Triatoma brasiliensis (Neiva, 1911): Food sources and diversity of Trypanosoma cruzi in wild and artificial environments of the semiarid region of Ceará, Northeastern Brazil

Autores
Mendonça Bezerra, Claudia; Barbosa, Silvia Ermelinda; de Cássia Moreira de Souza, Rita; Barezani, Carla Patrícia; Gurtler, Ricardo Esteban; Novaes Ramos, Alberto; Diotaiuti, Liléia
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Knowledge of triatomine food sources in different ecotopes enables the estimation of T. cruzi transmission risk in diverse environments, as well as its dynamics of dispersion and ecological niche. For Triatoma brasiliensis in the Caatinga, in the northeast of Brazil, seasonal differences influence feeding eclecticism and rates of T. cruzi infection. The objective of the present study was to monitor food sources and to characterize the populations of T. cruzi associated with T. brasiliensis in wild and domestic environments in the Caatinga of northeast Brazil. Methods: A cross-sectional study based on a search for triatomines in wild and domestic environments, was undertaken at five different time periods from 2009 to 2015. Insects from 2015 were used for identification of food sources. Two universal primers, based on the conserved regions of the 12S rRNA locus, were used to amplify fragments of 215 bp. The content of the intestinal tract of triatomines was identified by a comparison between the sequences obtained and those deposited in the GenBank database, using BLAST. In triatomines with parasitological diagnosis of infection by trypanosomatids, xenoculture was performed for the isolation and characterization of strains, using cox2, the amplification of the SL-IL mini-exon intergenic spacer and the polymorphism of the D7 divergent domain of the gene 24αrDNA-LSU. Results: Food sources were identified in 76.3% (213/279) T. brasiliensis specimens sampled in 2015. The most frequent sources in a total of 20 vertebrate species were: rodents (58%, 123/213), ruminants (30%, 64/213) and cats (6%, 12/213). A total of 49% (44/89) of the samples of T. cruzi isolated in the period from 2009 to 2015 were characterized: TcII (43%, 19/44), TcI (41%, 18/44) and TcIII (16%, 7/44). Conclusions: The feeding eclecticism of T. brasiliensis shows its importance in maintaining the transmission dynamics of T. cruzi, with evidence of intense circulation between anthropic and wild environments. Attention should be placed on the association among T. brasiliensis, rodents and ruminants, in addition to the presence of TcIII in the study region.
Fil: Mendonça Bezerra, Claudia. Universidade Federal do Ceara; Brasil. Estado do Ceará. Secretaria de Saúde; Brasil
Fil: Barbosa, Silvia Ermelinda. Fundación Oswaldo Cruz; Brasil
Fil: de Cássia Moreira de Souza, Rita. Fundación Oswaldo Cruz; Brasil
Fil: Barezani, Carla Patrícia. Fundación Oswaldo Cruz; Brasil
Fil: Gurtler, Ricardo Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
Fil: Novaes Ramos, Alberto. Universidade Federal do Ceara; Brasil
Fil: Diotaiuti, Liléia. Fundación Oswaldo Cruz; Brasil
Materia
BRAZIL
CAATINGA
CHAGAS DISEASE
DISCRETE TYPING UNIT
EATING BEHAVIOR
TRIATOMA BRASILIENSIS
TRYPANOSOMA CRUZI
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/163551

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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Triatoma brasiliensis (Neiva, 1911): Food sources and diversity of Trypanosoma cruzi in wild and artificial environments of the semiarid region of Ceará, Northeastern BrazilMendonça Bezerra, ClaudiaBarbosa, Silvia Ermelindade Cássia Moreira de Souza, RitaBarezani, Carla PatríciaGurtler, Ricardo EstebanNovaes Ramos, AlbertoDiotaiuti, LiléiaBRAZILCAATINGACHAGAS DISEASEDISCRETE TYPING UNITEATING BEHAVIORTRIATOMA BRASILIENSISTRYPANOSOMA CRUZIhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: Knowledge of triatomine food sources in different ecotopes enables the estimation of T. cruzi transmission risk in diverse environments, as well as