Cl−/HCO3− Exchanger slc26a6: a ph regulator shapes the cardiac action potential

Autores
Aiello, Ernesto Alejandro; Casey, Joseph R.; Alvarez, Bernardo
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Regulation of intracellular pH (pHi) is often considered a ?housekeeping? function, contributing little to cardiac function. With the study published by Sirish et al7 in this issue of Circulation: Arrhythmia and Electrophysiology, a Cl-/HCO3- exchanger is revealed to have a more central role in cardiac function.Intracellular pH is an important modulator of cardiac excitation and contraction1, and adversely contributes to electrical arrhythmia2. Cardiac myocytes express a complex apparatus to regulate pHi. Cardiac muscle cytosolic pH (~7.2) is maintained by sarcolemmal ion transport proteins that move H+, OH− or HCO3− ions across the membrane3. Along with the acid extruders Na+/H+ exchanger (NHE1) and Na+/HCO3− cotransporter (NBC, electrogenic NBCe1/e2 and electroneutral NBCn1) myocytes possess Cl-/HCO3− exchangers (SLC4 family members AE1, AE2, and AE3) and Cl-/OH- exchanger (CHE, with no molecular identity) alkali extruders3. SLC26 gene family members were identified as (mouse slc26a64 and its human orthologue SLC26A65, and slc26a36) responsible for Cl-/HCO3− and Cl-/OH- at plasma membrane of heart ventricles6. The work of Sirish et al7 in this issue revealed that ablation of slc26a6, a plasma membrane Cl-/HCO3- exchange protein, results in cardiac action potential (AP) shortening, cardiomyocyte Ca2+ transient (CaT) and sarcoplasmic reticulum (SR) Ca2+ load reduction, cardiomyocyte diminution of sarcomeric shortening, and cardiomyocyte intracellular pH (pHi) elevation. Moreover, in slc26a6-/- mice these factors led to a reduction of cardiac fractional shortening and cardiac contractility responses, and alterations of cardiac conduction system, as seen in sinus bradycardia and fragmentation of the QRS electrocardiographic-recorded complex. Since slc26a6 has stoichiometry of 2 (or more) HCO3-: Cl-, its transport function is electrogenic, with significance to the cardiomyocyte membrane potential.
Fil: Aiello, Ernesto Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigaciones Cardiovasculares ; Argentina
Fil: Casey, Joseph R.. University Of Alberta. Faculty Of Medicine And Oral Health Sciences; Canadá
Fil: Alvarez, Bernardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigaciones Cardiovasculares ; Argentina
Materia
Action Potentials
Ion Transport
Physiology
Sarcoplasmatic Reticulum
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/49489

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oai_identifier_str oai:ri.conicet.gov.ar:11336/49489
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network_name_str CONICET Digital (CONICET)
spelling Cl−/HCO3− Exchanger slc26a6: a ph regulator shapes the cardiac action potentialAiello, Ernesto AlejandroCasey, Joseph R.Alvarez, BernardoAction PotentialsIon TransportPhysiologySarcoplasmatic Reticulumhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Regulation of intracellular pH (pHi) is often considered a ?housekeeping? function, contributing little to cardiac function. With the study published by Sirish et al7 in this issue of Circulation: Arrhythmia and Electrophysiology, a Cl-/HCO3- exchanger is revealed to have a more central role in cardiac function.Intracellular pH is an important modulator of cardiac excitation and contraction1, and adversely contributes to electrical arrhythmia2. Cardiac myocytes express a complex apparatus to regulate pHi. Cardiac muscle cytosolic pH (~7.2) is maintained by sarcolemmal ion transport proteins that move H+, OH− or HCO3− ions across the membrane3. Along with the acid extruders Na+/H+ exchanger (NHE1) and Na+/HCO3− cotransporter (NBC, electrogenic NBCe1/e2 and electroneutral NBCn1) myocytes possess Cl-/HCO3− exchangers (SLC4 family members AE1, AE2, and AE3) and Cl-/OH- exchanger (CHE, with no molecular identity) alkali extruders3. SLC26 gene family members were identified as (mouse slc26a64 and its human orthologue SLC26A65, and slc26a36) responsible for Cl-/HCO3− and Cl-/OH- at plasma membrane of heart ventricles6. The work of Sirish et al7 in this issue revealed that ablation of slc26a6, a plasma membrane Cl-/HCO3- exchange protein, results in cardiac action potential (AP) shortening, cardiomyocyte Ca2+ transient (CaT) and sarcoplasmic reticulum (SR) Ca2+ load reduction, cardiomyocyte diminution of sarcomeric shortening, and cardiomyocyte intracellular pH (pHi) elevation. Moreover, in slc26a6-/- mice these factors led to a reduction of cardiac fractional shortening and cardiac