Differential infectivity of two Trypanosoma cruzi strains in placental cells and tissue

Autores
Medina, Lisvaneth; Castillo, Christian; Liempi, Ana; Herbach, Mathias; Cabrera, Gonzalo; Valenzuela, Lucía; Galanti, Norbel; Curto, Maria de Los Angeles; Schijman, Alejandro Gabriel; Kemmerling, Ulrike
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Congenital Chagas disease, caused by Trypanosoma cruzi (T. cruzi), has become epidemiologically relevant. The probability of congenital transmission depends on the maternal and developing fetal/newborn immune responses, placental factors and importantly, the virulence of the parasite. It has been proposed, that different genotypes of T. cruzi and their associated pathogenicity, virulence and tissue tropism may play an important role in congenital infection. Since there is no laboratory or animal model that recapitulates the complexities of vertical transmission in humans, here we studied parasite infectivity in human placental explants (HPE) as well as in the human trophoblast-derived cell line BeWo of the Y(DTU II) and the VD (TcVI) T. cruzi strains; the latter was isolated from a human case of congenital infection. Our results show that the VD strain is more infective and pathogenic than the Y strain, as demonstrated by qPCR and cell counting as well as by histopathological analysis. The present study constitutes the first approach to study the relationship between parasite two parasite strains from different genotypes and the infection efficiency in human placenta.
Fil: Medina, Lisvaneth. Universidad de Chile; Chile
Fil: Castillo, Christian. Universidad de Chile; Chile
Fil: Liempi, Ana. Universidad de Chile; Chile
Fil: Herbach, Mathias. Universidad de Chile; Chile
Fil: Cabrera, Gonzalo. Universidad de Chile; Chile
Fil: Valenzuela, Lucía. Universidad de Chile; Chile
Fil: Galanti, Norbel. Universidad de Chile; Chile
Fil: Curto, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Kemmerling, Ulrike. Universidad de Chile; Chile
Materia
Genotypes
Human Placenta
Tropism
Trypanosoma Cruzi
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/79831
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/79831
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Differential infectivity of two Trypanosoma cruzi strains in placental cells and tissueMedina, LisvanethCastillo, ChristianLiempi, AnaHerbach, MathiasCabrera, GonzaloValenzuela, LucíaGalanti, NorbelCurto, Maria de Los AngelesSchijman, Alejandro GabrielKemmerling, UlrikeGenotypesHuman PlacentaTropismTrypanosoma Cruzihttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Congenital Chagas disease, caused by Trypanosoma cruzi (T. cruzi), has become epidemiologically relevant. The probability of congenital transmission depends on the maternal and developing fetal/newborn immune responses, placental factors and importantly, the virulence of the parasite. It has been proposed, that different genotypes of T. cruzi and their associated pathogenicity, virulence and tissue tropism may play an important role in congenital infection. Since there is no laboratory or animal model that recapitulates the complexities of vertical transmission in humans, here we studied parasite infectivity in human placental explants (HPE) as well as in the human trophoblast-derived cell line BeWo of the Y(DTU II) and the VD (TcVI) T. cruzi strains; the latter was isolated from a human case of congenital infection. Our results show that the VD strain is more infective and pathogenic than the Y strain, as demonstrated by qPCR and cell counting as well as by histopathological analysis. The present study constitutes the first approach to study the relationship between parasite two parasite strains from different genotypes and the infection efficiency in human placenta.Fil: Medina, Lisvaneth. Universidad de Chile; ChileFil: Castillo, Christian. Universidad de Chile; ChileFil: Liempi, Ana. Universidad de Chile; ChileFil: Herbach, Mathias. Universidad de Chile; ChileFil: Cabrera, Gonzalo. Universidad de Chile; ChileFil: Valenzuela, Lucía. Universidad de Chile; ChileFil: Galanti, Norbel. Universidad de Chile; ChileFil: Curto, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Kemmerling, Ulrike. Universidad de Chile; ChileElsevier Science2018-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/79831Medina, Lisvaneth; Castillo, Christian; Liempi, Ana; Herbach, Mathias; Cabrera, Gonzalo; et al.; Differential infectivity of two Trypanosoma cruzi strains in placental cells and tissue; Elsevier Science; Acta Tropica; 186; 10-2018; 35-400001-706XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.actatropica.2018.07.001info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/30018029info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0001706X18300214info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T10:46:45Zoai:ri.conicet.gov.ar:11336/79831instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 10:46:45.689CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Differential infectivity of two Trypanosoma cruzi strains in placental cells and tissue
title Differential infectivity of two Trypanosoma cruzi strains in placental cells and tissue
spellingShingle Differential infectivity of two Trypanosoma cruzi strains in placental cells and tissue