its dynamics of dispersion and ecological niche. For Triatoma brasiliensis in the Caatinga, in the northeast of Brazil, seasonal differences influence feeding eclecticism and rates of T. cruzi infection. The objective of the present study was to monitor food sources and to characterize the populations of T. cruzi associated with T. brasiliensis in wild and domestic environments in the Caatinga of northeast Brazil. Methods: A cross-sectional study based on a search for triatomines in wild and domestic environments, was undertaken at five different time periods from 2009 to 2015. Insects from 2015 were used for identification of food sources. Two universal primers, based on the conserved regions of the 12S rRNA locus, were used to amplify fragments of 215 bp. The content of the intestinal tract of triatomines was identified by a comparison between the sequences obtained and those deposited in the GenBank database, using BLAST. In triatomines with parasitological diagnosis of infection by trypanosomatids, xenoculture was performed for the isolation and characterization of strains, using cox2, the amplification of the SL-IL mini-exon intergenic spacer and the polymorphism of the D7 divergent domain of the gene 24αrDNA-LSU. Results: Food sources were identified in 76.3% (213/279) T. brasiliensis specimens sampled in 2015. The most frequent sources in a total of 20 vertebrate species were: rodents (58%, 123/213), ruminants (30%, 64/213) and cats (6%, 12/213). A total of 49% (44/89) of the samples of T. cruzi isolated in the period from 2009 to 2015 were characterized: TcII (43%, 19/44), TcI (41%, 18/44) and TcIII (16%, 7/44). Conclusions: The feeding eclecticism of T. brasiliensis shows its importance in maintaining the transmission dynamics of T. cruzi, with evidence of intense circulation between anthropic and wild environments. Attention should be placed on the association among T. brasiliensis, rodents and ruminants, in addition to the presence of TcIII in the study region.Fil: Mendonça Bezerra, Claudia. Universidade Federal do Ceara; Brasil. Estado do Ceará. Secretaria de Saúde; BrasilFil: Barbosa, Silvia Ermelinda. Fundación Oswaldo Cruz; BrasilFil: de Cássia Moreira de Souza, Rita. Fundación Oswaldo Cruz; BrasilFil: Barezani, Carla Patrícia. Fundación Oswaldo Cruz; BrasilFil: Gurtler, Ricardo Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Novaes Ramos, Alberto. Universidade Federal do Ceara; BrasilFil: Diotaiuti, Liléia. Fundación Oswaldo Cruz; BrasilBioMed Central2019-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/163551Mendonça Bezerra, Claudia; Barbosa, Silvia Ermelinda; de Cássia Moreira de Souza, Rita; Barezani, Carla Patrícia; Gurtler, Ricardo Esteban; et al.; Triatoma brasiliensis (Neiva, 1911): Food sources and diversity of Trypanosoma cruzi in wild and artificial environments of the semiarid region of Ceará, Northeastern Brazil; BioMed Central; Parasites and Vectors; 11; 642; 6-2019; 1-141756-3305CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-018-3235-4info:eu-repo/semantics/altIdentifier/doi/10.1186/s13071-018-3235-4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:39:03Zoai:ri.conicet.gov.ar:11336/163551instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:39:03.909CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Triatoma brasiliensis (Neiva, 1911): Food sources and diversity of Trypanosoma cruzi in wild and artificial environments of the semiarid region of Ceará, Northeastern Brazil
title Triatoma brasiliensis (Neiva, 1911): Food sources and diversity of Trypanosoma cruzi in wild and artificial environments of the semiarid region of Ceará, Northeastern Brazil
spellingShingle Triatoma brasiliensis (Neiva, 1911): Food sources and diversity of Trypanosoma cruzi in wild and artificial environments of the semiarid region of Ceará, Northeastern Brazil
Mendonça Bezerra, Claudia
BRAZIL
CAATINGA
CHAGAS DISEASE
DISCRETE TYPING UNIT
EATING BEHAVIOR
TRIATOMA BRASILIENSIS
TRYPANOSOMA CRUZI
