Differential UVR8 Signal across the Stem Controls UV-B–Induced Inflorescence Phototropism

Autores
Vanhaelewyn, Lucas; Viczián, András; Prinsen, Els; Bernula, Péter; Serrano, Alejandro Miguel; Arana, María Veronica; Ballaré, Carlos Luis; Nagy, Ferenc; Van Der Straeten, Dominique; Vandenbussche, Filip
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the course of evolution, plants have developed mechanisms that orient their organs toward the incoming light. At the seedling stage, positive phototropism is mainly regulated by phototropin photoreceptors in blue and UV wavelengths. Contrasting with this, we report that UV RESISTANCE LOCUS8 (UVR8) serves as the predominant photoreceptor of UVB– induced phototropic responses in Arabidopsis (Arabidopsis thaliana) inflorescence stems. We examined the molecular mechanisms underlying this response and our findings support the Blaauw theory (Blaauw, 1919), suggesting rapid differential growth through unilateral photomorphogenic growth inhibition. UVR8-dependent UV-B light perception occurs mainly in the epidermis and cortex, but deeper tissues such as endodermis can also contribute. Within stems, a spatial difference of UVR8 signal causes a transcript and protein increase of transcription factors ELONGATED HYPOCOTYL5 (HY5) and its homolog HY5 HOMOLOG at the UV-B–exposed side. The irradiated side shows (1) strong activation of flavonoid synthesis genes and flavonoid accumulation; (2) increased gibberellin (GA)2-oxidase expression, diminished GA1 levels, and accumulation of the DELLA protein REPRESSOR OF GA1; and (3) increased expression of the auxin transport regulator PINOID, contributing to diminished auxin signaling. Together, the data suggest a mechanism of phototropin-independent inflorescence phototropism through multiple, locally UVR8-regulated hormone pathways .
Estación Experimental Agropecuaria Bariloche
Fil: Vanhaelewyn, Lucas. Ghent University. Faculty of Sciences. Department of Biology. Laboratory of Functional Plant Biology; Bélgica
Fil: Viczián, András. Institute of Plant Biology. Biological Research Centre; Hungría
Fil: Prinsen, Els. University of Antwerp. Department of Biology; Bélgica
Fil: Bernula, Péter. Institute of Plant Biology. Biological Research Centre. University of Szeged. Faculty of Science and Informatics; Hungría
Fil: Serrano, Alejandro Miguel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto Argentino de Investigaciones de Zonas Aridas (IADIZA); Argentina
Fil: Arana, María Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Ballaré, Carlos Luis. Universidad Nacional de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiologicas y Ecologicas Vinculadas a la Agricultura (IFEVA); Argentina
Fil: Nagy, Ferenc. Institute of Plant Biology. Biological Research Centre; Hungría
Fil: Van Der Straeten, Dominique. Ghent University. Faculty of Sciences. Department of Biology. Laboratory of Functional Plant Biology; Bélgica
Fil: Vandenbussche, Filip. Ghent University. Faculty of Sciences. Department of Biology. Laboratory of Functional Plant Biology; Bélgica
Fuente
The Plant Cell 31: 2070–2088 (Septiembre 2019)
Materia
Fisiología Vegetal
Fototropismo
Arabidopsis Thaliana
Fotorreceptores
Plant Physiology
Phototropism
Photoreceptors
UVR8
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/7126
Acceder
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oai_identifier_str oai:localhost:20.500.12123/7126
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Differential UVR8 Signal across the Stem Controls UV-B–Induced Inflorescence PhototropismVanhaelewyn, LucasViczián, AndrásPrinsen, ElsBernula, PéterSerrano, Alejandro MiguelArana, María VeronicaBallaré, Carlos LuisNagy, FerencVan Der Straeten, DominiqueVandenbussche, FilipFisiología VegetalFototropismoArabidopsis ThalianaFotorreceptoresPlant PhysiologyPhototropismPhotoreceptorsUVR8In the course of evolution, plants have developed mechanisms that orient their organs toward the incoming light. At the seedling stage, positive phototropism is mainly regulated by phototropin photoreceptors in blue and UV wavelengths. Contrasting with this, we report that UV RESISTANCE LOCUS8 (UVR8) serves as the predominant photoreceptor of UVB– induced phototropic responses in Arabidopsis (Arabidopsis thaliana) inflorescence stems. We examined the molecular mechanisms underlying this response and our findings support the Blaauw theory (Blaauw, 1919), suggesting rapid differential growth through unilateral photomorphogenic growth inhibition. UVR8-dependent UV-B light perception occurs mainly in the epidermis and cortex, but deeper tissues such as endodermis can also contribute. Within stems, a spatial difference of UVR8 signal causes a transcript and protein increase of transcription factors ELONGATED HYPOCOTYL5 (HY5) and its homolog HY5 HOMOLOG at the UV-B–exposed side. The irradiated side shows (1) strong activation of flavonoid synthesis genes and flavonoid accumulation; (2) increased gibberellin (GA)2-oxidase expression, diminished GA1 levels, and accumulation of the DELLA protein REPRESSOR OF GA1; and (3) increased expression of the auxin transport regulator PINOID, contributing to diminished auxin signaling. Together, the data suggest a mechanism of