Mixotrophic haptophytes are key bacterial grazers in oligotrophic coastal waters

Autores
Unrein, Fernando; Gasol, Josep M.; Not, Fabrice; Forn, Irene; Massana, Ramon
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Grazing rate estimates indicate that approximately half of the bacterivory in oligotrophic oceans is due to mixotrophic flagellates. However, most estimations have considered algae as a single group. Here we aimed at opening the black-box of the phytoflagellates (PF) <20 µm. Haptophytes, chlorophytes, cryptophytes and pigmented dinoflagellates were identified using fluorescent in situ hybridization or by standard DAPI-staining. Their fluctuations in abundance, cell-size, biomass, and bacterivory rates were measured through an annual cycle in an oligotrophic coastal system. On average, we were able to assign to these groups 37% of the total pico-phytoflagellates and 65% of the nano-phytoflagellates composition. Chlorophytes were mostly picoplanktonic and they never ingested fluorescently labelled bacteria. About 50% of the PF <20 µm biomass was represented by mixotrophic algae. Pigmented dinoflagellates were the least abundant group with little impact on bacterioplankton. Cryptophytes were quantitatively important during the coldest periods and explained about 4% of total bacterivory. Haptophytes were the most important mixotrophic group: (i) they were mostly represented by cells 3-5 µm in size present year-round; (ii) cell-specific grazing rates were comparable to other bacterivorous non-photosynthetic organisms, regardless of the in situ nutrient availability conditions; (iii) this group could acquire a significant portion of their carbon by ingesting bacteria; and (iv) haptophytes explained on average 40% of the bacterivory exerted by mixotrophic flagellates and were responsible for 9-27% of total bacterivory. Our results, when considered alongside the widespread distribution of haptophytes in the ocean, indicate that they play a key role as bacterivores in marine ecosystems.
Fil: Unrein, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; España
Fil: Gasol, Josep M.. Universite Pierre et Marie Curie; Francia. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; España
Fil: Not, Fabrice. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; España
Fil: Forn, Irene. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; España
Fil: Massana, Ramon. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; España
Materia
Mixotrophic
Haptophytes
Cryptophytes
Oligotrophic Coastal Seawater
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24628

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spelling Mixotrophic haptophytes are key bacterial grazers in oligotrophic coastal watersUnrein, FernandoGasol, Josep M.Not, FabriceForn, IreneMassana, RamonMixotrophicHaptophytesCryptophytesOligotrophic Coastal Seawaterhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Grazing rate estimates indicate that approximately half of the bacterivory in oligotrophic oceans is due to mixotrophic flagellates. However, most estimations have considered algae as a single group. Here we aimed at opening the black-box of the phytoflagellates (PF) <20 µm. Haptophytes, chlorophytes, cryptophytes and pigmented dinoflagellates were identified using fluorescent in situ hybridization or by standard DAPI-staining. Their fluctuations in abundance, cell-size, biomass, and bacterivory rates were measured through an annual cycle in an oligotrophic coastal system. On average, we were able to assign to these groups 37% of the total pico-phytoflagellates and 65% of the nano-phytoflagellates composition. Chlorophytes were mostly picoplanktonic and they never ingested fluorescently labelled bacteria. About 50% of the PF <20 µm biomass was represented by mixotrophic algae. Pigmented dinoflagellates were the least abundant group with little impact on bacterioplankton. Cryptophytes were quantitatively important during the coldest periods and explained about 4% of total bacterivory. Haptophytes were the most important mixotrophic group: (i) they were mostly represented by cells 3-5 µm in size present year-round; (ii) cell-specific grazing rates were comparable to other bacterivorous non-photosynthetic organisms, regardless of the in situ nutrient availability conditions; (iii) this group could acquire a significant portion of their carbon by ingesting bacteria; and (iv) haptophytes explained on average 40% of the bacterivory exerted by mixotrophic flagellates and were responsible for 9-27% of total bacterivory. Our results, when considered alongside the widespread distribution of haptophytes in the ocean, indicate that they play a key role as bacterivores in marine ecosystems.Fil: Unrein, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; EspañaFil: Gasol, Josep M.. Universite Pierre et Marie Curie; Francia. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; EspañaFil: Not, Fabrice. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; EspañaFil: Forn, Irene. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; EspañaFil: Massana, Ramon. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; EspañaNature Publishing Group2013-08-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24628Unrein, Fernando; Gasol, Josep M.; Not, Fabrice; Forn, Irene; Massana, Ramon; Mixotrophic haptophytes are key bacterial grazers in oligotrophic coastal waters; Nature Publishing Group; Isme Journal; 8; 8-8-2013; 164-1761751-7362CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/ismej.2013.132info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/ismej/journal/v8/n1/full/ismej2013132a.htmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:53:15Zoai:ri.conicet.gov.ar:11336/24628instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:53:16.042CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mixotrophic haptophytes are key bacterial grazers in oligotrophic coastal waters
title Mixotrophic haptophytes are key bacterial grazers in oligotrophic coastal waters
spellingShingle Mixotrophic haptophytes are key bacterial grazers in oligotrophic coastal waters
Unrein, Fernando
Mixotrophic
Haptophytes
Cryptophytes
Oligotrophic Coastal Seawater
title_short Mixotrophic haptophytes are key bacterial grazers in oligotrophic coastal waters
title_full Mixotrophic haptophytes are key bacterial grazers in oligotrophic coastal waters
title_fullStr Mixotrophic haptophytes are key bacterial grazers in oligotrophic coastal waters
title_full_unstemmed Mixotrophic haptophytes are key bacterial grazers in oligotrophic coastal waters
title_sort Mixotrophic haptophytes are key bacterial grazers in oligotrophic coastal waters
dc.creator.none.fl_str_mv Unrein, Fernando
Gasol, Josep M.
