Minimally invasive system to reliably characterize ventricular electrophysiology from living donors
- Autores
- Oliván Viguera, Aida; Pérez Zabalza, María; García Mendívil, Laura; Mountris, Konstantinos A.; Orós Rodrigo, Sofía; Ramos Marquès, Estel; Vallejo Gil, José María; Fresneda Roldán, Pedro Carlos; Fañanás Mastral, Javier; Vázquez Sancho, Manuel; Matamala Adell, Marta; Sorribas Berjón, Fernando; Bellido Morales, Javier André; Mancebón Sierra, Francisco Javier; Vaca Núñez, Alexánder Sebastián; Ballester Cuenca, Carlos; Marigil, Miguel Ángel; Pastor, Cristina; Ordovás, Laura; Köhler, Ralf; Diez, Emiliano Raúl; Pueyo, Esther
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Cardiac tissue slices preserve the heterogeneous structure and multicellularity of the myocardium and allow its functional characterization. However, access to human ventricular samples is scarce. We aim to demonstrate that slices from small transmural core biopsies collected from living donors during routine cardiac surgery preserve structural and functional properties of larger myocardial specimens, allowing accurate electrophysiological characterization. In pigs, we compared left ventricular transmural core biopsies with transmural tissue blocks from the same ventricular region. In humans, we analyzed transmural biopsies and papillary muscles from living donors. All tissues were vibratomesliced. By histological analysis of the transmural biopsies, we showed that tissue architecture and cellular organization were preserved. Enzymatic and vital staining methods verifed viability. Optically mapped transmembrane potentials confrmed that action potential duration and morphology were similar in pig biopsies and tissue blocks. Action potential morphology and duration in human biopsies and papillary muscles agreed with published ranges. In both pigs and humans, responses to increasing pacing frequencies and β-adrenergic stimulation were similar in transmural biopsies and larger tissues. We show that it is possible to successfully collect and characterize tissue slices from human myocardial biopsies routinely extracted from living donors, whose behavior mimics that of larger myocardial preparations both structurally and electrophysiologically.
Fil: Oliván Viguera, Aida. Universidad de Zaragoza; España
Fil: Pérez Zabalza, María. Universidad de Zaragoza; España
Fil: García Mendívil, Laura. Universidad de Zaragoza; España
Fil: Mountris, Konstantinos A.. Universidad de Zaragoza; España
Fil: Orós Rodrigo, Sofía. Universidad de Zaragoza; España
Fil: Ramos Marquès, Estel. Universidad de Zaragoza; España
Fil: Vallejo Gil, José María. University Hospital Miguel Servet; España
Fil: Fresneda Roldán, Pedro Carlos. University Hospital Miguel Servet; España
Fil: Fañanás Mastral, Javier. University Hospital Miguel Servet; España
Fil: Vázquez Sancho, Manuel. University Hospital Miguel Servet; España
Fil: Matamala Adell, Marta. University Hospital Miguel Servet; España
Fil: Sorribas Berjón, Fernando. University Hospital Miguel Servet; España
Fil: Bellido Morales, Javier André. University Hospital Miguel Servet; España
Fil: Mancebón Sierra, Francisco Javier. University Hospital Miguel Servet; España
Fil: Vaca Núñez, Alexánder Sebastián. University Hospital Miguel Servet; España
Fil: Ballester Cuenca, Carlos. University Hospital Miguel Servet; España
Fil: Marigil, Miguel Ángel. Hospital San Jorge; España
Fil: Pastor, Cristina. Aragón Institute of Health Sciences; España
Fil: Ordovás, Laura. Aragón Agency for Research and Development; España. Universidad de Zaragoza; España
Fil: Köhler, Ralf. Aragón Institute of Health Sciences; España. Aragón Agency for Research and Development; España
Fil: Diez, Emiliano Raúl. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Cátedra de Fisiología Humana Normal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Pueyo, Esther. Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina; España. Universidad de Zaragoza; España - Materia
-
MYOCARDIAL SLICE
ELECTROPHYSIOLOGY
LIVING DONORS
ACTION POTENTIALS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/142132
Ver los metadatos del registro completo
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oai:ri.conicet.gov.ar:11336/142132 |
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CONICET Digital (CONICET) |
