Determination of cercosporin (CER) phytotoxin isolated from infected peanut leaves by using adsorptive stripping square wave voltammetry

Autores
Marchiando, Nancy Cristina; Zon, María Alicia; Fernandez, Hector
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The adsorptive accumulation of cercosporin (CER) phytotoxin on glassy carbon (GC) electrodes from 1 M HClO4 aqueous solutions is used to detect and quantify the phytotoxin isolated from infected leaf tissue collected from field-grown peanut in five locations in southern regions of the Provinces of Cordoba and San Luis, Argentina. The adsorptive stripping square wave voltammetry (ASSWV) is employed to ´ perform the quantitative determination of CER in naturally infected extracts. Thin layer chromatography (TLC) and HPLC were used to verify the presence of CER in extracts. Values of Rf = 0.23 and Rf = 0.15 were determined for CER by TLC when extracts were dissolved in ethyl acetate (EA) and acetone (Ac), respectively, while a retention time of 6.13 min (Ac) was determined by HPLC. The recovery percentage of CER using ASSWV was determined by employing healthy peanut leaf tissue spiked with standard CER. The application of standard addition method was used to determine the recovery percentage as well as the CER contamination level in infected extracts. A recovery percentage of 93.27% was obtained with a relative standard deviation of 21.8% for a solution concentration of 1.04 × 10−6 mol dm−3 (n = 11). The limit of detection for a signal to noise ratio of 3:1 was about 6 ppb and the quantitation limit was about 50 ppb. The pondered relative standard deviation of 9.5% was calculated for the ASSWV method. Results found for CER contamination level in extracts through the electroanalytical method here proposed are in reasonable agreement with those values determined by using HPLC measurements.
Fil: Marchiando, Nancy Cristina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Zon, María Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Fernandez, Hector. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Materia
Cercosporin
Phytotoxins
Peanut
Square wave voltammetry
Adsorptive accumulation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/241839
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/241839
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Determination of cercosporin (CER) phytotoxin isolated from infected peanut leaves by using adsorptive stripping square wave voltammetryMarchiando, Nancy CristinaZon, María AliciaFernandez, HectorCercosporinPhytotoxinsPeanutSquare wave voltammetryAdsorptive accumulationhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The adsorptive accumulation of cercosporin (CER) phytotoxin on glassy carbon (GC) electrodes from 1 M HClO4 aqueous solutions is used to detect and quantify the phytotoxin isolated from infected leaf tissue collected from field-grown peanut in five locations in southern regions of the Provinces of Cordoba and San Luis, Argentina. The adsorptive stripping square wave voltammetry (ASSWV) is employed to ´ perform the quantitative determination of CER in naturally infected extracts. Thin layer chromatography (TLC) and HPLC were used to verify the presence of CER in extracts. Values of Rf = 0.23 and Rf = 0.15 were determined for CER by TLC when extracts were dissolved in ethyl acetate (EA) and acetone (Ac), respectively, while a retention time of 6.13 min (Ac) was determined by HPLC. The recovery percentage of CER using ASSWV was determined by employing healthy peanut leaf tissue spiked with standard CER. The application of standard addition method was used to determine the recovery percentage as well as the CER contamination level in infected extracts. A recovery percentage of 93.27% was obtained with a relative standard deviation of 21.8% for a solution concentration of 1.04 × 10−6 mol dm−3 (n = 11). The limit of detection for a signal to noise ratio of 3:1 was about 6 ppb and the quantitation limit was about 50 ppb. The pondered relative standard deviation of 9.5% was calculated for the ASSWV method. Results found for CER contamination level in extracts through the electroanalytical method here proposed are in reasonable agreement with those values determined by using HPLC measurements.Fil: Marchiando, Nancy Cristina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Zon, María Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Fernandez, Hector. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaElsevier Science2005-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/241839Marchiando, Nancy Cristina; Zon, María Alicia; Fernandez, Hector; Determination of cercosporin (CER) phytotoxin isolated from infected peanut leaves by using adsorptive stripping square wave voltammetry; Elsevier Science; Analytica Chimica Acta; 550; 1-2; 9-2005; 199-2030003-2670CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S000326700501069Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.aca.2005.06.054info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:48:47Zoai:ri.conicet.gov.ar:11336/241839instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:48:47.475CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Determination of cercosporin (CER) phytotoxin isolated from infected peanut leaves by using adsorptive stripping square wave voltammetry
title Determination of cercosporin (CER) phytotoxin isolated from infected peanut leaves by using adsorptive stripping square wave voltammetry
spellingShingle Determination of cercosporin (CER) phytotoxin isolated from infected peanut leaves by using adsorptive stripping square wave voltammetry
Marchiando, Nancy Cristina
Cercosporin
Phytotoxins
Peanut
Square wave voltammetry
