High viral load in the planthopper vector Delphacodes kuscheli (Hemiptera: Delphacidae) is associated with successful transmission of Mal de Río Cuarto virus

Autores
Argüello Caro, Evangelina Beatriz; Maroniche, Guillermo Andrés; Dumón, Analía Delina; Sagadín, Mónica B.; del Vas, Mariana; Truol, Graciela Ana
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Delphacodes kuscheli Fennah (Hemiptera: Delphacidae) is the main natural vector of Mal de Rio Cuarto virus (family Reoviridae, genus Fijivirus, MRCV), which infects different gramineae and causes the most important maize (Zea mays L.) disease in Argentina. MRCV—vector interactions usually are studied using different winter cereals as hosts. Under experimental conditions, <50% of D. kuscheli planthoppers fed on a MRCV-infected plant can transmit the virus to wheat (Triticum aestivum L. ). This fact is influenced by insect development stage at acquisition and the latency period. This work describes the relation between transmission efficiency and MRCV accumulation in its planthopper vector. First- and third-instar D. kuscheli nymphs were allowed to feed on MRCV-infected plants, and 9 or 17 d after the acquisition access period (AAP), viral load of transmitting and nontransmitting planthoppers was quantified by quantitative polymerase chain reaction. The transmitting planthoppers showed significantly higher viral titers than nontransmitting ones, suggesting that successful transmission is positively associated to viral accumulation in the insect. However, planthoppers of the third-instars group did not transmit the virus 9 d after AAP, even when 46% had similar titers to the transmitting insects of the other treatments. These results indicate that additional factors influence MRCV transmission efficiency when acquisition occurs in older planthoppers. This is the first precise quantitative analysis of MRCV in its main vector species and will definitely contribute to better understand planthopper—Fijivirus interactions and its epidemiological implications.
Fil: Argüello Caro, Evangelina Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Maroniche, Guillermo Andrés. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dumón, Analía Delina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sagadín, Mónica B.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Truol, Graciela Ana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Delphacodes Kuscheli
Mrcv
Viral Transmission
Fijivirus
Persistent Propagative Transmission
Vector Virus Interaction
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/22157
Acceder
id CONICETDig_407e8b8dac722b5887deddbbc10fa19d
oai_identifier_str oai:ri.conicet.gov.ar:11336/22157
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling High viral load in the planthopper vector Delphacodes kuscheli (Hemiptera: Delphacidae) is associated with successful transmission of Mal de Río Cuarto virusArgüello Caro, Evangelina BeatrizMaroniche, Guillermo AndrésDumón, Analía DelinaSagadín, Mónica B.del Vas, MarianaTruol, Graciela AnaDelphacodes KuscheliMrcvViral TransmissionFijivirusPersistent Propagative TransmissionVector Virus Interactionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Delphacodes kuscheli Fennah (Hemiptera: Delphacidae) is the main natural vector of Mal de Rio Cuarto virus (family Reoviridae, genus Fijivirus, MRCV), which infects different gramineae and causes the most important maize (Zea mays L.) disease in Argentina. MRCV—vector interactions usually are studied using different winter cereals as hosts. Under experimental conditions, <50% of D. kuscheli planthoppers fed on a MRCV-infected plant can transmit the virus to wheat (Triticum aestivum L. ). This fact is influenced by insect development stage at acquisition and the latency period. This work describes the relation between transmission efficiency and MRCV accumulation in its planthopper vector. First- and third-instar D. kuscheli nymphs were allowed to feed on MRCV-infected plants, and 9 or 17 d after the acquisition access period (AAP), viral load of transmitting and nontransmitting planthoppers was