped with higher density to the smaller genome segments than to the medium or larger ones. This tendency, however, was not observed in insects. In both hosts, vsiRNAs were equally derived from sense and antisense RNA strands and the differences in vsiRNAs accumulation did not correlate with mRNAs accumulation. We also established that the piwi-interacting RNA (piRNA) pathway was active in the delphacid vector but, contrary to what is observed in virus-infected mosquitoes, virus-specific piRNAs were not detected. This work contributes to the understanding of the silencing response in insect and plant hosts.
Fil: de Haro, Luis Alejandro. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dumón, Analía Delina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mattio, Maria Fernanda. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Argüello Caro, Evangelina Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Llauger, Gabriela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zavallo, Diego. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Blanc, Hervé. Instituto Pasteur; Francia
Fil: Mongelli, Vanesa Claudia. Instituto Pasteur; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Truol, Graciela Ana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: Saleh, María Carla. Instituto Pasteur; Francia
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
FIJIVIRUS
MRCV
PIRNAS
PLANTHOPPER
RNA SILENCING
SRNAS
VSIRNAS
WHEAT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/72863
Acceder
id CONICETDig_8b66f377b3fa17b9942a70fee29fa50c
oai_identifier_str oai:ri.conicet.gov.ar:11336/72863
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Mal de Río Cuarto virus infection triggers the production of distinctive viral-derived siRNA profiles in wheat and its planthopper vectorde Haro, Luis AlejandroDumón, Analía DelinaMattio, Maria FernandaArgüello Caro, Evangelina BeatrizLlauger, GabrielaZavallo, DiegoBlanc, HervéMongelli, Vanesa ClaudiaTruol, Graciela AnaSaleh, María CarlaAsurmendi, Sebastiandel Vas, MarianaFIJIVIRUSMRCVPIRNASPLANTHOPPERRNA SILENCINGSRNASVSIRNASWHEAThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Plant reoviruses are able to multiply in gramineae plants and delphacid vectors encountering different defense strategies with unique features. This study aims to comparatively assess alterations of small RNA (sRNA) populations in both hosts upon virus infection. For this purpose, we characterized the sRNA profiles of wheat and planthopper vectors infected by Mal de Río Cuarto virus (MRCV, Fijivirus, Reoviridae) and quantified virus genome segments by quantitative reverse transcription PCR We provide evidence that plant and insect silencing machineries differentially recognize the viral genome, thus giving rise to distinct profiles of virus-derived small interfering RNAs (vsiRNAs). In plants, most of the virus genome segments were targeted preferentially within their upstream sequences and vsiRNAs mapped with higher density to the smaller genome segments than to the medium or larger ones. This tendency, however, was not observed in insects. In both hosts, vsiRNAs were equally derived from sense and antisense RNA strands and the differences in vsiRNAs accumulation did not correlate with mRNAs accumulation. We also established that the piwi-interacting RNA (piRNA) pathway was active in the delphacid vector but, contrary to what is observed in virus-infected mosquitoes, virus-specific piRNAs were not detected. This work contributes to the understanding of the silencing response in insect and plant hosts.Fil: de Haro, Luis Alejandro. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Dumón, Analía Delina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mattio, Maria Fernanda. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Argüello Caro, Evangelina Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Llauger, Gabriela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zavallo, Diego. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Blanc, Hervé. Instituto Pasteur; FranciaFil: Mongelli, Vanesa Claudia. Instituto Pasteur; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Truol, Graciela Ana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Saleh, María Carla. Instituto Pasteur; FranciaFil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFrontiers Research Foundation2017-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/72863de Haro, Luis Alejandro; Dumón, Analía Delina; Mattio, Maria Fernanda; Argüello Caro, Evangelina Beatriz; Llauger, Gabriela; et al.; Mal de Río Cuarto virus infection triggers the production of distinctive viral-derived siRNA profiles in wheat and its planthopper vector; Frontiers Research Foundation; Frontiers in Plant Science; 8; 5-2017; 1-111664-462XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2017.00766info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fpls.2017.00766/fullinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:55:38Zoai:ri.conicet.gov.ar:11336/72863instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:55:38.315CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mal de Río Cuarto virus infection triggers the production of distinctive viral-derived siRNA profiles in wheat and its planthopper vector
title Mal de Río Cuarto virus infection triggers the production of distinctive viral-derived siRNA profiles in wheat and its planthopper vector
spellingShingle Mal de Río Cuarto virus infection triggers the production of distinctive viral-derived siRNA profiles in wheat and its planthopper vector
de Haro, Luis Alejandro
FIJIVIRUS
MRCV
PIRNAS
PLANTHOPPER
RNA SILENCING
SRNAS
VSIRNAS
WHEAT
title_short Mal de Río Cuarto virus infection triggers the production of distinctive viral-derived siRNA profiles in wheat and its planthopper vector
title_full Mal de Río Cuarto virus infection triggers the production of distinctive viral-derived siRNA profiles in wheat and its planthopper vector
title_fullStr Mal de Río Cuarto virus infection triggers the production of distinctive viral-derived siRNA profiles in wheat and its planthopper vector
