Development of HPLC and UV spectrophotometric methods for the determination of ascorbic acid using hydroxypropyl-β-cyclodextrin and triethanolamine as photostabilizing agents

Autores
Garnero, Claudia; Longhi, Marcela Raquel
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this study, the effect of complex formation with triethanolamine (TEA) alone and in combination with hydroxypropyl-b-cyclodextrin (HP-b-CD) on the photostability of ascorbic acid was evaluated for exposure to artificial and diffuse daylight. The first-order rate constants for the photodegradation reactions were determined. The data obtained showed that these complexes strongly reduced the photodegradation process with an 11- and 35-fold increase in the photostability of ascorbic acid, depending of the ligand concentration and the irradiation source. The multicomponent complex gave a significantly better stabilization for exposure to light than TEA alone. Due to the fact that the complexation extended the exposure of ascorbic acid to light (without molecular changes), UV spectrophotometric and reversed phase high performance liquid chromatographic (HPLC) methods were developed for the quantitative determination of the vitamin in pure form and in pharmaceutical preparations. These methods were statistically validated, all the validation parameters were found to be within the acceptance range. These results demonstrate that the proposed methods are  suitable for the quality control of ascorbic acid, providing simple, rapid, precise, accurate and convenient approaches for routine analysis of bulk drug and pharmaceutical formulations.
Fil: Garnero, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
Fil: Longhi, Marcela Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
Materia
Ascorbic acid
Complexation
Photostabilization
UV spectrophotometry
High performance liquid chromatography
Validation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/109727
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Development of HPLC and UV spectrophotometric methods for the determination of ascorbic acid using hydroxypropyl-β-cyclodextrin and triethanolamine as photostabilizing agentsGarnero, ClaudiaLonghi, Marcela RaquelAscorbic acidComplexationPhotostabilizationUV spectrophotometryHigh performance liquid chromatographyValidationhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In this study, the effect of complex formation with triethanolamine (TEA) alone and in combination with hydroxypropyl-b-cyclodextrin (HP-b-CD) on the photostability of ascorbic acid was evaluated for exposure to artificial and diffuse daylight. The first-order rate constants for the photodegradation reactions were determined. The data obtained showed that these complexes strongly reduced the photodegradation process with an 11- and 35-fold increase in the photostability of ascorbic acid, depending of the ligand concentration and the irradiation source. The multicomponent complex gave a significantly better stabilization for exposure to light than TEA alone. Due to the fact that the complexation extended the exposure of ascorbic acid to light (without molecular changes), UV spectrophotometric and reversed phase high performance liquid chromatographic (HPLC) methods were developed for the quantitative determination of the vitamin in pure form and in pharmaceutical preparations. These methods were statistically validated, all the validation parameters were found to be within the acceptance range. These results demonstrate that the proposed methods are  suitable for the quality control of ascorbic acid, providing simple, rapid, precise, accurate and convenient approaches for routine analysis of bulk drug and pharmaceutical formulations.Fil: Garnero, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; ArgentinaFil: Longhi, Marcela Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; ArgentinaElsevier Science2010-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/109727Garnero, Claudia; Longhi, Marcela Raquel; Development of HPLC and UV spectrophotometric methods for the determination of ascorbic acid using hydroxypropyl-β-cyclodextrin and triethanolamine as photostabilizing agents; Elsevier Science; Analytica Chimica Acta; 659; 1-2; 2-2010; 159-1660003-2670CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/20103119/info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aca.2009.11.038info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:38:24Zoai:ri.conicet.gov.ar:11336/109727instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:38:25.152CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development of HPLC and UV spectrophotometric methods for the determination of ascorbic acid using hydroxypropyl-β-cyclodextrin and triethanolamine as photostabilizing agents
title Development of HPLC and UV spectrophotometric methods for the determination of ascorbic acid using hydroxypropyl-β-cyclodextrin and triethanolamine as photostabilizing agents
spellingShingle Development of HPLC and UV spectrophotometric methods for the determination of ascorbic acid using hydroxypropyl-β-cyclodextrin and triethanolamine as photostabilizing agents
Garnero, Claudia
Ascorbic acid
Complexation
Photostabilization
UV spectrophotometry
High performance liquid chromatography
Validation