Medina, Lisvaneth
Genotypes
Human Placenta
Tropism
Trypanosoma Cruzi
title_short Differential infectivity of two Trypanosoma cruzi strains in placental cells and tissue
title_full Differential infectivity of two Trypanosoma cruzi strains in placental cells and tissue
title_fullStr Differential infectivity of two Trypanosoma cruzi strains in placental cells and tissue
title_full_unstemmed Differential infectivity of two Trypanosoma cruzi strains in placental cells and tissue
title_sort Differential infectivity of two Trypanosoma cruzi strains in placental cells and tissue
dc.creator.none.fl_str_mv Medina, Lisvaneth
Castillo, Christian
Liempi, Ana
Herbach, Mathias
Cabrera, Gonzalo
Valenzuela, Lucía
Galanti, Norbel
Curto, Maria de Los Angeles
Schijman, Alejandro Gabriel
Kemmerling, Ulrike
author Medina, Lisvaneth
author_facet Medina, Lisvaneth
Castillo, Christian
Liempi, Ana
Herbach, Mathias
Cabrera, Gonzalo
Valenzuela, Lucía
Galanti, Norbel
Curto, Maria de Los Angeles
Schijman, Alejandro Gabriel
Kemmerling, Ulrike
author_role author
author2 Castillo, Christian
Liempi, Ana
Herbach, Mathias
Cabrera, Gonzalo
Valenzuela, Lucía
Galanti, Norbel
Curto, Maria de Los Angeles
Schijman, Alejandro Gabriel
Kemmerling, Ulrike
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Genotypes
Human Placenta
Tropism
Trypanosoma Cruzi
topic Genotypes
Human Placenta
Tropism
Trypanosoma Cruzi
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Congenital Chagas disease, caused by Trypanosoma cruzi (T. cruzi), has become epidemiologically relevant. The probability of congenital transmission depends on the maternal and developing fetal/newborn immune responses, placental factors and importantly, the virulence of the parasite. It has been proposed, that different genotypes of T. cruzi and their associated pathogenicity, virulence and tissue tropism may play an important role in congenital infection. Since there is no laboratory or animal model that recapitulates the complexities of vertical transmission in humans, here we studied parasite infectivity in human placental explants (HPE) as well as in the human trophoblast-derived cell line BeWo of the Y(DTU II) and the VD (TcVI) T. cruzi strains; the latter was isolated from a human case of congenital infection. Our results show that the VD strain is more infective and pathogenic than the Y strain, as demonstrated by qPCR and cell counting as well as by histopathological analysis. The present study constitutes the first approach to study the relationship between parasite two parasite strains from different genotypes and the infection efficiency in human placenta.
Fil: Medina, Lisvaneth. Universidad de Chile; Chile
Fil: Castillo, Christian. Universidad de Chile; Chile
Fil: Liempi, Ana. Universidad de Chile; Chile
Fil: Herbach, Mathias. Universidad de Chile; Chile
Fil: Cabrera, Gonzalo. Universidad de Chile; Chile
Fil: Valenzuela, Lucía. Universidad de Chile; Chile
Fil: Galanti, Norbel. Universidad de Chile; Chile
Fil: Curto, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Kemmerling, Ulrike. Universidad de Chile; Chile
description Congenital Chagas disease, caused by Trypanosoma cruzi (T. cruzi), has become epidemiologically relevant. The probability of congenital transmission depends on the maternal and developing fetal/newborn immune responses, placental factors and importantly, the virulence of the parasite. It has been proposed, that different genotypes of T. cruzi and their associated pathogenicity, virulence and tissue tropism may play an important role in congenital infection. Since there is no laboratory or animal model that recapitulates the complexities of vertical transmission in humans, here we studied parasite infectivity in human placental explants (HPE) as well as in the human trophoblast-derived cell line BeWo of the Y(DTU II) and the VD (TcVI) T. cruzi strains; the latter was isolated from a human case of congenital infection. Our results show that the VD strain is more infective and pathogenic than the Y strain, as demonstrated by qPCR and cell counting as well as by histopathological analysis. The present study constitutes the first approach to study the relationship between parasite two parasite strains from different genotypes and the infection efficiency in human placenta.
publishDate 2018
dc.date.none.fl_str_mv 2018-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/79831
Medina, Lisvaneth; Castillo, Christian; Liempi, Ana; Herbach, Mathias; Cabrera, Gonzalo; et al.; Differential infectivity of two Trypanosoma cruzi strains in placental cells and tissue; Elsevier Science; Acta Tropica; 186; 10-2018; 35-40
0001-706X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/79831
identifier_str_mv Medina, Lisvaneth; Castillo, Christian; Liempi, Ana; Herbach, Mathias; Cabrera, Gonzalo; et al.; Differential infectivity of two Trypanosoma cruzi strains in placental cells and tissue; Elsevier Science; Acta Tropica; 186; 10-2018; 35-40
0001-706X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.actatropica.2018.07.001
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/30018029
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0001706X18300214
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.001348

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