Milk fat content measurement by a simple UV spectrophotometric method: an alternative screening method

Autores
Forcato, Diego Oscar; Carmine, Mariela; Echeverría, G. E.; Pécora, R. P.; Kivatinitz, Silvia Clara
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We developed a simple microtechnique to measure lipids in milk by UV spectrophotometry. This technique is based upon the property of fatty acids to absorb UV light proportional to their concentration. Samples of powdered or fluid milk (30 or 60 µL) are added to 3 mL of analytic grade ethanol and stored at −20°C during at least 1 hr. This procedure precipitates proteins and hydrophobic peptides that interfere with UV measurement. Sample absorbances are then measured at 208 nm in an UV-Vis spectrophotometer. This technique correlated very well against Milko-Scan , a device that measures milk fat by IR spectroscopy, with a r2 > 0.982. Accuracy and precision, evaluated by recovery and replicate assays, are also very acceptable. This method is suitable as a fast, cost-effective alternative screening method to estimate milk fat content in small samples without prior lipid extraction.
Fil: Forcato, Diego Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Carmine, Mariela. Universidad Nacional de Córdoba. Facultad de Cs.exactas Físicas y Naturales. Departamento de Química Industrial y Aplicada; Argentina
Fil: Echeverría, G. E.. Fundación Escuela Superior Integral de Lechería; Argentina
Fil: Pécora, R. P.. Universidad Nacional de Córdoba. Facultad de Cs.exactas Físicas y Naturales. Departamento de Química Industrial y Aplicada; Argentina
Fil: Kivatinitz, Silvia Clara. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina
Materia
MILK
UV-SPECTROPHOTOMETRY
LIPID
FAT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/241876
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Milk fat content measurement by a simple UV spectrophotometric method: an alternative screening methodForcato, Diego OscarCarmine, MarielaEcheverría, G. E.Pécora, R. P.Kivatinitz, Silvia ClaraMILKUV-SPECTROPHOTOMETRYLIPIDFAThttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1We developed a simple microtechnique to measure lipids in milk by UV spectrophotometry. This technique is based upon the property of fatty acids to absorb UV light proportional to their concentration. Samples of powdered or fluid milk (30 or 60 µL) are added to 3 mL of analytic grade ethanol and stored at −20°C during at least 1 hr. This procedure precipitates proteins and hydrophobic peptides that interfere with UV measurement. Sample absorbances are then measured at 208 nm in an UV-Vis spectrophotometer. This technique correlated very well against Milko-Scan , a device that measures milk fat by IR spectroscopy, with a r2 > 0.982. Accuracy and precision, evaluated by recovery and replicate assays, are also very acceptable. This method is suitable as a fast, cost-effective alternative screening method to estimate milk fat content in small samples without prior lipid extraction.Fil: Forcato, Diego Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Carmine, Mariela. Universidad Nacional de Córdoba. Facultad de Cs.exactas Físicas y Naturales. Departamento de Química Industrial y Aplicada; ArgentinaFil: Echeverría, G. E.. Fundación Escuela Superior Integral de Lechería; ArgentinaFil: Pécora, R. P.. Universidad Nacional de Córdoba. Facultad de Cs.exactas Físicas y Naturales. Departamento de Química Industrial y Aplicada; ArgentinaFil: Kivatinitz, Silvia Clara. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; ArgentinaAmerican Dairy Science Association2005-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/241876Forcato, Diego Oscar; Carmine, Mariela; Echeverría, G. E.; Pécora, R. P.; Kivatinitz, Silvia Clara; Milk fat content measurement by a simple UV spectrophotometric method: an alternative screening method; American Dairy Science Association; Journal of Dairy Science; 88; 2; 2-2005; 478-4810022-0302CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0022030205727090info:eu-repo/semantics/altIdentifier/doi/10.3168/jds.S0022-0302(05)72709-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:36:34Zoai:ri.conicet.gov.ar:11336/241876instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:36:34.716CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Milk fat content measurement by a simple UV spectrophotometric method: an alternative screening method
title Milk fat content measurement by a simple UV spectrophotometric method: an alternative screening method
spellingShingle Milk fat content measurement by a simple UV spectrophotometric method: an alternative screening method
Forcato, Diego Oscar
MILK
UV-SPECTROPHOTOMETRY
LIPID
FAT
title_short Milk fat content measurement by a simple UV spectrophotometric method: an alternative screening method
title_full Milk fat content measurement by a simple UV spectrophotometric method: an alternative screening method
title_fullStr Milk fat content measurement by a simple UV spectrophotometric method: an alternative screening method
title_full_unstemmed Milk fat content measurement by a simple UV spectrophotometric method: an alternative screening method
title_sort Milk fat content measurement by a simple UV spectrophotometric method: an alternative screening method
dc.creator.none.fl_str_mv Forcato, Diego Oscar
Carmine, Mariela
Echeverría, G. E.
