Development of an electroanalytical method for the quantification of zearalenone (ZEA) in maize samples

Autores
Ramírez, Eduardo Alejandro; Molina, Patricia Gabriela; Zón, María Alicia; Fernández, Héctor
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The application of electroanalytical techniques to detect and quantify zearalenone (ZEA) mycotoxin that frequently contaminates maize and foodstuff is studied in this work. Rice and maize grains were inoculated with Fusarium fungus to obtain ZEA in artificially infected samples. The electro-oxidation of ZEA adsorbed on the surface of glassy carbon (GC) electrodes in 20% acetonitrile (ACN) + 80% 1 M HClO4 (aqueous solution) reaction medium was studied by using square-wave voltammetry (SWV). Studies were conducted to find the most favorable accumulation potential (Eacc) and accumulation time (t acc) to perform the ZEA preconcentration on the electrode surface. It was found that Eacc was any value in the range from 0.00-0.90 V and the best tacc was 120 s, respectively, for ZEA separated from extracting solution by TLC (ZEATLC) while Eacc = 0.90 V corresponded to ZEA in non separated matrix solution (ZEAmatrix). The ZEA quantitative determination was performed by SWV combined with the standard addition method. Linear plots were obtained from the net peak current (I p, n) vs c*ZEA in the concentration range from 20 to 3184 ppb. Detection limit of 30 ppb at a signal to noise ratio of 3:1 was obtained. On the other hand, recovery experiments were performed on uncontaminated maize samples spiked with ZEA.
Fil: Ramírez, Eduardo Alejandro. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Molina, Patricia Gabriela. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Zón, María Alicia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Fernández, Héctor. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Materia
ADSORPTIVE ACCUMULATION
MAIZE
MYCOTOXINS
SQUARE-WAVE VOLTAMMETRY
ZEARALENONA (ZEA)
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/115570
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/115570
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development of an electroanalytical method for the quantification of zearalenone (ZEA) in maize samplesRamírez, Eduardo AlejandroMolina, Patricia GabrielaZón, María AliciaFernández, HéctorADSORPTIVE ACCUMULATIONMAIZEMYCOTOXINSSQUARE-WAVE VOLTAMMETRYZEARALENONA (ZEA)https://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The application of electroanalytical techniques to detect and quantify zearalenone (ZEA) mycotoxin that frequently contaminates maize and foodstuff is studied in this work. Rice and maize grains were inoculated with Fusarium fungus to obtain ZEA in artificially infected samples. The electro-oxidation of ZEA adsorbed on the surface of glassy carbon (GC) electrodes in 20% acetonitrile (ACN) + 80% 1 M HClO4 (aqueous solution) reaction medium was studied by using square-wave voltammetry (SWV). Studies were conducted to find the most favorable accumulation potential (Eacc) and accumulation time (t acc) to perform the ZEA preconcentration on the electrode surface. It was found that Eacc was any value in the range from 0.00-0.90 V and the best tacc was 120 s, respectively, for ZEA separated from extracting solution by TLC (ZEATLC) while Eacc = 0.90 V corresponded to ZEA in non separated matrix solution (ZEAmatrix). The ZEA quantitative determination was performed by SWV combined with the standard addition method. Linear plots were obtained from the net peak current (I p, n) vs c*ZEA in the concentration range from 20 to 3184 ppb. Detection limit of 30 ppb at a signal to noise ratio of 3:1 was obtained. On the other hand, recovery experiments were performed on uncontaminated maize samples spiked with ZEA.Fil: Ramírez, Eduardo Alejandro. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Molina, Patricia Gabriela. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Zón, María Alicia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Fernández, Héctor. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaWiley VCH Verlag2005-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/115570Ramírez, Eduardo Alejandro; Molina, Patricia Gabriela; Zón, María Alicia; Fernández, Héctor; Development of an electroanalytical method for the quantification of zearalenone (ZEA) in maize samples; Wiley VCH Verlag; Electroanalysis; 17; 18; 9-2005; 1635-16401040-03971521-4109CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/elan.200403193info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/elan.200403193info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:05Zoai:ri.conicet.gov.ar:11336/115570instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:05.919CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development of an electroanalytical method for the quantification of zearalenone (ZEA) in maize samples
title Development of an electroanalytical method for the quantification of zearalenone (ZEA) in maize samples
spellingShingle Development of an electroanalytical method for the quantification of zearalenone (ZEA) in maize samples
Ramírez, Eduardo Alejandro
ADSORPTIVE ACCUMULATION
MAIZE
