Allicin neuroprotective effect during oxidative/inflammatory injury involves at1-hsp70-inos counterbalance axis

Autores
Mazzei, Luciana Jorgelina; Ruiz Roso, María Belén; de Las Heras, Natalia; Ballesteros, Sandra; Torres Palazzolo, Carolina Andrea; León Ferder; Camargo, Alejandra Beatriz; Manucha, Walter Ariel Fernando
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The ancestral cultures have described many therapeutic properties of garlic; therefore, it is of central interest to elucidate the molecular basis explaining this millenary empirical knowledge. Indeed, it has been demonstrated a neuroprotective effect of allicin–a phytochemical present in garlic- linked to oxidative-inflammatory modulation. Allicin improved neuronal injury by heat shock protein 70 (Hsp70) and inducible nitric oxide synthase (iNOS) regulation. Also, allicin exerts renal protection involving a possible angiotensin type 1 receptor (AT1) interaction. In connection, AT1 overexpression has been recognized as a central deleterious factor in many brain diseases. However, there are no studies that evaluate AT1-Hsp70-iNOS interaction as a mechanism linked to neuroinflammation. Thus, our central aim is to evaluate if the allicin protective effect is associated with an AT1-Hsp70-iNOS counterbalance axis. For this study, a murine microglial cell line (BV-2) was injured with lipopolysaccharides and treated or not with allicin. Then, it was evaluated cell viability, proinflammatory cytokine levels, cellular oxidative stress, iNOS, Hsp70, and AT1 protein expression (cellular and mitochondrial fractions), nitrite levels, and protein-protein interactions. The results demonstrated that allicin could prevent neuronal injury due to a reduction in oxidative stress and inflammatory status mediated by an AT1-Hsp70-iNOS counterbalance axis linked to direct protein-protein interaction.
Fil: Mazzei, Luciana Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Ruiz Roso, María Belén. Universidad Complutense de Madrid. Facultad de Medicina. Departamento de Fisiología; España
Fil: de Las Heras, Natalia. Universidad Complutense de Madrid. Facultad de Medicina. Departamento de Fisiología; España
Fil: Ballesteros, Sandra. Universidad Complutense de Madrid. Facultad de Medicina. Departamento de Fisiología; España
Fil: Torres Palazzolo, Carolina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: León Ferder. University of Miami; Estados Unidos
Fil: Camargo, Alejandra Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Manucha, Walter Ariel Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Materia
ALLICIN
AT1 RECEPTORS
BV-2 CELLS
HSP70
INOS
NEUROINFLAMMATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/142124
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/142124
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Allicin neuroprotective effect during oxidative/inflammatory injury involves at1-hsp70-inos counterbalance axisMazzei, Luciana JorgelinaRuiz Roso, María Belénde Las Heras, NataliaBallesteros, SandraTorres Palazzolo, Carolina AndreaLeón FerderCamargo, Alejandra BeatrizManucha, Walter Ariel FernandoALLICINAT1 RECEPTORSBV-2 CELLSHSP70INOSNEUROINFLAMMATIONhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The ancestral cultures have described many therapeutic properties of garlic; therefore, it is of central interest to elucidate the molecular basis explaining this millenary empirical knowledge. Indeed, it has been demonstrated a neuroprotective effect of allicin–a phytochemical present in garlic- linked to oxidative-inflammatory modulation. Allicin improved neuronal injury by heat shock protein 70 (Hsp70) and inducible nitric oxide synthase (iNOS) regulation. Also, allicin exerts renal protection involving a possible angiotensin type 1 receptor (AT1) interaction. In connection, AT1 overexpression has been recognized as a central deleterious factor in many brain diseases. However, there are no studies that evaluate AT1-Hsp70-iNOS interaction as a mechanism linked to neuroinflammation. Thus, our central aim is to evaluate if the allicin protective effect is associated with an AT1-Hsp70-iNOS counterbalance axis. For this study, a murine microglial cell line (BV-2) was injured with lipopolysaccharides and treated or not with allicin. Then, it was evaluated cell viability, proinflammatory cytokine levels, cellular oxidative stress, iNOS, Hsp70, and AT1 protein expression (cellular and mitochondrial fractions), nitrite levels, and protein-protein interactions. The results demonstrated that allicin could prevent neuronal injury due to a reduction in oxidative stress and inflammatory status mediated by an AT1-Hsp70-iNOS counterbalance axis linked to direct protein-protein interaction.Fil: Mazzei, Luciana Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaFil: Ruiz Roso, María Belén. Universidad