quantified by quantitative polymerase chain reaction. The transmitting planthoppers showed significantly higher viral titers than nontransmitting ones, suggesting that successful transmission is positively associated to viral accumulation in the insect. However, planthoppers of the third-instars group did not transmit the virus 9 d after AAP, even when 46% had similar titers to the transmitting insects of the other treatments. These results indicate that additional factors influence MRCV transmission efficiency when acquisition occurs in older planthoppers. This is the first precise quantitative analysis of MRCV in its main vector species and will definitely contribute to better understand planthopper—Fijivirus interactions and its epidemiological implications.Fil: Argüello Caro, Evangelina Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Maroniche, Guillermo Andrés. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Dumón, Analía Delina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sagadín, Mónica B.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Truol, Graciela Ana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaEntomological Society of America2013-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/22157Argüello Caro, Evangelina Beatriz; Maroniche, Guillermo Andrés; Dumón, Analía Delina; Sagadín, Mónica B.; del Vas, Mariana; et al.; High viral load in the planthopper vector Delphacodes kuscheli (Hemiptera: Delphacidae) is associated with successful transmission of Mal de Río Cuarto virus; Entomological Society of America; Annals of the Entomologycal Society of America; 106; 1; 1-2013; 93-990013-8746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.bioone.org/doi/abstract/10.1603/AN12076info:eu-repo/semantics/altIdentifier/doi/10.1603/AN12076info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/aesa/article-abstract/106/1/93/2758511/High-Viral-Load-in-the-Planthopper-Vector?redirectedFrom=fulltextinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:15:15Zoai:ri.conicet.gov.ar:11336/22157instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:15:15.38CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv High viral load in the planthopper vector Delphacodes kuscheli (Hemiptera: Delphacidae) is associated with successful transmission of Mal de Río Cuarto virus
title High viral load in the planthopper vector Delphacodes kuscheli (Hemiptera: Delphacidae) is associated with successful transmission of Mal de Río Cuarto virus
spellingShingle High viral load in the planthopper vector Delphacodes kuscheli (Hemiptera: Delphacidae) is associated with successful transmission of Mal de Río Cuarto virus
Argüello Caro, Evangelina Beatriz
Delphacodes Kuscheli
Mrcv
Viral Transmission
Fijivirus
Persistent Propagative Transmission
Vector Virus Interaction
title_short High viral load in the planthopper vector Delphacodes kuscheli (Hemiptera: Delphacidae) is associated with successful transmission of Mal de Río Cuarto virus
title_full High viral load in the planthopper vector Delphacodes kuscheli (Hemiptera: Delphacidae) is associated with successful transmission of Mal de Río Cuarto virus
title_fullStr High viral load in the planthopper vector Delphacodes kuscheli (Hemiptera: Delphacidae) is associated with successful transmission of Mal de Río Cuarto virus
title_full_unstemmed High viral load in the planthopper vector Delphacodes kuscheli (Hemiptera: Delphacidae) is associated with successful transmission of Mal de Río Cuarto virus
title_sort High viral load in the planthopper vector Delphacodes kuscheli (Hemiptera: Delphacidae) is associated with successful transmission of Mal de Río Cuarto virus
dc.creator.none.fl_str_mv Argüello Caro, Evangelina Beatriz
Maroniche, Guillermo Andrés
Dumón, Analía Delina
Sagadín, Mónica B.
del Vas, Mariana
Truol, Graciela Ana
author Argüello Caro, Evangelina Beatriz
author_facet Argüello Caro, Evangelina Beatriz
Maroniche, Guillermo Andrés
Dumón, Analía Delina
Sagadín, Mónica B.
del Vas, Mariana
Truol, Graciela Ana
author_role author
author2 Maroniche, Guillermo Andrés
Dumón, Analía Delina
Sagadín, Mónica B.