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Minimally invasive system to reliably characterize ventricular electrophysiology from living donorsOliván Viguera, AidaPérez Zabalza, MaríaGarcía Mendívil, LauraMountris, Konstantinos A.Orós Rodrigo, SofíaRamos Marquès, EstelVallejo Gil, José MaríaFresneda Roldán, Pedro CarlosFañanás Mastral, JavierVázquez Sancho, ManuelMatamala Adell, MartaSorribas Berjón, FernandoBellido Morales, Javier AndréMancebón Sierra, Francisco JavierVaca Núñez, Alexánder SebastiánBallester Cuenca, CarlosMarigil, Miguel ÁngelPastor, CristinaOrdovás, LauraKöhler, RalfDiez, Emiliano RaúlPueyo, EstherMYOCARDIAL SLICEELECTROPHYSIOLOGYLIVING DONORSACTION POTENTIALShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Cardiac tissue slices preserve the heterogeneous structure and multicellularity of the myocardium and allow its functional characterization. However, access to human ventricular samples is scarce. We aim to demonstrate that slices from small transmural core biopsies collected from living donors during routine cardiac surgery preserve structural and functional properties of larger myocardial specimens, allowing accurate electrophysiological characterization. In pigs, we compared left ventricular transmural core biopsies with transmural tissue blocks from the same ventricular region. In humans, we analyzed transmural biopsies and papillary muscles from living donors. All tissues were vibratomesliced. By histological analysis of the transmural biopsies, we showed that tissue architecture and cellular organization were preserved. Enzymatic and vital staining methods verifed viability. Optically mapped transmembrane potentials confrmed that action potential duration and morphology were similar in pig biopsies and tissue blocks. Action potential morphology and duration in human biopsies and papillary muscles agreed with published ranges. In both pigs and humans, responses to increasing pacing frequencies and β-adrenergic stimulation were similar in transmural biopsies and larger tissues. We show that it is possible to successfully collect and characterize tissue slices from human myocardial biopsies routinely extracted from living donors, whose behavior mimics that of larger myocardial preparations both structurally and electrophysiologically.Fil: Oliván Viguera, Aida. Universidad de Zaragoza; EspañaFil: Pérez Zabalza, María. Universidad de Zaragoza; EspañaFil: García Mendívil, Laura. Universidad de Zaragoza; EspañaFil: Mountris, Konstantinos A.. Universidad de Zaragoza; EspañaFil: Orós Rodrigo, Sofía. Universidad de Zaragoza; EspañaFil: Ramos Marquès, Estel. Universidad de Zaragoza; EspañaFil: Vallejo Gil, José María. University Hospital Miguel Servet; EspañaFil: Fresneda Roldán, Pedro Carlos. University Hospital Miguel Servet; EspañaFil: Fañanás Mastral, Javier. University Hospital Miguel Servet; EspañaFil: Vázquez Sancho, Manuel. University Hospital Miguel Servet; EspañaFil: Matamala Adell, Marta. University Hospital Miguel Servet; EspañaFil: Sorribas Berjón, Fernando. University Hospital Miguel Servet; EspañaFil: Bellido Morales, Javier André. University Hospital Miguel Servet; EspañaFil: Mancebón Sierra, Francisco Javier. University Hospital Miguel Servet; EspañaFil: Vaca Núñez, Alexánder Sebastián. University Hospital Miguel Servet; EspañaFil: Ballester Cuenca, Carlos. University Hospital Miguel Servet; EspañaFil: Marigil, Miguel Ángel. Hospital San Jorge; EspañaFil: Pastor, Cristina. Aragón Institute of Health Sciences; EspañaFil: Ordovás, Laura. Aragón Agency for Research and Development; España. Universidad de Zaragoza; EspañaFil: Köhler, Ralf. Aragón Institute of Health Sciences; España. Aragón Agency for Research and Development; EspañaFil: Diez, Emiliano Raúl. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Cátedra de Fisiología Humana Normal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Pueyo, Esther. Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina; España. Universidad de Zaragoza; EspañaNature Research2020-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/142132Oliván Viguera, Aida; Pérez Zabalza, María; García Mendívil, Laura; Mountris, Konstantinos A.; Orós Rodrigo, Sofía; et al.; Minimally invasive system to reliably characterize ventricular electrophysiology from living donors; Nature Research; Scientific Reports; 10; 1; 11-2020; 1-132045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.nature.com/articles/s41598-020-77076-0info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-020-77076-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:33:05Zoai:ri.conicet.gov.ar:11336/142132instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:33:05.952CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Minimally invasive system to reliably characterize ventricular electrophysiology from living donors |
