Mitochondrial peroxynitrite generation is mainly driven by superoxide steady-state concentration rather than by nitric oxide steady-state concentration

Autores
Valdez, Laura Batriz; Bombicino, Silvina Sonia; Iglesias, Dario Ezequiel; Rukavina Mikusic, Ivana Agustina; Boveris, Alberto Antonio
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In biological systems, ONOO- production depends on production rates of NO and O2-, and on the reactions of these two free radicals with other biological components, which limit the local concentrations of NO and O2-. In mitochondria, O2- is generated through the autoxidation of semiquinones at Complexes I and III, and it may suffer the SOD-catalysed dismutation reaction to produce H2O2 or react with NO in a classical termination reaction between free radicals. These diffusion-controlled reactions kinetically compete for O2- degradation. Results from our laboratory have shown that even in physiopathological situations in which NO production is reduced, such as the mitochondrial dysfunction associated to stunned heart, mitochondrial ONOO- production rate may be slightly increased if the steady-state concentration of O2- is augmented. The enhancement in O2- concentration leads to an increase in its degradation by reaction with NO, decreasing NO bioavailability and increasing ONOO- production rate. Therefore, mitochondrial ONOO- generation is mainly driven by O2- rather than by NO steady-state concentrations. In this scenario, the switch from signalling pathways of NO to oxidative damage takes place and oxidation reactions of biomolecules or modification of proteins by nitration may occur.
Fil: Valdez, Laura Batriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Bombicino, Silvina Sonia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Iglesias, Dario Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Rukavina Mikusic, Ivana Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Boveris, Alberto Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Materia
MITOCHONDRIA
PEROXINITRITE
NITRIC OXIDE
SUPEROXIDE
STEADY-STATES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/90570
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Mitochondrial peroxynitrite generation is mainly driven by superoxide steady-state concentration rather than by nitric oxide steady-state concentrationValdez, Laura BatrizBombicino, Silvina SoniaIglesias, Dario EzequielRukavina Mikusic, Ivana AgustinaBoveris, Alberto AntonioMITOCHONDRIAPEROXINITRITENITRIC OXIDESUPEROXIDESTEADY-STATEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In biological systems, ONOO- production depends on production rates of NO and O2-, and on the reactions of these two free radicals with other biological components, which limit the local concentrations of NO and O2-. In mitochondria, O2- is generated through the autoxidation of semiquinones at Complexes I and III, and it may suffer the SOD-catalysed dismutation reaction to produce H2O2 or react with NO in a classical termination reaction between free radicals. These diffusion-controlled reactions kinetically compete for O2- degradation. Results from our laboratory have shown that even in physiopathological situations in which NO production is reduced, such as the mitochondrial dysfunction associated to stunned heart, mitochondrial ONOO- production rate may be slightly increased if the steady-state concentration of O2- is augmented. The enhancement in O2- concentration leads to an increase in its degradation by reaction with NO, decreasing NO bioavailability and increasing ONOO- production rate. Therefore, mitochondrial ONOO- generation is mainly driven by O2- rather than by NO steady-state concentrations. In this scenario, the switch from signalling pathways of NO to oxidative damage takes place and oxidation reactions of biomolecules or modification of proteins by nitration may occur.Fil: Valdez, Laura Batriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Bombicino, Silvina Sonia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Iglesias, Dario Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Rukavina Mikusic, Ivana Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Boveris, Alberto Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaMedCrave Group2018-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/90570Valdez, Laura Batriz; Bombicino, Silvina Sonia; Iglesias, Dario Ezequiel; Rukavina Mikusic, Ivana Agustina; Boveris, Alberto Antonio; Mitochondrial peroxynitrite generation is mainly driven by superoxide steady-state concentration rather than by nitric oxide steady-state concentration ; MedCrave Group; International Journal of Molecular Biology; 3; 2; 3-2018; 58-632573-2889CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://medcraveonline.com/IJMBOA/mitochondrial-peroxynitrite-generation-is-mainly-driven-by-superoxide-steady-state-concentration-rather-than-by-nitric-oxide-steady-state-concentration.htmlinfo:eu-repo/semantics/altIdentifier/doi/10.15406/ijmboa.2018.03.00051info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:27:07Zoai:ri.conicet.gov.ar:11336/90570instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:27:08.276CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mitochondrial peroxynitrite generation is mainly driven by superoxide steady-state concentration rather than by nitric oxide steady-state concentration
title Mitochondrial peroxynitrite generation is mainly driven by superoxide steady-state concentration rather than by nitric oxide steady-state concentration
spellingShingle Mitochondrial peroxynitrite generation is mainly driven by superoxide steady-state concentration rather than by nitric oxide steady-state concentration
Valdez, Laura Batriz
MITOCHONDRIA
PEROXINITRITE
NITRIC OXIDE
SUPEROXIDE
STEADY-STATES
title_short Mitochondrial peroxynitrite generation is mainly driven by superoxide steady-state concentration rather than by nitric oxide steady-state concentration
