The iodide-transport-defect-causing mutation R124H: a δ-amino group at position 124 is critical for maturation and trafficking of the Na+/I− symporter

• Autores: Paroder, Viktoriya; Nicola, Juan Pablo; Ginter, Christopher S.; Carrasco, Nancy
• Año de publicación: 2013
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: Na+/I- SYMPORTER; IODIDE TRANSPORT DEFECT; HOMOLOGY MODEL; PLASMA MEMBRANE TRAFFICKING
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

Na+ /I2 symporter (NIS)-mediated active accumulation of I2 in thyrocytes is a key step in the biosynthesis of the iodine-containing thyroid hormones T3 and T4. Several NIS mutants have been identified as a cause of congenital I2 transport defect (ITD), and their investigation has yielded valuable mechanistic information on NIS. Here we report novel findings derived from the thorough characterization of the ITD-causing mutation R124H, located in the second intracellular loop (IL-2). R124H NIS is incompletely glycosylated and colocalizes with endoplasmic reticulum (ER)-resident protein markers. As a result, R124H NIS is not targeted to the plasma membrane and therefore does not mediate any I2 transport in transfected COS-7 cells. Strikingly, however, the mutant is intrinsically active, as revealed by its ability to mediate I2 transport in membrane vesicles. Of all the amino acid substitutions we carried out at position 124 (K, D, E, A, W, N and Q), only Gln restored targeting of NIS to the plasma membrane and NIS activity, suggesting a key structural role for the d-amino group of R124 in the transporter’s maturation and cell surface targeting. Using our NIS homology model based on the structure of the Vibrio parahaemolyticus Na+ /galactose symporter, we propose an interaction between the d-amino group of either R or Q124 and the thiol group of C440, located in IL-6. We conclude that the interaction between IL-2 and IL-6 is critical for the local folding required for NIS maturation and plasma membrane trafficking.
Fil: Paroder, Viktoriya. Albert Einstein College of Medicine; Estados Unidos
Fil: Nicola, Juan Pablo. University of Yale; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ginter, Christopher S.. Albert Einstein College of Medicine; Estados Unidos
Fil: Carrasco, Nancy. Albert Einstein College of Medicine; Estados Unidos. University of Yale; Estados Unidos