Diagnostic performance of commercial IgM and IgG enzyme-linked immunoassays (ELISAs) for diagnosis of Zika virus infection

Autores
Kikuti, Mariana; Tauro, Laura Beatriz; Moreira, Patrícia S. S.; Campos, Gúbio S.; Paploski, Igor A. D.; Weaver, Scott C.; Reis, Mitermayer G.; Kitron, Uriel D.; Ribeiro, Guilherme S.
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Serologic detection of Zika virus (ZIKV) infections is challenging because of antigenic similarities among flaviviruses. Objective: To evaluate the sensitivity and specificity of commercial ZIKV IgM and IgG enzyme-linked immunoassay (ELISA) kits. Methods: We used sera from febrile patients with RT-PCR-confirmed ZIKV infection to determine sensitivity and sera from RT-PCR-confirmed dengue cases and blood donors, both of which were collected before ZIKV epidemics in Brazil (2009-2011 and 2013, respectively) to determine specificity. Results: The ZIKV IgM-ELISA positivity among RT-PCR ZIKV confirmed cases was 0.0% (0/14) and 12.5% (1/8) for acute- and convalescent-phase sera, respectively, while its specificity was 100.0% (58/58) and 98.3% (58/59) for acute- and convalescent-phase sera of dengue patients, and 100.0% (23/23) for blood donors. The ZIKV IgG-ELISA sensitivity was 100.0% (6/6) on convalescent-phase sera from RT-PCR confirmed ZIKV patients, while its specificity was 27.3% (15/55) on convalescent-phase sera from dengue patients and 45.0% (9/20) on blood donors' sera. The ZIKV IgG-ELISA specificity among dengue confirmed cases was much greater among patients with primary dengue (92.3%; 12/13), compared to secondary dengue (7.1%; 3/42). Conclusions: In a setting of endemic dengue transmission, the ZIKV IgM-ELISA had high specificity, but poor sensitivity. In contrast, the ZIKV IgG-ELISA showed low specificity, particularly for patients previously exposed to dengue infections. This suggests that this ZIKV IgM-ELISA is not useful in confirming a diagnosis of ZIKV infection in suspected patients, whereas the IgG-ELISA is more suitable for ZIKV diagnosis among travelers, who reside in areas free of flavivirus transmission, rather than for serosurveys in dengue-endemic areas.
Fil: Kikuti, Mariana. Fundación Oswaldo Cruz; Brasil. Universidade Federal da Bahia; Brasil
Fil: Tauro, Laura Beatriz. Fundación Oswaldo Cruz; Brasil. Ministerio de Salud. Instituto Nacional de Medicina Tropical; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú; Argentina
Fil: Moreira, Patrícia S. S.. Fundación Oswaldo Cruz; Brasil
Fil: Campos, Gúbio S.. Universidade Federal da Bahia; Brasil
Fil: Paploski, Igor A. D.. Fundación Oswaldo Cruz; Brasil. Universidade Federal da Bahia; Brasil
Fil: Weaver, Scott C.. Ut Medical Branch At Galveston;
Fil: Reis, Mitermayer G.. Universidade Federal da Bahia; Brasil. Fundación Oswaldo Cruz; Brasil
Fil: Kitron, Uriel D.. Fundación Oswaldo Cruz; Brasil. University of Emory; Estados Unidos
Fil: Ribeiro, Guilherme S.. Universidade Federal da Bahia; Brasil. Fundación Oswaldo Cruz; Brasil
Materia
ENZYME-LINKED IMMUNOSORBENT ASSAY
IMMUNOGLOBULIN G
IMMUNOGLOBULIN M
SENSITIVITY AND SPECIFICITY
ZIKA VIRUS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/81470
Acceder
id CONICETDig_8a826e08329f026c46c6780f26f300f1
oai_identifier_str oai:ri.conicet.gov.ar:11336/81470
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Diagnostic performance of commercial IgM and IgG enzyme-linked immunoassays (ELISAs) for diagnosis of Zika virus infectionKikuti, MarianaTauro, Laura BeatrizMoreira, Patrícia S. S.Campos, Gúbio S.Paploski, Igor A. D.Weaver, Scott C.Reis, Mitermayer G.Kitron, Uriel D.Ribeiro, Guilherme S.ENZYME-LINKED IMMUNOSORBENT ASSAYIMMUNOGLOBULIN GIMMUNOGLOBULIN MSENSITIVITY AND SPECIFICITYZIKA VIRUShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: Serologic detection of Zika virus (ZIKV) infections is challenging because of antigenic similarities among flaviviruses. Objective: To evaluate the sensitivity and specificity of commercial ZIKV IgM and IgG enzyme-linked immunoassay (ELISA) kits. Methods: We used sera from febrile patients with RT-PCR-confirmed ZIKV infection to determine sensitivity and sera from RT-PCR-confirmed dengue cases and blood donors, both of which were collected before