Complutense de Madrid. Facultad de Medicina. Departamento de Fisiología; EspañaFil: de Las Heras, Natalia. Universidad Complutense de Madrid. Facultad de Medicina. Departamento de Fisiología; EspañaFil: Ballesteros, Sandra. Universidad Complutense de Madrid. Facultad de Medicina. Departamento de Fisiología; EspañaFil: Torres Palazzolo, Carolina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: León Ferder. University of Miami; Estados UnidosFil: Camargo, Alejandra Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Manucha, Walter Ariel Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaInstituto de Histología y Embriología2020-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/142124Mazzei, Luciana Jorgelina; Ruiz Roso, María Belén; de Las Heras, Natalia; Ballesteros, Sandra; Torres Palazzolo, Carolina Andrea; et al.; Allicin neuroprotective effect during oxidative/inflammatory injury involves at1-hsp70-inos counterbalance axis; Instituto de Histología y Embriología; Biocell; 44; 4; 12-2020; 671-6811667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.32604/biocell.2020.014175info:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v44n4/40983info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:00:15Zoai:ri.conicet.gov.ar:11336/142124instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:00:15.751CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Allicin neuroprotective effect during oxidative/inflammatory injury involves at1-hsp70-inos counterbalance axis
title Allicin neuroprotective effect during oxidative/inflammatory injury involves at1-hsp70-inos counterbalance axis
spellingShingle Allicin neuroprotective effect during oxidative/inflammatory injury involves at1-hsp70-inos counterbalance axis
Mazzei, Luciana Jorgelina
ALLICIN
AT1 RECEPTORS
BV-2 CELLS
HSP70
INOS
NEUROINFLAMMATION
title_short Allicin neuroprotective effect during oxidative/inflammatory injury involves at1-hsp70-inos counterbalance axis
title_full Allicin neuroprotective effect during oxidative/inflammatory injury involves at1-hsp70-inos counterbalance axis
title_fullStr Allicin neuroprotective effect during oxidative/inflammatory injury involves at1-hsp70-inos counterbalance axis
title_full_unstemmed Allicin neuroprotective effect during oxidative/inflammatory injury involves at1-hsp70-inos counterbalance axis
title_sort Allicin neuroprotective effect during oxidative/inflammatory injury involves at1-hsp70-inos counterbalance axis
dc.creator.none.fl_str_mv Mazzei, Luciana Jorgelina
Ruiz Roso, María Belén
de Las Heras, Natalia
Ballesteros, Sandra
Torres Palazzolo, Carolina Andrea
León Ferder
Camargo, Alejandra Beatriz
Manucha, Walter Ariel Fernando
author Mazzei, Luciana Jorgelina
author_facet Mazzei, Luciana Jorgelina
Ruiz Roso, María Belén
de Las Heras, Natalia
Ballesteros, Sandra
Torres Palazzolo, Carolina Andrea
León Ferder
Camargo, Alejandra Beatriz
Manucha, Walter Ariel Fernando
author_role author
author2 Ruiz Roso, María Belén
de Las Heras, Natalia
Ballesteros, Sandra
Torres Palazzolo, Carolina Andrea
León Ferder
Camargo, Alejandra Beatriz
Manucha, Walter Ariel Fernando
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ALLICIN
AT1 RECEPTORS
BV-2 CELLS
HSP70
INOS
NEUROINFLAMMATION
topic ALLICIN
AT1 RECEPTORS
BV-2 CELLS
HSP70
INOS
NEUROINFLAMMATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The ancestral cultures have described many therapeutic properties of garlic; therefore, it is of central interest to elucidate the molecular basis explaining this millenary empirical knowledge. Indeed, it has been demonstrated a neuroprotective effect of allicin–a phytochemical present in garlic- linked to oxidative-inflammatory modulation. Allicin improved neuronal injury by heat shock protein 70 (Hsp70) and inducible nitric oxide synthase (iNOS) regulation. Also, allicin exerts renal protection involving a possible angiotensin type 1 receptor (AT1) interaction. In connection, AT1 overexpression has been recognized as a central deleterious factor in many brain diseases. However, there are no studies that evaluate AT1-Hsp70-iNOS interaction as a mechanism linked to neuroinflammation. Thus, our central aim is to evaluate if the allicin protective effect is associated with an AT1-Hsp70-iNOS counterbalance axis. For this study, a murine microglial cell line (BV-2) was injured with lipopolysaccharides and treated or not with allicin. Then, it was evaluated cell viability, proinflammatory cytokine levels, cellular oxidative stress, iNOS, Hsp70, and AT1 protein expression (cellular and mitochondrial fractions), nitrite levels, and protein-protein interactions. The results demonstrated that allicin could prevent neuronal injury due to a reduction in oxidative stress and inflammatory status mediated by an AT1-Hsp70-iNOS counterbalance axis linked to direct protein-protein interaction.