title_short Development of HPLC and UV spectrophotometric methods for the determination of ascorbic acid using hydroxypropyl-β-cyclodextrin and triethanolamine as photostabilizing agents
title_full Development of HPLC and UV spectrophotometric methods for the determination of ascorbic acid using hydroxypropyl-β-cyclodextrin and triethanolamine as photostabilizing agents
title_fullStr Development of HPLC and UV spectrophotometric methods for the determination of ascorbic acid using hydroxypropyl-β-cyclodextrin and triethanolamine as photostabilizing agents
title_full_unstemmed Development of HPLC and UV spectrophotometric methods for the determination of ascorbic acid using hydroxypropyl-β-cyclodextrin and triethanolamine as photostabilizing agents
title_sort Development of HPLC and UV spectrophotometric methods for the determination of ascorbic acid using hydroxypropyl-β-cyclodextrin and triethanolamine as photostabilizing agents
dc.creator.none.fl_str_mv Garnero, Claudia
Longhi, Marcela Raquel
author Garnero, Claudia
author_facet Garnero, Claudia
Longhi, Marcela Raquel
author_role author
author2 Longhi, Marcela Raquel
author2_role author
dc.subject.none.fl_str_mv Ascorbic acid
Complexation
Photostabilization
UV spectrophotometry
High performance liquid chromatography
Validation
topic Ascorbic acid
Complexation
Photostabilization
UV spectrophotometry
High performance liquid chromatography
Validation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In this study, the effect of complex formation with triethanolamine (TEA) alone and in combination with hydroxypropyl-b-cyclodextrin (HP-b-CD) on the photostability of ascorbic acid was evaluated for exposure to artificial and diffuse daylight. The first-order rate constants for the photodegradation reactions were determined. The data obtained showed that these complexes strongly reduced the photodegradation process with an 11- and 35-fold increase in the photostability of ascorbic acid, depending of the ligand concentration and the irradiation source. The multicomponent complex gave a significantly better stabilization for exposure to light than TEA alone. Due to the fact that the complexation extended the exposure of ascorbic acid to light (without molecular changes), UV spectrophotometric and reversed phase high performance liquid chromatographic (HPLC) methods were developed for the quantitative determination of the vitamin in pure form and in pharmaceutical preparations. These methods were statistically validated, all the validation parameters were found to be within the acceptance range. These results demonstrate that the proposed methods are  suitable for the quality control of ascorbic acid, providing simple, rapid, precise, accurate and convenient approaches for routine analysis of bulk drug and pharmaceutical formulations.
Fil: Garnero, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
Fil: Longhi, Marcela Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
description In this study, the effect of complex formation with triethanolamine (TEA) alone and in combination with hydroxypropyl-b-cyclodextrin (HP-b-CD) on the photostability of ascorbic acid was evaluated for exposure to artificial and diffuse daylight. The first-order rate constants for the photodegradation reactions were determined. The data obtained showed that these complexes strongly reduced the photodegradation process with an 11- and 35-fold increase in the photostability of ascorbic acid, depending of the ligand concentration and the irradiation source. The multicomponent complex gave a significantly better stabilization for exposure to light than TEA alone. Due to the fact that the complexation extended the exposure of ascorbic acid to light (without molecular changes), UV spectrophotometric and reversed phase high performance liquid chromatographic (HPLC) methods were developed for the quantitative determination of the vitamin in pure form and in pharmaceutical preparations. These methods were statistically validated, all the validation parameters were found to be within the acceptance range. These results demonstrate that the proposed methods are  suitable for the quality control of ascorbic acid, providing simple, rapid, precise, accurate and convenient approaches for routine analysis of bulk drug and pharmaceutical formulations.
publishDate 2010
dc.date.none.fl_str_mv 2010-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/109727
Garnero, Claudia; Longhi, Marcela Raquel; Development of HPLC and UV spectrophotometric methods for the determination of ascorbic acid using hydroxypropyl-β-cyclodextrin and triethanolamine as photostabilizing agents; Elsevier Science; Analytica Chimica Acta; 659; 1-2; 2-2010; 159-166
0003-2670
CONICET Digital
CONICET
url http://hdl.handle.net/11336/109727
identifier_str_mv Garnero, Claudia; Longhi, Marcela Raquel; Development of HPLC and UV spectrophotometric methods for the determination of ascorbic acid using hydroxypropyl-β-cyclodextrin and triethanolamine as photostabilizing agents; Elsevier Science; Analytica Chimica Acta; 659; 1-2; 2-2010; 159-166
0003-2670
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/20103119/
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aca.2009.11.038
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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