ZIKV epidemics in Brazil (2009-2011 and 2013, respectively) to determine specificity. Results: The ZIKV IgM-ELISA positivity among RT-PCR ZIKV confirmed cases was 0.0% (0/14) and 12.5% (1/8) for acute- and convalescent-phase sera, respectively, while its specificity was 100.0% (58/58) and 98.3% (58/59) for acute- and convalescent-phase sera of dengue patients, and 100.0% (23/23) for blood donors. The ZIKV IgG-ELISA sensitivity was 100.0% (6/6) on convalescent-phase sera from RT-PCR confirmed ZIKV patients, while its specificity was 27.3% (15/55) on convalescent-phase sera from dengue patients and 45.0% (9/20) on blood donors' sera. The ZIKV IgG-ELISA specificity among dengue confirmed cases was much greater among patients with primary dengue (92.3%; 12/13), compared to secondary dengue (7.1%; 3/42). Conclusions: In a setting of endemic dengue transmission, the ZIKV IgM-ELISA had high specificity, but poor sensitivity. In contrast, the ZIKV IgG-ELISA showed low specificity, particularly for patients previously exposed to dengue infections. This suggests that this ZIKV IgM-ELISA is not useful in confirming a diagnosis of ZIKV infection in suspected patients, whereas the IgG-ELISA is more suitable for ZIKV diagnosis among travelers, who reside in areas free of flavivirus transmission, rather than for serosurveys in dengue-endemic areas.Fil: Kikuti, Mariana. Fundación Oswaldo Cruz; Brasil. Universidade Federal da Bahia; BrasilFil: Tauro, Laura Beatriz. Fundación Oswaldo Cruz; Brasil. Ministerio de Salud. Instituto Nacional de Medicina Tropical; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú; ArgentinaFil: Moreira, Patrícia S. S.. Fundación Oswaldo Cruz; BrasilFil: Campos, Gúbio S.. Universidade Federal da Bahia; BrasilFil: Paploski, Igor A. D.. Fundación Oswaldo Cruz; Brasil. Universidade Federal da Bahia; BrasilFil: Weaver, Scott C.. Ut Medical Branch At Galveston;Fil: Reis, Mitermayer G.. Universidade Federal da Bahia; Brasil. Fundación Oswaldo Cruz; BrasilFil: Kitron, Uriel D.. Fundación Oswaldo Cruz; Brasil. University of Emory; Estados UnidosFil: Ribeiro, Guilherme S.. Universidade Federal da Bahia; Brasil. Fundación Oswaldo Cruz; BrasilBioMed Central2018-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/81470Kikuti, Mariana; Tauro, Laura Beatriz; Moreira, Patrícia S. S.; Campos, Gúbio S.; Paploski, Igor A. D.; et al.; Diagnostic performance of commercial IgM and IgG enzyme-linked immunoassays (ELISAs) for diagnosis of Zika virus infection; BioMed Central; Virology Journal; 15; 1; 7-2018; 1-71743-422XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://virologyj.biomedcentral.com/articles/10.1186/s12985-018-1015-6info:eu-repo/semantics/altIdentifier/doi/10.1186/s12985-018-1015-6info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:02:14Zoai:ri.conicet.gov.ar:11336/81470instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:02:14.53CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Diagnostic performance of commercial IgM and IgG enzyme-linked immunoassays (ELISAs) for diagnosis of Zika virus infection
title Diagnostic performance of commercial IgM and IgG enzyme-linked immunoassays (ELISAs) for diagnosis of Zika virus infection
spellingShingle Diagnostic performance of commercial IgM and IgG enzyme-linked immunoassays (ELISAs) for diagnosis of Zika virus infection
Kikuti, Mariana
ENZYME-LINKED IMMUNOSORBENT ASSAY
IMMUNOGLOBULIN G
IMMUNOGLOBULIN M
SENSITIVITY AND SPECIFICITY
ZIKA VIRUS
title_short Diagnostic performance of commercial IgM and IgG enzyme-linked immunoassays (ELISAs) for diagnosis of Zika virus infection
title_full Diagnostic performance of commercial IgM and IgG enzyme-linked immunoassays (ELISAs) for diagnosis of Zika virus infection
title_fullStr Diagnostic performance of commercial IgM and IgG enzyme-linked immunoassays (ELISAs) for diagnosis of Zika virus infection
title_full_unstemmed Diagnostic performance of commercial IgM and IgG enzyme-linked immunoassays (ELISAs) for diagnosis of Zika virus infection
title_sort Diagnostic performance of commercial IgM and IgG enzyme-linked immunoassays (ELISAs) for diagnosis of Zika virus infection
dc.creator.none.fl_str_mv Kikuti, Mariana
Tauro, Laura Beatriz
Moreira, Patrícia S. S.