Fil: Mazzei, Luciana Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Ruiz Roso, María Belén. Universidad Complutense de Madrid. Facultad de Medicina. Departamento de Fisiología; España
Fil: de Las Heras, Natalia. Universidad Complutense de Madrid. Facultad de Medicina. Departamento de Fisiología; España
Fil: Ballesteros, Sandra. Universidad Complutense de Madrid. Facultad de Medicina. Departamento de Fisiología; España
Fil: Torres Palazzolo, Carolina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: León Ferder. University of Miami; Estados Unidos
Fil: Camargo, Alejandra Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Manucha, Walter Ariel Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
description The ancestral cultures have described many therapeutic properties of garlic; therefore, it is of central interest to elucidate the molecular basis explaining this millenary empirical knowledge. Indeed, it has been demonstrated a neuroprotective effect of allicin–a phytochemical present in garlic- linked to oxidative-inflammatory modulation. Allicin improved neuronal injury by heat shock protein 70 (Hsp70) and inducible nitric oxide synthase (iNOS) regulation. Also, allicin exerts renal protection involving a possible angiotensin type 1 receptor (AT1) interaction. In connection, AT1 overexpression has been recognized as a central deleterious factor in many brain diseases. However, there are no studies that evaluate AT1-Hsp70-iNOS interaction as a mechanism linked to neuroinflammation. Thus, our central aim is to evaluate if the allicin protective effect is associated with an AT1-Hsp70-iNOS counterbalance axis. For this study, a murine microglial cell line (BV-2) was injured with lipopolysaccharides and treated or not with allicin. Then, it was evaluated cell viability, proinflammatory cytokine levels, cellular oxidative stress, iNOS, Hsp70, and AT1 protein expression (cellular and mitochondrial fractions), nitrite levels, and protein-protein interactions. The results demonstrated that allicin could prevent neuronal injury due to a reduction in oxidative stress and inflammatory status mediated by an AT1-Hsp70-iNOS counterbalance axis linked to direct protein-protein interaction.
publishDate 2020
dc.date.none.fl_str_mv 2020-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/142124
Mazzei, Luciana Jorgelina; Ruiz Roso, María Belén; de Las Heras, Natalia; Ballesteros, Sandra; Torres Palazzolo, Carolina Andrea; et al.; Allicin neuroprotective effect during oxidative/inflammatory injury involves at1-hsp70-inos counterbalance axis; Instituto de Histología y Embriología; Biocell; 44; 4; 12-2020; 671-681
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/142124
identifier_str_mv Mazzei, Luciana Jorgelina; Ruiz Roso, María Belén; de Las Heras, Natalia; Ballesteros, Sandra; Torres Palazzolo, Carolina Andrea; et al.; Allicin neuroprotective effect during oxidative/inflammatory injury involves at1-hsp70-inos counterbalance axis; Instituto de Histología y Embriología; Biocell; 44; 4; 12-2020; 671-681
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.32604/biocell.2020.014175
info:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v44n4/40983
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Instituto de Histología y Embriología
publisher.none.fl_str_mv Instituto de Histología y Embriología
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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