In situ prey selection of mixotrophic and heterotrophic flagellates in Antarctic oligotrophic lakes: An analysis of the digestive vacuole content
- Autores
- Gerea, M.; Queimaliños, C.; Schiaffino, M.R.; Izaguirre, I.; Forn, I.; Massana, R.; Unrein, F.
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- We investigated the selective predation of mixotrophic and heterotrophic flagellates (MF and HF) on different heterotrophic prokaryote phylotypes (HPP; Bacteria + Archaea) living in natural assemblages from oligotrophic Antarctic lakes. In situ prey preference was analyzed for the first time on different mixotrophic taxa (Pseudopedinella sp., Ochromonas-like cells, Chrysophyceae >5 μm). The relative abundances of seven different HPP hybridized by CARD-FISH (catalyzed reporter deposition-fluorescent in situ hybridization) in natural community were compared with the proportions of hybridized cells inside digestive vacuoles. Our results showed some general trends to selectivity over some HPP. Alphaproteobacteria and Betaproteobacteria were the most abundant groups, and strikingly, a negative selection trend was detected in most samples by all bacterivorous protists. In contrast, for Actinobacteria a positive selection trend was observed in most samples, whereas Bacteroidetes seemed to be randomly preyed upon. Interestingly, similar prey preferences were observed in all bacterivorous flagellates. Our results suggest that phylogenetic affiliation determines part of the process of prey selection by protists in these lakes. Nevertheless, other features, such as cell size, morphology and the presence of the S-layer, might also significantly contribute to prey selectivity on the HPP. © 2012 The Author 2012. Published by Oxford University Press. All rights reserved.
Fil:Queimaliños, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Schiaffino, M.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Izaguirre, I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Unrein, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. - Fuente
- J. Plankton Res. 2013;35(1):201-212
- Materia
-
CARD-FISH
Heterotrophic flagellates
Mixotrophic flagellates
Oligotrophic lakes
Prey selection
catalysis
digestive system
flagellate
heterotrophy
lake ecosystem
mixotrophy
morphology
oligotrophic environment
phylogenetics
phytoplankton
predation
prey selection
prokaryote
protist
Antarctica
Actinobacteria
Alphaproteobacteria
Archaea
Bacteria (microorganisms)
Bacteroidetes
Betaproteobacteria
Chrysophyceae
Mastigophora (flagellates)
Ochromonas
Prokaryota
Protista
Pseudopedinella - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/2.5/ar
- Repositorio
- Institución
- Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
- OAI Identificador
- paperaa:paper_01427873_v35_n1_p201_Gerea
Ver los metadatos del registro completo
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repository_id_str |
1896 |
network_name_str |
Biblioteca Digital (UBA-FCEN) |
spelling |
In situ prey selection of mixotrophic and heterotrophic flagellates in Antarctic oligotrophic lakes: An analysis of the digestive vacuole contentGerea, M.Queimaliños, C.Schiaffino, M.R.Izaguirre, I.Forn, I.Massana, R.Unrein, F.CARD-FISHHeterotrophic flagellatesMixotrophic flagellatesOligotrophic lakesPrey selectioncatalysisdigestive systemflagellateheterotrophylake ecosystemmixotrophymorphologyoligotrophic environmentphylogeneticsphytoplanktonpredationprey selectionprokaryoteprotistAntarcticaActinobacteriaAlphaproteobacteriaArchaeaBacteria (microorganisms)BacteroidetesBetaproteobacteriaChrysophyceaeMastigophora (flagellates)OchromonasProkaryotaProtistaPseudopedinellaWe investigated the selective predation of mixotrophic and heterotrophic flagellates (MF and HF) on different heterotrophic prokaryote phylotypes (HPP; Bacteria + Archaea) living in natural assemblages from oligotrophic Antarctic lakes. In situ prey preference was analyzed for the first time on different mixotrophic taxa (Pseudopedinella sp., Ochromonas-like cells, Chrysophyceae >5 μm). The relative abundances of seven different HPP hybridized by CARD-FISH (catalyzed reporter deposition-fluorescent in situ hybridization) in natural community were compared with the proportions of hybridized cells inside digestive vacuoles. Our results showed some general trends to selectivity