Neurons born in the adult dentate gyrus form functional synapses with target cells

Autores
Toni, Nicolas; Laplagne, Diego Andres; Zhao, Chunmei; Lombardi, María Gabriela; Ribak, Charles E.; Gage, Fred H.; Schinder, Alejandro Fabián
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Adult neurogenesis occurs in the hippocampus and the olfactory bulb of the mammalian CNS. Recent studies have demonstrated that newborn granule cells of the adult hippocampus are postsynaptic targets of excitatory and inhibitory neurons, but evidence of synapse formation by the axons of these cells is still lacking. By combining retroviral expression of green fluorescent protein in adult-born neurons of the mouse dentate gyrus with immuno-electron microscopy, we found output synapses that were formed by labeled terminals on appropriate target cells in the CA3 area and the hilus. Furthermore, retroviral expression of channelrhodopsin-2 allowed us to light-stimulate newborn granule cells and identify postsynaptic target neurons by whole-cell recordings in acute slices. Our structural and functional evidence indicates that axons of adult-born granule cells establish synapses with hilar interneurons, mossy cells and CA3 pyramidal cells and release glutamate as their main neurotransmitter.
Fil: Toni, Nicolas. Salk Institute for Biological Studies; Estados Unidos
Fil: Laplagne, Diego Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Zhao, Chunmei. Salk Institute for Biological Studies; Estados Unidos
Fil: Lombardi, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Ribak, Charles E.. University of California at Irvine; Estados Unidos
Fil: Gage, Fred H.. Salk Institute for Biological Studies; Estados Unidos
Fil: Schinder, Alejandro Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Materia
Action Potentials/Drug Effects
Action Potentials/Physiology
Action Potentials/Radiation Effects
Animals
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/42708
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/42708
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Neurons born in the adult dentate gyrus form functional synapses with target cellsToni, NicolasLaplagne, Diego AndresZhao, ChunmeiLombardi, María GabrielaRibak, Charles E.Gage, Fred H.Schinder, Alejandro FabiánAction Potentials/Drug EffectsAction Potentials/PhysiologyAction Potentials/Radiation EffectsAnimalsAdult neurogenesis occurs in the hippocampus and the olfactory bulb of the mammalian CNS. Recent studies have demonstrated that newborn granule cells of the adult hippocampus are postsynaptic targets of excitatory and inhibitory neurons, but evidence of synapse formation by the axons of these cells is still lacking. By combining retroviral expression of green fluorescent protein in adult-born neurons of the mouse dentate gyrus with immuno-electron microscopy, we found output synapses that were formed by labeled terminals on appropriate target cells in the CA3 area and the hilus. Furthermore, retroviral expression of channelrhodopsin-2 allowed us to light-stimulate newborn granule cells and identify postsynaptic target neurons by whole-cell recordings in acute slices. Our structural and functional evidence indicates that axons of adult-born granule cells establish synapses with hilar interneurons, mossy cells and CA3 pyramidal cells and release glutamate as their main neurotransmitter.Fil: Toni, Nicolas. Salk Institute for Biological Studies; Estados UnidosFil: Laplagne, Diego Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Zhao, Chunmei. Salk Institute for Biological Studies; Estados UnidosFil: Lombardi, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Ribak, Charles E.. University of California at Irvine; Estados UnidosFil: Gage, Fred H.. Salk Institute for Biological Studies; Estados UnidosFil: Schinder, Alejandro Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaNature Publishing Group2008info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/42708Toni, Nicolas; Laplagne, Diego Andres; Zhao, Chunmei; Lombardi, María Gabriela; Ribak, Charles E.; et al.; Neurons born in the adult dentate gyrus form functional synapses with target cells; Nature Publishing Group; Nature Neuroscience.; 11; 8; 2008; 901-9071097-62561546-1726CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/nn.2156info:eu-repo/semantics/altIdentifier/doi/10.1038/nn.2156info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2572641/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:36:56Zoai:ri.conicet.gov.ar:11336/42708instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:36:56.47CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Neurons born in the adult dentate gyrus form functional synapses with target cells
title Neurons born in the adult dentate gyrus form functional synapses with target cells
spellingShingle Neurons born in the adult dentate gyrus form functional synapses with target cells
Toni, Nicolas
Action Potentials/Drug Effects
Action Potentials/Physiology
Action Potentials/Radiation Effects
Animals
title_short Neurons born in the adult dentate gyrus form functional synapses with target cells
title_full Neurons born in the adult dentate gyrus form functional synapses with target cells
title_fullStr Neurons born in the adult dentate gyrus form functional synapses with target cells
title_full_unstemmed Neurons born in the adult dentate gyrus form functional synapses with target cells
title_sort Neurons born in the adult dentate gyrus form functional synapses with target cells
dc.creator.none.fl_str_mv Toni, Nicolas
Laplagne, Diego Andres
Zhao, Chunmei
Lombardi, María Gabriela
Ribak, Charles E.
