Recombinant invasive Lactococcus lactis can transfer DNA vaccines either directly to dendritic cells or across an epithelial cell monolayer

Autores
de Azevedo, Marcela; Meijerink, Marjolein; Taverne, Nico; Bastos Pereira, Vanessa; Leblanc, Jean Guy Joseph; Azevedo, Vasco; Miyoshi, Anderson; Langella, Philippe; Wells, Jerry M.; Chatel, Jean Marc
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lactococcus lactis (L. lactis), a generally regarded as safe (GRAS) bacterium has recently been investigated as a mucosal delivery vehicle for DNA vaccines. Because of its GRAS status, L. lactis represents an attractive alternative to attenuated pathogens. Previous studies showed that eukaryotic expression plasmids could be delivered into intestinal epithelial cells (IECs) by L. lactis, or recombinant invasive strains of L. lactis, leading to heterologous protein expression. Although expression of antigens in IECs might lead to vaccine responses, it would be of interest to know whether uptake of L. lactis DNA vaccines by dendritic cells (DCs) could lead to antigen expression as they are unique in their ability to induce antigen-specific T cell responses. To test this, we incubated mouse bone marrow-derived DCs (BMDCs) with invasive L. lactis strains expressing either Staphylococcus aureus Fibronectin Binding Protein A (LL-FnBPA+), or Listeria monocytogenes mutated Internalin A (LL-mInlA+), both strains carrying a plasmid DNA vaccine (pValac) encoding for the cow milk allergen β-lactoglobulin (BLG). We demonstrated that they can transfect BMDCs, inducing the secretion of the pro-inflammatory cytokine IL-12. We also measured the capacity of strains to invade a polarized monolayer of IECs, mimicking the situation encountered in the gastrointestinal tract. Gentamycin survival assay in these cells showed that LL-mInlA+ is 100 times more invasive than L. lactis. The cross-talk between differentiated IECs, BMDCs and bacteria was also evaluated using an in vitro transwell co-culture model. Co-incubation of strains in this model showed that DCs incubated with LL-mInlA+ containing pValac:BLG could express significant levels of BLG. These results suggest that DCs could sample bacteria containing the DNA vaccine across the epithelial barrier and express the antigen.
Fil: de Azevedo, Marcela. Universidade Federal de Minas Gerais; Brasil
Fil: Meijerink, Marjolein. Wageningen University. Host Microbe Interactomics; Países Bajos
Fil: Taverne, Nico. Wageningen University. Host Microbe Interactomics; Países Bajos
Fil: Bastos Pereira, Vanessa. Universidade Federal de Minas Gerais; Brasil
Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
Fil: Azevedo, Vasco. Universidade Federal de Minas Gerais; Brasil
Fil: Miyoshi, Anderson. Universidade Federal de Minas Gerais; Brasil
Fil: Langella, Philippe. Institut National de la Recherche Agronomique; Francia. AgroParisTech; Francia
Fil: Wells, Jerry M.. Wageningen University. Host Microbe Interactomics; Países Bajos
Fil: Chatel, Jean Marc. Institut National de la Recherche Agronomique; Francia. AgroParisTech; Francia
Materia
DENDRITIC CELLS
DNA DELIVERY
INTERNALIZATION
LACTOCOCCUS LACTIS
LISTERIA MONOCYTOGENES
MUTATED INTERNALIN A
Β-LACTOGLOBULIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/2910
Acceder
id CONICETDig_13f727e00166c404d9dcbe0db1a3185d
oai_identifier_str oai:ri.conicet.gov.ar:11336/2910
