Expression of the hybrid bacteriocin Ent35-MccV in Lactococcus lactis and its use for controlling Listeria monocytogenes and Escherichia coli in milk

Autores
Acuña, Leonardo; Corbalan, Natalia Soledad; Quintela Baluja, Marcos; Barros Velázquez, Jorge; Bellomio, Augusto
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bacteriocins are antimicrobial peptides synthesised ribosomally. One of the main drawbacks of bacteriocins as food biopreservative agents is their restricted action spectrum. To obtain a broad-spectrum bacteriocin-producing lactic acid bacterium, we engineered a fusion protein between the enterocin P signal peptide and the broad-spectrum chimerical bacteriocin Ent35-MccV. Lactococcus lactis NZ9000 transformed with the recombinant plasmid (pRUK) was tested to identify the bacteriocin production by antimicrobial activity and colony MALDI-TOF mass spectrometry. Additionally, we performed mixed cultures in skim milk to determine the inhibition of Lc. lactis NZ9000 (pRUK) on both Listeria monocytogenes and Escherichia coli strains isolated from food. The results showed that Lc. lactis NZ9000 (pRUK) was able to diminish the growth of both L. monocytogenes and E. coli in skim milk due to Ent35-MccV production. Ent35-MccV is the first linear, genetically engineered bacteriocin produced in situ in food products used effectively to control Gram-negative and Gram-positive foodborne pathogens.
Fil: Acuña, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Corbalan, Natalia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Quintela Baluja, Marcos. Universidad de Santiago de Compostela; España
Fil: Barros Velázquez, Jorge. Universidad de Santiago de Compostela; España
Fil: Bellomio, Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Materia
Antimicrobial peptides
Lactococcus lactis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/236102
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/236102
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Expression of the hybrid bacteriocin Ent35-MccV in Lactococcus lactis and its use for controlling Listeria monocytogenes and Escherichia coli in milkAcuña, LeonardoCorbalan, Natalia SoledadQuintela Baluja, MarcosBarros Velázquez, JorgeBellomio, AugustoAntimicrobial peptidesLactococcus lactishttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Bacteriocins are antimicrobial peptides synthesised ribosomally. One of the main drawbacks of bacteriocins as food biopreservative agents is their restricted action spectrum. To obtain a broad-spectrum bacteriocin-producing lactic acid bacterium, we engineered a fusion protein between the enterocin P signal peptide and the broad-spectrum chimerical bacteriocin Ent35-MccV. Lactococcus lactis NZ9000 transformed with the recombinant plasmid (pRUK) was tested to identify the bacteriocin production by antimicrobial activity and colony MALDI-TOF mass spectrometry. Additionally, we performed mixed cultures in skim milk to determine the inhibition of Lc. lactis NZ9000 (pRUK) on both Listeria monocytogenes and Escherichia coli strains isolated from food. The results showed that Lc. lactis NZ9000 (pRUK) was able to diminish the growth of both L. monocytogenes and E. coli in skim milk due to Ent35-MccV production. Ent35-MccV is the first linear, genetically engineered bacteriocin produced in situ in food products used effectively to control Gram-negative and Gram-positive foodborne pathogens.Fil: Acuña, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Corbalan, Natalia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Quintela Baluja, Marcos. Universidad de Santiago de Compostela; EspañaFil: Barros Velázquez, Jorge. Universidad de Santiago de Compostela; EspañaFil: Bellomio, Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaElsevier2020-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/236102Acuña, Leonardo; Corbalan, Natalia Soledad; Quintela Baluja, Marcos; Barros Velázquez, Jorge; Bellomio, Augusto; Expression of the hybrid bacteriocin Ent35-MccV in Lactococcus lactis and its use for controlling Listeria monocytogenes and Escherichia coli in milk; Elsevier; International Dairy Journal; 104; 1-2020; 1-370958-6946CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0958694620300200info:eu-repo/semantics/altIdentifier/doi/10.1016/j.idairyj.2020.104650info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:03:47Zoai:ri.conicet.gov.ar:11336/236102instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:03:47.864CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Expression of the hybrid bacteriocin Ent35-MccV in Lactococcus lactis and its use for controlling Listeria monocytogenes and Escherichia coli in milk
title Expression of the hybrid bacteriocin Ent35-MccV in Lactococcus lactis and its use for controlling Listeria monocytogenes and Escherichia coli in milk
spellingShingle Expression of the hybrid bacteriocin Ent35-MccV in Lactococcus lactis and its use for controlling Listeria monocytogenes and Escherichia coli in milk
Acuña, Leonardo
Antimicrobial peptides
Lactococcus lactis