del Vas, Mariana
Truol, Graciela Ana
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Delphacodes Kuscheli
Mrcv
Viral Transmission
Fijivirus
Persistent Propagative Transmission
Vector Virus Interaction
topic Delphacodes Kuscheli
Mrcv
Viral Transmission
Fijivirus
Persistent Propagative Transmission
Vector Virus Interaction
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Delphacodes kuscheli Fennah (Hemiptera: Delphacidae) is the main natural vector of Mal de Rio Cuarto virus (family Reoviridae, genus Fijivirus, MRCV), which infects different gramineae and causes the most important maize (Zea mays L.) disease in Argentina. MRCV—vector interactions usually are studied using different winter cereals as hosts. Under experimental conditions, <50% of D. kuscheli planthoppers fed on a MRCV-infected plant can transmit the virus to wheat (Triticum aestivum L. ). This fact is influenced by insect development stage at acquisition and the latency period. This work describes the relation between transmission efficiency and MRCV accumulation in its planthopper vector. First- and third-instar D. kuscheli nymphs were allowed to feed on MRCV-infected plants, and 9 or 17 d after the acquisition access period (AAP), viral load of transmitting and nontransmitting planthoppers was quantified by quantitative polymerase chain reaction. The transmitting planthoppers showed significantly higher viral titers than nontransmitting ones, suggesting that successful transmission is positively associated to viral accumulation in the insect. However, planthoppers of the third-instars group did not transmit the virus 9 d after AAP, even when 46% had similar titers to the transmitting insects of the other treatments. These results indicate that additional factors influence MRCV transmission efficiency when acquisition occurs in older planthoppers. This is the first precise quantitative analysis of MRCV in its main vector species and will definitely contribute to better understand planthopper—Fijivirus interactions and its epidemiological implications.
Fil: Argüello Caro, Evangelina Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Maroniche, Guillermo Andrés. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dumón, Analía Delina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sagadín, Mónica B.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Truol, Graciela Ana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Delphacodes kuscheli Fennah (Hemiptera: Delphacidae) is the main natural vector of Mal de Rio Cuarto virus (family Reoviridae, genus Fijivirus, MRCV), which infects different gramineae and causes the most important maize (Zea mays L.) disease in Argentina. MRCV—vector interactions usually are studied using different winter cereals as hosts. Under experimental conditions, <50% of D. kuscheli planthoppers fed on a MRCV-infected plant can transmit the virus to wheat (Triticum aestivum L. ). This fact is influenced by insect development stage at acquisition and the latency period. This work describes the relation between transmission efficiency and MRCV accumulation in its planthopper vector. First- and third-instar D. kuscheli nymphs were allowed to feed on MRCV-infected plants, and 9 or 17 d after the acquisition access period (AAP), viral load of transmitting and nontransmitting planthoppers was quantified by quantitative polymerase chain reaction. The transmitting planthoppers showed significantly higher viral titers than nontransmitting ones, suggesting that successful transmission is positively associated to viral accumulation in the insect. However, planthoppers of the third-instars group did not transmit the virus 9 d after AAP, even when 46% had similar titers to the transmitting insects of the other treatments. These results indicate that additional factors influence MRCV transmission efficiency when acquisition occurs in older planthoppers. This is the first precise quantitative analysis of MRCV in its main vector species and will definitely contribute to better understand planthopper—Fijivirus interactions and its epidemiological implications.
publishDate 2013
dc.date.none.fl_str_mv 2013-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/22157
Argüello Caro, Evangelina Beatriz; Maroniche, Guillermo Andrés; Dumón, Analía Delina; Sagadín, Mónica B.; del Vas, Mariana; et al.; High viral load in the planthopper vector Delphacodes kuscheli (Hemiptera: Delphacidae) is associated with successful transmission of Mal de Río Cuarto virus; Entomological Society of America; Annals of the Entomologycal Society of America; 106; 1; 1-2013; 93-99
0013-8746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/22157
identifier_str_mv Argüello Caro, Evangelina Beatriz; Maroniche, Guillermo Andrés; Dumón, Analía Delina; Sagadín, Mónica B.; del Vas, Mariana; et al.; High viral load in the planthopper vector Delphacodes kuscheli (Hemiptera: Delphacidae) is associated with successful transmission of Mal de Río Cuarto virus; Entomological Society of America; Annals of the Entomologycal Society of America; 106; 1; 1-2013; 93-99
0013-8746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.bioone.org/doi/abstract/10.1603/AN12076
info:eu-repo/semantics/altIdentifier/doi/10.1603/AN12076
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/aesa/article-abstract/106/1/93/2758511/High-Viral-Load-in-the-Planthopper-Vector?redirectedFrom=fulltext
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
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application/pdf
dc.publisher.none.fl_str_mv Entomological Society of America
publisher.none.fl_str_mv Entomological Society of America
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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