Adsorptive accumulation
title_short Determination of cercosporin (CER) phytotoxin isolated from infected peanut leaves by using adsorptive stripping square wave voltammetry
title_full Determination of cercosporin (CER) phytotoxin isolated from infected peanut leaves by using adsorptive stripping square wave voltammetry
title_fullStr Determination of cercosporin (CER) phytotoxin isolated from infected peanut leaves by using adsorptive stripping square wave voltammetry
title_full_unstemmed Determination of cercosporin (CER) phytotoxin isolated from infected peanut leaves by using adsorptive stripping square wave voltammetry
title_sort Determination of cercosporin (CER) phytotoxin isolated from infected peanut leaves by using adsorptive stripping square wave voltammetry
dc.creator.none.fl_str_mv Marchiando, Nancy Cristina
Zon, María Alicia
Fernandez, Hector
author Marchiando, Nancy Cristina
author_facet Marchiando, Nancy Cristina
Zon, María Alicia
Fernandez, Hector
author_role author
author2 Zon, María Alicia
Fernandez, Hector
author2_role author
author
dc.subject.none.fl_str_mv Cercosporin
Phytotoxins
Peanut
Square wave voltammetry
Adsorptive accumulation
topic Cercosporin
Phytotoxins
Peanut
Square wave voltammetry
Adsorptive accumulation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The adsorptive accumulation of cercosporin (CER) phytotoxin on glassy carbon (GC) electrodes from 1 M HClO4 aqueous solutions is used to detect and quantify the phytotoxin isolated from infected leaf tissue collected from field-grown peanut in five locations in southern regions of the Provinces of Cordoba and San Luis, Argentina. The adsorptive stripping square wave voltammetry (ASSWV) is employed to ´ perform the quantitative determination of CER in naturally infected extracts. Thin layer chromatography (TLC) and HPLC were used to verify the presence of CER in extracts. Values of Rf = 0.23 and Rf = 0.15 were determined for CER by TLC when extracts were dissolved in ethyl acetate (EA) and acetone (Ac), respectively, while a retention time of 6.13 min (Ac) was determined by HPLC. The recovery percentage of CER using ASSWV was determined by employing healthy peanut leaf tissue spiked with standard CER. The application of standard addition method was used to determine the recovery percentage as well as the CER contamination level in infected extracts. A recovery percentage of 93.27% was obtained with a relative standard deviation of 21.8% for a solution concentration of 1.04 × 10−6 mol dm−3 (n = 11). The limit of detection for a signal to noise ratio of 3:1 was about 6 ppb and the quantitation limit was about 50 ppb. The pondered relative standard deviation of 9.5% was calculated for the ASSWV method. Results found for CER contamination level in extracts through the electroanalytical method here proposed are in reasonable agreement with those values determined by using HPLC measurements.
Fil: Marchiando, Nancy Cristina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Zon, María Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Fernandez, Hector. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
description The adsorptive accumulation of cercosporin (CER) phytotoxin on glassy carbon (GC) electrodes from 1 M HClO4 aqueous solutions is used to detect and quantify the phytotoxin isolated from infected leaf tissue collected from field-grown peanut in five locations in southern regions of the Provinces of Cordoba and San Luis, Argentina. The adsorptive stripping square wave voltammetry (ASSWV) is employed to ´ perform the quantitative determination of CER in naturally infected extracts. Thin layer chromatography (TLC) and HPLC were used to verify the presence of CER in extracts. Values of Rf = 0.23 and Rf = 0.15 were determined for CER by TLC when extracts were dissolved in ethyl acetate (EA) and acetone (Ac), respectively, while a retention time of 6.13 min (Ac) was determined by HPLC. The recovery percentage of CER using ASSWV was determined by employing healthy peanut leaf tissue spiked with standard CER. The application of standard addition method was used to determine the recovery percentage as well as the CER contamination level in infected extracts. A recovery percentage of 93.27% was obtained with a relative standard deviation of 21.8% for a solution concentration of 1.04 × 10−6 mol dm−3 (n = 11). The limit of detection for a signal to noise ratio of 3:1 was about 6 ppb and the quantitation limit was about 50 ppb. The pondered relative standard deviation of 9.5% was calculated for the ASSWV method. Results found for CER contamination level in extracts through the electroanalytical method here proposed are in reasonable agreement with those values determined by using HPLC measurements.
publishDate 2005
dc.date.none.fl_str_mv 2005-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/241839
Marchiando, Nancy Cristina; Zon, María Alicia; Fernandez, Hector; Determination of cercosporin (CER) phytotoxin isolated from infected peanut leaves by using adsorptive stripping square wave voltammetry; Elsevier Science; Analytica Chimica Acta; 550; 1-2; 9-2005; 199-203
0003-2670
CONICET Digital
CONICET
url http://hdl.handle.net/11336/241839
identifier_str_mv Marchiando, Nancy Cristina; Zon, María Alicia; Fernandez, Hector; Determination of cercosporin (CER) phytotoxin isolated from infected peanut leaves by using adsorptive stripping square wave voltammetry; Elsevier Science; Analytica Chimica Acta; 550; 1-2; 9-2005; 199-203
0003-2670
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S000326700501069X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aca.2005.06.054
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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