Minimally invasive system to reliably characterize ventricular electrophysiology from living donors

Autores
Oliván Viguera, Aida; Pérez Zabalza, María; García Mendívil, Laura; Mountris, Konstantinos A.; Orós Rodrigo, Sofía; Ramos Marquès, Estel; Vallejo Gil, José María; Fresneda Roldán, Pedro Carlos; Fañanás Mastral, Javier; Vázquez Sancho, Manuel; Matamala Adell, Marta; Sorribas Berjón, Fernando; Bellido Morales, Javier André; Mancebón Sierra, Francisco Javier; Vaca Núñez, Alexánder Sebastián; Ballester Cuenca, Carlos; Marigil, Miguel Ángel; Pastor, Cristina; Ordovás, Laura; Köhler, Ralf; Diez, Emiliano Raúl; Pueyo, Esther
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cardiac tissue slices preserve the heterogeneous structure and multicellularity of the myocardium and allow its functional characterization. However, access to human ventricular samples is scarce. We aim to demonstrate that slices from small transmural core biopsies collected from living donors during routine cardiac surgery preserve structural and functional properties of larger myocardial specimens, allowing accurate electrophysiological characterization. In pigs, we compared left ventricular transmural core biopsies with transmural tissue blocks from the same ventricular region. In humans, we analyzed transmural biopsies and papillary muscles from living donors. All tissues were vibratomesliced. By histological analysis of the transmural biopsies, we showed that tissue architecture and cellular organization were preserved. Enzymatic and vital staining methods verifed viability. Optically mapped transmembrane potentials confrmed that action potential duration and morphology were similar in pig biopsies and tissue blocks. Action potential morphology and duration in human biopsies and papillary muscles agreed with published ranges. In both pigs and humans, responses to increasing pacing frequencies and β-adrenergic stimulation were similar in transmural biopsies and larger tissues. We show that it is possible to successfully collect and characterize tissue slices from human myocardial biopsies routinely extracted from living donors, whose behavior mimics that of larger myocardial preparations both structurally and electrophysiologically.
Fil: Oliván Viguera, Aida. Universidad de Zaragoza; España
Fil: Pérez Zabalza, María. Universidad de Zaragoza; España
Fil: García Mendívil, Laura. Universidad de Zaragoza; España
Fil: Mountris, Konstantinos A.. Universidad de Zaragoza; España
Fil: Orós Rodrigo, Sofía. Universidad de Zaragoza; España
Fil: Ramos Marquès, Estel. Universidad de Zaragoza; España
Fil: Vallejo Gil, José María. University Hospital Miguel Servet; España
Fil: Fresneda Roldán, Pedro Carlos. University Hospital Miguel Servet; España
Fil: Fañanás Mastral, Javier. University Hospital Miguel Servet; España
Fil: Vázquez Sancho, Manuel. University Hospital Miguel Servet; España
Fil: Matamala Adell, Marta. University Hospital Miguel Servet; España
Fil: Sorribas Berjón, Fernando. University Hospital Miguel Servet; España
Fil: Bellido Morales, Javier André. University Hospital Miguel Servet; España
Fil: Mancebón Sierra, Francisco Javier. University Hospital Miguel Servet; España
Fil: Vaca Núñez, Alexánder Sebastián. University Hospital Miguel Servet; España
Fil: Ballester Cuenca, Carlos. University Hospital Miguel Servet; España
Fil: Marigil, Miguel Ángel. Hospital San Jorge; España
Fil: Pastor, Cristina. Aragón Institute of Health Sciences; España
Fil: Ordovás, Laura. Aragón Agency for Research and Development; España. Universidad de Zaragoza; España
Fil: Köhler, Ralf. Aragón Institute of Health Sciences; España. Aragón Agency for Research and Development; España
Fil: Diez, Emiliano Raúl. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Cátedra de Fisiología Humana Normal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Pueyo, Esther. Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina; España. Universidad de Zaragoza; España
Materia
MYOCARDIAL SLICE
ELECTROPHYSIOLOGY
LIVING DONORS
ACTION POTENTIALS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/142132

id CONICETDig_a4538d119ed6fa6a4667731ed3656ee3
oai_identifier_str oai:ri.conicet.gov.ar:11336/142132
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Minimally invasive system to reliably characterize ventricular electrophysiology from living donorsOliván Viguera, AidaPérez Zabalza, MaríaGarcía Mendívil, LauraMountris, Konstantinos A.Orós Rodrigo, SofíaRamos Marquès, EstelVallejo Gil, José MaríaFresneda Roldán, Pedro CarlosFañanás Mastral, JavierVázquez Sancho, ManuelMatamala Adell, MartaSorribas Berjón, FernandoBellido Morales, Javier AndréMancebón Sierra, Francisco JavierVaca Núñez, Alexánder SebastiánBallester Cuenca, CarlosMarigil, Miguel ÁngelPastor, CristinaOrdovás, LauraKöhler, RalfDiez, Emiliano RaúlPueyo, EstherMYOCARDIAL SLICEELECTROPHYSIOLOGYLIVING DONORSACTION POTENTIALShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Cardiac tissue slices preserve the heterogeneous structure and