Phospholipid remodeling in dopaminergic neurons: role of α-synuclein variants and iron overload

Autores
Sánchez Campos, Sofía; Salvador, Gabriela Alejandra
Año de publicación
2013
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Increased levels of α-synuclein (α-syn) and iron-overload are pathognomonic signs of dopaminergic neurons in Parkinson’s disease (PD) patients. Moreover, iron and fatty acid (FA) availability are predisposing factors for pathological α-syn aggregation. In this work, we characterized the phospholipid remodeling pathways that regulate FA availability in dopaminergic neurons overexpressing α-syn variants (WT and A53T) and exposed to iron-overload. Increased cellular oxidant and lipid peroxidation levels were observed in dopaminergic neurons exposed to iron-overload. The inhibition of calcium-independent phospholipase A2 (deacylation pathway) provoked an increase in the extent of cellular damage induced by iron-overload. In this connection, phospholipid acylation was differentially affected by iron overload and the presence of α- syn variants. FA incorporation into phosphatidylcholine (PC) and phosphatidylethanolamine (PE) was increased in dopaminergic neurons harboring WT α-syn. This acylation profile was not altered by iron-overload. Neurons expressing A53T α-syn (a variant present in autosomic dominant PD and with high iron affinity) showed a diminished FA esterification in PC and PE. This effect was enhanced in iron overloaded neurons. Our results show that FA availability is differentially regulated by α-syn variants and iron overload in this in vitro model of PD
Fil: Sánchez Campos, Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular. Molecular mechanisms in cell signaling and gene expression
Buenos Aires
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Materia
NEURODEGENERATION
OXIDATIVE STRESS
IRON
ACYLTRANSFERASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/246944
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Phospholipid remodeling in dopaminergic neurons: role of α-synuclein variants and iron overloadSánchez Campos, SofíaSalvador, Gabriela AlejandraNEURODEGENERATIONOXIDATIVE STRESSIRONACYLTRANSFERASEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Increased levels of α-synuclein (α-syn) and iron-overload are pathognomonic signs of dopaminergic neurons in Parkinson’s disease (PD) patients. Moreover, iron and fatty acid (FA) availability are predisposing factors for pathological α-syn aggregation. In this work, we characterized the phospholipid remodeling pathways that regulate FA availability in dopaminergic neurons overexpressing α-syn variants (WT and A53T) and exposed to iron-overload. Increased cellular oxidant and lipid peroxidation levels were observed in dopaminergic neurons exposed to iron-overload. The inhibition of calcium-independent phospholipase A2 (deacylation pathway) provoked an increase in the extent of cellular damage induced by iron-overload. In this connection, phospholipid acylation was differentially affected by iron overload and the presence of α- syn variants. FA incorporation into phosphatidylcholine (PC) and phosphatidylethanolamine (PE) was increased in dopaminergic neurons harboring WT α-syn. This acylation profile was not altered by iron-overload. Neurons expressing A53T α-syn (a variant present in autosomic dominant PD and with high iron affinity) showed a diminished FA esterification in PC and PE. This effect was enhanced in iron overloaded neurons. Our results show that FA availability is differentially regulated by α-syn variants and iron overload in this in vitro model of PDFil: Sánchez Campos, Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaSociedad Argentina de Investigación en Bioquímica y Biología Molecular. Molecular mechanisms in cell signaling and gene expressionBuenos AiresArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularInstituto de Histología y Embriología “Dr. Mario H. Burgos”2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/246944Phospholipid remodeling in dopaminergic neurons: role of α-synuclein variants and iron overload; Sociedad Argentina de Investigación en Bioquímica y Biología Molecular. Molecular mechanisms in cell signaling and gene expression; Buenos Aires; Argentina; 2013; 40-400327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.saib.org.ar/sites/default/files/BIOCELL-SAIB-2013.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:32:33Zoai:ri.conicet.gov.ar:11336/246944instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:32:33.979CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Phospholipid remodeling in dopaminergic neurons: role of α-synuclein variants and iron overload
title Phospholipid remodeling in dopaminergic neurons: role of α-synuclein variants and iron overload
spellingShingle Phospholipid remodeling in dopaminergic neurons: role of α-synuclein variants and iron overload
Sánchez Campos, Sofía
NEURODEGENERATION
OXIDATIVE STRESS
IRON
ACYLTRANSFERASE