MYCOTOXINS
SQUARE-WAVE VOLTAMMETRY
ZEARALENONA (ZEA)
title_short Development of an electroanalytical method for the quantification of zearalenone (ZEA) in maize samples
title_full Development of an electroanalytical method for the quantification of zearalenone (ZEA) in maize samples
title_fullStr Development of an electroanalytical method for the quantification of zearalenone (ZEA) in maize samples
title_full_unstemmed Development of an electroanalytical method for the quantification of zearalenone (ZEA) in maize samples
title_sort Development of an electroanalytical method for the quantification of zearalenone (ZEA) in maize samples
dc.creator.none.fl_str_mv Ramírez, Eduardo Alejandro
Molina, Patricia Gabriela
Zón, María Alicia
Fernández, Héctor
author Ramírez, Eduardo Alejandro
author_facet Ramírez, Eduardo Alejandro
Molina, Patricia Gabriela
Zón, María Alicia
Fernández, Héctor
author_role author
author2 Molina, Patricia Gabriela
Zón, María Alicia
Fernández, Héctor
author2_role author
author
author
dc.subject.none.fl_str_mv ADSORPTIVE ACCUMULATION
MAIZE
MYCOTOXINS
SQUARE-WAVE VOLTAMMETRY
ZEARALENONA (ZEA)
topic ADSORPTIVE ACCUMULATION
MAIZE
MYCOTOXINS
SQUARE-WAVE VOLTAMMETRY
ZEARALENONA (ZEA)
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The application of electroanalytical techniques to detect and quantify zearalenone (ZEA) mycotoxin that frequently contaminates maize and foodstuff is studied in this work. Rice and maize grains were inoculated with Fusarium fungus to obtain ZEA in artificially infected samples. The electro-oxidation of ZEA adsorbed on the surface of glassy carbon (GC) electrodes in 20% acetonitrile (ACN) + 80% 1 M HClO4 (aqueous solution) reaction medium was studied by using square-wave voltammetry (SWV). Studies were conducted to find the most favorable accumulation potential (Eacc) and accumulation time (t acc) to perform the ZEA preconcentration on the electrode surface. It was found that Eacc was any value in the range from 0.00-0.90 V and the best tacc was 120 s, respectively, for ZEA separated from extracting solution by TLC (ZEATLC) while Eacc = 0.90 V corresponded to ZEA in non separated matrix solution (ZEAmatrix). The ZEA quantitative determination was performed by SWV combined with the standard addition method. Linear plots were obtained from the net peak current (I p, n) vs c*ZEA in the concentration range from 20 to 3184 ppb. Detection limit of 30 ppb at a signal to noise ratio of 3:1 was obtained. On the other hand, recovery experiments were performed on uncontaminated maize samples spiked with ZEA.
Fil: Ramírez, Eduardo Alejandro. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Molina, Patricia Gabriela. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Zón, María Alicia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Fernández, Héctor. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
description The application of electroanalytical techniques to detect and quantify zearalenone (ZEA) mycotoxin that frequently contaminates maize and foodstuff is studied in this work. Rice and maize grains were inoculated with Fusarium fungus to obtain ZEA in artificially infected samples. The electro-oxidation of ZEA adsorbed on the surface of glassy carbon (GC) electrodes in 20% acetonitrile (ACN) + 80% 1 M HClO4 (aqueous solution) reaction medium was studied by using square-wave voltammetry (SWV). Studies were conducted to find the most favorable accumulation potential (Eacc) and accumulation time (t acc) to perform the ZEA preconcentration on the electrode surface. It was found that Eacc was any value in the range from 0.00-0.90 V and the best tacc was 120 s, respectively, for ZEA separated from extracting solution by TLC (ZEATLC) while Eacc = 0.90 V corresponded to ZEA in non separated matrix solution (ZEAmatrix). The ZEA quantitative determination was performed by SWV combined with the standard addition method. Linear plots were obtained from the net peak current (I p, n) vs c*ZEA in the concentration range from 20 to 3184 ppb. Detection limit of 30 ppb at a signal to noise ratio of 3:1 was obtained. On the other hand, recovery experiments were performed on uncontaminated maize samples spiked with ZEA.
publishDate 2005
dc.date.none.fl_str_mv 2005-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/115570
Ramírez, Eduardo Alejandro; Molina, Patricia Gabriela; Zón, María Alicia; Fernández, Héctor; Development of an electroanalytical method for the quantification of zearalenone (ZEA) in maize samples; Wiley VCH Verlag; Electroanalysis; 17; 18; 9-2005; 1635-1640
1040-0397
1521-4109
CONICET Digital
CONICET
url http://hdl.handle.net/11336/115570
identifier_str_mv Ramírez, Eduardo Alejandro; Molina, Patricia Gabriela; Zón, María Alicia; Fernández, Héctor; Development of an electroanalytical method for the quantification of zearalenone (ZEA) in maize samples; Wiley VCH Verlag; Electroanalysis; 17; 18; 9-2005; 1635-1640
1040-0397
1521-4109
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/elan.200403193
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/elan.200403193
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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