Gage, Fred H.
Schinder, Alejandro Fabián
author Toni, Nicolas
author_facet Toni, Nicolas
Laplagne, Diego Andres
Zhao, Chunmei
Lombardi, María Gabriela
Ribak, Charles E.
Gage, Fred H.
Schinder, Alejandro Fabián
author_role author
author2 Laplagne, Diego Andres
Zhao, Chunmei
Lombardi, María Gabriela
Ribak, Charles E.
Gage, Fred H.
Schinder, Alejandro Fabián
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Action Potentials/Drug Effects
Action Potentials/Physiology
Action Potentials/Radiation Effects
Animals
topic Action Potentials/Drug Effects
Action Potentials/Physiology
Action Potentials/Radiation Effects
Animals
dc.description.none.fl_txt_mv Adult neurogenesis occurs in the hippocampus and the olfactory bulb of the mammalian CNS. Recent studies have demonstrated that newborn granule cells of the adult hippocampus are postsynaptic targets of excitatory and inhibitory neurons, but evidence of synapse formation by the axons of these cells is still lacking. By combining retroviral expression of green fluorescent protein in adult-born neurons of the mouse dentate gyrus with immuno-electron microscopy, we found output synapses that were formed by labeled terminals on appropriate target cells in the CA3 area and the hilus. Furthermore, retroviral expression of channelrhodopsin-2 allowed us to light-stimulate newborn granule cells and identify postsynaptic target neurons by whole-cell recordings in acute slices. Our structural and functional evidence indicates that axons of adult-born granule cells establish synapses with hilar interneurons, mossy cells and CA3 pyramidal cells and release glutamate as their main neurotransmitter.
Fil: Toni, Nicolas. Salk Institute for Biological Studies; Estados Unidos
Fil: Laplagne, Diego Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Zhao, Chunmei. Salk Institute for Biological Studies; Estados Unidos
Fil: Lombardi, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Ribak, Charles E.. University of California at Irvine; Estados Unidos
Fil: Gage, Fred H.. Salk Institute for Biological Studies; Estados Unidos
Fil: Schinder, Alejandro Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
description Adult neurogenesis occurs in the hippocampus and the olfactory bulb of the mammalian CNS. Recent studies have demonstrated that newborn granule cells of the adult hippocampus are postsynaptic targets of excitatory and inhibitory neurons, but evidence of synapse formation by the axons of these cells is still lacking. By combining retroviral expression of green fluorescent protein in adult-born neurons of the mouse dentate gyrus with immuno-electron microscopy, we found output synapses that were formed by labeled terminals on appropriate target cells in the CA3 area and the hilus. Furthermore, retroviral expression of channelrhodopsin-2 allowed us to light-stimulate newborn granule cells and identify postsynaptic target neurons by whole-cell recordings in acute slices. Our structural and functional evidence indicates that axons of adult-born granule cells establish synapses with hilar interneurons, mossy cells and CA3 pyramidal cells and release glutamate as their main neurotransmitter.
publishDate 2008
dc.date.none.fl_str_mv 2008
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/42708
Toni, Nicolas; Laplagne, Diego Andres; Zhao, Chunmei; Lombardi, María Gabriela; Ribak, Charles E.; et al.; Neurons born in the adult dentate gyrus form functional synapses with target cells; Nature Publishing Group; Nature Neuroscience.; 11; 8; 2008; 901-907
1097-6256
1546-1726
CONICET Digital
CONICET
url http://hdl.handle.net/11336/42708
identifier_str_mv Toni, Nicolas; Laplagne, Diego Andres; Zhao, Chunmei; Lombardi, María Gabriela; Ribak, Charles E.; et al.; Neurons born in the adult dentate gyrus form functional synapses with target cells; Nature Publishing Group; Nature Neuroscience.; 11; 8; 2008; 901-907
1097-6256
1546-1726
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/nn.2156
info:eu-repo/semantics/altIdentifier/doi/10.1038/nn.2156
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2572641/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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