Differential UVR8 Signal across the Stem Controls UV-B-Induced Inflorescence Phototropism

Autores
Vanhaelewyn, Lucas; Viczián, András; Prinsen, Els; Bernula, Péter; Serrano, Alejandro Miguel; Arana, Maria Veronica; Ballare, Carlos Luis; Nagy, Ferenc; Van Der Straeten, Dominique; Vandenbussche, Filip
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the course of evolution, plants have developed mechanisms that orient their organs toward the incoming light. At the seedling stage, positive phototropism is mainly regulated by phototropin photoreceptors in blue and UV wavelengths. Contrasting with this, we report that UV RESISTANCE LOCUS8 (UVR8) serves as the predominant photoreceptor of UVB–induced phototropic responses in Arabidopsis (Arabidopsis thaliana) inflorescence stems. We examined the molecular mechanisms underlying this response and our findings support the Blaauw theory (Blaauw, 1919), suggesting rapid differential growth through unilateral photomorphogenic growth inhibition. UVR8-dependent UV-B light perception occurs mainly in the epidermis and cortex, but deeper tissues such as endodermis can also contribute. Within stems, a spatial difference of UVR8 signal causes a transcript and protein increase of transcription factors ELONGATED HYPOCOTYL5 (HY5) and its homolog HY5 HOMOLOG at the UV-B–exposed side. The irradiated side shows (1) strong activation of flavonoid synthesis genes and flavonoid accumulation; (2) increased gibberellin (GA)2-oxidase expression, diminished GA1 levels, and accumulation of the DELLA protein REPRESSOR OF GA1; and (3) increased expression of the auxin transport regulator PINOID, contributing to diminished auxin signaling. Together, the data suggest a mechanism of phototropin-independent inflorescence phototropism through multiple, locally UVR8-regulated hormone pathways.
Fil: Vanhaelewyn, Lucas. University of Ghent; Bélgica
Fil: Viczián, András. Institute of Plant Biology, Biological Research Centre; Hungría
Fil: Prinsen, Els. Universiteit Antwerp; Bélgica
Fil: Bernula, Péter. Institute of Plant Biology, Biological Research Centre; Hungría. University of Szeged; Hungría
Fil: Serrano, Alejandro Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina
Fil: Arana, Maria Veronica. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Ballare, Carlos Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Nagy, Ferenc. Institute of Plant Biology, Biological Research Centre; Hungría
Fil: Van Der Straeten, Dominique. University of Ghent; Bélgica
Fil: Vandenbussche, Filip. University of Ghent; Bélgica
Materia
UV
UV-B–Induced
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/151150
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Differential UVR8 Signal across the Stem Controls UV-B-Induced Inflorescence PhototropismVanhaelewyn, LucasViczián, AndrásPrinsen, ElsBernula, PéterSerrano, Alejandro MiguelArana, Maria VeronicaBallare, Carlos LuisNagy, FerencVan Der Straeten, DominiqueVandenbussche, FilipUVUV-B–Inducedhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In the course of evolution, plants have developed mechanisms that orient their organs toward the incoming light. At the seedling stage, positive phototropism is mainly regulated by phototropin photoreceptors in blue and UV wavelengths. Contrasting with this, we report that UV RESISTANCE LOCUS8 (UVR8) serves as the predominant photoreceptor of UVB–induced phototropic responses in Arabidopsis (Arabidopsis thaliana) inflorescence stems. We examined the molecular mechanisms underlying this response and our findings support the Blaauw theory (Blaauw, 1919), suggesting rapid differential growth through unilateral photomorphogenic growth inhibition. UVR8-dependent UV-B light perception occurs mainly in the epidermis and cortex, but deeper tissues such as endodermis can also contribute. Within stems, a spatial difference of UVR8 signal causes a transcript and protein increase of transcription factors ELONGATED HYPOCOTYL5 (HY5) and its homolog HY5 HOMOLOG at the UV-B–exposed side. The irradiated side shows (1) strong activation of flavonoid synthesis genes and flavonoid accumulation; (2) increased gibberellin (GA)2-oxidase expression, diminished GA1 levels, and accumulation of the DELLA protein REPRESSOR OF GA1; and (3) increased expression of the auxin transport regulator PINOID, contributing to diminished auxin signaling. Together, the data suggest a mechanism of phototropin-independent inflorescence phototropism through multiple, locally UVR8-regulated hormone pathways.Fil: Vanhaelewyn, Lucas. University of Ghent; BélgicaFil: Viczián, András. Institute of Plant Biology, Biological Research Centre; HungríaFil: Prinsen, Els. Universiteit Antwerp; BélgicaFil: Bernula, Péter. Institute of Plant Biology, Biological Research Centre; Hungría. University of Szeged; HungríaFil: Serrano, Alejandro Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; ArgentinaFil: Arana, Maria Veronica. