Quantification of human infection risk caused by rotavirus in surface waters from Córdoba, Argentina

Autores
Prez, Verónica Emilse; Gil, Pedro Ignacio; Temprana, Carlos Facundo; Cuadrado, P. R.; Martínez, L.C.; Giordano, Miguel Oscar; Masachessi, Gisela; Isa, Maria Beatriz; Ré, Viviana Elizabeth; Pavan, Jorge Victorio; Nates, Silvia Viviana; Barril, Patricia Angelica
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fecal contamination of water is a worrying problem because it is associated with the transmission of enteric pathogenic microorganisms that can cause many infectious diseases. In this study, an environmental survey was conducted to assess the level of viral contamination by viable enterovirus and rotavirus genome in two recreational rivers (Suquía and Xanaes) of Córdoba, Argentina. Quantitative microbial risk assessment (QMRA) was calculated to estimate the risk of rotavirus infection. Water sampling was carried out during a one-year period, the presence of total and fecal coliforms was determined and water samples were then concentrated for viral determination. Cell culture and indirect immunofluorescence were applied for enterovirus detection and RT-qPCR for rotavirus quantification. Coliform bacteria levels found in Suquía River often far exceeded the guideline limits for recreational waters. The Xanaes exhibited a lower level of bacterial contamination, frequently within the guideline limits. Enterovirus and rotavirus were frequently detected in the monitoring rivers (percentage of positive samples in Suquía: 78.6% enterovirus, 100% rotavirus; in Xanaes: 87.5% enterovirus, 18.7% rotavirus). Rotavirus was detected at a media concentration of 5.7 × 105 genome copies/L (gc/L) in the Suquía and 8.5 × 100 gc/L in the Xanaes. QMRA revealed high risk of rotavirus infection in the Suquía, at sampling points with acceptable and non-acceptable bacteria numbers. The Xanaes showed significantly lower health risk of rotavirus infection but it proved to be a public health hazard. The viral occurrence was not readily explained by the levels of bacteria indicators, thus viral monitoring should be included to determine microbiological water quality. These findings provide the first data of QMRA for recreational waters in Argentina and reveal the need for public awareness of the health implications of the use of the river waters.
Fil: Prez, Verónica Emilse. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Gil, Pedro Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Temprana, Carlos Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina
Fil: Cuadrado, P. R.. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Martínez, L.C.. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Giordano, Miguel Oscar. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Masachessi, Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Isa, Maria Beatriz. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Ré, Viviana Elizabeth. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Pavan, Jorge Victorio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Nates, Silvia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Barril, Patricia Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Materia
Environmental Surveillance
Enterovirus
Rotavirus
Water Quality
Surface Water
Quantitative Microbial Risk Assessment (Qmra)
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/43420
Acceder
id CONICETDig_1d7ff400137ece96ca702656c59cd7d9
oai_identifier_str oai:ri.conicet.gov.ar:11336/43420
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Quantification of human infection risk caused by rotavirus in surface waters from Córdoba, ArgentinaPrez, Verónica EmilseGil, Pedro IgnacioTemprana, Carlos FacundoCuadrado, P. R.Martínez, L.C.Giordano, Miguel OscarMasachessi, GiselaIsa, Maria BeatrizRé, Viviana ElizabethPavan, Jorge VictorioNates, Silvia VivianaBarril, Patricia AngelicaEnvironmental SurveillanceEnterovirusRotavirusWater QualitySurface WaterQuantitative Microbial Risk Assessment (Qmra)https://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Fecal contamination of water is a worrying problem because it is associated with the transmission of enteric pathogenic microorganisms that can cause many infectious diseases. In this study, an environmental survey was conducted to assess the level of viral contamination by viable enterovirus and rotavirus genome in two recreational rivers (Suquía and Xanaes) of Córdoba, Argentina. Quantitative microbial risk assessment (QMRA) was calculated to estimate the risk of rotavirus infection. Water sampling was carried out during a one-year period, the presence of total and fecal coliforms was determined and water samples were then concentrated for viral determination. Cell