over some HPP. Alphaproteobacteria and Betaproteobacteria were the most abundant groups, and strikingly, a negative selection trend was detected in most samples by all bacterivorous protists. In contrast, for Actinobacteria a positive selection trend was observed in most samples, whereas Bacteroidetes seemed to be randomly preyed upon. Interestingly, similar prey preferences were observed in all bacterivorous flagellates. Our results suggest that phylogenetic affiliation determines part of the process of prey selection by protists in these lakes. Nevertheless, other features, such as cell size, morphology and the presence of the S-layer, might also significantly contribute to prey selectivity on the HPP. © 2012 The Author 2012. Published by Oxford University Press. All rights reserved.Fil:Queimaliños, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Schiaffino, M.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Izaguirre, I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Unrein, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_01427873_v35_n1_p201_GereaJ. Plankton Res. 2013;35(1):201-212reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-29T13:42:52Zpaperaa:paper_01427873_v35_n1_p201_GereaInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-29 13:42:53.618Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse |
dc.title.none.fl_str_mv |
In situ prey selection of mixotrophic and heterotrophic flagellates in Antarctic oligotrophic lakes: An analysis of the digestive vacuole content |
title |
In situ prey selection of mixotrophic and heterotrophic flagellates in Antarctic oligotrophic lakes: An analysis of the digestive vacuole content |
spellingShingle |
In situ prey selection of mixotrophic and heterotrophic flagellates in Antarctic oligotrophic lakes: An analysis of the digestive vacuole content Gerea, M. CARD-FISH Heterotrophic flagellates Mixotrophic flagellates Oligotrophic lakes Prey selection catalysis digestive system flagellate heterotrophy lake ecosystem mixotrophy morphology oligotrophic environment phylogenetics phytoplankton predation prey selection prokaryote protist Antarctica Actinobacteria Alphaproteobacteria Archaea Bacteria (microorganisms) Bacteroidetes Betaproteobacteria Chrysophyceae Mastigophora (flagellates) Ochromonas Prokaryota Protista Pseudopedinella |
title_short |
In situ prey selection of mixotrophic and heterotrophic flagellates in Antarctic oligotrophic lakes: An analysis of the digestive vacuole content |
title_full |
In situ prey selection of mixotrophic and heterotrophic flagellates in Antarctic oligotrophic lakes: An analysis of the digestive vacuole content |
title_fullStr |
In situ prey selection of mixotrophic and heterotrophic flagellates in Antarctic oligotrophic lakes: An analysis of the digestive vacuole content |
title_full_unstemmed |
In situ prey selection of mixotrophic and heterotrophic flagellates in Antarctic oligotrophic lakes: An analysis of the digestive vacuole content |
title_sort |
In situ prey selection of mixotrophic and heterotrophic flagellates in Antarctic oligotrophic lakes: An analysis of the digestive vacuole content |
dc.creator.none.fl_str_mv |
Gerea, M. Queimaliños, C. Schiaffino, M.R. Izaguirre, I. Forn, I. Massana, R. Unrein, F. |
author |
Gerea, M. |
author_facet |
Gerea, M. Queimaliños, C. Schiaffino, M.R. Izaguirre, I. Forn, I. Massana, R. Unrein, F. |
author_role |
author |
author2 |
Queimaliños, C. Schiaffino, M.R. Izaguirre, I. Forn, I. Massana, R. Unrein, F. |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
CARD-FISH Heterotrophic flagellates Mixotrophic flagellates Oligotrophic lakes Prey selection catalysis digestive system flagellate heterotrophy lake ecosystem mixotrophy morphology oligotrophic environment phylogenetics phytoplankton predation prey selection prokaryote protist Antarctica Actinobacteria Alphaproteobacteria Archaea Bacteria (microorganisms) Bacteroidetes Betaproteobacteria Chrysophyceae Mastigophora (flagellates) Ochromonas Prokaryota Protista Pseudopedinella |
topic |
CARD-FISH Heterotrophic flagellates Mixotrophic flagellates Oligotrophic lakes Prey selection catalysis digestive system flagellate heterotrophy lake ecosystem mixotrophy morphology oligotrophic environment phylogenetics phytoplankton predation prey selection prokaryote protist Antarctica Actinobacteria Alphaproteobacteria Archaea Bacteria (microorganisms) Bacteroidetes Betaproteobacteria Chrysophyceae Mastigophora (flagellates) Ochromonas Prokaryota Protista Pseudopedinella |