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: Ballare, Carlos Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Nagy, Ferenc. Institute of Plant Biology, Biological Research Centre; HungríaFil: Van Der Straeten, Dominique. University of Ghent; BélgicaFil: Vandenbussche, Filip. University of Ghent; BélgicaAmerican Society of Plant Biologist2019-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/151150Vanhaelewyn, Lucas; Viczián, András; Prinsen, Els; Bernula, Péter; Serrano, Alejandro Miguel; et al.; Differential UVR8 Signal across the Stem Controls UV-B-Induced Inflorescence Phototropism; American Society of Plant Biologist; Plant Cell; 31; 9; 9-2019; 2070-20881532-298XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1105/tpc.18.00929info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/plcell/article/31/9/2070/5985742info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:02:24Zoai:ri.conicet.gov.ar:11336/151150instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:02:24.664CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Differential UVR8 Signal across the Stem Controls UV-B-Induced Inflorescence Phototropism
title Differential UVR8 Signal across the Stem Controls UV-B-Induced Inflorescence Phototropism
spellingShingle Differential UVR8 Signal across the Stem Controls UV-B-Induced Inflorescence Phototropism
Vanhaelewyn, Lucas
UV
UV-B–Induced
title_short Differential UVR8 Signal across the Stem Controls UV-B-Induced Inflorescence Phototropism
title_full Differential UVR8 Signal across the Stem Controls UV-B-Induced Inflorescence Phototropism
title_fullStr Differential UVR8 Signal across the Stem Controls UV-B-Induced Inflorescence Phototropism
title_full_unstemmed Differential UVR8 Signal across the Stem Controls UV-B-Induced Inflorescence Phototropism
title_sort Differential UVR8 Signal across the Stem Controls UV-B-Induced Inflorescence Phototropism
dc.creator.none.fl_str_mv Vanhaelewyn, Lucas
Viczián, András
Prinsen, Els
Bernula, Péter
Serrano, Alejandro Miguel
Arana, Maria Veronica
Ballare, Carlos Luis
Nagy, Ferenc
Van Der Straeten, Dominique
Vandenbussche, Filip
author Vanhaelewyn, Lucas
author_facet Vanhaelewyn, Lucas
Viczián, András
Prinsen, Els
Bernula, Péter
Serrano, Alejandro Miguel
Arana, Maria Veronica
Ballare, Carlos Luis
Nagy, Ferenc
Van Der Straeten, Dominique
Vandenbussche, Filip
author_role author
author2 Viczián, András
Prinsen, Els
Bernula, Péter
Serrano, Alejandro Miguel
Arana, Maria Veronica
Ballare, Carlos Luis
Nagy, Ferenc
Van Der Straeten, Dominique
Vandenbussche, Filip
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv UV
UV-B–Induced
topic UV
UV-B–Induced
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In the course of evolution, plants have developed mechanisms that orient their organs toward the incoming light. At the seedling stage, positive phototropism is mainly regulated by phototropin photoreceptors in blue and UV wavelengths. Contrasting with this, we report that UV RESISTANCE LOCUS8 (UVR8) serves as the predominant photoreceptor of UVB–induced phototropic responses in Arabidopsis (Arabidopsis thaliana) inflorescence stems. We examined the molecular mechanisms underlying this response and our findings support the Blaauw theory (Blaauw, 1919), suggesting rapid differential growth through unilateral photomorphogenic growth inhibition. UVR8-dependent UV-B light perception occurs mainly in the epidermis and cortex, but deeper tissues such as endodermis can also contribute. Within stems, a spatial difference of UVR8 signal causes a transcript and protein increase of transcription factors ELONGATED HYPOCOTYL5 (HY5) and its homolog HY5 HOMOLOG at the UV-B–exposed side. The irradiated side shows (1) strong activation of flavonoid synthesis genes and flavonoid accumulation; (2) increased gibberellin (GA)2-oxidase expression, diminished GA1 levels, and accumulation of the DELLA protein REPRESSOR OF GA1; and (3) increased expression of the auxin transport regulator PINOID, contributing to diminished auxin signaling. Together, the data suggest a mechanism of phototropin-independent inflorescence phototropism through multiple, locally UVR8-regulated hormone pathways.
