Comparative proteomics of cerebrospinal fluid in neuropathologically- confirmed Alzheimer's disease and non-demented elderly subjects

Autores
Castaño, Eduardo Miguel; Roher, Alex E.; Esh, Chera L.; Kokjohn, Tyler A.; Beach, Thomas
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objectives: Diagnostic tests able to reveal Alzheimer's disease (AD) in living patients before cognitive ability is destroyed are urgently needed. Such tests must distinguish AD from other dementia causes, as well as differentiate subtle changes associated with normal aging from true pathology emergence. A single biomarker offering such diagnostic and prognostic capacities has eluded identification. Therefore, a valuable test for AD is likely to be based on a specific pattern of change in a set of proteins, rather than a single protein. Methods: We examined pooled cerebrospinal fluid (CSF) samples obtained from neuropathologically-confirmed AD (n=43) and non-demented control subjects (n=43) using 2-dimensional gel electrophoresis (2DE) proteomic methodology to detect differentially expressed proteins. Proteins exhibiting expression level differences between the pools were recovered and identified using matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry. Results: Five differentially-expressed proteins with potential roles in amyloid-β metabolism and vascular and brain physiology [apolipoprotein A-1 (Apo A-1), cathepsin D (CatD), hemopexin (HPX), transthyretin (TTR), and two pigment epithelium-derived factor (PEDF) isoforms] were identified. Apo A-1, CatD and TTR were significantly reduced in the AD pool sample, while HPX and the PEDF isoforms were increased in AD CSF. Discussion: These results suggest that multi-factor proteomic pattern analysis of the CSF may provide a means to diagnose and assess AD. © 2006 W. S. Maney & Son Ltd.
Fil: Castaño, Eduardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Sun Health Research Institute; Estados Unidos
Fil: Roher, Alex E.. Sun Health Research Institute; Estados Unidos
Fil: Esh, Chera L.. Sun Health Research Institute; Estados Unidos
Fil: Kokjohn, Tyler A.. Sun Health Research Institute; Estados Unidos
Fil: Beach, Thomas. Sun Health Research Institute; Estados Unidos
Materia
Alzheimer'S Disease
Biomarkers
Cerebrospinal Fluid
Proteomics
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/39061
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Comparative proteomics of cerebrospinal fluid in neuropathologically- confirmed Alzheimer's disease and non-demented elderly subjectsCastaño, Eduardo MiguelRoher, Alex E.Esh, Chera L.Kokjohn, Tyler A.Beach, ThomasAlzheimer'S DiseaseBiomarkersCerebrospinal FluidProteomicshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Objectives: Diagnostic tests able to reveal Alzheimer's disease (AD) in living patients before cognitive ability is destroyed are urgently needed. Such tests must distinguish AD from other dementia causes, as well as differentiate subtle changes associated with normal aging from true pathology emergence. A single biomarker offering such diagnostic and prognostic capacities has eluded identification. Therefore, a valuable test for AD is likely to be based on a specific pattern of change in a set of proteins, rather than a single protein. Methods: We examined pooled cerebrospinal fluid (CSF) samples obtained from neuropathologically-confirmed AD (n=43) and non-demented control subjects (n=43) using 2-dimensional gel electrophoresis (2DE) proteomic methodology to detect differentially expressed proteins. Proteins exhibiting expression level differences between the pools were recovered and identified using matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry. Results: Five differentially-expressed proteins with potential roles in amyloid-β metabolism and vascular and brain physiology [apolipoprotein A-1 (Apo A-1), cathepsin D (CatD), hemopexin (HPX), transthyretin (TTR), and two pigment epithelium-derived factor (PEDF) isoforms] were identified. Apo A-1, CatD and TTR were significantly reduced in the AD pool sample, while HPX and the PEDF isoforms were increased in AD CSF. Discussion: These results suggest that multi-factor proteomic pattern analysis of the CSF may provide a means to diagnose and assess AD. © 2006 W. S. Maney & Son Ltd.Fil: Castaño, Eduardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Sun Health Research Institute; Estados UnidosFil: Roher, Alex E.. Sun Health Research Institute; Estados UnidosFil: Esh, Chera L.. Sun Health Research Institute; Estados UnidosFil: Kokjohn, Tyler A.. Sun Health Research Institute; Estados UnidosFil: Beach, Thomas. Sun Health Research Institute; Estados UnidosManey Publishing2006-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/39061Castaño, Eduardo Miguel; Roher, Alex E.; Esh, Chera L.; Kokjohn, Tyler A.; Beach, Thomas; Comparative proteomics of cerebrospinal fluid in neuropathologically- confirmed Alzheimer's disease and non-demented elderly subjects; Maney Publishing; Neurological Research; 28; 2; 3-2006; 155-1630161-64121743-1328CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1179/016164106X98035info:eu-repo/semantics/altIdentifier/doi/10.1179/016164106X98035info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:03:33Zoai:ri.conicet.gov.ar:11336/39061instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:03:33.391CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Comparative proteomics of cerebrospinal fluid in neuropathologically- confirmed Alzheimer's disease and non-demented elderly subjects
title Comparative proteomics of cerebrospinal fluid in neuropathologically- confirmed Alzheimer's disease and non-demented elderly subjects
spellingShingle Comparative proteomics of cerebrospinal fluid in neuropathologically- confirmed Alzheimer's disease and non-demented elderly subjects
Castaño, Eduardo Miguel
Alzheimer'S Disease
Biomarkers
Cerebrospinal Fluid
Proteomics
title_short Comparative proteomics of cerebrospinal fluid in neuropathologically- confirmed Alzheimer's disease and non-demented elderly subjects
title_full Comparative proteomics of cerebrospinal fluid in neuropathologically- confirmed Alzheimer's disease and non-demented elderly subjects
title_fullStr Comparative proteomics of cerebrospinal fluid in neuropathologically- confirmed Alzheimer's disease and non-demented elderly subjects
title_full_unstemmed Comparative proteomics of cerebrospinal fluid in neuropathologically- confirmed Alzheimer's disease and non-demented elderly subjects
title_sort Comparative proteomics of cerebrospinal fluid in neuropathologically- confirmed Alzheimer's disease and non-demented elderly subjects
dc.creator.none.fl_str_mv Castaño, Eduardo Miguel
Roher, Alex E.