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Recombinant invasive Lactococcus lactis can transfer DNA vaccines either directly to dendritic cells or across an epithelial cell monolayerde Azevedo, MarcelaMeijerink, MarjoleinTaverne, NicoBastos Pereira, VanessaLeblanc, Jean Guy JosephAzevedo, VascoMiyoshi, AndersonLangella, PhilippeWells, Jerry M.Chatel, Jean MarcDENDRITIC CELLSDNA DELIVERYINTERNALIZATIONLACTOCOCCUS LACTISLISTERIA MONOCYTOGENESMUTATED INTERNALIN AΒ-LACTOGLOBULINhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Lactococcus lactis (L. lactis), a generally regarded as safe (GRAS) bacterium has recently been investigated as a mucosal delivery vehicle for DNA vaccines. Because of its GRAS status, L. lactis represents an attractive alternative to attenuated pathogens. Previous studies showed that eukaryotic expression plasmids could be delivered into intestinal epithelial cells (IECs) by L. lactis, or recombinant invasive strains of L. lactis, leading to heterologous protein expression. Although expression of antigens in IECs might lead to vaccine responses, it would be of interest to know whether uptake of L. lactis DNA vaccines by dendritic cells (DCs) could lead to antigen expression as they are unique in their ability to induce antigen-specific T cell responses. To test this, we incubated mouse bone marrow-derived DCs (BMDCs) with invasive L. lactis strains expressing either Staphylococcus aureus Fibronectin Binding Protein A (LL-FnBPA+), or Listeria monocytogenes mutated Internalin A (LL-mInlA+), both strains carrying a plasmid DNA vaccine (pValac) encoding for the cow milk allergen β-lactoglobulin (BLG). We demonstrated that they can transfect BMDCs, inducing the secretion of the pro-inflammatory cytokine IL-12. We also measured the capacity of strains to invade a polarized monolayer of IECs, mimicking the situation encountered in the gastrointestinal tract. Gentamycin survival assay in these cells showed that LL-mInlA+ is 100 times more invasive than L. lactis. The cross-talk between differentiated IECs, BMDCs and bacteria was also evaluated using an in vitro transwell co-culture model. Co-incubation of strains in this model showed that DCs incubated with LL-mInlA+ containing pValac:BLG could express significant levels of BLG. These results suggest that DCs could sample bacteria containing the DNA vaccine across the epithelial barrier and express the antigen.Fil: de Azevedo, Marcela. Universidade Federal de Minas Gerais; BrasilFil: Meijerink, Marjolein. Wageningen University. Host Microbe Interactomics; Países BajosFil: Taverne, Nico. Wageningen University. Host Microbe Interactomics; Países BajosFil: Bastos Pereira, Vanessa. Universidade Federal de Minas Gerais; BrasilFil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); ArgentinaFil: Azevedo, Vasco. Universidade Federal de Minas Gerais; BrasilFil: Miyoshi, Anderson. Universidade Federal de Minas Gerais; BrasilFil: Langella, Philippe. Institut National de la Recherche Agronomique; Francia. AgroParisTech; FranciaFil: Wells, Jerry M.. Wageningen University. Host Microbe Interactomics; Países BajosFil: Chatel, Jean Marc. Institut National de la Recherche Agronomique; Francia. AgroParisTech; FranciaElsevier2015-09-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2910de Azevedo, Marcela ; Meijerink, Marjolein; Taverne, Nico ; Bastos Pereira, Vanessa ; Leblanc, Jean Guy Joseph; et al.; Recombinant invasive Lactococcus lactis can transfer DNA vaccines either directly to dendritic cells or across an epithelial cell monolayer; Elsevier; Vaccine; 33; 38; 11-9-2015; 4807-48120264-410X1873-2518enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/journal/0264410Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.vaccine.2015.07.077info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:14:03Zoai:ri.conicet.gov.ar:11336/2910instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:14:03.446CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Recombinant invasive Lactococcus lactis can transfer DNA vaccines either directly to dendritic cells or across an epithelial cell monolayer
title Recombinant invasive Lactococcus lactis can transfer DNA vaccines either directly to dendritic cells or across an epithelial cell monolayer
spellingShingle Recombinant invasive Lactococcus lactis can transfer DNA vaccines either directly to dendritic cells or across an epithelial cell monolayer