Fil: Vanhaelewyn, Lucas. University of Ghent; Bélgica
Fil: Viczián, András. Institute of Plant Biology, Biological Research Centre; Hungría
Fil: Prinsen, Els. Universiteit Antwerp; Bélgica
Fil: Bernula, Péter. Institute of Plant Biology, Biological Research Centre; Hungría. University of Szeged; Hungría
Fil: Serrano, Alejandro Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina
Fil: Arana, Maria Veronica. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Ballare, Carlos Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Nagy, Ferenc. Institute of Plant Biology, Biological Research Centre; Hungría
Fil: Van Der Straeten, Dominique. University of Ghent; Bélgica
Fil: Vandenbussche, Filip. University of Ghent; Bélgica
description In the course of evolution, plants have developed mechanisms that orient their organs toward the incoming light. At the seedling stage, positive phototropism is mainly regulated by phototropin photoreceptors in blue and UV wavelengths. Contrasting with this, we report that UV RESISTANCE LOCUS8 (UVR8) serves as the predominant photoreceptor of UVB–induced phototropic responses in Arabidopsis (Arabidopsis thaliana) inflorescence stems. We examined the molecular mechanisms underlying this response and our findings support the Blaauw theory (Blaauw, 1919), suggesting rapid differential growth through unilateral photomorphogenic growth inhibition. UVR8-dependent UV-B light perception occurs mainly in the epidermis and cortex, but deeper tissues such as endodermis can also contribute. Within stems, a spatial difference of UVR8 signal causes a transcript and protein increase of transcription factors ELONGATED HYPOCOTYL5 (HY5) and its homolog HY5 HOMOLOG at the UV-B–exposed side. The irradiated side shows (1) strong activation of flavonoid synthesis genes and flavonoid accumulation; (2) increased gibberellin (GA)2-oxidase expression, diminished GA1 levels, and accumulation of the DELLA protein REPRESSOR OF GA1; and (3) increased expression of the auxin transport regulator PINOID, contributing to diminished auxin signaling. Together, the data suggest a mechanism of phototropin-independent inflorescence phototropism through multiple, locally UVR8-regulated hormone pathways.
publishDate 2019
dc.date.none.fl_str_mv 2019-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/151150
Vanhaelewyn, Lucas; Viczián, András; Prinsen, Els; Bernula, Péter; Serrano, Alejandro Miguel; et al.; Differential UVR8 Signal across the Stem Controls UV-B-Induced Inflorescence Phototropism; American Society of Plant Biologist; Plant Cell; 31; 9; 9-2019; 2070-2088
1532-298X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/151150
identifier_str_mv Vanhaelewyn, Lucas; Viczián, András; Prinsen, Els; Bernula, Péter; Serrano, Alejandro Miguel; et al.; Differential UVR8 Signal across the Stem Controls UV-B-Induced Inflorescence Phototropism; American Society of Plant Biologist; Plant Cell; 31; 9; 9-2019; 2070-2088
1532-298X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1105/tpc.18.00929
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/plcell/article/31/9/2070/5985742
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Plant Biologist
publisher.none.fl_str_mv American Society of Plant Biologist
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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