A derivative UV spectrophotometry method for the determination of levothyroxine sodium in tablets
- Autores
- Gregorini, Anabella; Ruiz, María Esperanza; Volonté, Maria Guillermina
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- A derivative UV (DUV) spectrophotometric method was developed for the determination of Levothyroxine Sodium (LT4) in tablets of different doses. Quantification was performed using the second derivative of the absorption spectrum at 253 nm (2D253) in methanol : water (50 : 50; v/v) (pH 11.2). The method was validated and compared with an HPLC procedure carried out using a RP18 column (125 x~ 4 mm, 5 microm) and methanol: phosphoric acid (0.1%) (70 : 30, v/v) (pH 3) as mobile phase. Flow rate was set at 1.5 mL/min, and detection was performed at 225 nm. The proposed DUV method was linear in the range 3.0 - 40.0 microg/mL with an appropriate precision and accuracy, and it was selective for the drug under study. On the other hand, results obtained by 2D253 analysis were similar to those obtained by HPLC, with no statistically significant differences between them. Therefore, it was concluded that the developed method is suitable for the determination of LT4 in tablets at the tested doses.
Fil: Gregorini, Anabella. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Cs.biologicas. Catedra de Control de Calidad de Medicamentos; Argentina
Fil: Ruiz, María Esperanza. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Cs.biologicas. Catedra de Control de Calidad de Medicamentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas; Argentina
Fil: Volonté, Maria Guillermina. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Cs.biologicas. Catedra de Control de Calidad de Medicamentos; Argentina - Materia
-
Levothyroxine Sodium
Derivative Uv Spectrophotometry
High Performance Liquid Chromatography - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/4880
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A derivative UV spectrophotometry method for the determination of levothyroxine sodium in tabletsGregorini, AnabellaRuiz, María EsperanzaVolonté, Maria GuillerminaLevothyroxine SodiumDerivative Uv SpectrophotometryHigh Performance Liquid Chromatographyhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A derivative UV (DUV) spectrophotometric method was developed for the determination of Levothyroxine Sodium (LT4) in tablets of different doses. Quantification was performed using the second derivative of the absorption spectrum at 253 nm (2D253) in methanol : water (50 : 50; v/v) (pH 11.2). The method was validated and compared with an HPLC procedure carried out using a RP18 column (125 x~ 4 mm, 5 microm) and methanol: phosphoric acid (0.1%) (70 : 30, v/v) (pH 3) as mobile phase. Flow rate was set at 1.5 mL/min, and detection was performed at 225 nm. The proposed DUV method was linear in the range 3.0 - 40.0 microg/mL with an appropriate precision and accuracy, and it was selective for the drug under study. On the other hand, results obtained by 2D253 analysis were similar to those obtained by HPLC, with no statistically significant differences between them. Therefore, it was concluded that the developed method is suitable for the determination of LT4 in tablets at the tested doses.Fil: Gregorini, Anabella. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Cs.biologicas. Catedra de Control de Calidad de Medicamentos; ArgentinaFil: Ruiz, María Esperanza. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Cs.biologicas. Catedra de Control de Calidad de Medicamentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas; ArgentinaFil: Volonté, Maria Guillermina. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Cs.biologicas. Catedra de Control de Calidad de Medicamentos; ArgentinaSP MAIK Nauka/Interperiodica2013-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4880Gregorini, Anabella; Ruiz, María Esperanza; Volonté, Maria Guillermina; A derivative UV spectrophotometry method for the determination of levothyroxine sodium in tablets; SP MAIK Nauka/Interperiodica; Journal Of Analytical Chemistry; 68; 6; 6-2013; 510-5151061-9348enginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1134/S1061934813060075info:eu-repo/semantics/altIdentifier/doi/10.1134/S1061934813060075info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:10:30Zoai:ri.conicet.gov.ar:11336/4880instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:10:30.978CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
A derivative UV spectrophotometry method for the determination of levothyroxine sodium in tablets |
title |
A derivative UV spectrophotometry method for the determination of levothyroxine sodium in tablets |
spellingShingle |
A derivative UV spectrophotometry method for the determination of levothyroxine sodium in tablets Gregorini, Anabella Levothyroxine Sodium Derivative Uv Spectrophotometry High Performance Liquid Chromatography |
