Iron and zinc biofortification in potato through the introduction of NICOTIANAMINE SYNTHASE and FERRITIN genes

Autores
Cortelezzi, Juan Ignacio; Zubillaga, Martina; Scardino, Victoria Raquel; Fumagalli, Marina; Muñiz García, María Noelia; Capiati, Daniela Andrea
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Micronutrient deficiencies, such as those involving iron, zinc, and vitamin A, represent a critical global public health challenge. Crop biofortification offers a costeffective solution by enhancing the micronutrient content of staple foods, particularly in regions with limited access to diverse diets. In this study, we developed a high-iron and high-zinc Spunta potato variety through the co-expression of the Arabidopsis thaliana NICOTIANAMINE SYNTHASE 1 (AtNAS1) gene under a constitutive promoter and the Phaseolus vulgaris FERRITIN (PvFERRITIN) gene under a tuber-specific promoter. Of the five co-expressing events evaluated, we identified FN4 as the optimal line, achieving significant levels of iron and zinc biofortification without compromising yield. This line exhibited an enhanced capacity to accumulate iron in tubers under conditions of increased soil iron availability, maintaining yield even when vegetative growth was reduced due to elevated soil iron levels. The FN4 variety showed a 2.1- and 1.8-fold increase in iron and zinc content, respectively, in the tubers. A typical portion of this variety could provide 33.3% of the recommended dietary allowance (RDA) for iron and 19.8% for zinc in women of reproductive age. This biofortified potato has the potential to reduce iron deficiency anemia and zinc deficiency, enhancing the health of populations in both rural and urban areas affected by poverty.
Fil: Cortelezzi, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Zubillaga, Martina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Scardino, Victoria Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Fumagalli, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Muñiz García, María Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Capiati, Daniela Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Materia
POTATO
SPUNTA
BIOFORTIFICATION
IRON
ZINC
FERRITIN
NICOTIANAMINA SYNTHASE
NAS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/279972
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Iron and zinc biofortification in potato through the introduction of NICOTIANAMINE SYNTHASE and FERRITIN genesCortelezzi, Juan IgnacioZubillaga, MartinaScardino, Victoria RaquelFumagalli, MarinaMuñiz García, María NoeliaCapiati, Daniela AndreaPOTATOSPUNTABIOFORTIFICATIONIRONZINCFERRITINNICOTIANAMINA SYNTHASENAShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Micronutrient deficiencies, such as those involving iron, zinc, and vitamin A, represent a critical global public health challenge. Crop biofortification offers a costeffective solution by enhancing the micronutrient content of staple foods, particularly in regions with limited access to diverse diets. In this study, we developed a high-iron and high-zinc Spunta potato variety through the co-expression of the Arabidopsis thaliana NICOTIANAMINE SYNTHASE 1 (AtNAS1) gene under a constitutive promoter and the Phaseolus vulgaris FERRITIN (PvFERRITIN) gene under a tuber-specific promoter. Of the five co-expressing events evaluated, we identified FN4 as the optimal line, achieving significant levels of iron and zinc biofortification without compromising yield. This line exhibited an enhanced capacity to accumulate iron in tubers under conditions of increased soil iron availability, maintaining yield even when vegetative growth was reduced due to elevated soil iron levels. The FN4 variety showed a 2.1- and 1.8-fold increase in iron and zinc content, respectively, in the tubers. A typical portion of this variety could provide 33.3% of the recommended dietary allowance (RDA) for iron and 19.8% for zinc in women of reproductive age. This biofortified potato has the potential to reduce iron deficiency anemia and zinc deficiency, enhancing the health of populations in both rural and urban areas affected by poverty.Fil: Cortelezzi, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; ArgentinaFil: Zubillaga, Martina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; ArgentinaFil: Scardino, Victoria Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Fumagalli, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Muñiz García, María Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Capiati, Daniela Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; ArgentinaSpringer2025-10-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/279972Cortelezzi, Juan Ignacio; Zubillaga, Martina; Scardino, Victoria Raquel; Fumagalli, Marina; Muñiz García, María Noelia; et al.; Iron and zinc biofortification in potato through the introduction of NICOTIANAMINE SYNTHASE and FERRITIN genes; Springer; Molecular Breeding; 45; 30-10-2025; 1-211572-97881380-3743CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s11032-025-01619-4info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1007/s11032-025-01619-4info:eu-repo/semantics/altIdentifier/arxiv/https://www.biorxiv.org/lookup/doi/10.1101/2025.03.01.640813info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-06T13:25:54Zoai:ri.conicet.gov.ar:11336/279972instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-06 13:25:55.125CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Iron and zinc biofortification in potato through the introduction of NICOTIANAMINE SYNTHASE and FERRITIN genes
title Iron and zinc biofortification in potato through the introduction of NICOTIANAMINE SYNTHASE and FERRITIN genes
spellingShingle Iron and zinc biofortification in potato through the introduction of NICOTIANAMINE SYNTHASE and FERRITIN genes
Cortelezzi, Juan Ignacio
POTATO
SPUNTA
BIOFORTIFICATION