de Azevedo, Marcela
DENDRITIC CELLS
DNA DELIVERY
INTERNALIZATION
LACTOCOCCUS LACTIS
LISTERIA MONOCYTOGENES
MUTATED INTERNALIN A
Β-LACTOGLOBULIN
title_short Recombinant invasive Lactococcus lactis can transfer DNA vaccines either directly to dendritic cells or across an epithelial cell monolayer
title_full Recombinant invasive Lactococcus lactis can transfer DNA vaccines either directly to dendritic cells or across an epithelial cell monolayer
title_fullStr Recombinant invasive Lactococcus lactis can transfer DNA vaccines either directly to dendritic cells or across an epithelial cell monolayer
title_full_unstemmed Recombinant invasive Lactococcus lactis can transfer DNA vaccines either directly to dendritic cells or across an epithelial cell monolayer
title_sort Recombinant invasive Lactococcus lactis can transfer DNA vaccines either directly to dendritic cells or across an epithelial cell monolayer
dc.creator.none.fl_str_mv de Azevedo, Marcela
Meijerink, Marjolein
Taverne, Nico
Bastos Pereira, Vanessa
Leblanc, Jean Guy Joseph
Azevedo, Vasco
Miyoshi, Anderson
Langella, Philippe
Wells, Jerry M.
Chatel, Jean Marc
author de Azevedo, Marcela
author_facet de Azevedo, Marcela
Meijerink, Marjolein
Taverne, Nico
Bastos Pereira, Vanessa
Leblanc, Jean Guy Joseph
Azevedo, Vasco
Miyoshi, Anderson
Langella, Philippe
Wells, Jerry M.
Chatel, Jean Marc
author_role author
author2 Meijerink, Marjolein
Taverne, Nico
Bastos Pereira, Vanessa
Leblanc, Jean Guy Joseph
Azevedo, Vasco
Miyoshi, Anderson
Langella, Philippe
Wells, Jerry M.
Chatel, Jean Marc
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv DENDRITIC CELLS
DNA DELIVERY
INTERNALIZATION
LACTOCOCCUS LACTIS
LISTERIA MONOCYTOGENES
MUTATED INTERNALIN A
Β-LACTOGLOBULIN
topic DENDRITIC CELLS
DNA DELIVERY
INTERNALIZATION
LACTOCOCCUS LACTIS
LISTERIA MONOCYTOGENES
MUTATED INTERNALIN A
Β-LACTOGLOBULIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Lactococcus lactis (L. lactis), a generally regarded as safe (GRAS) bacterium has recently been investigated as a mucosal delivery vehicle for DNA vaccines. Because of its GRAS status, L. lactis represents an attractive alternative to attenuated pathogens. Previous studies showed that eukaryotic expression plasmids could be delivered into intestinal epithelial cells (IECs) by L. lactis, or recombinant invasive strains of L. lactis, leading to heterologous protein expression. Although expression of antigens in IECs might lead to vaccine responses, it would be of interest to know whether uptake of L. lactis DNA vaccines by dendritic cells (DCs) could lead to antigen expression as they are unique in their ability to induce antigen-specific T cell responses. To test this, we incubated mouse bone marrow-derived DCs (BMDCs) with invasive L. lactis strains expressing either Staphylococcus aureus Fibronectin Binding Protein A (LL-FnBPA+), or Listeria monocytogenes mutated Internalin A (LL-mInlA+), both strains carrying a plasmid DNA vaccine (pValac) encoding for the cow milk allergen β-lactoglobulin (BLG). We demonstrated that they can transfect BMDCs, inducing the secretion of the pro-inflammatory cytokine IL-12. We also measured the capacity of strains to invade a polarized monolayer of IECs, mimicking the situation encountered in the gastrointestinal tract. Gentamycin survival assay in these cells showed that LL-mInlA+ is 100 times more invasive than L. lactis. The cross-talk between differentiated IECs, BMDCs and bacteria was also evaluated using an in vitro transwell co-culture model. Co-incubation of strains in this model showed that DCs incubated with LL-mInlA+ containing pValac:BLG could express significant levels of BLG. These results suggest that DCs could sample bacteria containing the DNA vaccine across the epithelial barrier and express the antigen.