phototropin-independent inflorescence phototropism through multiple, locally UVR8-regulated hormone pathways .Estación Experimental Agropecuaria BarilocheFil: Vanhaelewyn, Lucas. Ghent University. Faculty of Sciences. Department of Biology. Laboratory of Functional Plant Biology; BélgicaFil: Viczián, András. Institute of Plant Biology. Biological Research Centre; HungríaFil: Prinsen, Els. University of Antwerp. Department of Biology; BélgicaFil: Bernula, Péter. Institute of Plant Biology. Biological Research Centre. University of Szeged. Faculty of Science and Informatics; HungríaFil: Serrano, Alejandro Miguel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto Argentino de Investigaciones de Zonas Aridas (IADIZA); ArgentinaFil: Arana, María Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: Ballaré, Carlos Luis. Universidad Nacional de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiologicas y Ecologicas Vinculadas a la Agricultura (IFEVA); ArgentinaFil: Nagy, Ferenc. Institute of Plant Biology. Biological Research Centre; HungríaFil: Van Der Straeten, Dominique. Ghent University. Faculty of Sciences. Department of Biology. Laboratory of Functional Plant Biology; BélgicaFil: Vandenbussche, Filip. Ghent University. Faculty of Sciences. Department of Biology. Laboratory of Functional Plant Biology; BélgicaThe American Society of Plant Biologists2020-04-22T18:37:31Z2020-04-22T18:37:31Z2019-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/7126http://www.plantcell.org/content/31/9/20701040-46511532-298Xhttps://doi.org/10.1105/tpc.18.00929The Plant Cell 31: 2070–2088 (Septiembre 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:48:12Zoai:localhost:20.500.12123/7126instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:48:12.534INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Differential UVR8 Signal across the Stem Controls UV-B–Induced Inflorescence Phototropism
title Differential UVR8 Signal across the Stem Controls UV-B–Induced Inflorescence Phototropism
spellingShingle Differential UVR8 Signal across the Stem Controls UV-B–Induced Inflorescence Phototropism
Vanhaelewyn, Lucas
Fisiología Vegetal
Fototropismo
Arabidopsis Thaliana
Fotorreceptores
Plant Physiology
Phototropism
Photoreceptors
UVR8
title_short Differential UVR8 Signal across the Stem Controls UV-B–Induced Inflorescence Phototropism
title_full Differential UVR8 Signal across the Stem Controls UV-B–Induced Inflorescence Phototropism
title_fullStr Differential UVR8 Signal across the Stem Controls UV-B–Induced Inflorescence Phototropism
title_full_unstemmed Differential UVR8 Signal across the Stem Controls UV-B–Induced Inflorescence Phototropism
title_sort Differential UVR8 Signal across the Stem Controls UV-B–Induced Inflorescence Phototropism
dc.creator.none.fl_str_mv Vanhaelewyn, Lucas
Viczián, András
Prinsen, Els
Bernula, Péter
Serrano, Alejandro Miguel
Arana, María Veronica
Ballaré, Carlos Luis
Nagy, Ferenc
Van Der Straeten, Dominique
Vandenbussche, Filip
author Vanhaelewyn, Lucas
author_facet Vanhaelewyn, Lucas
Viczián, András
Prinsen, Els
Bernula, Péter
Serrano, Alejandro Miguel
Arana, María Veronica
Ballaré, Carlos Luis
Nagy, Ferenc
Van Der Straeten, Dominique
Vandenbussche, Filip
author_role author
author2 Viczián, András
Prinsen, Els
Bernula, Péter
Serrano, Alejandro Miguel
Arana, María Veronica
Ballaré, Carlos Luis
Nagy, Ferenc
Van Der Straeten, Dominique
Vandenbussche, Filip
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Fisiología Vegetal
Fototropismo
Arabidopsis Thaliana
Fotorreceptores
Plant Physiology
Phototropism
Photoreceptors
UVR8
topic Fisiología Vegetal
Fototropismo
Arabidopsis Thaliana
Fotorreceptores
Plant Physiology
Phototropism
Photoreceptors
UVR8
dc.description.none.fl_txt_mv In the course of evolution, plants have developed mechanisms that orient their organs toward the incoming light. At the seedling stage, positive phototropism is mainly regulated by phototropin photoreceptors in blue and UV wavelengths. Contrasting with this, we report that UV RESISTANCE LOCUS8 (UVR8) serves as the predominant photoreceptor of UVB– induced phototropic responses in Arabidopsis (Arabidopsis thaliana) inflorescence stems. We examined the molecular mechanisms underlying this response and our findings support the Blaauw theory (Blaauw, 1919), suggesting rapid differential growth through unilateral photomorphogenic growth inhibition. UVR8-dependent UV-B light perception occurs mainly in the epidermis and cortex, but deeper tissues such as endodermis can also contribute. Within stems, a spatial difference of UVR8 signal causes a transcript and protein increase of transcription factors ELONGATED HYPOCOTYL5 (HY5) and its homolog HY5 HOMOLOG at the UV-B–exposed side. The irradiated side shows (1) strong activation of flavonoid synthesis genes and flavonoid accumulation; (2) increased gibberellin (GA)2-oxidase expression, diminished GA1 levels, and accumulation of the DELLA protein REPRESSOR OF GA1; and (3) increased expression of the auxin transport regulator PINOID, contributing to diminished auxin signaling. Together, the data suggest a mechanism of phototropin-independent inflorescence phototropism through multiple, locally UVR8-regulated hormone pathways .