IRON
ZINC
FERRITIN
NICOTIANAMINA SYNTHASE
NAS
title_short Iron and zinc biofortification in potato through the introduction of NICOTIANAMINE SYNTHASE and FERRITIN genes
title_full Iron and zinc biofortification in potato through the introduction of NICOTIANAMINE SYNTHASE and FERRITIN genes
title_fullStr Iron and zinc biofortification in potato through the introduction of NICOTIANAMINE SYNTHASE and FERRITIN genes
title_full_unstemmed Iron and zinc biofortification in potato through the introduction of NICOTIANAMINE SYNTHASE and FERRITIN genes
title_sort Iron and zinc biofortification in potato through the introduction of NICOTIANAMINE SYNTHASE and FERRITIN genes
dc.creator.none.fl_str_mv Cortelezzi, Juan Ignacio
Zubillaga, Martina
Scardino, Victoria Raquel
Fumagalli, Marina
Muñiz García, María Noelia
Capiati, Daniela Andrea
author Cortelezzi, Juan Ignacio
author_facet Cortelezzi, Juan Ignacio
Zubillaga, Martina
Scardino, Victoria Raquel
Fumagalli, Marina
Muñiz García, María Noelia
Capiati, Daniela Andrea
author_role author
author2 Zubillaga, Martina
Scardino, Victoria Raquel
Fumagalli, Marina
Muñiz García, María Noelia
Capiati, Daniela Andrea
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv POTATO
SPUNTA
BIOFORTIFICATION
IRON
ZINC
FERRITIN
NICOTIANAMINA SYNTHASE
NAS
topic POTATO
SPUNTA
BIOFORTIFICATION
IRON
ZINC
FERRITIN
NICOTIANAMINA SYNTHASE
NAS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Micronutrient deficiencies, such as those involving iron, zinc, and vitamin A, represent a critical global public health challenge. Crop biofortification offers a costeffective solution by enhancing the micronutrient content of staple foods, particularly in regions with limited access to diverse diets. In this study, we developed a high-iron and high-zinc Spunta potato variety through the co-expression of the Arabidopsis thaliana NICOTIANAMINE SYNTHASE 1 (AtNAS1) gene under a constitutive promoter and the Phaseolus vulgaris FERRITIN (PvFERRITIN) gene under a tuber-specific promoter. Of the five co-expressing events evaluated, we identified FN4 as the optimal line, achieving significant levels of iron and zinc biofortification without compromising yield. This line exhibited an enhanced capacity to accumulate iron in tubers under conditions of increased soil iron availability, maintaining yield even when vegetative growth was reduced due to elevated soil iron levels. The FN4 variety showed a 2.1- and 1.8-fold increase in iron and zinc content, respectively, in the tubers. A typical portion of this variety could provide 33.3% of the recommended dietary allowance (RDA) for iron and 19.8% for zinc in women of reproductive age. This biofortified potato has the potential to reduce iron deficiency anemia and zinc deficiency, enhancing the health of populations in both rural and urban areas affected by poverty.
Fil: Cortelezzi, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Zubillaga, Martina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Scardino, Victoria Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Fumagalli, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Muñiz García, María Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Capiati, Daniela Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
description Micronutrient deficiencies, such as those involving iron, zinc, and vitamin A, represent a critical global public health challenge. Crop biofortification offers a costeffective solution by enhancing the micronutrient content of staple foods, particularly in regions with limited access to diverse diets. In this study, we developed a high-iron and high-zinc Spunta potato variety through the co-expression of the Arabidopsis thaliana NICOTIANAMINE SYNTHASE 1 (AtNAS1) gene under a constitutive promoter and the Phaseolus vulgaris FERRITIN (PvFERRITIN) gene under a tuber-specific promoter. Of the five co-expressing events evaluated, we identified FN4 as the optimal line, achieving significant levels of iron and zinc biofortification without compromising yield. This line exhibited an enhanced capacity to accumulate iron in tubers under conditions of increased soil iron availability, maintaining yield even when vegetative growth was reduced due to elevated soil iron levels. The FN4 variety showed a 2.1- and 1.8-fold increase in iron and zinc content, respectively, in the tubers. A typical portion of this variety could provide 33.3% of the recommended dietary allowance (RDA) for iron and 19.8% for zinc in women of reproductive age. This biofortified potato has the potential to reduce iron deficiency anemia and zinc deficiency, enhancing the health of populations in both rural and urban areas affected by poverty.
publishDate 2025
dc.date.none.fl_str_mv 2025-10-30
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/279972
Cortelezzi, Juan Ignacio; Zubillaga, Martina; Scardino, Victoria Raquel; Fumagalli, Marina; Muñiz García, María Noelia; et al.; Iron and zinc biofortification in potato through the introduction of NICOTIANAMINE SYNTHASE and FERRITIN genes; Springer; Molecular Breeding; 45; 30-10-2025; 1-21
1572-9788
1380-3743
CONICET Digital
CONICET
url http://hdl.handle.net/11336/279972
identifier_str_mv Cortelezzi, Juan Ignacio; Zubillaga, Martina; Scardino, Victoria Raquel; Fumagalli, Marina; Muñiz García, María Noelia; et al.; Iron and zinc biofortification in potato through the introduction of NICOTIANAMINE SYNTHASE and FERRITIN genes; Springer; Molecular Breeding; 45; 30-10-2025; 1-21
1572-9788
1380-3743
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s11032-025-01619-4
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1007/s11032-025-01619-4
info:eu-repo/semantics/altIdentifier/arxiv/https://www.biorxiv.org/lookup/doi/10.1101/2025.03.01.640813
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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