title_short |
A derivative UV spectrophotometry method for the determination of levothyroxine sodium in tablets |
title_full |
A derivative UV spectrophotometry method for the determination of levothyroxine sodium in tablets |
title_fullStr |
A derivative UV spectrophotometry method for the determination of levothyroxine sodium in tablets |
title_full_unstemmed |
A derivative UV spectrophotometry method for the determination of levothyroxine sodium in tablets |
title_sort |
A derivative UV spectrophotometry method for the determination of levothyroxine sodium in tablets |
dc.creator.none.fl_str_mv |
Gregorini, Anabella Ruiz, María Esperanza Volonté, Maria Guillermina |
author |
Gregorini, Anabella |
author_facet |
Gregorini, Anabella Ruiz, María Esperanza Volonté, Maria Guillermina |
author_role |
author |
author2 |
Ruiz, María Esperanza Volonté, Maria Guillermina |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Levothyroxine Sodium Derivative Uv Spectrophotometry High Performance Liquid Chromatography |
topic |
Levothyroxine Sodium Derivative Uv Spectrophotometry High Performance Liquid Chromatography |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
A derivative UV (DUV) spectrophotometric method was developed for the determination of Levothyroxine Sodium (LT4) in tablets of different doses. Quantification was performed using the second derivative of the absorption spectrum at 253 nm (2D253) in methanol : water (50 : 50; v/v) (pH 11.2). The method was validated and compared with an HPLC procedure carried out using a RP18 column (125 x~ 4 mm, 5 microm) and methanol: phosphoric acid (0.1%) (70 : 30, v/v) (pH 3) as mobile phase. Flow rate was set at 1.5 mL/min, and detection was performed at 225 nm. The proposed DUV method was linear in the range 3.0 - 40.0 microg/mL with an appropriate precision and accuracy, and it was selective for the drug under study. On the other hand, results obtained by 2D253 analysis were similar to those obtained by HPLC, with no statistically significant differences between them. Therefore, it was concluded that the developed method is suitable for the determination of LT4 in tablets at the tested doses. Fil: Gregorini, Anabella. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Cs.biologicas. Catedra de Control de Calidad de Medicamentos; Argentina Fil: Ruiz, María Esperanza. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Cs.biologicas. Catedra de Control de Calidad de Medicamentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas; Argentina Fil: Volonté, Maria Guillermina. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Cs.biologicas. Catedra de Control de Calidad de Medicamentos; Argentina |
description |
A derivative UV (DUV) spectrophotometric method was developed for the determination of Levothyroxine Sodium (LT4) in tablets of different doses. Quantification was performed using the second derivative of the absorption spectrum at 253 nm (2D253) in methanol : water (50 : 50; v/v) (pH 11.2). The method was validated and compared with an HPLC procedure carried out using a RP18 column (125 x~ 4 mm, 5 microm) and methanol: phosphoric acid (0.1%) (70 : 30, v/v) (pH 3) as mobile phase. Flow rate was set at 1.5 mL/min, and detection was performed at 225 nm. The proposed DUV method was linear in the range 3.0 - 40.0 microg/mL with an appropriate precision and accuracy, and it was selective for the drug under study. On the other hand, results obtained by 2D253 analysis were similar to those obtained by HPLC, with no statistically significant differences between them. Therefore, it was concluded that the developed method is suitable for the determination of LT4 in tablets at the tested doses. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-06 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/4880 Gregorini, Anabella; Ruiz, María Esperanza; Volonté, Maria Guillermina; A derivative UV spectrophotometry method for the determination of levothyroxine sodium in tablets; SP MAIK Nauka/Interperiodica; Journal Of Analytical Chemistry; 68; 6; 6-2013; 510-515 1061-9348 |
url |
http://hdl.handle.net/11336/4880 |
identifier_str_mv |
Gregorini, Anabella; Ruiz, María Esperanza; Volonté, Maria Guillermina; A derivative UV spectrophotometry method for the determination of levothyroxine sodium in tablets; SP MAIK Nauka/Interperiodica; Journal Of Analytical Chemistry; 68; 6; 6-2013; 510-515 1061-9348 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1134/S1061934813060075 info:eu-repo/semantics/altIdentifier/doi/10.1134/S1061934813060075 info:eu-repo/semantics/altIdentifier/doi/ |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
SP MAIK Nauka/Interperiodica |
publisher.none.fl_str_mv |
SP MAIK Nauka/Interperiodica |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613995157258240 |
score |
13.070432 |