Prospective uses of recombinant Lactococcus lactis expressing both listeriolysin O and mutated internalin A from Listeria monocytogenes as a tool for DNA vaccination

Autores
de Azevedo, M. S. P.; Santos Rocha, C.; Pereira, V. B.; de Oliveira Junior, A. .; de Sousa, C. S.; Azevedo, V.; Leblanc, Jean Guy Joseph; Chatel, J. M.; Miyoshi, A.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this study, Lactococcus lactis was engineered to express mutated internalin A on its surface and to secrete large amounts of listeriolysin O (LLO) in order to improve its potential as a vehicle for DNA vaccination. Western blotting experiments demonstrated that the bacterium expressed LLO in both the cytoplasmic and extracellular compartments, with higher quantities found in the culture supernatants. A hemolytic assay showed that the recombinant strain secreted 250 ng active LLO/mg total protein. This mInlA/LLO-producing strain of L. lactis may be used as an alternative tool in DNA vaccination against a number of infectious diseases or in cancer therapy.
Fil: de Azevedo, M. S. P.. Universidade Federal de Minas Gerais; Brasil
Fil: Santos Rocha, C.. Universidade Federal de Minas Gerais; Brasil
Fil: Pereira, V. B.. Universidade Federal de Minas Gerais; Brasil
Fil: de Oliveira Junior, A. .. Universidade Federal de Minas Gerais; Brasil
Fil: de Sousa, C. S.. Universidade Federal de Minas Gerais; Brasil
Fil: Azevedo, V.. Universidade Federal de Minas Gerais; Brasil
Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Chatel, J. M.. Institut National de la Recherche Agronomique; Francia
Fil: Miyoshi, A.. Universidade Federal de Minas Gerais; Brasil
Materia
LACTIC ACID BACTERIA
LACTOCOCCUS LACTIS
LISTERIA MONOCYTOGENES
LISTERIOLYSIN O
MUTATED INTERNALIN A
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/78738
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/78738
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Prospective uses of recombinant Lactococcus lactis expressing both listeriolysin O and mutated internalin A from Listeria monocytogenes as a tool for DNA vaccinationde Azevedo, M. S. P.Santos Rocha, C.Pereira, V. B.de Oliveira Junior, A. .de Sousa, C. S.Azevedo, V.Leblanc, Jean Guy JosephChatel, J. M.Miyoshi, A.LACTIC ACID BACTERIALACTOCOCCUS LACTISLISTERIA MONOCYTOGENESLISTERIOLYSIN OMUTATED INTERNALIN Ahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In this study, Lactococcus lactis was engineered to express mutated internalin A on its surface and to secrete large amounts of listeriolysin O (LLO) in order to improve its potential as a vehicle for DNA vaccination. Western blotting experiments demonstrated that the bacterium expressed LLO in both the cytoplasmic and extracellular compartments, with higher quantities found in the culture supernatants. A hemolytic assay showed that the recombinant strain secreted 250 ng active LLO/mg total protein. This mInlA/LLO-producing strain of L. lactis may be used as an alternative tool in DNA vaccination against a number of infectious diseases or in cancer therapy.Fil: de Azevedo, M. S. P.. Universidade Federal de Minas Gerais; BrasilFil: Santos Rocha, C.. Universidade Federal de Minas Gerais; BrasilFil: Pereira, V. B.. Universidade Federal de Minas Gerais; BrasilFil: de Oliveira Junior, A. .. Universidade Federal de Minas Gerais; BrasilFil: de Sousa, C. S.. Universidade Federal de Minas Gerais; BrasilFil: Azevedo, V.. Universidade Federal de Minas Gerais; BrasilFil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Chatel, J. M.. Institut National de la Recherche Agronomique; FranciaFil: Miyoshi, A.. Universidade Federal de Minas Gerais; BrasilFundacao de Pesquisas Científicas de Riberao Preto2015-12-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/78738de Azevedo, M. S. P.; Santos Rocha, C.; Pereira, V. B.; de Oliveira Junior, A. .; de Sousa, C. S.; et al.; Prospective uses of recombinant Lactococcus lactis expressing both listeriolysin O and mutated internalin A from Listeria monocytogenes as a tool for DNA vaccination; Fundacao de Pesquisas Científicas de Riberao Preto; Genetics and Molecular Research; 14; 4; 28-12-2015; 18485-184931676-5680CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4238/2015.December.23.36info:eu-repo/semantics/altIdentifier/url/https://www.geneticsmr.com/articles/5859info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:59:45Zoai:ri.conicet.gov.ar:11336/78738instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:59:46.117CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Prospective uses of recombinant Lactococcus lactis expressing both listeriolysin O and mutated internalin A from Listeria monocytogenes as a tool for DNA vaccination
title Prospective uses of recombinant Lactococcus lactis expressing both listeriolysin O and mutated internalin A from Listeria monocytogenes as a tool for DNA vaccination
spellingShingle Prospective uses of recombinant Lactococcus lactis expressing both listeriolysin O and mutated internalin A from Listeria monocytogenes as a tool for DNA vaccination
de Azevedo, M. S. P.