title_short Phospholipid remodeling in dopaminergic neurons: role of α-synuclein variants and iron overload
title_full Phospholipid remodeling in dopaminergic neurons: role of α-synuclein variants and iron overload
title_fullStr Phospholipid remodeling in dopaminergic neurons: role of α-synuclein variants and iron overload
title_full_unstemmed Phospholipid remodeling in dopaminergic neurons: role of α-synuclein variants and iron overload
title_sort Phospholipid remodeling in dopaminergic neurons: role of α-synuclein variants and iron overload
dc.creator.none.fl_str_mv Sánchez Campos, Sofía
Salvador, Gabriela Alejandra
author Sánchez Campos, Sofía
author_facet Sánchez Campos, Sofía
Salvador, Gabriela Alejandra
author_role author
author2 Salvador, Gabriela Alejandra
author2_role author
dc.subject.none.fl_str_mv NEURODEGENERATION
OXIDATIVE STRESS
IRON
ACYLTRANSFERASE
topic NEURODEGENERATION
OXIDATIVE STRESS
IRON
ACYLTRANSFERASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Increased levels of α-synuclein (α-syn) and iron-overload are pathognomonic signs of dopaminergic neurons in Parkinson’s disease (PD) patients. Moreover, iron and fatty acid (FA) availability are predisposing factors for pathological α-syn aggregation. In this work, we characterized the phospholipid remodeling pathways that regulate FA availability in dopaminergic neurons overexpressing α-syn variants (WT and A53T) and exposed to iron-overload. Increased cellular oxidant and lipid peroxidation levels were observed in dopaminergic neurons exposed to iron-overload. The inhibition of calcium-independent phospholipase A2 (deacylation pathway) provoked an increase in the extent of cellular damage induced by iron-overload. In this connection, phospholipid acylation was differentially affected by iron overload and the presence of α- syn variants. FA incorporation into phosphatidylcholine (PC) and phosphatidylethanolamine (PE) was increased in dopaminergic neurons harboring WT α-syn. This acylation profile was not altered by iron-overload. Neurons expressing A53T α-syn (a variant present in autosomic dominant PD and with high iron affinity) showed a diminished FA esterification in PC and PE. This effect was enhanced in iron overloaded neurons. Our results show that FA availability is differentially regulated by α-syn variants and iron overload in this in vitro model of PD
Fil: Sánchez Campos, Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular. Molecular mechanisms in cell signaling and gene expression
Buenos Aires
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
description Increased levels of α-synuclein (α-syn) and iron-overload are pathognomonic signs of dopaminergic neurons in Parkinson’s disease (PD) patients. Moreover, iron and fatty acid (FA) availability are predisposing factors for pathological α-syn aggregation. In this work, we characterized the phospholipid remodeling pathways that regulate FA availability in dopaminergic neurons overexpressing α-syn variants (WT and A53T) and exposed to iron-overload. Increased cellular oxidant and lipid peroxidation levels were observed in dopaminergic neurons exposed to iron-overload. The inhibition of calcium-independent phospholipase A2 (deacylation pathway) provoked an increase in the extent of cellular damage induced by iron-overload. In this connection, phospholipid acylation was differentially affected by iron overload and the presence of α- syn variants. FA incorporation into phosphatidylcholine (PC) and phosphatidylethanolamine (PE) was increased in dopaminergic neurons harboring WT α-syn. This acylation profile was not altered by iron-overload. Neurons expressing A53T α-syn (a variant present in autosomic dominant PD and with high iron affinity) showed a diminished FA esterification in PC and PE. This effect was enhanced in iron overloaded neurons. Our results show that FA availability is differentially regulated by α-syn variants and iron overload in this in vitro model of PD
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/246944
Phospholipid remodeling in dopaminergic neurons: role of α-synuclein variants and iron overload; Sociedad Argentina de Investigación en Bioquímica y Biología Molecular. Molecular mechanisms in cell signaling and gene expression; Buenos Aires; Argentina; 2013; 40-40
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/246944
identifier_str_mv Phospholipid remodeling in dopaminergic neurons: role of α-synuclein variants and iron overload; Sociedad Argentina de Investigación en Bioquímica y Biología Molecular. Molecular mechanisms in cell signaling and gene expression; Buenos Aires; Argentina; 2013; 40-40
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.saib.org.ar/sites/default/files/BIOCELL-SAIB-2013.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Instituto de Histología y Embriología “Dr. Mario H. Burgos”
publisher.none.fl_str_mv Instituto de Histología y Embriología “Dr. Mario H. Burgos”
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.976206

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