title_full_unstemmed Mal de Río Cuarto virus infection triggers the production of distinctive viral-derived siRNA profiles in wheat and its planthopper vector
title_sort Mal de Río Cuarto virus infection triggers the production of distinctive viral-derived siRNA profiles in wheat and its planthopper vector
dc.creator.none.fl_str_mv de Haro, Luis Alejandro
Dumón, Analía Delina
Mattio, Maria Fernanda
Argüello Caro, Evangelina Beatriz
Llauger, Gabriela
Zavallo, Diego
Blanc, Hervé
Mongelli, Vanesa Claudia
Truol, Graciela Ana
Saleh, María Carla
Asurmendi, Sebastian
del Vas, Mariana
author de Haro, Luis Alejandro
author_facet de Haro, Luis Alejandro
Dumón, Analía Delina
Mattio, Maria Fernanda
Argüello Caro, Evangelina Beatriz
Llauger, Gabriela
Zavallo, Diego
Blanc, Hervé
Mongelli, Vanesa Claudia
Truol, Graciela Ana
Saleh, María Carla
Asurmendi, Sebastian
del Vas, Mariana
author_role author
author2 Dumón, Analía Delina
Mattio, Maria Fernanda
Argüello Caro, Evangelina Beatriz
Llauger, Gabriela
Zavallo, Diego
Blanc, Hervé
Mongelli, Vanesa Claudia
Truol, Graciela Ana
Saleh, María Carla
Asurmendi, Sebastian
del Vas, Mariana
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv FIJIVIRUS
MRCV
PIRNAS
PLANTHOPPER
RNA SILENCING
SRNAS
VSIRNAS
WHEAT
topic FIJIVIRUS
MRCV
PIRNAS
PLANTHOPPER
RNA SILENCING
SRNAS
VSIRNAS
WHEAT
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Plant reoviruses are able to multiply in gramineae plants and delphacid vectors encountering different defense strategies with unique features. This study aims to comparatively assess alterations of small RNA (sRNA) populations in both hosts upon virus infection. For this purpose, we characterized the sRNA profiles of wheat and planthopper vectors infected by Mal de Río Cuarto virus (MRCV, Fijivirus, Reoviridae) and quantified virus genome segments by quantitative reverse transcription PCR We provide evidence that plant and insect silencing machineries differentially recognize the viral genome, thus giving rise to distinct profiles of virus-derived small interfering RNAs (vsiRNAs). In plants, most of the virus genome segments were targeted preferentially within their upstream sequences and vsiRNAs mapped with higher density to the smaller genome segments than to the medium or larger ones. This tendency, however, was not observed in insects. In both hosts, vsiRNAs were equally derived from sense and antisense RNA strands and the differences in vsiRNAs accumulation did not correlate with mRNAs accumulation. We also established that the piwi-interacting RNA (piRNA) pathway was active in the delphacid vector but, contrary to what is observed in virus-infected mosquitoes, virus-specific piRNAs were not detected. This work contributes to the understanding of the silencing response in insect and plant hosts.
Fil: de Haro, Luis Alejandro. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dumón, Analía Delina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mattio, Maria Fernanda. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Argüello Caro, Evangelina Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Llauger, Gabriela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zavallo, Diego. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Blanc, Hervé. Instituto Pasteur; Francia
Fil: Mongelli, Vanesa Claudia. Instituto Pasteur; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Truol, Graciela Ana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: Saleh, María Carla. Instituto Pasteur; Francia
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Plant reoviruses are able to multiply in gramineae plants and delphacid vectors encountering different defense strategies with unique features. This study aims to comparatively assess alterations of small RNA (sRNA) populations in both hosts upon virus infection. For this purpose, we characterized the sRNA profiles of wheat and planthopper vectors infected by Mal de Río Cuarto virus (MRCV, Fijivirus, Reoviridae) and quantified virus genome segments by quantitative reverse transcription PCR We provide evidence that plant and insect silencing machineries differentially recognize the viral genome, thus giving rise to distinct profiles of virus-derived small interfering RNAs (vsiRNAs). In plants, most of the virus genome segments were targeted preferentially within their upstream sequences and vsiRNAs mapped with higher density to the smaller genome segments than to the medium or larger ones. This tendency, however, was not observed in insects. In both hosts, vsiRNAs were equally derived from sense and antisense RNA strands and the differences in vsiRNAs accumulation did not correlate with mRNAs accumulation. We also established that the piwi-interacting RNA (piRNA) pathway was active in the delphacid vector but, contrary to what is observed in virus-infected mosquitoes, virus-specific piRNAs were not detected. This work contributes to the understanding of the silencing response in insect and plant hosts.
publishDate 2017
dc.date.none.fl_str_mv 2017-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/72863
de Haro, Luis Alejandro; Dumón, Analía Delina; Mattio, Maria Fernanda; Argüello Caro, Evangelina Beatriz; Llauger, Gabriela; et al.; Mal de Río Cuarto virus infection triggers the production of distinctive viral-derived siRNA profiles in wheat and its planthopper vector; Frontiers Research Foundation; Frontiers in Plant Science; 8; 5-2017; 1-11
1664-462X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/72863
identifier_str_mv de Haro, Luis Alejandro; Dumón, Analía Delina; Mattio, Maria Fernanda; Argüello Caro, Evangelina Beatriz; Llauger, Gabriela; et al.; Mal de Río Cuarto virus infection triggers the production of distinctive viral-derived siRNA profiles in wheat and its planthopper vector; Frontiers Research Foundation; Frontiers in Plant Science; 8; 5-2017; 1-11
1664-462X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2017.00766
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fpls.2017.00766/full
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Research Foundation
publisher.none.fl_str_mv Frontiers Research Foundation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.885934

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