culture and indirect immunofluorescence were applied for enterovirus detection and RT-qPCR for rotavirus quantification. Coliform bacteria levels found in Suquía River often far exceeded the guideline limits for recreational waters. The Xanaes exhibited a lower level of bacterial contamination, frequently within the guideline limits. Enterovirus and rotavirus were frequently detected in the monitoring rivers (percentage of positive samples in Suquía: 78.6% enterovirus, 100% rotavirus; in Xanaes: 87.5% enterovirus, 18.7% rotavirus). Rotavirus was detected at a media concentration of 5.7 × 105 genome copies/L (gc/L) in the Suquía and 8.5 × 100 gc/L in the Xanaes. QMRA revealed high risk of rotavirus infection in the Suquía, at sampling points with acceptable and non-acceptable bacteria numbers. The Xanaes showed significantly lower health risk of rotavirus infection but it proved to be a public health hazard. The viral occurrence was not readily explained by the levels of bacteria indicators, thus viral monitoring should be included to determine microbiological water quality. These findings provide the first data of QMRA for recreational waters in Argentina and reveal the need for public awareness of the health implications of the use of the river waters.Fil: Prez, Verónica Emilse. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; ArgentinaFil: Gil, Pedro Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; ArgentinaFil: Temprana, Carlos Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; ArgentinaFil: Cuadrado, P. R.. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; ArgentinaFil: Martínez, L.C.. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; ArgentinaFil: Giordano, Miguel Oscar. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; ArgentinaFil: Masachessi, Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; ArgentinaFil: Isa, Maria Beatriz. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; ArgentinaFil: Ré, Viviana Elizabeth. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; ArgentinaFil: Pavan, Jorge Victorio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; ArgentinaFil: Nates, Silvia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; ArgentinaFil: Barril, Patricia Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; ArgentinaElsevier Science2015-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/43420Prez, Verónica Emilse; Gil, Pedro Ignacio; Temprana, Carlos Facundo; Cuadrado, P. R.; Martínez, L.C.; et al.; Quantification of human infection risk caused by rotavirus in surface waters from Córdoba, Argentina; Elsevier Science; The Science Of Total Environment; 538; 12-2015; 220-2290048-9697CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.scitotenv.2015.08.041info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S004896971530543Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:48:33Zoai:ri.conicet.gov.ar:11336/43420instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:48:33.343CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Quantification of human infection risk caused by rotavirus in surface waters from Córdoba, Argentina
title Quantification of human infection risk caused by rotavirus in surface waters from Córdoba, Argentina
spellingShingle Quantification of human infection risk caused by rotavirus in surface waters from Córdoba, Argentina
Prez, Verónica Emilse
Environmental Surveillance
Enterovirus
Rotavirus
Water Quality
Surface Water
Quantitative Microbial Risk Assessment (Qmra)
title_short Quantification of human infection risk caused by rotavirus in surface waters from Córdoba, Argentina
title_full Quantification of human infection risk caused by rotavirus in surface waters from Córdoba, Argentina
title_fullStr Quantification of human infection risk caused by rotavirus in surface waters from Córdoba, Argentina
title_full_unstemmed Quantification of human infection risk caused by rotavirus in surface waters from Córdoba, Argentina
title_sort Quantification of human infection risk caused by rotavirus in surface waters from Córdoba, Argentina
dc.creator.none.fl_str_mv Prez, Verónica Emilse
Gil, Pedro Ignacio
Temprana, Carlos Facundo
Cuadrado, P. R.
Martínez, L.C.
Giordano, Miguel Oscar
Masachessi, Gisela
Isa, Maria Beatriz
Ré, Viviana Elizabeth
Pavan, Jorge Victorio
Nates, Silvia Viviana
Barril, Patricia Angelica
author Prez, Verónica Emilse
author_facet Prez, Verónica Emilse
Gil, Pedro Ignacio
Temprana, Carlos Facundo
Cuadrado, P. R.
Martínez, L.C.
Giordano, Miguel Oscar
Masachessi, Gisela
Isa, Maria Beatriz
Ré, Viviana Elizabeth
Pavan, Jorge Victorio
Nates, Silvia Viviana
Barril, Patricia Angelica
author_role author
author2 Gil, Pedro Ignacio
Temprana, Carlos Facundo
Cuadrado, P. R.
Martínez, L.C.