Pécora, R. P.
Kivatinitz, Silvia Clara
author Forcato, Diego Oscar
author_facet Forcato, Diego Oscar
Carmine, Mariela
Echeverría, G. E.
Pécora, R. P.
Kivatinitz, Silvia Clara
author_role author
author2 Carmine, Mariela
Echeverría, G. E.
Pécora, R. P.
Kivatinitz, Silvia Clara
author2_role author
author
author
author
dc.subject.none.fl_str_mv MILK
UV-SPECTROPHOTOMETRY
LIPID
FAT
topic MILK
UV-SPECTROPHOTOMETRY
LIPID
FAT
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We developed a simple microtechnique to measure lipids in milk by UV spectrophotometry. This technique is based upon the property of fatty acids to absorb UV light proportional to their concentration. Samples of powdered or fluid milk (30 or 60 µL) are added to 3 mL of analytic grade ethanol and stored at −20°C during at least 1 hr. This procedure precipitates proteins and hydrophobic peptides that interfere with UV measurement. Sample absorbances are then measured at 208 nm in an UV-Vis spectrophotometer. This technique correlated very well against Milko-Scan , a device that measures milk fat by IR spectroscopy, with a r2 > 0.982. Accuracy and precision, evaluated by recovery and replicate assays, are also very acceptable. This method is suitable as a fast, cost-effective alternative screening method to estimate milk fat content in small samples without prior lipid extraction.
Fil: Forcato, Diego Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Carmine, Mariela. Universidad Nacional de Córdoba. Facultad de Cs.exactas Físicas y Naturales. Departamento de Química Industrial y Aplicada; Argentina
Fil: Echeverría, G. E.. Fundación Escuela Superior Integral de Lechería; Argentina
Fil: Pécora, R. P.. Universidad Nacional de Córdoba. Facultad de Cs.exactas Físicas y Naturales. Departamento de Química Industrial y Aplicada; Argentina
Fil: Kivatinitz, Silvia Clara. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina
description We developed a simple microtechnique to measure lipids in milk by UV spectrophotometry. This technique is based upon the property of fatty acids to absorb UV light proportional to their concentration. Samples of powdered or fluid milk (30 or 60 µL) are added to 3 mL of analytic grade ethanol and stored at −20°C during at least 1 hr. This procedure precipitates proteins and hydrophobic peptides that interfere with UV measurement. Sample absorbances are then measured at 208 nm in an UV-Vis spectrophotometer. This technique correlated very well against Milko-Scan , a device that measures milk fat by IR spectroscopy, with a r2 > 0.982. Accuracy and precision, evaluated by recovery and replicate assays, are also very acceptable. This method is suitable as a fast, cost-effective alternative screening method to estimate milk fat content in small samples without prior lipid extraction.
publishDate 2005
dc.date.none.fl_str_mv 2005-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/241876
Forcato, Diego Oscar; Carmine, Mariela; Echeverría, G. E.; Pécora, R. P.; Kivatinitz, Silvia Clara; Milk fat content measurement by a simple UV spectrophotometric method: an alternative screening method; American Dairy Science Association; Journal of Dairy Science; 88; 2; 2-2005; 478-481
0022-0302
CONICET Digital
CONICET
url http://hdl.handle.net/11336/241876
identifier_str_mv Forcato, Diego Oscar; Carmine, Mariela; Echeverría, G. E.; Pécora, R. P.; Kivatinitz, Silvia Clara; Milk fat content measurement by a simple UV spectrophotometric method: an alternative screening method; American Dairy Science Association; Journal of Dairy Science; 88; 2; 2-2005; 478-481
0022-0302
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0022030205727090
info:eu-repo/semantics/altIdentifier/doi/10.3168/jds.S0022-0302(05)72709-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Dairy Science Association
publisher.none.fl_str_mv American Dairy Science Association
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614386394595328
score 13.069144

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