A53T alpha-syn regulates triacylglycerol content in dopaminergic neurons exposed to oxidative stress

Autores
Sánchez Campos, Sofía; Salvador, Gabriela Alejandra
Año de publicación
2014
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Iron-induced oxidative stress and pathological α-synuclein (α-syn) aggregation contribute to the loss of dopaminergic neurons in Parkinson Disease (PD). In this work, we characterized the status of lipid metabolism in N27 dopaminergic neurons and in neurons stably expressing A53T α-syn (a dominant mutation found in familial early onset PD) exposed to iron-induced injury. N27 dopaminergic neurons incubated with iron for 24 hs (Fe, 1mM) displayed increased levels of reactive oxygen species (ROS), lipid peroxidation and elevated plasma membrane permeability. A different lipid acylation profile was observed in fatty acid prelabeled N27 neurons exposed to Fe. [3H] arachidonic acid (AA) uptake was increased in triacylglycerols (TAG) whereas the incorporation of [3H] oleic acid into TAG showed no changes. AA incorporation increased in phosphatidylcholine and diminished in phosphatidylserine and phosphatidylinositol. Coincidently, TAG content was 40 % higher in Fe-exposed neurons than in controls. The accumulation of TAG was also observed by the appearance of Nile red positive lipid bodies. On the contrary, A53T α-syn neurons exposed to iron-injury showed no increase in TAG levels and diminished ROS content. Our results suggest that TAG accumulation could be a mechanism for AA storage and that α-syn could act as an iron scavenger during oxidative stress in dopaminergic neurons.
Fil: Sánchez Campos, Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Salvador, Gabriela Alejandra. Universidad Nacional del Sur; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
50th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; L Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Rosario
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Materia
OXIDATIVE STRESS
IRON
ALPHA-SYNUCLEIN
TRIACYLGLYCEROL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/222188
Acceder
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network_name_str CONICET Digital (CONICET)
spelling A53T alpha-syn regulates triacylglycerol content in dopaminergic neurons exposed to oxidative stressSánchez Campos, SofíaSalvador, Gabriela AlejandraOXIDATIVE STRESSIRONALPHA-SYNUCLEINTRIACYLGLYCEROLhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Iron-induced oxidative stress and pathological α-synuclein (α-syn) aggregation contribute to the loss of dopaminergic neurons in Parkinson Disease (PD). In this work, we characterized the status of lipid metabolism in N27 dopaminergic neurons and in neurons stably expressing A53T α-syn (a dominant mutation found in familial early onset PD) exposed to iron-induced injury. N27 dopaminergic neurons incubated with iron for 24 hs (Fe, 1mM) displayed increased levels of reactive oxygen species (ROS), lipid peroxidation and elevated plasma membrane permeability. A different lipid acylation profile was observed in fatty acid prelabeled N27 neurons exposed to Fe. [3H] arachidonic acid (AA) uptake was increased in triacylglycerols (TAG) whereas the incorporation of [3H] oleic acid into TAG showed no changes. AA incorporation increased in phosphatidylcholine and diminished in phosphatidylserine and phosphatidylinositol. Coincidently, TAG content was 40 % higher in Fe-exposed neurons than in controls. The accumulation of TAG was also observed by the appearance of Nile red positive lipid bodies. On the contrary, A53T α-syn neurons exposed to iron-injury showed no increase in TAG levels and diminished ROS content. Our results suggest that TAG accumulation could be a mechanism for AA storage and that α-syn could act as an iron scavenger during oxidative stress in dopaminergic neurons.Fil: Sánchez Campos, Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Salvador, Gabriela Alejandra. Universidad Nacional del Sur; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina50th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; L Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología MolecularRosarioArgentinaSociedad Argentina de Investigación Bioquímica y Biología MolecularBiocell2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/222188A53T alpha-syn regulates triacylglycerol content in dopaminergic neurons exposed to oxidative stress; 50th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; L Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Rosario; Argentina; 2014; 124-1250327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v38nSuppl.S/34067/pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:17:41Zoai:ri.conicet.gov.ar:11336/222188instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:17:41.638CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A53T alpha-syn regulates triacylglycerol content in dopaminergic neurons exposed to oxidative stress
title A53T alpha-syn regulates triacylglycerol content in dopaminergic neurons exposed to oxidative stress
spellingShingle A53T alpha-syn regulates triacylglycerol content in dopaminergic neurons exposed to oxidative stress
Sánchez Campos, Sofía