LACTIC ACID BACTERIA
LACTOCOCCUS LACTIS
LISTERIA MONOCYTOGENES
LISTERIOLYSIN O
MUTATED INTERNALIN A
title_short Prospective uses of recombinant Lactococcus lactis expressing both listeriolysin O and mutated internalin A from Listeria monocytogenes as a tool for DNA vaccination
title_full Prospective uses of recombinant Lactococcus lactis expressing both listeriolysin O and mutated internalin A from Listeria monocytogenes as a tool for DNA vaccination
title_fullStr Prospective uses of recombinant Lactococcus lactis expressing both listeriolysin O and mutated internalin A from Listeria monocytogenes as a tool for DNA vaccination
title_full_unstemmed Prospective uses of recombinant Lactococcus lactis expressing both listeriolysin O and mutated internalin A from Listeria monocytogenes as a tool for DNA vaccination
title_sort Prospective uses of recombinant Lactococcus lactis expressing both listeriolysin O and mutated internalin A from Listeria monocytogenes as a tool for DNA vaccination
dc.creator.none.fl_str_mv de Azevedo, M. S. P.
Santos Rocha, C.
Pereira, V. B.
de Oliveira Junior, A. .
de Sousa, C. S.
Azevedo, V.
Leblanc, Jean Guy Joseph
Chatel, J. M.
Miyoshi, A.
author de Azevedo, M. S. P.
author_facet de Azevedo, M. S. P.
Santos Rocha, C.
Pereira, V. B.
de Oliveira Junior, A. .
de Sousa, C. S.
Azevedo, V.
Leblanc, Jean Guy Joseph
Chatel, J. M.
Miyoshi, A.
author_role author
author2 Santos Rocha, C.
Pereira, V. B.
de Oliveira Junior, A. .
de Sousa, C. S.
Azevedo, V.
Leblanc, Jean Guy Joseph
Chatel, J. M.
Miyoshi, A.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv LACTIC ACID BACTERIA
LACTOCOCCUS LACTIS
LISTERIA MONOCYTOGENES
LISTERIOLYSIN O
MUTATED INTERNALIN A
topic LACTIC ACID BACTERIA
LACTOCOCCUS LACTIS
LISTERIA MONOCYTOGENES
LISTERIOLYSIN O
MUTATED INTERNALIN A
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In this study, Lactococcus lactis was engineered to express mutated internalin A on its surface and to secrete large amounts of listeriolysin O (LLO) in order to improve its potential as a vehicle for DNA vaccination. Western blotting experiments demonstrated that the bacterium expressed LLO in both the cytoplasmic and extracellular compartments, with higher quantities found in the culture supernatants. A hemolytic assay showed that the recombinant strain secreted 250 ng active LLO/mg total protein. This mInlA/LLO-producing strain of L. lactis may be used as an alternative tool in DNA vaccination against a number of infectious diseases or in cancer therapy.
Fil: de Azevedo, M. S. P.. Universidade Federal de Minas Gerais; Brasil
Fil: Santos Rocha, C.. Universidade Federal de Minas Gerais; Brasil
Fil: Pereira, V. B.. Universidade Federal de Minas Gerais; Brasil
Fil: de Oliveira Junior, A. .. Universidade Federal de Minas Gerais; Brasil
Fil: de Sousa, C. S.. Universidade Federal de Minas Gerais; Brasil
Fil: Azevedo, V.. Universidade Federal de Minas Gerais; Brasil
Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Chatel, J. M.. Institut National de la Recherche Agronomique; Francia
Fil: Miyoshi, A.. Universidade Federal de Minas Gerais; Brasil
description In this study, Lactococcus lactis was engineered to express mutated internalin A on its surface and to secrete large amounts of listeriolysin O (LLO) in order to improve its potential as a vehicle for DNA vaccination. Western blotting experiments demonstrated that the bacterium expressed LLO in both the cytoplasmic and extracellular compartments, with higher quantities found in the culture supernatants. A hemolytic assay showed that the recombinant strain secreted 250 ng active LLO/mg total protein. This mInlA/LLO-producing strain of L. lactis may be used as an alternative tool in DNA vaccination against a number of infectious diseases or in cancer therapy.
publishDate 2015
dc.date.none.fl_str_mv 2015-12-28
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/78738
de Azevedo, M. S. P.; Santos Rocha, C.; Pereira, V. B.; de Oliveira Junior, A. .; de Sousa, C. S.; et al.; Prospective uses of recombinant Lactococcus lactis expressing both listeriolysin O and mutated internalin A from Listeria monocytogenes as a tool for DNA vaccination; Fundacao de Pesquisas Científicas de Riberao Preto; Genetics and Molecular Research; 14; 4; 28-12-2015; 18485-18493
1676-5680
CONICET Digital
CONICET
url http://hdl.handle.net/11336/78738
identifier_str_mv de Azevedo, M. S. P.; Santos Rocha, C.; Pereira, V. B.; de Oliveira Junior, A. .; de Sousa, C. S.; et al.; Prospective uses of recombinant Lactococcus lactis expressing both listeriolysin O and mutated internalin A from Listeria monocytogenes as a tool for DNA vaccination; Fundacao de Pesquisas Científicas de Riberao Preto; Genetics and Molecular Research; 14; 4; 28-12-2015; 18485-18493
1676-5680
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4238/2015.December.23.36
info:eu-repo/semantics/altIdentifier/url/https://www.geneticsmr.com/articles/5859
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Fundacao de Pesquisas Científicas de Riberao Preto
publisher.none.fl_str_mv Fundacao de Pesquisas Científicas de Riberao Preto
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269599629836288
score 13.13397

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