title |
Minimally invasive system to reliably characterize ventricular electrophysiology from living donors |
spellingShingle |
Minimally invasive system to reliably characterize ventricular electrophysiology from living donors Oliván Viguera, Aida MYOCARDIAL SLICE ELECTROPHYSIOLOGY LIVING DONORS ACTION POTENTIALS |
title_short |
Minimally invasive system to reliably characterize ventricular electrophysiology from living donors |
title_full |
Minimally invasive system to reliably characterize ventricular electrophysiology from living donors |
title_fullStr |
Minimally invasive system to reliably characterize ventricular electrophysiology from living donors |
title_full_unstemmed |
Minimally invasive system to reliably characterize ventricular electrophysiology from living donors |
title_sort |
Minimally invasive system to reliably characterize ventricular electrophysiology from living donors |
dc.creator.none.fl_str_mv |
Oliván Viguera, Aida Pérez Zabalza, María García Mendívil, Laura Mountris, Konstantinos A. Orós Rodrigo, Sofía Ramos Marquès, Estel Vallejo Gil, José María Fresneda Roldán, Pedro Carlos Fañanás Mastral, Javier Vázquez Sancho, Manuel Matamala Adell, Marta Sorribas Berjón, Fernando Bellido Morales, Javier André Mancebón Sierra, Francisco Javier Vaca Núñez, Alexánder Sebastián Ballester Cuenca, Carlos Marigil, Miguel Ángel Pastor, Cristina Ordovás, Laura Köhler, Ralf Diez, Emiliano Raúl Pueyo, Esther |
author |
Oliván Viguera, Aida |
author_facet |
Oliván Viguera, Aida Pérez Zabalza, María García Mendívil, Laura Mountris, Konstantinos A. Orós Rodrigo, Sofía Ramos Marquès, Estel Vallejo Gil, José María Fresneda Roldán, Pedro Carlos Fañanás Mastral, Javier Vázquez Sancho, Manuel Matamala Adell, Marta Sorribas Berjón, Fernando Bellido Morales, Javier André Mancebón Sierra, Francisco Javier Vaca Núñez, Alexánder Sebastián Ballester Cuenca, Carlos Marigil, Miguel Ángel Pastor, Cristina Ordovás, Laura Köhler, Ralf Diez, Emiliano Raúl Pueyo, Esther |
author_role |
author |
author2 |
Pérez Zabalza, María García Mendívil, Laura Mountris, Konstantinos A. Orós Rodrigo, Sofía Ramos Marquès, Estel Vallejo Gil, José María Fresneda Roldán, Pedro Carlos Fañanás Mastral, Javier Vázquez Sancho, Manuel Matamala Adell, Marta Sorribas Berjón, Fernando Bellido Morales, Javier André Mancebón Sierra, Francisco Javier Vaca Núñez, Alexánder Sebastián Ballester Cuenca, Carlos Marigil, Miguel Ángel Pastor, Cristina Ordovás, Laura Köhler, Ralf Diez, Emiliano Raúl Pueyo, Esther |
author2_role |
author author author author author author author author author author author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
MYOCARDIAL SLICE ELECTROPHYSIOLOGY LIVING DONORS ACTION POTENTIALS |
topic |
MYOCARDIAL SLICE ELECTROPHYSIOLOGY LIVING DONORS ACTION POTENTIALS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Cardiac tissue slices preserve the heterogeneous structure and multicellularity of the myocardium and allow its functional characterization. However, access to human ventricular samples is scarce. We aim to demonstrate that slices from small transmural core biopsies collected from living donors during routine cardiac surgery preserve structural and functional properties of larger myocardial specimens, allowing accurate electrophysiological characterization. In pigs, we compared left ventricular transmural core biopsies with transmural tissue blocks from the same ventricular region. In humans, we analyzed transmural biopsies and papillary muscles from living donors. All tissues were vibratomesliced. By histological analysis of the transmural biopsies, we showed that tissue architecture and cellular organization were preserved. Enzymatic and vital staining methods verifed viability. Optically mapped transmembrane potentials confrmed that action potential duration and morphology were similar in pig biopsies and tissue blocks. Action potential morphology and duration in human biopsies and papillary muscles agreed with published ranges. In both pigs and humans, responses to increasing pacing frequencies and β-adrenergic stimulation were similar in transmural biopsies and larger tissues. We show that it is possible to successfully collect and characterize tissue slices from human myocardial biopsies routinely extracted from living donors, whose behavior mimics that of larger myocardial preparations