title_short Triatoma brasiliensis (Neiva, 1911): Food sources and diversity of Trypanosoma cruzi in wild and artificial environments of the semiarid region of Ceará, Northeastern Brazil
title_full Triatoma brasiliensis (Neiva, 1911): Food sources and diversity of Trypanosoma cruzi in wild and artificial environments of the semiarid region of Ceará, Northeastern Brazil
title_fullStr Triatoma brasiliensis (Neiva, 1911): Food sources and diversity of Trypanosoma cruzi in wild and artificial environments of the semiarid region of Ceará, Northeastern Brazil
title_full_unstemmed Triatoma brasiliensis (Neiva, 1911): Food sources and diversity of Trypanosoma cruzi in wild and artificial environments of the semiarid region of Ceará, Northeastern Brazil
title_sort Triatoma brasiliensis (Neiva, 1911): Food sources and diversity of Trypanosoma cruzi in wild and artificial environments of the semiarid region of Ceará, Northeastern Brazil
dc.creator.none.fl_str_mv Mendonça Bezerra, Claudia
Barbosa, Silvia Ermelinda
de Cássia Moreira de Souza, Rita
Barezani, Carla Patrícia
Gurtler, Ricardo Esteban
Novaes Ramos, Alberto
Diotaiuti, Liléia
author Mendonça Bezerra, Claudia
author_facet Mendonça Bezerra, Claudia
Barbosa, Silvia Ermelinda
de Cássia Moreira de Souza, Rita
Barezani, Carla Patrícia
Gurtler, Ricardo Esteban
Novaes Ramos, Alberto
Diotaiuti, Liléia
author_role author
author2 Barbosa, Silvia Ermelinda
de Cássia Moreira de Souza, Rita
Barezani, Carla Patrícia
Gurtler, Ricardo Esteban
Novaes Ramos, Alberto
Diotaiuti, Liléia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv BRAZIL
CAATINGA
CHAGAS DISEASE
DISCRETE TYPING UNIT
EATING BEHAVIOR
TRIATOMA BRASILIENSIS
TRYPANOSOMA CRUZI
topic BRAZIL
CAATINGA
CHAGAS DISEASE
DISCRETE TYPING UNIT
EATING BEHAVIOR
TRIATOMA BRASILIENSIS
TRYPANOSOMA CRUZI
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: Knowledge of triatomine food sources in different ecotopes enables the estimation of T. cruzi transmission risk in diverse environments, as well as its dynamics of dispersion and ecological niche. For Triatoma brasiliensis in the Caatinga, in the northeast of Brazil, seasonal differences influence feeding eclecticism and rates of T. cruzi infection. The objective of the present study was to monitor food sources and to characterize the populations of T. cruzi associated with T. brasiliensis in wild and domestic environments in the Caatinga of northeast Brazil. Methods: A cross-sectional study based on a search for triatomines in wild and domestic environments, was undertaken at five different time periods from 2009 to 2015. Insects from 2015 were used for identification of food sources. Two universal primers, based on the conserved regions of the 12S rRNA locus, were used to amplify fragments of 215 bp. The content of the intestinal tract of triatomines was identified by a comparison between the sequences obtained and those deposited in the GenBank database, using BLAST. In triatomines with parasitological diagnosis of infection by trypanosomatids, xenoculture was performed for the isolation and characterization of strains, using cox2, the amplification of the SL-IL mini-exon intergenic spacer and the polymorphism of the D7 divergent domain of the gene 24αrDNA-LSU. Results: Food sources were identified in 76.3% (213/279) T. brasiliensis specimens sampled in 2015. The most frequent sources in a total of 20 vertebrate species were: rodents (58%, 123/213), ruminants (30%, 64/213) and cats (6%, 12/213). A total of 49% (44/89) of the samples of T. cruzi isolated in the period from 2009 to 2015 were characterized: TcII (43%, 19/44), TcI (41%, 18/44) and TcIII (16%, 7/44). Conclusions: The feeding eclecticism of T. brasiliensis shows its importance in maintaining the transmission dynamics of T. cruzi, with evidence of intense circulation between anthropic and wild environments. Attention should be placed on the association among T. brasiliensis, rodents and ruminants, in addition to the presence of TcIII in the study region.