title_short Expression of the hybrid bacteriocin Ent35-MccV in Lactococcus lactis and its use for controlling Listeria monocytogenes and Escherichia coli in milk
title_full Expression of the hybrid bacteriocin Ent35-MccV in Lactococcus lactis and its use for controlling Listeria monocytogenes and Escherichia coli in milk
title_fullStr Expression of the hybrid bacteriocin Ent35-MccV in Lactococcus lactis and its use for controlling Listeria monocytogenes and Escherichia coli in milk
title_full_unstemmed Expression of the hybrid bacteriocin Ent35-MccV in Lactococcus lactis and its use for controlling Listeria monocytogenes and Escherichia coli in milk
title_sort Expression of the hybrid bacteriocin Ent35-MccV in Lactococcus lactis and its use for controlling Listeria monocytogenes and Escherichia coli in milk
dc.creator.none.fl_str_mv Acuña, Leonardo
Corbalan, Natalia Soledad
Quintela Baluja, Marcos
Barros Velázquez, Jorge
Bellomio, Augusto
author Acuña, Leonardo
author_facet Acuña, Leonardo
Corbalan, Natalia Soledad
Quintela Baluja, Marcos
Barros Velázquez, Jorge
Bellomio, Augusto
author_role author
author2 Corbalan, Natalia Soledad
Quintela Baluja, Marcos
Barros Velázquez, Jorge
Bellomio, Augusto
author2_role author
author
author
author
dc.subject.none.fl_str_mv Antimicrobial peptides
Lactococcus lactis
topic Antimicrobial peptides
Lactococcus lactis
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Bacteriocins are antimicrobial peptides synthesised ribosomally. One of the main drawbacks of bacteriocins as food biopreservative agents is their restricted action spectrum. To obtain a broad-spectrum bacteriocin-producing lactic acid bacterium, we engineered a fusion protein between the enterocin P signal peptide and the broad-spectrum chimerical bacteriocin Ent35-MccV. Lactococcus lactis NZ9000 transformed with the recombinant plasmid (pRUK) was tested to identify the bacteriocin production by antimicrobial activity and colony MALDI-TOF mass spectrometry. Additionally, we performed mixed cultures in skim milk to determine the inhibition of Lc. lactis NZ9000 (pRUK) on both Listeria monocytogenes and Escherichia coli strains isolated from food. The results showed that Lc. lactis NZ9000 (pRUK) was able to diminish the growth of both L. monocytogenes and E. coli in skim milk due to Ent35-MccV production. Ent35-MccV is the first linear, genetically engineered bacteriocin produced in situ in food products used effectively to control Gram-negative and Gram-positive foodborne pathogens.
Fil: Acuña, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Corbalan, Natalia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Quintela Baluja, Marcos. Universidad de Santiago de Compostela; España
Fil: Barros Velázquez, Jorge. Universidad de Santiago de Compostela; España
Fil: Bellomio, Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
description Bacteriocins are antimicrobial peptides synthesised ribosomally. One of the main drawbacks of bacteriocins as food biopreservative agents is their restricted action spectrum. To obtain a broad-spectrum bacteriocin-producing lactic acid bacterium, we engineered a fusion protein between the enterocin P signal peptide and the broad-spectrum chimerical bacteriocin Ent35-MccV. Lactococcus lactis NZ9000 transformed with the recombinant plasmid (pRUK) was tested to identify the bacteriocin production by antimicrobial activity and colony MALDI-TOF mass spectrometry. Additionally, we performed mixed cultures in skim milk to determine the inhibition of Lc. lactis NZ9000 (pRUK) on both Listeria monocytogenes and Escherichia coli strains isolated from food. The results showed that Lc. lactis NZ9000 (pRUK) was able to diminish the growth of both L. monocytogenes and E. coli in skim milk due to Ent35-MccV production. Ent35-MccV is the first linear, genetically engineered bacteriocin produced in situ in food products used effectively to control Gram-negative and Gram-positive foodborne pathogens.
publishDate 2020
dc.date.none.fl_str_mv 2020-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/236102
Acuña, Leonardo; Corbalan, Natalia Soledad; Quintela Baluja, Marcos; Barros Velázquez, Jorge; Bellomio, Augusto; Expression of the hybrid bacteriocin Ent35-MccV in Lactococcus lactis and its use for controlling Listeria monocytogenes and Escherichia coli in milk; Elsevier; International Dairy Journal; 104; 1-2020; 1-37
0958-6946
CONICET Digital
CONICET
url http://hdl.handle.net/11336/236102
identifier_str_mv Acuña, Leonardo; Corbalan, Natalia Soledad; Quintela Baluja, Marcos; Barros Velázquez, Jorge; Bellomio, Augusto; Expression of the hybrid bacteriocin Ent35-MccV in Lactococcus lactis and its use for controlling Listeria monocytogenes and Escherichia coli in milk; Elsevier; International Dairy Journal; 104; 1-2020; 1-37
0958-6946
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0958694620300200
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.idairyj.2020.104650
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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