OXIDATIVE STRESS
IRON
ALPHA-SYNUCLEIN
TRIACYLGLYCEROL
title_short A53T alpha-syn regulates triacylglycerol content in dopaminergic neurons exposed to oxidative stress
title_full A53T alpha-syn regulates triacylglycerol content in dopaminergic neurons exposed to oxidative stress
title_fullStr A53T alpha-syn regulates triacylglycerol content in dopaminergic neurons exposed to oxidative stress
title_full_unstemmed A53T alpha-syn regulates triacylglycerol content in dopaminergic neurons exposed to oxidative stress
title_sort A53T alpha-syn regulates triacylglycerol content in dopaminergic neurons exposed to oxidative stress
dc.creator.none.fl_str_mv Sánchez Campos, Sofía
Salvador, Gabriela Alejandra
author Sánchez Campos, Sofía
author_facet Sánchez Campos, Sofía
Salvador, Gabriela Alejandra
author_role author
author2 Salvador, Gabriela Alejandra
author2_role author
dc.subject.none.fl_str_mv OXIDATIVE STRESS
IRON
ALPHA-SYNUCLEIN
TRIACYLGLYCEROL
topic OXIDATIVE STRESS
IRON
ALPHA-SYNUCLEIN
TRIACYLGLYCEROL
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Iron-induced oxidative stress and pathological α-synuclein (α-syn) aggregation contribute to the loss of dopaminergic neurons in Parkinson Disease (PD). In this work, we characterized the status of lipid metabolism in N27 dopaminergic neurons and in neurons stably expressing A53T α-syn (a dominant mutation found in familial early onset PD) exposed to iron-induced injury. N27 dopaminergic neurons incubated with iron for 24 hs (Fe, 1mM) displayed increased levels of reactive oxygen species (ROS), lipid peroxidation and elevated plasma membrane permeability. A different lipid acylation profile was observed in fatty acid prelabeled N27 neurons exposed to Fe. [3H] arachidonic acid (AA) uptake was increased in triacylglycerols (TAG) whereas the incorporation of [3H] oleic acid into TAG showed no changes. AA incorporation increased in phosphatidylcholine and diminished in phosphatidylserine and phosphatidylinositol. Coincidently, TAG content was 40 % higher in Fe-exposed neurons than in controls. The accumulation of TAG was also observed by the appearance of Nile red positive lipid bodies. On the contrary, A53T α-syn neurons exposed to iron-injury showed no increase in TAG levels and diminished ROS content. Our results suggest that TAG accumulation could be a mechanism for AA storage and that α-syn could act as an iron scavenger during oxidative stress in dopaminergic neurons.
Fil: Sánchez Campos, Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Salvador, Gabriela Alejandra. Universidad Nacional del Sur; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
50th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; L Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Rosario
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
description Iron-induced oxidative stress and pathological α-synuclein (α-syn) aggregation contribute to the loss of dopaminergic neurons in Parkinson Disease (PD). In this work, we characterized the status of lipid metabolism in N27 dopaminergic neurons and in neurons stably expressing A53T α-syn (a dominant mutation found in familial early onset PD) exposed to iron-induced injury. N27 dopaminergic neurons incubated with iron for 24 hs (Fe, 1mM) displayed increased levels of reactive oxygen species (ROS), lipid peroxidation and elevated plasma membrane permeability. A different lipid acylation profile was observed in fatty acid prelabeled N27 neurons exposed to Fe. [3H] arachidonic acid (AA) uptake was increased in triacylglycerols (TAG) whereas the incorporation of [3H] oleic acid into TAG showed no changes. AA incorporation increased in phosphatidylcholine and diminished in phosphatidylserine and phosphatidylinositol. Coincidently, TAG content was 40 % higher in Fe-exposed neurons than in controls. The accumulation of TAG was also observed by the appearance of Nile red positive lipid bodies. On the contrary, A53T α-syn neurons exposed to iron-injury showed no increase in TAG levels and diminished ROS content. Our results suggest that TAG accumulation could be a mechanism for AA storage and that α-syn could act as an iron scavenger during oxidative stress in dopaminergic neurons.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/222188
A53T alpha-syn regulates triacylglycerol content in dopaminergic neurons exposed to oxidative stress; 50th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; L Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Rosario; Argentina; 2014; 124-125
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/222188
identifier_str_mv A53T alpha-syn regulates triacylglycerol content in dopaminergic neurons exposed to oxidative stress; 50th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; L Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Rosario; Argentina; 2014; 124-125
0327-9545
1667-5746
CONICET Digital
CONICET
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language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v38nSuppl.S/34067/pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Biocell
publisher.none.fl_str_mv Biocell
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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