Estación Experimental Agropecuaria Bariloche
Fil: Vanhaelewyn, Lucas. Ghent University. Faculty of Sciences. Department of Biology. Laboratory of Functional Plant Biology; Bélgica
Fil: Viczián, András. Institute of Plant Biology. Biological Research Centre; Hungría
Fil: Prinsen, Els. University of Antwerp. Department of Biology; Bélgica
Fil: Bernula, Péter. Institute of Plant Biology. Biological Research Centre. University of Szeged. Faculty of Science and Informatics; Hungría
Fil: Serrano, Alejandro Miguel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto Argentino de Investigaciones de Zonas Aridas (IADIZA); Argentina
Fil: Arana, María Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Ballaré, Carlos Luis. Universidad Nacional de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiologicas y Ecologicas Vinculadas a la Agricultura (IFEVA); Argentina
Fil: Nagy, Ferenc. Institute of Plant Biology. Biological Research Centre; Hungría
Fil: Van Der Straeten, Dominique. Ghent University. Faculty of Sciences. Department of Biology. Laboratory of Functional Plant Biology; Bélgica
Fil: Vandenbussche, Filip. Ghent University. Faculty of Sciences. Department of Biology. Laboratory of Functional Plant Biology; Bélgica
description In the course of evolution, plants have developed mechanisms that orient their organs toward the incoming light. At the seedling stage, positive phototropism is mainly regulated by phototropin photoreceptors in blue and UV wavelengths. Contrasting with this, we report that UV RESISTANCE LOCUS8 (UVR8) serves as the predominant photoreceptor of UVB– induced phototropic responses in Arabidopsis (Arabidopsis thaliana) inflorescence stems. We examined the molecular mechanisms underlying this response and our findings support the Blaauw theory (Blaauw, 1919), suggesting rapid differential growth through unilateral photomorphogenic growth inhibition. UVR8-dependent UV-B light perception occurs mainly in the epidermis and cortex, but deeper tissues such as endodermis can also contribute. Within stems, a spatial difference of UVR8 signal causes a transcript and protein increase of transcription factors ELONGATED HYPOCOTYL5 (HY5) and its homolog HY5 HOMOLOG at the UV-B–exposed side. The irradiated side shows (1) strong activation of flavonoid synthesis genes and flavonoid accumulation; (2) increased gibberellin (GA)2-oxidase expression, diminished GA1 levels, and accumulation of the DELLA protein REPRESSOR OF GA1; and (3) increased expression of the auxin transport regulator PINOID, contributing to diminished auxin signaling. Together, the data suggest a mechanism of phototropin-independent inflorescence phototropism through multiple, locally UVR8-regulated hormone pathways .
publishDate 2019
dc.date.none.fl_str_mv 2019-09
2020-04-22T18:37:31Z
2020-04-22T18:37:31Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/7126
http://www.plantcell.org/content/31/9/2070
1040-4651
1532-298X
https://doi.org/10.1105/tpc.18.00929
url http://hdl.handle.net/20.500.12123/7126
http://www.plantcell.org/content/31/9/2070
https://doi.org/10.1105/tpc.18.00929
identifier_str_mv 1040-4651
1532-298X
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv The American Society of Plant Biologists
publisher.none.fl_str_mv The American Society of Plant Biologists
dc.source.none.fl_str_mv The Plant Cell 31: 2070–2088 (Septiembre 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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