Fil: Mendonça Bezerra, Claudia. Universidade Federal do Ceara; Brasil. Estado do Ceará. Secretaria de Saúde; Brasil
Fil: Barbosa, Silvia Ermelinda. Fundación Oswaldo Cruz; Brasil
Fil: de Cássia Moreira de Souza, Rita. Fundación Oswaldo Cruz; Brasil
Fil: Barezani, Carla Patrícia. Fundación Oswaldo Cruz; Brasil
Fil: Gurtler, Ricardo Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
Fil: Novaes Ramos, Alberto. Universidade Federal do Ceara; Brasil
Fil: Diotaiuti, Liléia. Fundación Oswaldo Cruz; Brasil
description Background: Knowledge of triatomine food sources in different ecotopes enables the estimation of T. cruzi transmission risk in diverse environments, as well as its dynamics of dispersion and ecological niche. For Triatoma brasiliensis in the Caatinga, in the northeast of Brazil, seasonal differences influence feeding eclecticism and rates of T. cruzi infection. The objective of the present study was to monitor food sources and to characterize the populations of T. cruzi associated with T. brasiliensis in wild and domestic environments in the Caatinga of northeast Brazil. Methods: A cross-sectional study based on a search for triatomines in wild and domestic environments, was undertaken at five different time periods from 2009 to 2015. Insects from 2015 were used for identification of food sources. Two universal primers, based on the conserved regions of the 12S rRNA locus, were used to amplify fragments of 215 bp. The content of the intestinal tract of triatomines was identified by a comparison between the sequences obtained and those deposited in the GenBank database, using BLAST. In triatomines with parasitological diagnosis of infection by trypanosomatids, xenoculture was performed for the isolation and characterization of strains, using cox2, the amplification of the SL-IL mini-exon intergenic spacer and the polymorphism of the D7 divergent domain of the gene 24αrDNA-LSU. Results: Food sources were identified in 76.3% (213/279) T. brasiliensis specimens sampled in 2015. The most frequent sources in a total of 20 vertebrate species were: rodents (58%, 123/213), ruminants (30%, 64/213) and cats (6%, 12/213). A total of 49% (44/89) of the samples of T. cruzi isolated in the period from 2009 to 2015 were characterized: TcII (43%, 19/44), TcI (41%, 18/44) and TcIII (16%, 7/44). Conclusions: The feeding eclecticism of T. brasiliensis shows its importance in maintaining the transmission dynamics of T. cruzi, with evidence of intense circulation between anthropic and wild environments. Attention should be placed on the association among T. brasiliensis, rodents and ruminants, in addition to the presence of TcIII in the study region.
publishDate 2019
dc.date.none.fl_str_mv 2019-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/163551
Mendonça Bezerra, Claudia; Barbosa, Silvia Ermelinda; de Cássia Moreira de Souza, Rita; Barezani, Carla Patrícia; Gurtler, Ricardo Esteban; et al.; Triatoma brasiliensis (Neiva, 1911): Food sources and diversity of Trypanosoma cruzi in wild and artificial environments of the semiarid region of Ceará, Northeastern Brazil; BioMed Central; Parasites and Vectors; 11; 642; 6-2019; 1-14
1756-3305
CONICET Digital
CONICET
url http://hdl.handle.net/11336/163551
identifier_str_mv Mendonça Bezerra, Claudia; Barbosa, Silvia Ermelinda; de Cássia Moreira de Souza, Rita; Barezani, Carla Patrícia; Gurtler, Ricardo Esteban; et al.; Triatoma brasiliensis (Neiva, 1911): Food sources and diversity of Trypanosoma cruzi in wild and artificial environments of the semiarid region of Ceará, Northeastern Brazil; BioMed Central; Parasites and Vectors; 11; 642; 6-2019; 1-14
1756-3305
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1186/s13071-018-3235-4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
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dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
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