both structurally and electrophysiologically. Fil: Oliván Viguera, Aida. Universidad de Zaragoza; España Fil: Pérez Zabalza, María. Universidad de Zaragoza; España Fil: García Mendívil, Laura. Universidad de Zaragoza; España Fil: Mountris, Konstantinos A.. Universidad de Zaragoza; España Fil: Orós Rodrigo, Sofía. Universidad de Zaragoza; España Fil: Ramos Marquès, Estel. Universidad de Zaragoza; España Fil: Vallejo Gil, José María. University Hospital Miguel Servet; España Fil: Fresneda Roldán, Pedro Carlos. University Hospital Miguel Servet; España Fil: Fañanás Mastral, Javier. University Hospital Miguel Servet; España Fil: Vázquez Sancho, Manuel. University Hospital Miguel Servet; España Fil: Matamala Adell, Marta. University Hospital Miguel Servet; España Fil: Sorribas Berjón, Fernando. University Hospital Miguel Servet; España Fil: Bellido Morales, Javier André. University Hospital Miguel Servet; España Fil: Mancebón Sierra, Francisco Javier. University Hospital Miguel Servet; España Fil: Vaca Núñez, Alexánder Sebastián. University Hospital Miguel Servet; España Fil: Ballester Cuenca, Carlos. University Hospital Miguel Servet; España Fil: Marigil, Miguel Ángel. Hospital San Jorge; España Fil: Pastor, Cristina. Aragón Institute of Health Sciences; España Fil: Ordovás, Laura. Aragón Agency for Research and Development; España. Universidad de Zaragoza; España Fil: Köhler, Ralf. Aragón Institute of Health Sciences; España. Aragón Agency for Research and Development; España Fil: Diez, Emiliano Raúl. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Cátedra de Fisiología Humana Normal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina Fil: Pueyo, Esther. Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina; España. Universidad de Zaragoza; España |
description |
Cardiac tissue slices preserve the heterogeneous structure and multicellularity of the myocardium and allow its functional characterization. However, access to human ventricular samples is scarce. We aim to demonstrate that slices from small transmural core biopsies collected from living donors during routine cardiac surgery preserve structural and functional properties of larger myocardial specimens, allowing accurate electrophysiological characterization. In pigs, we compared left ventricular transmural core biopsies with transmural tissue blocks from the same ventricular region. In humans, we analyzed transmural biopsies and papillary muscles from living donors. All tissues were vibratomesliced. By histological analysis of the transmural biopsies, we showed that tissue architecture and cellular organization were preserved. Enzymatic and vital staining methods verifed viability. Optically mapped transmembrane potentials confrmed that action potential duration and morphology were similar in pig biopsies and tissue blocks. Action potential morphology and duration in human biopsies and papillary muscles agreed with published ranges. In both pigs and humans, responses to increasing pacing frequencies and β-adrenergic stimulation were similar in transmural biopsies and larger tissues. We show that it is possible to successfully collect and characterize tissue slices from human myocardial biopsies routinely extracted from living donors, whose behavior mimics that of larger myocardial preparations both structurally and electrophysiologically. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-11 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/142132 Oliván Viguera, Aida; Pérez Zabalza, María; García Mendívil, Laura; Mountris, Konstantinos A.; Orós Rodrigo, Sofía; et al.; Minimally invasive system to reliably characterize ventricular electrophysiology from living donors; Nature Research; Scientific Reports; 10; 1; 11-2020; 1-13 2045-2322 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/142132 |
identifier_str_mv |
Oliván Viguera, Aida; Pérez Zabalza, María; García Mendívil, Laura; Mountris, Konstantinos A.; Orós Rodrigo, Sofía; et al.; Minimally invasive system to reliably characterize ventricular electrophysiology from living donors; Nature Research; Scientific Reports; 10; 1; 11-2020; 1-13 2045-2322 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.nature.com/articles/s41598-020-77076-0 info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-020-77076-0 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Nature Research |
publisher.none.fl_str_mv |
Nature Research |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614346083139584 |
score |
13.070432 |