contractility responses, and alterations of cardiac conduction system, as seen in sinus bradycardia and fragmentation of the QRS electrocardiographic-recorded complex. Since slc26a6 has stoichiometry of 2 (or more) HCO3-: Cl-, its transport function is electrogenic, with significance to the cardiomyocyte membrane potential.Fil: Aiello, Ernesto Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigaciones Cardiovasculares ; ArgentinaFil: Casey, Joseph R.. University Of Alberta. Faculty Of Medicine And Oral Health Sciences; CanadáFil: Alvarez, Bernardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigaciones Cardiovasculares ; ArgentinaLippincott Williams2017-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/49489Aiello, Ernesto Alejandro; Casey, Joseph R.; Alvarez, Bernardo; Cl−/HCO3− Exchanger slc26a6: a ph regulator shapes the cardiac action potential; Lippincott Williams; Circulation-arrhythmia And Electrophysiology; 10; 10; 10-2017; 1-31941-3149CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://circep.ahajournals.org/content/10/10/e005812.longinfo:eu-repo/semantics/altIdentifier/doi/10.1161/CIRCEP.117.005812info:eu-repo/semantics/altIdentifier/pmid/29025770info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:41:07Zoai:ri.conicet.gov.ar:11336/49489instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:41:07.364CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cl−/HCO3− Exchanger slc26a6: a ph regulator shapes the cardiac action potential
title Cl−/HCO3− Exchanger slc26a6: a ph regulator shapes the cardiac action potential
spellingShingle Cl−/HCO3− Exchanger slc26a6: a ph regulator shapes the cardiac action potential
Aiello, Ernesto Alejandro
Action Potentials
Ion Transport
Physiology
Sarcoplasmatic Reticulum
title_short Cl−/HCO3− Exchanger slc26a6: a ph regulator shapes the cardiac action potential
title_full Cl−/HCO3− Exchanger slc26a6: a ph regulator shapes the cardiac action potential
title_fullStr Cl−/HCO3− Exchanger slc26a6: a ph regulator shapes the cardiac action potential
title_full_unstemmed Cl−/HCO3− Exchanger slc26a6: a ph regulator shapes the cardiac action potential
title_sort Cl−/HCO3− Exchanger slc26a6: a ph regulator shapes the cardiac action potential
dc.creator.none.fl_str_mv Aiello, Ernesto Alejandro
Casey, Joseph R.
Alvarez, Bernardo
author Aiello, Ernesto Alejandro
author_facet Aiello, Ernesto Alejandro
Casey, Joseph R.
Alvarez, Bernardo
author_role author
author2 Casey, Joseph R.
Alvarez, Bernardo
author2_role author
author
dc.subject.none.fl_str_mv Action Potentials
Ion Transport
Physiology
Sarcoplasmatic Reticulum
topic Action Potentials
Ion Transport
Physiology
Sarcoplasmatic Reticulum
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Regulation of intracellular pH (pHi) is often considered a ?housekeeping? function, contributing little to cardiac function. With the study published by Sirish et al7 in this issue of Circulation: Arrhythmia and Electrophysiology, a Cl-/HCO3- exchanger is revealed to have a more central role in cardiac function.Intracellular pH is an important modulator of cardiac excitation and contraction1, and adversely contributes to electrical arrhythmia2. Cardiac myocytes express a complex apparatus to regulate pHi. Cardiac muscle cytosolic pH (~7.2) is maintained by sarcolemmal ion transport proteins that move H+, OH− or HCO3− ions across the membrane3. Along with the acid extruders Na+/H+ exchanger (NHE1) and Na+/HCO3− cotransporter (NBC, electrogenic NBCe1/e2 and electroneutral NBCn1) myocytes possess Cl-/HCO3− exchangers (SLC4 family members AE1, AE2, and AE3) and Cl-/OH- exchanger (CHE, with no molecular identity) alkali extruders3. SLC26 gene family members were identified as (mouse slc26a64 and its human orthologue SLC26A65, and slc26a36) responsible for Cl-/HCO3− and Cl-/OH- at plasma membrane of heart ventricles6. The work of Sirish et al7 in this issue revealed that ablation of slc26a6, a plasma membrane Cl-/HCO3- exchange protein, results in cardiac action potential (AP) shortening, cardiomyocyte Ca2+ transient (CaT) and sarcoplasmic reticulum (SR) Ca2+ load reduction, cardiomyocyte diminution of sarcomeric shortening, and cardiomyocyte intracellular pH (pHi) elevation. Moreover, in slc26a6-/- mice these factors led to a reduction of cardiac fractional shortening and cardiac contractility responses, and alterations of cardiac conduction system, as seen in sinus bradycardia and fragmentation of the QRS electrocardiographic-recorded complex. Since slc26a6 has stoichiometry of 2 (or more) HCO3-: Cl-, its transport function is electrogenic, with significance to the cardiomyocyte membrane potential.
