Intranasal administration of TLR agonists induces a discriminated local innate response along murine respiratory tract

Autores
Errea, Agustina Juliana; González Maciel, María Dolores; Hiriart, Yanina; Hozbor, Daniela Flavia; Rumbo, Martín
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Adjuvants are relevant for mucosal immunization in order to induce long lasting protective immunity. It has been shown that targeting to different regions of the airway results in different capacity to trigger adaptive/protective immunity. Nevertheless there is scarce knowledge regarding topological responsiveness along airways to TLR agonists. We analyzed the effects of intranasal administration of lipopolysaccharide (LPS), poly I:C and flagellin on the expression of a panel of innate response markers along murine airways by laser microdissection and RTqPCR. In all cases treatment induced recruitment of inflammatory cells to airways. However, regional gene expression indicated that whereas deeper airways (mainly alveoli) respond with high expression of IL6, CXCL1 and CXCL10, the response in conductive airways (bronchi and bronchioles) is dominated by expression of CCL20. On the other hand, triggering TLR3 elicits a response dominated by CXCL10, showing higher expression at 6 h compared to 2 h, whereas LPS and flagellin induce a response peaking at 2 h and dominated by IL6 and CXCL1. The results presented here showed difference in topological response triggered by different TLR agonist. These results make the targeting of different sites of airways a variable to evaluate when selecting the appropriate combinations of TLR and vaccinal antigens for intranasal delivery.
Fil: Errea, Agustina Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina
Fil: González Maciel, María Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina
Fil: Hiriart, Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina
Fil: Hozbor, Daniela Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Rumbo, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina
Materia
Toll Like Receptors
Tlr Agonist
Airways
Innate Response
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/45801
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Intranasal administration of TLR agonists induces a discriminated local innate response along murine respiratory tractErrea, Agustina JulianaGonzález Maciel, María DoloresHiriart, YaninaHozbor, Daniela FlaviaRumbo, MartínToll Like ReceptorsTlr AgonistAirwaysInnate Responsehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Adjuvants are relevant for mucosal immunization in order to induce long lasting protective immunity. It has been shown that targeting to different regions of the airway results in different capacity to trigger adaptive/protective immunity. Nevertheless there is scarce knowledge regarding topological responsiveness along airways to TLR agonists. We analyzed the effects of intranasal administration of lipopolysaccharide (LPS), poly I:C and flagellin on the expression of a panel of innate response markers along murine airways by laser microdissection and RTqPCR. In all cases treatment induced recruitment of inflammatory cells to airways. However, regional gene expression indicated that whereas deeper airways (mainly alveoli) respond with high expression of IL6, CXCL1 and CXCL10, the response in conductive airways (bronchi and bronchioles) is dominated by expression of CCL20. On the other hand, triggering TLR3 elicits a response dominated by CXCL10, showing higher expression at 6 h compared to 2 h, whereas LPS and flagellin induce a response peaking at 2 h and dominated by IL6 and CXCL1. The results presented here showed difference in topological response triggered by different TLR agonist. These results make the targeting of different sites of airways a variable to evaluate when selecting the appropriate combinations of TLR and vaccinal antigens for intranasal delivery.Fil: Errea, Agustina Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; ArgentinaFil: González Maciel, María Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; ArgentinaFil: Hiriart, Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; ArgentinaFil: Hozbor, Daniela Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Rumbo, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; ArgentinaElsevier Science2015-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/45801Errea, Agustina Juliana; González Maciel, María Dolores; Hiriart, Yanina; Hozbor, Daniela Flavia; Rumbo, Martín; Intranasal administration of TLR agonists induces a discriminated local innate response along murine respiratory tract; Elsevier Science; Immunology Letters; 164; 3-2015; 33-390165-2478CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.imlet.2015.01.004info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S016524781500005Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:08:38Zoai:ri.conicet.gov.ar:11336/45801instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:08:39.278CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Intranasal administration of TLR agonists induces a discriminated local innate response along murine respiratory tract
title Intranasal administration of TLR agonists induces a discriminated local innate response along murine respiratory tract
spellingShingle Intranasal administration of TLR agonists induces a discriminated local innate response along murine respiratory tract
Errea, Agustina Juliana
Toll Like Receptors
Tlr Agonist