multicellularity of the myocardium and allow its functional characterization. However, access to human ventricular samples is scarce. We aim to demonstrate that slices from small transmural core biopsies collected from living donors during routine cardiac surgery preserve structural and functional properties of larger myocardial specimens, allowing accurate electrophysiological characterization. In pigs, we compared left ventricular transmural core biopsies with transmural tissue blocks from the same ventricular region. In humans, we analyzed transmural biopsies and papillary muscles from living donors. All tissues were vibratomesliced. By histological analysis of the transmural biopsies, we showed that tissue architecture and cellular organization were preserved. Enzymatic and vital staining methods verifed viability. Optically mapped transmembrane potentials confrmed that action potential duration and morphology were similar in pig biopsies and tissue blocks. Action potential morphology and duration in human biopsies and papillary muscles agreed with published ranges. In both pigs and humans, responses to increasing pacing frequencies and β-adrenergic stimulation were similar in transmural biopsies and larger tissues. We show that it is possible to successfully collect and characterize tissue slices from human myocardial biopsies routinely extracted from living donors, whose behavior mimics that of larger myocardial preparations both structurally and electrophysiologically.Fil: Oliván Viguera, Aida. Universidad de Zaragoza; EspañaFil: Pérez Zabalza, María. Universidad de Zaragoza; EspañaFil: García Mendívil, Laura. Universidad de Zaragoza; EspañaFil: Mountris, Konstantinos A.. Universidad de Zaragoza; EspañaFil: Orós Rodrigo, Sofía. Universidad de Zaragoza; EspañaFil: Ramos Marquès, Estel. Universidad de Zaragoza; EspañaFil: Vallejo Gil, José María. University Hospital Miguel Servet; EspañaFil: Fresneda Roldán, Pedro Carlos. University Hospital Miguel Servet; EspañaFil: Fañanás Mastral, Javier. University Hospital Miguel Servet; EspañaFil: Vázquez Sancho, Manuel. University Hospital Miguel Servet; EspañaFil: Matamala Adell, Marta. University Hospital Miguel Servet; EspañaFil: Sorribas Berjón, Fernando. University Hospital Miguel Servet; EspañaFil: Bellido Morales, Javier André. University Hospital Miguel Servet; EspañaFil: Mancebón Sierra, Francisco Javier. University Hospital Miguel Servet; EspañaFil: Vaca Núñez, Alexánder Sebastián. University Hospital Miguel Servet; EspañaFil: Ballester Cuenca, Carlos. University Hospital Miguel Servet; EspañaFil: Marigil, Miguel Ángel. Hospital San Jorge; EspañaFil: Pastor, Cristina. Aragón Institute of Health Sciences; EspañaFil: Ordovás, Laura. Aragón Agency for Research and Development; España. Universidad de Zaragoza; EspañaFil: Köhler, Ralf. Aragón Institute of Health Sciences; España. Aragón Agency for Research and Development; EspañaFil: Diez, Emiliano Raúl. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Cátedra de Fisiología Humana Normal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Pueyo, Esther. Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina; España. Universidad de Zaragoza; EspañaNature Research2020-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/142132Oliván Viguera, Aida; Pérez Zabalza, María; García Mendívil, Laura; Mountris, Konstantinos A.; Orós Rodrigo, Sofía; et al.; Minimally invasive system to reliably characterize ventricular electrophysiology from living donors; Nature Research; Scientific Reports; 10; 1; 11-2020; 1-132045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.nature.com/articles/s41598-020-77076-0info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-020-77076-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:33:05Zoai:ri.conicet.gov.ar:11336/142132instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:33:05.952CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Minimally invasive system to reliably characterize ventricular electrophysiology from living donors
title Minimally invasive system to reliably characterize ventricular electrophysiology from living donors
spellingShingle Minimally invasive system to reliably characterize ventricular electrophysiology from living donors
Oliván Viguera, Aida
MYOCARDIAL SLICE
ELECTROPHYSIOLOGY
LIVING DONORS
ACTION POTENTIALS
title_short Minimally invasive system to reliably characterize ventricular electrophysiology from living donors
title_full Minimally invasive system to reliably characterize ventricular electrophysiology from living donors
title_fullStr Minimally invasive system to reliably characterize ventricular electrophysiology from living donors
title_full_unstemmed Minimally invasive system to reliably characterize ventricular electrophysiology from living donors
title_sort Minimally invasive system to reliably characterize ventricular electrophysiology from living donors
dc.creator.none.fl_str_mv Oliván Viguera, Aida
Pérez Zabalza, María
García Mendívil, Laura
Mountris, Konstantinos A.
