Bioactivity and action mechanism of green propolis against Pythium aphanidermatum

Autores
Moreira Pazin, Wallance; Santos, Suikinai N. Dos; Queiroz, Sonia C. N.; Bagatolli, Luis Alberto; Soares, Ademilson E. E.; Melo, Itamar S. de; Ito, Amando S.
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We have established how natural compounds from green propolis collected by the species Apis mellifera act against the growth of Pythium aphanidermatum. On the basis of mass spectrometry (Q-ToF MS), we determined that Artepillin C, the major constituent of green propolis, underlies the effect and displays activity against P. aphanidermatum at a minimal inhibitory concentration of 750 µg.mL-1. Biophysical studies based on model membranes showed that this inhibitory effect may be linked with a membrane-related phenomenon: Artepillin C increases the permeability of membranes with relatively high fluidity in their lateral structure, a feature that is in line with the lipid composition reported for the cytoplasmic membrane of P. aphanidermatum. Therefore, the present study supports the use of the effective and inexpensive green propolis to control the impact of the dangerous phytopathogen P. aphanidermatum on agriculture.
Fil: Moreira Pazin, Wallance. Universidade de Sao Paulo; Brasil
Fil: Santos, Suikinai N. Dos. Laboratório de Microbiologia Ambiental; Brasil
Fil: Queiroz, Sonia C. N.. Laboratório de Microbiologia Ambiental; Brasil
Fil: Bagatolli, Luis Alberto. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Soares, Ademilson E. E.. Universidade de Sao Paulo; Brasil
Fil: Melo, Itamar S. de. Laboratório de Microbiologia Ambiental; Brasil
Fil: Ito, Amando S.. Universidade de Sao Paulo; Brasil
Materia
ARTEPILLIN C
FUNGICIDE
GREEN PROPOLIS
MODEL MEMBRANES
PYTHIUM APHANIDERMATUM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/128584
Acceder
id CONICETDig_8654561eb5556f1742402a73237a6d84
oai_identifier_str oai:ri.conicet.gov.ar:11336/128584
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Bioactivity and action mechanism of green propolis against Pythium aphanidermatumMoreira Pazin, WallanceSantos, Suikinai N. DosQueiroz, Sonia C. N.Bagatolli, Luis AlbertoSoares, Ademilson E. E.Melo, Itamar S. deIto, Amando S.ARTEPILLIN CFUNGICIDEGREEN PROPOLISMODEL MEMBRANESPYTHIUM APHANIDERMATUMhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We have established how natural compounds from green propolis collected by the species Apis mellifera act against the growth of Pythium aphanidermatum. On the basis of mass spectrometry (Q-ToF MS), we determined that Artepillin C, the major constituent of green propolis, underlies the effect and displays activity against P. aphanidermatum at a minimal inhibitory concentration of 750 µg.mL-1. Biophysical studies based on model membranes showed that this inhibitory effect may be linked with a membrane-related phenomenon: Artepillin C increases the permeability of membranes with relatively high fluidity in their lateral structure, a feature that is in line with the lipid composition reported for the cytoplasmic membrane of P. aphanidermatum. Therefore, the present study supports the use of the effective and inexpensive green propolis to control the impact of the dangerous phytopathogen P. aphanidermatum on agriculture.Fil: Moreira Pazin, Wallance. Universidade de Sao Paulo; BrasilFil: Santos, Suikinai N. Dos. Laboratório de Microbiologia Ambiental; BrasilFil: Queiroz, Sonia C. N.. Laboratório de Microbiologia Ambiental; BrasilFil: Bagatolli, Luis Alberto. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaFil: Soares, Ademilson E. E.. Universidade de Sao Paulo; BrasilFil: Melo, Itamar S. de. Laboratório de Microbiologia Ambiental; BrasilFil: Ito, Amando S.. Universidade de Sao Paulo; BrasilAcademia Brasileira de Ciencias2019-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/128584Moreira Pazin, Wallance; Santos, Suikinai N. Dos; Queiroz, Sonia C. N.; Bagatolli, Luis Alberto; Soares, Ademilson E. E.; et al.; Bioactivity and action mechanism of green propolis against Pythium aphanidermatum; Academia Brasileira de Ciencias; Anais da Academia Brasileira de Ciencias; 91; 2; 7-2019; 1-91678-2690CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1590/0001-3765201920180598info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/w5m3skinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:50:42Zoai:ri.conicet.gov.ar:11336/128584instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:50:42.565CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bioactivity and action mechanism of green propolis against Pythium aphanidermatum
title Bioactivity and action mechanism of green propolis against Pythium aphanidermatum
spellingShingle Bioactivity and action mechanism of green propolis against Pythium aphanidermatum
Moreira Pazin, Wallance
ARTEPILLIN C
FUNGICIDE
GREEN PROPOLIS
MODEL MEMBRANES
PYTHIUM APHANIDERMATUM
title_short Bioactivity and action mechanism of green propolis against Pythium aphanidermatum
title_full Bioactivity and action mechanism of green propolis against Pythium aphanidermatum
title_fullStr Bioactivity and action mechanism of green propolis against Pythium aphanidermatum
title_full_unstemmed Bioactivity and action mechanism of green propolis against Pythium aphanidermatum
title_sort Bioactivity and action mechanism of green propolis against Pythium aphanidermatum
dc.creator.none.fl_str_mv Moreira Pazin, Wallance
Santos, Suikinai N. Dos
Queiroz, Sonia C. N.