Airways
Innate Response
title_short Intranasal administration of TLR agonists induces a discriminated local innate response along murine respiratory tract
title_full Intranasal administration of TLR agonists induces a discriminated local innate response along murine respiratory tract
title_fullStr Intranasal administration of TLR agonists induces a discriminated local innate response along murine respiratory tract
title_full_unstemmed Intranasal administration of TLR agonists induces a discriminated local innate response along murine respiratory tract
title_sort Intranasal administration of TLR agonists induces a discriminated local innate response along murine respiratory tract
dc.creator.none.fl_str_mv Errea, Agustina Juliana
González Maciel, María Dolores
Hiriart, Yanina
Hozbor, Daniela Flavia
Rumbo, Martín
author Errea, Agustina Juliana
author_facet Errea, Agustina Juliana
González Maciel, María Dolores
Hiriart, Yanina
Hozbor, Daniela Flavia
Rumbo, Martín
author_role author
author2 González Maciel, María Dolores
Hiriart, Yanina
Hozbor, Daniela Flavia
Rumbo, Martín
author2_role author
author
author
author
dc.subject.none.fl_str_mv Toll Like Receptors
Tlr Agonist
Airways
Innate Response
topic Toll Like Receptors
Tlr Agonist
Airways
Innate Response
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Adjuvants are relevant for mucosal immunization in order to induce long lasting protective immunity. It has been shown that targeting to different regions of the airway results in different capacity to trigger adaptive/protective immunity. Nevertheless there is scarce knowledge regarding topological responsiveness along airways to TLR agonists. We analyzed the effects of intranasal administration of lipopolysaccharide (LPS), poly I:C and flagellin on the expression of a panel of innate response markers along murine airways by laser microdissection and RTqPCR. In all cases treatment induced recruitment of inflammatory cells to airways. However, regional gene expression indicated that whereas deeper airways (mainly alveoli) respond with high expression of IL6, CXCL1 and CXCL10, the response in conductive airways (bronchi and bronchioles) is dominated by expression of CCL20. On the other hand, triggering TLR3 elicits a response dominated by CXCL10, showing higher expression at 6 h compared to 2 h, whereas LPS and flagellin induce a response peaking at 2 h and dominated by IL6 and CXCL1. The results presented here showed difference in topological response triggered by different TLR agonist. These results make the targeting of different sites of airways a variable to evaluate when selecting the appropriate combinations of TLR and vaccinal antigens for intranasal delivery.
Fil: Errea, Agustina Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina
Fil: González Maciel, María Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina
Fil: Hiriart, Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina
Fil: Hozbor, Daniela Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Rumbo, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina
description Adjuvants are relevant for mucosal immunization in order to induce long lasting protective immunity. It has been shown that targeting to different regions of the airway results in different capacity to trigger adaptive/protective immunity. Nevertheless there is scarce knowledge regarding topological responsiveness along airways to TLR agonists. We analyzed the effects of intranasal administration of lipopolysaccharide (LPS), poly I:C and flagellin on the expression of a panel of innate response markers along murine airways by laser microdissection and RTqPCR. In all cases treatment induced recruitment of inflammatory cells to airways. However, regional gene expression indicated that whereas deeper airways (mainly alveoli) respond with high expression of IL6, CXCL1 and CXCL10, the response in conductive airways (bronchi and bronchioles) is dominated by expression of CCL20. On the other hand, triggering TLR3 elicits a response dominated by CXCL10, showing higher expression at 6 h compared to 2 h, whereas LPS and flagellin induce a response peaking at 2 h and dominated by IL6 and CXCL1. The results presented here showed difference in topological response triggered by different TLR agonist. These results make the targeting of different sites of airways a variable to evaluate when selecting the appropriate combinations of TLR and vaccinal antigens for intranasal delivery.
publishDate 2015
dc.date.none.fl_str_mv 2015-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/45801
Errea, Agustina Juliana; González Maciel, María Dolores; Hiriart, Yanina; Hozbor, Daniela Flavia; Rumbo, Martín; Intranasal administration of TLR agonists induces a discriminated local innate response along murine respiratory tract; Elsevier Science; Immunology Letters; 164; 3-2015; 33-39
0165-2478
CONICET Digital
CONICET
url http://hdl.handle.net/11336/45801
identifier_str_mv Errea, Agustina Juliana; González Maciel, María Dolores; Hiriart, Yanina; Hozbor, Daniela Flavia; Rumbo, Martín; Intranasal administration of TLR agonists induces a discriminated local innate response along murine respiratory tract; Elsevier Science; Immunology Letters; 164; 3-2015; 33-39
0165-2478
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.imlet.2015.01.004
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S016524781500005X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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