title_full Mitochondrial peroxynitrite generation is mainly driven by superoxide steady-state concentration rather than by nitric oxide steady-state concentration
title_fullStr Mitochondrial peroxynitrite generation is mainly driven by superoxide steady-state concentration rather than by nitric oxide steady-state concentration
title_full_unstemmed Mitochondrial peroxynitrite generation is mainly driven by superoxide steady-state concentration rather than by nitric oxide steady-state concentration
title_sort Mitochondrial peroxynitrite generation is mainly driven by superoxide steady-state concentration rather than by nitric oxide steady-state concentration
dc.creator.none.fl_str_mv Valdez, Laura Batriz
Bombicino, Silvina Sonia
Iglesias, Dario Ezequiel
Rukavina Mikusic, Ivana Agustina
Boveris, Alberto Antonio
author Valdez, Laura Batriz
author_facet Valdez, Laura Batriz
Bombicino, Silvina Sonia
Iglesias, Dario Ezequiel
Rukavina Mikusic, Ivana Agustina
Boveris, Alberto Antonio
author_role author
author2 Bombicino, Silvina Sonia
Iglesias, Dario Ezequiel
Rukavina Mikusic, Ivana Agustina
Boveris, Alberto Antonio
author2_role author
author
author
author
dc.subject.none.fl_str_mv MITOCHONDRIA
PEROXINITRITE
NITRIC OXIDE
SUPEROXIDE
STEADY-STATES
topic MITOCHONDRIA
PEROXINITRITE
NITRIC OXIDE
SUPEROXIDE
STEADY-STATES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In biological systems, ONOO- production depends on production rates of NO and O2-, and on the reactions of these two free radicals with other biological components, which limit the local concentrations of NO and O2-. In mitochondria, O2- is generated through the autoxidation of semiquinones at Complexes I and III, and it may suffer the SOD-catalysed dismutation reaction to produce H2O2 or react with NO in a classical termination reaction between free radicals. These diffusion-controlled reactions kinetically compete for O2- degradation. Results from our laboratory have shown that even in physiopathological situations in which NO production is reduced, such as the mitochondrial dysfunction associated to stunned heart, mitochondrial ONOO- production rate may be slightly increased if the steady-state concentration of O2- is augmented. The enhancement in O2- concentration leads to an increase in its degradation by reaction with NO, decreasing NO bioavailability and increasing ONOO- production rate. Therefore, mitochondrial ONOO- generation is mainly driven by O2- rather than by NO steady-state concentrations. In this scenario, the switch from signalling pathways of NO to oxidative damage takes place and oxidation reactions of biomolecules or modification of proteins by nitration may occur.
Fil: Valdez, Laura Batriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Bombicino, Silvina Sonia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Iglesias, Dario Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Rukavina Mikusic, Ivana Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Boveris, Alberto Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
description In biological systems, ONOO- production depends on production rates of NO and O2-, and on the reactions of these two free radicals with other biological components, which limit the local concentrations of NO and O2-. In mitochondria, O2- is generated through the autoxidation of semiquinones at Complexes I and III, and it may suffer the SOD-catalysed dismutation reaction to produce H2O2 or react with NO in a classical termination reaction between free radicals. These diffusion-controlled reactions kinetically compete for O2- degradation. Results from our laboratory have shown that even in physiopathological situations in which NO production is reduced, such as the mitochondrial dysfunction associated to stunned heart, mitochondrial ONOO- production rate may be slightly increased if the steady-state concentration of O2- is augmented. The enhancement in O2- concentration leads to an increase in its degradation by reaction with NO, decreasing NO bioavailability and increasing ONOO- production rate. Therefore, mitochondrial ONOO- generation is mainly driven by O2- rather than by NO steady-state concentrations. In this scenario, the switch from signalling pathways of NO to oxidative damage takes place and oxidation reactions of biomolecules or modification of proteins by nitration may occur.
publishDate 2018
dc.date.none.fl_str_mv 2018-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/90570
Valdez, Laura Batriz; Bombicino, Silvina Sonia; Iglesias, Dario Ezequiel; Rukavina Mikusic, Ivana Agustina; Boveris, Alberto Antonio; Mitochondrial peroxynitrite generation is mainly driven by superoxide steady-state concentration rather than by nitric oxide steady-state concentration ; MedCrave Group; International Journal of Molecular Biology; 3; 2; 3-2018; 58-63
2573-2889
CONICET Digital
CONICET
url http://hdl.handle.net/11336/90570
identifier_str_mv Valdez, Laura Batriz; Bombicino, Silvina Sonia; Iglesias, Dario Ezequiel; Rukavina Mikusic, Ivana Agustina; Boveris, Alberto Antonio; Mitochondrial peroxynitrite generation is mainly driven by superoxide steady-state concentration rather than by nitric oxide steady-state concentration ; MedCrave Group; International Journal of Molecular Biology; 3; 2; 3-2018; 58-63
2573-2889
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://medcraveonline.com/IJMBOA/mitochondrial-peroxynitrite-generation-is-mainly-driven-by-superoxide-steady-state-concentration-rather-than-by-nitric-oxide-steady-state-concentration.html
info:eu-repo/semantics/altIdentifier/doi/10.15406/ijmboa.2018.03.00051
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MedCrave Group
publisher.none.fl_str_mv MedCrave Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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