Not, Fabrice
Forn, Irene
Massana, Ramon
author Unrein, Fernando
author_facet Unrein, Fernando
Gasol, Josep M.
Not, Fabrice
Forn, Irene
Massana, Ramon
author_role author
author2 Gasol, Josep M.
Not, Fabrice
Forn, Irene
Massana, Ramon
author2_role author
author
author
author
dc.subject.none.fl_str_mv Mixotrophic
Haptophytes
Cryptophytes
Oligotrophic Coastal Seawater
topic Mixotrophic
Haptophytes
Cryptophytes
Oligotrophic Coastal Seawater
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Grazing rate estimates indicate that approximately half of the bacterivory in oligotrophic oceans is due to mixotrophic flagellates. However, most estimations have considered algae as a single group. Here we aimed at opening the black-box of the phytoflagellates (PF) <20 µm. Haptophytes, chlorophytes, cryptophytes and pigmented dinoflagellates were identified using fluorescent in situ hybridization or by standard DAPI-staining. Their fluctuations in abundance, cell-size, biomass, and bacterivory rates were measured through an annual cycle in an oligotrophic coastal system. On average, we were able to assign to these groups 37% of the total pico-phytoflagellates and 65% of the nano-phytoflagellates composition. Chlorophytes were mostly picoplanktonic and they never ingested fluorescently labelled bacteria. About 50% of the PF <20 µm biomass was represented by mixotrophic algae. Pigmented dinoflagellates were the least abundant group with little impact on bacterioplankton. Cryptophytes were quantitatively important during the coldest periods and explained about 4% of total bacterivory. Haptophytes were the most important mixotrophic group: (i) they were mostly represented by cells 3-5 µm in size present year-round; (ii) cell-specific grazing rates were comparable to other bacterivorous non-photosynthetic organisms, regardless of the in situ nutrient availability conditions; (iii) this group could acquire a significant portion of their carbon by ingesting bacteria; and (iv) haptophytes explained on average 40% of the bacterivory exerted by mixotrophic flagellates and were responsible for 9-27% of total bacterivory. Our results, when considered alongside the widespread distribution of haptophytes in the ocean, indicate that they play a key role as bacterivores in marine ecosystems.
Fil: Unrein, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; España
Fil: Gasol, Josep M.. Universite Pierre et Marie Curie; Francia. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; España
Fil: Not, Fabrice. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; España
Fil: Forn, Irene. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; España
Fil: Massana, Ramon. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; España
description Grazing rate estimates indicate that approximately half of the bacterivory in oligotrophic oceans is due to mixotrophic flagellates. However, most estimations have considered algae as a single group. Here we aimed at opening the black-box of the phytoflagellates (PF) <20 µm. Haptophytes, chlorophytes, cryptophytes and pigmented dinoflagellates were identified using fluorescent in situ hybridization or by standard DAPI-staining. Their fluctuations in abundance, cell-size, biomass, and bacterivory rates were measured through an annual cycle in an oligotrophic coastal system. On average, we were able to assign to these groups 37% of the total pico-phytoflagellates and 65% of the nano-phytoflagellates composition. Chlorophytes were mostly picoplanktonic and they never ingested fluorescently labelled bacteria. About 50% of the PF <20 µm biomass was represented by mixotrophic algae. Pigmented dinoflagellates were the least abundant group with little impact on bacterioplankton. Cryptophytes were quantitatively important during the coldest periods and explained about 4% of total bacterivory. Haptophytes were the most important mixotrophic group: (i) they were mostly represented by cells 3-5 µm in size present year-round; (ii) cell-specific grazing rates were comparable to other bacterivorous non-photosynthetic organisms, regardless of the in situ nutrient availability conditions; (iii) this group could acquire a significant portion of their carbon by ingesting bacteria; and (iv) haptophytes explained on average 40% of the bacterivory exerted by mixotrophic flagellates and were responsible for 9-27% of total bacterivory. Our results, when considered alongside the widespread distribution of haptophytes in the ocean, indicate that they play a key role as bacterivores in marine ecosystems.
publishDate 2013
dc.date.none.fl_str_mv 2013-08-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24628
Unrein, Fernando; Gasol, Josep M.; Not, Fabrice; Forn, Irene; Massana, Ramon; Mixotrophic haptophytes are key bacterial grazers in oligotrophic coastal waters; Nature Publishing Group; Isme Journal; 8; 8-8-2013; 164-176
1751-7362
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24628
identifier_str_mv Unrein, Fernando; Gasol, Josep M.; Not, Fabrice; Forn, Irene; Massana, Ramon; Mixotrophic haptophytes are key bacterial grazers in oligotrophic coastal waters; Nature Publishing Group; Isme Journal; 8; 8-8-2013; 164-176
1751-7362
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/ismej.2013.132
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/ismej/journal/v8/n1/full/ismej2013132a.html
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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