Campos, Gúbio S.
Paploski, Igor A. D.
Weaver, Scott C.
Reis, Mitermayer G.
Kitron, Uriel D.
Ribeiro, Guilherme S.
author Kikuti, Mariana
author_facet Kikuti, Mariana
Tauro, Laura Beatriz
Moreira, Patrícia S. S.
Campos, Gúbio S.
Paploski, Igor A. D.
Weaver, Scott C.
Reis, Mitermayer G.
Kitron, Uriel D.
Ribeiro, Guilherme S.
author_role author
author2 Tauro, Laura Beatriz
Moreira, Patrícia S. S.
Campos, Gúbio S.
Paploski, Igor A. D.
Weaver, Scott C.
Reis, Mitermayer G.
Kitron, Uriel D.
Ribeiro, Guilherme S.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ENZYME-LINKED IMMUNOSORBENT ASSAY
IMMUNOGLOBULIN G
IMMUNOGLOBULIN M
SENSITIVITY AND SPECIFICITY
ZIKA VIRUS
topic ENZYME-LINKED IMMUNOSORBENT ASSAY
IMMUNOGLOBULIN G
IMMUNOGLOBULIN M
SENSITIVITY AND SPECIFICITY
ZIKA VIRUS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: Serologic detection of Zika virus (ZIKV) infections is challenging because of antigenic similarities among flaviviruses. Objective: To evaluate the sensitivity and specificity of commercial ZIKV IgM and IgG enzyme-linked immunoassay (ELISA) kits. Methods: We used sera from febrile patients with RT-PCR-confirmed ZIKV infection to determine sensitivity and sera from RT-PCR-confirmed dengue cases and blood donors, both of which were collected before ZIKV epidemics in Brazil (2009-2011 and 2013, respectively) to determine specificity. Results: The ZIKV IgM-ELISA positivity among RT-PCR ZIKV confirmed cases was 0.0% (0/14) and 12.5% (1/8) for acute- and convalescent-phase sera, respectively, while its specificity was 100.0% (58/58) and 98.3% (58/59) for acute- and convalescent-phase sera of dengue patients, and 100.0% (23/23) for blood donors. The ZIKV IgG-ELISA sensitivity was 100.0% (6/6) on convalescent-phase sera from RT-PCR confirmed ZIKV patients, while its specificity was 27.3% (15/55) on convalescent-phase sera from dengue patients and 45.0% (9/20) on blood donors' sera. The ZIKV IgG-ELISA specificity among dengue confirmed cases was much greater among patients with primary dengue (92.3%; 12/13), compared to secondary dengue (7.1%; 3/42). Conclusions: In a setting of endemic dengue transmission, the ZIKV IgM-ELISA had high specificity, but poor sensitivity. In contrast, the ZIKV IgG-ELISA showed low specificity, particularly for patients previously exposed to dengue infections. This suggests that this ZIKV IgM-ELISA is not useful in confirming a diagnosis of ZIKV infection in suspected patients, whereas the IgG-ELISA is more suitable for ZIKV diagnosis among travelers, who reside in areas free of flavivirus transmission, rather than for serosurveys in dengue-endemic areas.