Esh, Chera L.
Kokjohn, Tyler A.
Beach, Thomas
author Castaño, Eduardo Miguel
author_facet Castaño, Eduardo Miguel
Roher, Alex E.
Esh, Chera L.
Kokjohn, Tyler A.
Beach, Thomas
author_role author
author2 Roher, Alex E.
Esh, Chera L.
Kokjohn, Tyler A.
Beach, Thomas
author2_role author
author
author
author
dc.subject.none.fl_str_mv Alzheimer'S Disease
Biomarkers
Cerebrospinal Fluid
Proteomics
topic Alzheimer'S Disease
Biomarkers
Cerebrospinal Fluid
Proteomics
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Objectives: Diagnostic tests able to reveal Alzheimer's disease (AD) in living patients before cognitive ability is destroyed are urgently needed. Such tests must distinguish AD from other dementia causes, as well as differentiate subtle changes associated with normal aging from true pathology emergence. A single biomarker offering such diagnostic and prognostic capacities has eluded identification. Therefore, a valuable test for AD is likely to be based on a specific pattern of change in a set of proteins, rather than a single protein. Methods: We examined pooled cerebrospinal fluid (CSF) samples obtained from neuropathologically-confirmed AD (n=43) and non-demented control subjects (n=43) using 2-dimensional gel electrophoresis (2DE) proteomic methodology to detect differentially expressed proteins. Proteins exhibiting expression level differences between the pools were recovered and identified using matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry. Results: Five differentially-expressed proteins with potential roles in amyloid-β metabolism and vascular and brain physiology [apolipoprotein A-1 (Apo A-1), cathepsin D (CatD), hemopexin (HPX), transthyretin (TTR), and two pigment epithelium-derived factor (PEDF) isoforms] were identified. Apo A-1, CatD and TTR were significantly reduced in the AD pool sample, while HPX and the PEDF isoforms were increased in AD CSF. Discussion: These results suggest that multi-factor proteomic pattern analysis of the CSF may provide a means to diagnose and assess AD. © 2006 W. S. Maney & Son Ltd.
Fil: Castaño, Eduardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Sun Health Research Institute; Estados Unidos
Fil: Roher, Alex E.. Sun Health Research Institute; Estados Unidos
Fil: Esh, Chera L.. Sun Health Research Institute; Estados Unidos
Fil: Kokjohn, Tyler A.. Sun Health Research Institute; Estados Unidos
Fil: Beach, Thomas. Sun Health Research Institute; Estados Unidos
description Objectives: Diagnostic tests able to reveal Alzheimer's disease (AD) in living patients before cognitive ability is destroyed are urgently needed. Such tests must distinguish AD from other dementia causes, as well as differentiate subtle changes associated with normal aging from true pathology emergence. A single biomarker offering such diagnostic and prognostic capacities has eluded identification. Therefore, a valuable test for AD is likely to be based on a specific pattern of change in a set of proteins, rather than a single protein. Methods: We examined pooled cerebrospinal fluid (CSF) samples obtained from neuropathologically-confirmed AD (n=43) and non-demented control subjects (n=43) using 2-dimensional gel electrophoresis (2DE) proteomic methodology to detect differentially expressed proteins. Proteins exhibiting expression level differences between the pools were recovered and identified using matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry. Results: Five differentially-expressed proteins with potential roles in amyloid-β metabolism and vascular and brain physiology [apolipoprotein A-1 (Apo A-1), cathepsin D (CatD), hemopexin (HPX), transthyretin (TTR), and two pigment epithelium-derived factor (PEDF) isoforms] were identified. Apo A-1, CatD and TTR were significantly reduced in the AD pool sample, while HPX and the PEDF isoforms were increased in AD CSF. Discussion: These results suggest that multi-factor proteomic pattern analysis of the CSF may provide a means to diagnose and assess AD. © 2006 W. S. Maney & Son Ltd.
publishDate 2006
dc.date.none.fl_str_mv 2006-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/39061
Castaño, Eduardo Miguel; Roher, Alex E.; Esh, Chera L.; Kokjohn, Tyler A.; Beach, Thomas; Comparative proteomics of cerebrospinal fluid in neuropathologically- confirmed Alzheimer's disease and non-demented elderly subjects; Maney Publishing; Neurological Research; 28; 2; 3-2006; 155-163
0161-6412
1743-1328
CONICET Digital
CONICET
url http://hdl.handle.net/11336/39061
identifier_str_mv Castaño, Eduardo Miguel; Roher, Alex E.; Esh, Chera L.; Kokjohn, Tyler A.; Beach, Thomas; Comparative proteomics of cerebrospinal fluid in neuropathologically- confirmed Alzheimer's disease and non-demented elderly subjects; Maney Publishing; Neurological Research; 28; 2; 3-2006; 155-163
0161-6412
1743-1328
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1179/016164106X98035
info:eu-repo/semantics/altIdentifier/doi/10.1179/016164106X98035
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Maney Publishing
publisher.none.fl_str_mv Maney Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.885934

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