Fil: de Azevedo, Marcela. Universidade Federal de Minas Gerais; Brasil
Fil: Meijerink, Marjolein. Wageningen University. Host Microbe Interactomics; Países Bajos
Fil: Taverne, Nico. Wageningen University. Host Microbe Interactomics; Países Bajos
Fil: Bastos Pereira, Vanessa. Universidade Federal de Minas Gerais; Brasil
Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
Fil: Azevedo, Vasco. Universidade Federal de Minas Gerais; Brasil
Fil: Miyoshi, Anderson. Universidade Federal de Minas Gerais; Brasil
Fil: Langella, Philippe. Institut National de la Recherche Agronomique; Francia. AgroParisTech; Francia
Fil: Wells, Jerry M.. Wageningen University. Host Microbe Interactomics; Países Bajos
Fil: Chatel, Jean Marc. Institut National de la Recherche Agronomique; Francia. AgroParisTech; Francia
description Lactococcus lactis (L. lactis), a generally regarded as safe (GRAS) bacterium has recently been investigated as a mucosal delivery vehicle for DNA vaccines. Because of its GRAS status, L. lactis represents an attractive alternative to attenuated pathogens. Previous studies showed that eukaryotic expression plasmids could be delivered into intestinal epithelial cells (IECs) by L. lactis, or recombinant invasive strains of L. lactis, leading to heterologous protein expression. Although expression of antigens in IECs might lead to vaccine responses, it would be of interest to know whether uptake of L. lactis DNA vaccines by dendritic cells (DCs) could lead to antigen expression as they are unique in their ability to induce antigen-specific T cell responses. To test this, we incubated mouse bone marrow-derived DCs (BMDCs) with invasive L. lactis strains expressing either Staphylococcus aureus Fibronectin Binding Protein A (LL-FnBPA+), or Listeria monocytogenes mutated Internalin A (LL-mInlA+), both strains carrying a plasmid DNA vaccine (pValac) encoding for the cow milk allergen β-lactoglobulin (BLG). We demonstrated that they can transfect BMDCs, inducing the secretion of the pro-inflammatory cytokine IL-12. We also measured the capacity of strains to invade a polarized monolayer of IECs, mimicking the situation encountered in the gastrointestinal tract. Gentamycin survival assay in these cells showed that LL-mInlA+ is 100 times more invasive than L. lactis. The cross-talk between differentiated IECs, BMDCs and bacteria was also evaluated using an in vitro transwell co-culture model. Co-incubation of strains in this model showed that DCs incubated with LL-mInlA+ containing pValac:BLG could express significant levels of BLG. These results suggest that DCs could sample bacteria containing the DNA vaccine across the epithelial barrier and express the antigen.
publishDate 2015
dc.date.none.fl_str_mv 2015-09-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/2910
de Azevedo, Marcela ; Meijerink, Marjolein; Taverne, Nico ; Bastos Pereira, Vanessa ; Leblanc, Jean Guy Joseph; et al.; Recombinant invasive Lactococcus lactis can transfer DNA vaccines either directly to dendritic cells or across an epithelial cell monolayer; Elsevier; Vaccine; 33; 38; 11-9-2015; 4807-4812
0264-410X
1873-2518
url http://hdl.handle.net/11336/2910
identifier_str_mv de Azevedo, Marcela ; Meijerink, Marjolein; Taverne, Nico ; Bastos Pereira, Vanessa ; Leblanc, Jean Guy Joseph; et al.; Recombinant invasive Lactococcus lactis can transfer DNA vaccines either directly to dendritic cells or across an epithelial cell monolayer; Elsevier; Vaccine; 33; 38; 11-9-2015; 4807-4812
0264-410X
1873-2518
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/journal/0264410X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vaccine.2015.07.077
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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