Fil: Aiello, Ernesto Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigaciones Cardiovasculares ; Argentina
Fil: Casey, Joseph R.. University Of Alberta. Faculty Of Medicine And Oral Health Sciences; Canadá
Fil: Alvarez, Bernardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigaciones Cardiovasculares ; Argentina
description Regulation of intracellular pH (pHi) is often considered a ?housekeeping? function, contributing little to cardiac function. With the study published by Sirish et al7 in this issue of Circulation: Arrhythmia and Electrophysiology, a Cl-/HCO3- exchanger is revealed to have a more central role in cardiac function.Intracellular pH is an important modulator of cardiac excitation and contraction1, and adversely contributes to electrical arrhythmia2. Cardiac myocytes express a complex apparatus to regulate pHi. Cardiac muscle cytosolic pH (~7.2) is maintained by sarcolemmal ion transport proteins that move H+, OH− or HCO3− ions across the membrane3. Along with the acid extruders Na+/H+ exchanger (NHE1) and Na+/HCO3− cotransporter (NBC, electrogenic NBCe1/e2 and electroneutral NBCn1) myocytes possess Cl-/HCO3− exchangers (SLC4 family members AE1, AE2, and AE3) and Cl-/OH- exchanger (CHE, with no molecular identity) alkali extruders3. SLC26 gene family members were identified as (mouse slc26a64 and its human orthologue SLC26A65, and slc26a36) responsible for Cl-/HCO3− and Cl-/OH- at plasma membrane of heart ventricles6. The work of Sirish et al7 in this issue revealed that ablation of slc26a6, a plasma membrane Cl-/HCO3- exchange protein, results in cardiac action potential (AP) shortening, cardiomyocyte Ca2+ transient (CaT) and sarcoplasmic reticulum (SR) Ca2+ load reduction, cardiomyocyte diminution of sarcomeric shortening, and cardiomyocyte intracellular pH (pHi) elevation. Moreover, in slc26a6-/- mice these factors led to a reduction of cardiac fractional shortening and cardiac contractility responses, and alterations of cardiac conduction system, as seen in sinus bradycardia and fragmentation of the QRS electrocardiographic-recorded complex. Since slc26a6 has stoichiometry of 2 (or more) HCO3-: Cl-, its transport function is electrogenic, with significance to the cardiomyocyte membrane potential.
publishDate 2017
dc.date.none.fl_str_mv 2017-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/49489
Aiello, Ernesto Alejandro; Casey, Joseph R.; Alvarez, Bernardo; Cl−/HCO3− Exchanger slc26a6: a ph regulator shapes the cardiac action potential; Lippincott Williams; Circulation-arrhythmia And Electrophysiology; 10; 10; 10-2017; 1-3
1941-3149
CONICET Digital
CONICET
url http://hdl.handle.net/11336/49489
identifier_str_mv Aiello, Ernesto Alejandro; Casey, Joseph R.; Alvarez, Bernardo; Cl−/HCO3− Exchanger slc26a6: a ph regulator shapes the cardiac action potential; Lippincott Williams; Circulation-arrhythmia And Electrophysiology; 10; 10; 10-2017; 1-3
1941-3149
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://circep.ahajournals.org/content/10/10/e005812.long
info:eu-repo/semantics/altIdentifier/doi/10.1161/CIRCEP.117.005812
info:eu-repo/semantics/altIdentifier/pmid/29025770
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Lippincott Williams
publisher.none.fl_str_mv Lippincott Williams
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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