Fil: Kikuti, Mariana. Fundación Oswaldo Cruz; Brasil. Universidade Federal da Bahia; Brasil
Fil: Tauro, Laura Beatriz. Fundación Oswaldo Cruz; Brasil. Ministerio de Salud. Instituto Nacional de Medicina Tropical; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú; Argentina
Fil: Moreira, Patrícia S. S.. Fundación Oswaldo Cruz; Brasil
Fil: Campos, Gúbio S.. Universidade Federal da Bahia; Brasil
Fil: Paploski, Igor A. D.. Fundación Oswaldo Cruz; Brasil. Universidade Federal da Bahia; Brasil
Fil: Weaver, Scott C.. Ut Medical Branch At Galveston;
Fil: Reis, Mitermayer G.. Universidade Federal da Bahia; Brasil. Fundación Oswaldo Cruz; Brasil
Fil: Kitron, Uriel D.. Fundación Oswaldo Cruz; Brasil. University of Emory; Estados Unidos
Fil: Ribeiro, Guilherme S.. Universidade Federal da Bahia; Brasil. Fundación Oswaldo Cruz; Brasil
description Background: Serologic detection of Zika virus (ZIKV) infections is challenging because of antigenic similarities among flaviviruses. Objective: To evaluate the sensitivity and specificity of commercial ZIKV IgM and IgG enzyme-linked immunoassay (ELISA) kits. Methods: We used sera from febrile patients with RT-PCR-confirmed ZIKV infection to determine sensitivity and sera from RT-PCR-confirmed dengue cases and blood donors, both of which were collected before ZIKV epidemics in Brazil (2009-2011 and 2013, respectively) to determine specificity. Results: The ZIKV IgM-ELISA positivity among RT-PCR ZIKV confirmed cases was 0.0% (0/14) and 12.5% (1/8) for acute- and convalescent-phase sera, respectively, while its specificity was 100.0% (58/58) and 98.3% (58/59) for acute- and convalescent-phase sera of dengue patients, and 100.0% (23/23) for blood donors. The ZIKV IgG-ELISA sensitivity was 100.0% (6/6) on convalescent-phase sera from RT-PCR confirmed ZIKV patients, while its specificity was 27.3% (15/55) on convalescent-phase sera from dengue patients and 45.0% (9/20) on blood donors' sera. The ZIKV IgG-ELISA specificity among dengue confirmed cases was much greater among patients with primary dengue (92.3%; 12/13), compared to secondary dengue (7.1%; 3/42). Conclusions: In a setting of endemic dengue transmission, the ZIKV IgM-ELISA had high specificity, but poor sensitivity. In contrast, the ZIKV IgG-ELISA showed low specificity, particularly for patients previously exposed to dengue infections. This suggests that this ZIKV IgM-ELISA is not useful in confirming a diagnosis of ZIKV infection in suspected patients, whereas the IgG-ELISA is more suitable for ZIKV diagnosis among travelers, who reside in areas free of flavivirus transmission, rather than for serosurveys in dengue-endemic areas.
publishDate 2018
dc.date.none.fl_str_mv 2018-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/81470
Kikuti, Mariana; Tauro, Laura Beatriz; Moreira, Patrícia S. S.; Campos, Gúbio S.; Paploski, Igor A. D.; et al.; Diagnostic performance of commercial IgM and IgG enzyme-linked immunoassays (ELISAs) for diagnosis of Zika virus infection; BioMed Central; Virology Journal; 15; 1; 7-2018; 1-7
1743-422X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/81470
identifier_str_mv Kikuti, Mariana; Tauro, Laura Beatriz; Moreira, Patrícia S. S.; Campos, Gúbio S.; Paploski, Igor A. D.; et al.; Diagnostic performance of commercial IgM and IgG enzyme-linked immunoassays (ELISAs) for diagnosis of Zika virus infection; BioMed Central; Virology Journal; 15; 1; 7-2018; 1-7
1743-422X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://virologyj.biomedcentral.com/articles/10.1186/s12985-018-1015-6
info:eu-repo/semantics/altIdentifier/doi/10.1186/s12985-018-1015-6
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.885934

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