Orós Rodrigo, Sofía
Ramos Marquès, Estel
Vallejo Gil, José María
Fresneda Roldán, Pedro Carlos
Fañanás Mastral, Javier
Vázquez Sancho, Manuel
Matamala Adell, Marta
Sorribas Berjón, Fernando
Bellido Morales, Javier André
Mancebón Sierra, Francisco Javier
Vaca Núñez, Alexánder Sebastián
Ballester Cuenca, Carlos
Marigil, Miguel Ángel
Pastor, Cristina
Ordovás, Laura
Köhler, Ralf
Diez, Emiliano Raúl
Pueyo, Esther
author Oliván Viguera, Aida
author_facet Oliván Viguera, Aida
Pérez Zabalza, María
García Mendívil, Laura
Mountris, Konstantinos A.
Orós Rodrigo, Sofía
Ramos Marquès, Estel
Vallejo Gil, José María
Fresneda Roldán, Pedro Carlos
Fañanás Mastral, Javier
Vázquez Sancho, Manuel
Matamala Adell, Marta
Sorribas Berjón, Fernando
Bellido Morales, Javier André
Mancebón Sierra, Francisco Javier
Vaca Núñez, Alexánder Sebastián
Ballester Cuenca, Carlos
Marigil, Miguel Ángel
Pastor, Cristina
Ordovás, Laura
Köhler, Ralf
Diez, Emiliano Raúl
Pueyo, Esther
author_role author
author2 Pérez Zabalza, María
García Mendívil, Laura
Mountris, Konstantinos A.
Orós Rodrigo, Sofía
Ramos Marquès, Estel
Vallejo Gil, José María
Fresneda Roldán, Pedro Carlos
Fañanás Mastral, Javier
Vázquez Sancho, Manuel
Matamala Adell, Marta
Sorribas Berjón, Fernando
Bellido Morales, Javier André
Mancebón Sierra, Francisco Javier
Vaca Núñez, Alexánder Sebastián
Ballester Cuenca, Carlos
Marigil, Miguel Ángel
Pastor, Cristina
Ordovás, Laura
Köhler, Ralf
Diez, Emiliano Raúl
Pueyo, Esther
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv MYOCARDIAL SLICE
ELECTROPHYSIOLOGY
LIVING DONORS
ACTION POTENTIALS
topic MYOCARDIAL SLICE
ELECTROPHYSIOLOGY
LIVING DONORS
ACTION POTENTIALS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Cardiac tissue slices preserve the heterogeneous structure and multicellularity of the myocardium and allow its functional characterization. However, access to human ventricular samples is scarce. We aim to demonstrate that slices from small transmural core biopsies collected from living donors during routine cardiac surgery preserve structural and functional properties of larger myocardial specimens, allowing accurate electrophysiological characterization. In pigs, we compared left ventricular transmural core biopsies with transmural tissue blocks from the same ventricular region. In humans, we analyzed transmural biopsies and papillary muscles from living donors. All tissues were vibratomesliced. By histological analysis of the transmural biopsies, we showed that tissue architecture and cellular organization were preserved. Enzymatic and vital staining methods verifed viability. Optically mapped transmembrane potentials confrmed that action potential duration and morphology were similar in pig biopsies and tissue blocks. Action potential morphology and duration in human biopsies and papillary muscles agreed with published ranges. In both pigs and humans, responses to increasing pacing frequencies and β-adrenergic stimulation were similar in transmural biopsies and larger tissues. We show that it is possible to successfully collect and characterize tissue slices from human myocardial biopsies routinely extracted from living donors, whose behavior mimics that of larger myocardial preparations both structurally and electrophysiologically.