Giordano, Miguel Oscar
Masachessi, Gisela
Isa, Maria Beatriz
Ré, Viviana Elizabeth
Pavan, Jorge Victorio
Nates, Silvia Viviana
Barril, Patricia Angelica
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Environmental Surveillance
Enterovirus
Rotavirus
Water Quality
Surface Water
Quantitative Microbial Risk Assessment (Qmra)
topic Environmental Surveillance
Enterovirus
Rotavirus
Water Quality
Surface Water
Quantitative Microbial Risk Assessment (Qmra)
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Fecal contamination of water is a worrying problem because it is associated with the transmission of enteric pathogenic microorganisms that can cause many infectious diseases. In this study, an environmental survey was conducted to assess the level of viral contamination by viable enterovirus and rotavirus genome in two recreational rivers (Suquía and Xanaes) of Córdoba, Argentina. Quantitative microbial risk assessment (QMRA) was calculated to estimate the risk of rotavirus infection. Water sampling was carried out during a one-year period, the presence of total and fecal coliforms was determined and water samples were then concentrated for viral determination. Cell culture and indirect immunofluorescence were applied for enterovirus detection and RT-qPCR for rotavirus quantification. Coliform bacteria levels found in Suquía River often far exceeded the guideline limits for recreational waters. The Xanaes exhibited a lower level of bacterial contamination, frequently within the guideline limits. Enterovirus and rotavirus were frequently detected in the monitoring rivers (percentage of positive samples in Suquía: 78.6% enterovirus, 100% rotavirus; in Xanaes: 87.5% enterovirus, 18.7% rotavirus). Rotavirus was detected at a media concentration of 5.7 × 105 genome copies/L (gc/L) in the Suquía and 8.5 × 100 gc/L in the Xanaes. QMRA revealed high risk of rotavirus infection in the Suquía, at sampling points with acceptable and non-acceptable bacteria numbers. The Xanaes showed significantly lower health risk of rotavirus infection but it proved to be a public health hazard. The viral occurrence was not readily explained by the levels of bacteria indicators, thus viral monitoring should be included to determine microbiological water quality. These findings provide the first data of QMRA for recreational waters in Argentina and reveal the need for public awareness of the health implications of the use of the river waters.
Fil: Prez, Verónica Emilse. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Gil, Pedro Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Temprana, Carlos Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina
Fil: Cuadrado, P. R.. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Martínez, L.C.. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Giordano, Miguel Oscar. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Masachessi, Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Isa, Maria Beatriz. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Ré, Viviana Elizabeth. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Pavan, Jorge Victorio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Nates, Silvia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Barril, Patricia Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
description Fecal contamination of water is a worrying problem because it is associated with the transmission of enteric pathogenic microorganisms that can cause many infectious diseases. In this study, an environmental survey was conducted to assess the level of viral contamination by viable enterovirus and rotavirus genome in two recreational rivers (Suquía and Xanaes) of Córdoba, Argentina. Quantitative microbial risk assessment (QMRA) was calculated to estimate the risk of rotavirus infection. Water sampling was carried out during a one-year period, the presence of total and fecal coliforms was determined and water samples were then concentrated for viral determination. Cell culture and indirect immunofluorescence were applied for enterovirus detection and RT-qPCR for rotavirus quantification. Coliform bacteria levels found in Suquía River often far exceeded the guideline limits for recreational waters. The Xanaes exhibited a lower level of bacterial contamination, frequently within the guideline limits. Enterovirus and rotavirus were frequently detected in the monitoring rivers (percentage of positive samples in Suquía: 78.6% enterovirus, 100% rotavirus; in Xanaes: 87.5% enterovirus, 18.7% rotavirus). Rotavirus was detected at a media concentration of 5.7 × 105 genome copies/L (gc/L) in the Suquía and 8.5 × 100 gc/L in the Xanaes. QMRA revealed high risk of rotavirus infection in the Suquía, at sampling points with acceptable and non-acceptable bacteria numbers. The Xanaes showed significantly lower health risk of rotavirus infection but it proved to be a public health hazard. The viral occurrence was not readily explained by the levels of bacteria indicators, thus viral monitoring should be included to determine microbiological water quality. These findings provide the first data of QMRA for recreational waters in Argentina and reveal the need for public awareness of the health implications of the use of the river waters.
publishDate 2015
dc.date.none.fl_str_mv 2015-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/43420
Prez, Verónica Emilse; Gil, Pedro Ignacio; Temprana, Carlos Facundo; Cuadrado, P. R.; Martínez, L.C.; et al.; Quantification of human infection risk caused by rotavirus in surface waters from Córdoba, Argentina; Elsevier Science; The Science Of Total Environment; 538; 12-2015; 220-229
0048-9697
CONICET Digital
CONICET
url http://hdl.handle.net/11336/43420
identifier_str_mv Prez, Verónica Emilse; Gil, Pedro Ignacio; Temprana, Carlos Facundo; Cuadrado, P. R.; Martínez, L.C.; et al.; Quantification of human infection risk caused by rotavirus in surface waters from Córdoba, Argentina; Elsevier Science; The Science Of Total Environment; 538; 12-2015; 220-229
0048-9697
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.scitotenv.2015.08.041
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S004896971530543X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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application/pdf
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dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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