Bagatolli, Luis Alberto
Soares, Ademilson E. E.
Melo, Itamar S. de
Ito, Amando S.
author Moreira Pazin, Wallance
author_facet Moreira Pazin, Wallance
Santos, Suikinai N. Dos
Queiroz, Sonia C. N.
Bagatolli, Luis Alberto
Soares, Ademilson E. E.
Melo, Itamar S. de
Ito, Amando S.
author_role author
author2 Santos, Suikinai N. Dos
Queiroz, Sonia C. N.
Bagatolli, Luis Alberto
Soares, Ademilson E. E.
Melo, Itamar S. de
Ito, Amando S.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ARTEPILLIN C
FUNGICIDE
GREEN PROPOLIS
MODEL MEMBRANES
PYTHIUM APHANIDERMATUM
topic ARTEPILLIN C
FUNGICIDE
GREEN PROPOLIS
MODEL MEMBRANES
PYTHIUM APHANIDERMATUM
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We have established how natural compounds from green propolis collected by the species Apis mellifera act against the growth of Pythium aphanidermatum. On the basis of mass spectrometry (Q-ToF MS), we determined that Artepillin C, the major constituent of green propolis, underlies the effect and displays activity against P. aphanidermatum at a minimal inhibitory concentration of 750 µg.mL-1. Biophysical studies based on model membranes showed that this inhibitory effect may be linked with a membrane-related phenomenon: Artepillin C increases the permeability of membranes with relatively high fluidity in their lateral structure, a feature that is in line with the lipid composition reported for the cytoplasmic membrane of P. aphanidermatum. Therefore, the present study supports the use of the effective and inexpensive green propolis to control the impact of the dangerous phytopathogen P. aphanidermatum on agriculture.
Fil: Moreira Pazin, Wallance. Universidade de Sao Paulo; Brasil
Fil: Santos, Suikinai N. Dos. Laboratório de Microbiologia Ambiental; Brasil
Fil: Queiroz, Sonia C. N.. Laboratório de Microbiologia Ambiental; Brasil
Fil: Bagatolli, Luis Alberto. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Soares, Ademilson E. E.. Universidade de Sao Paulo; Brasil
Fil: Melo, Itamar S. de. Laboratório de Microbiologia Ambiental; Brasil
Fil: Ito, Amando S.. Universidade de Sao Paulo; Brasil
description We have established how natural compounds from green propolis collected by the species Apis mellifera act against the growth of Pythium aphanidermatum. On the basis of mass spectrometry (Q-ToF MS), we determined that Artepillin C, the major constituent of green propolis, underlies the effect and displays activity against P. aphanidermatum at a minimal inhibitory concentration of 750 µg.mL-1. Biophysical studies based on model membranes showed that this inhibitory effect may be linked with a membrane-related phenomenon: Artepillin C increases the permeability of membranes with relatively high fluidity in their lateral structure, a feature that is in line with the lipid composition reported for the cytoplasmic membrane of P. aphanidermatum. Therefore, the present study supports the use of the effective and inexpensive green propolis to control the impact of the dangerous phytopathogen P. aphanidermatum on agriculture.
publishDate 2019
dc.date.none.fl_str_mv 2019-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/128584
Moreira Pazin, Wallance; Santos, Suikinai N. Dos; Queiroz, Sonia C. N.; Bagatolli, Luis Alberto; Soares, Ademilson E. E.; et al.; Bioactivity and action mechanism of green propolis against Pythium aphanidermatum; Academia Brasileira de Ciencias; Anais da Academia Brasileira de Ciencias; 91; 2; 7-2019; 1-9
1678-2690
CONICET Digital
CONICET
url http://hdl.handle.net/11336/128584
identifier_str_mv Moreira Pazin, Wallance; Santos, Suikinai N. Dos; Queiroz, Sonia C. N.; Bagatolli, Luis Alberto; Soares, Ademilson E. E.; et al.; Bioactivity and action mechanism of green propolis against Pythium aphanidermatum; Academia Brasileira de Ciencias; Anais da Academia Brasileira de Ciencias; 91; 2; 7-2019; 1-9
1678-2690
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1590/0001-3765201920180598
info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/w5m3sk
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academia Brasileira de Ciencias
publisher.none.fl_str_mv Academia Brasileira de Ciencias
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613562488586240
score 13.070432

Publicaciones similares