Fil: Oliván Viguera, Aida. Universidad de Zaragoza; España
Fil: Pérez Zabalza, María. Universidad de Zaragoza; España
Fil: García Mendívil, Laura. Universidad de Zaragoza; España
Fil: Mountris, Konstantinos A.. Universidad de Zaragoza; España
Fil: Orós Rodrigo, Sofía. Universidad de Zaragoza; España
Fil: Ramos Marquès, Estel. Universidad de Zaragoza; España
Fil: Vallejo Gil, José María. University Hospital Miguel Servet; España
Fil: Fresneda Roldán, Pedro Carlos. University Hospital Miguel Servet; España
Fil: Fañanás Mastral, Javier. University Hospital Miguel Servet; España
Fil: Vázquez Sancho, Manuel. University Hospital Miguel Servet; España
Fil: Matamala Adell, Marta. University Hospital Miguel Servet; España
Fil: Sorribas Berjón, Fernando. University Hospital Miguel Servet; España
Fil: Bellido Morales, Javier André. University Hospital Miguel Servet; España
Fil: Mancebón Sierra, Francisco Javier. University Hospital Miguel Servet; España
Fil: Vaca Núñez, Alexánder Sebastián. University Hospital Miguel Servet; España
Fil: Ballester Cuenca, Carlos. University Hospital Miguel Servet; España
Fil: Marigil, Miguel Ángel. Hospital San Jorge; España
Fil: Pastor, Cristina. Aragón Institute of Health Sciences; España
Fil: Ordovás, Laura. Aragón Agency for Research and Development; España. Universidad de Zaragoza; España
Fil: Köhler, Ralf. Aragón Institute of Health Sciences; España. Aragón Agency for Research and Development; España
Fil: Diez, Emiliano Raúl. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Cátedra de Fisiología Humana Normal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Pueyo, Esther. Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina; España. Universidad de Zaragoza; España
description Cardiac tissue slices preserve the heterogeneous structure and multicellularity of the myocardium and allow its functional characterization. However, access to human ventricular samples is scarce. We aim to demonstrate that slices from small transmural core biopsies collected from living donors during routine cardiac surgery preserve structural and functional properties of larger myocardial specimens, allowing accurate electrophysiological characterization. In pigs, we compared left ventricular transmural core biopsies with transmural tissue blocks from the same ventricular region. In humans, we analyzed transmural biopsies and papillary muscles from living donors. All tissues were vibratomesliced. By histological analysis of the transmural biopsies, we showed that tissue architecture and cellular organization were preserved. Enzymatic and vital staining methods verifed viability. Optically mapped transmembrane potentials confrmed that action potential duration and morphology were similar in pig biopsies and tissue blocks. Action potential morphology and duration in human biopsies and papillary muscles agreed with published ranges. In both pigs and humans, responses to increasing pacing frequencies and β-adrenergic stimulation were similar in transmural biopsies and larger tissues. We show that it is possible to successfully collect and characterize tissue slices from human myocardial biopsies routinely extracted from living donors, whose behavior mimics that of larger myocardial preparations both structurally and electrophysiologically.
publishDate 2020
dc.date.none.fl_str_mv 2020-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/142132
Oliván Viguera, Aida; Pérez Zabalza, María; García Mendívil, Laura; Mountris, Konstantinos A.; Orós Rodrigo, Sofía; et al.; Minimally invasive system to reliably characterize ventricular electrophysiology from living donors; Nature Research; Scientific Reports; 10; 1; 11-2020; 1-13
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/142132
identifier_str_mv Oliván Viguera, Aida; Pérez Zabalza, María; García Mendívil, Laura; Mountris, Konstantinos A.; Orós Rodrigo, Sofía; et al.; Minimally invasive system to reliably characterize ventricular electrophysiology from living donors; Nature Research; Scientific Reports; 10; 1; 11-2020; 1-13
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.nature.com/articles/s41598-020-77076-0
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-020-77076-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Research
publisher.none.fl_str_mv Nature Research
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614346083139584
score 13.070432