dc.description.none.fl_txt_mv |
We investigated the selective predation of mixotrophic and heterotrophic flagellates (MF and HF) on different heterotrophic prokaryote phylotypes (HPP; Bacteria + Archaea) living in natural assemblages from oligotrophic Antarctic lakes. In situ prey preference was analyzed for the first time on different mixotrophic taxa (Pseudopedinella sp., Ochromonas-like cells, Chrysophyceae >5 μm). The relative abundances of seven different HPP hybridized by CARD-FISH (catalyzed reporter deposition-fluorescent in situ hybridization) in natural community were compared with the proportions of hybridized cells inside digestive vacuoles. Our results showed some general trends to selectivity over some HPP. Alphaproteobacteria and Betaproteobacteria were the most abundant groups, and strikingly, a negative selection trend was detected in most samples by all bacterivorous protists. In contrast, for Actinobacteria a positive selection trend was observed in most samples, whereas Bacteroidetes seemed to be randomly preyed upon. Interestingly, similar prey preferences were observed in all bacterivorous flagellates. Our results suggest that phylogenetic affiliation determines part of the process of prey selection by protists in these lakes. Nevertheless, other features, such as cell size, morphology and the presence of the S-layer, might also significantly contribute to prey selectivity on the HPP. © 2012 The Author 2012. Published by Oxford University Press. All rights reserved. Fil:Queimaliños, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Schiaffino, M.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Izaguirre, I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Unrein, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. |
description |
We investigated the selective predation of mixotrophic and heterotrophic flagellates (MF and HF) on different heterotrophic prokaryote phylotypes (HPP; Bacteria + Archaea) living in natural assemblages from oligotrophic Antarctic lakes. In situ prey preference was analyzed for the first time on different mixotrophic taxa (Pseudopedinella sp., Ochromonas-like cells, Chrysophyceae >5 μm). The relative abundances of seven different HPP hybridized by CARD-FISH (catalyzed reporter deposition-fluorescent in situ hybridization) in natural community were compared with the proportions of hybridized cells inside digestive vacuoles. Our results showed some general trends to selectivity over some HPP. Alphaproteobacteria and Betaproteobacteria were the most abundant groups, and strikingly, a negative selection trend was detected in most samples by all bacterivorous protists. In contrast, for Actinobacteria a positive selection trend was observed in most samples, whereas Bacteroidetes seemed to be randomly preyed upon. Interestingly, similar prey preferences were observed in all bacterivorous flagellates. Our results suggest that phylogenetic affiliation determines part of the process of prey selection by protists in these lakes. Nevertheless, other features, such as cell size, morphology and the presence of the S-layer, might also significantly contribute to prey selectivity on the HPP. © 2012 The Author 2012. Published by Oxford University Press. All rights reserved. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12110/paper_01427873_v35_n1_p201_Gerea |
url |
http://hdl.handle.net/20.500.12110/paper_01427873_v35_n1_p201_Gerea |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/2.5/ar |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
J. Plankton Res. 2013;35(1):201-212 reponame:Biblioteca Digital (UBA-FCEN) instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales instacron:UBA-FCEN |
reponame_str |
Biblioteca Digital (UBA-FCEN) |
collection |
Biblioteca Digital (UBA-FCEN) |
instname_str |
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
instacron_str |
UBA-FCEN |
institution |
UBA-FCEN |
repository.name.fl_str_mv |
Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
repository.mail.fl_str_mv |
ana@bl.fcen.uba.ar |
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13.070432 |