Metal-induced oxidative stress activates different lipid signaling pathways in dopaminergic neurons

Autores
Sánchez Campos, Sofía; Rodriguez Diez, Guadalupe; Uranga, Romina Maria; Salvador, Gabriela Alejandra
Año de publicación
2012
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The characterization of the mechanisms mediating the effects of metal-induced oxidative stress on neuronal dysfunction and death is central in the understanding of the pathology of several neurodegenerative disorders such as Parkinson’s disease. In this work, we characterized the cellular responses that operate in dopaminergic neurons (N27 cells) exposed to an overload of transition metals such as iron (Fe, 1 mM), copper (Cu, 10 and 50 µM) or their combination for 24 hs. Under these experimental conditions, reactive oxygen species measured by fluorescence microscopy, and lipid peroxidation levels increased as a function of metal concentration. Maximum levels of lipid peroxides were observed in the presence of Fe + Cu. Cell viability, determined by MTT reduction, strongly decreased in the presence of Cu and with the combination of both metals. Under these experimental conditions, an increase in the levels of Akt phosphorylation in Ser473 was observed. Bcl-2 expression showed the same profile that Akt phosphorylation. In addition, the expression and the activation of the secretory and cytosolic isoforms of phospholipase A2 (PLA2) were differentially affected by metal overload. Our results demonstrate that phospholipid deacylation processes catalyzed by PLA2s and PI3K activation are involved in the response of dopaminergic neurons to metal-induced oxidative stress.
Fil: Sánchez Campos, Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Rodriguez Diez, Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Uranga, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
48th Annual Meeting Argentine Society for Biochemistry and Molecular Biology
Mendoza
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Materia
NEURODEGENERATION
DOPAMINERGIC NEURONS
OXIDATIVE STRESS
PHOSPHOLIPASE A
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/244984
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/244984
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Metal-induced oxidative stress activates different lipid signaling pathways in dopaminergic neuronsSánchez Campos, SofíaRodriguez Diez, GuadalupeUranga, Romina MariaSalvador, Gabriela AlejandraNEURODEGENERATIONDOPAMINERGIC NEURONSOXIDATIVE STRESSPHOSPHOLIPASE Ahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The characterization of the mechanisms mediating the effects of metal-induced oxidative stress on neuronal dysfunction and death is central in the understanding of the pathology of several neurodegenerative disorders such as Parkinson’s disease. In this work, we characterized the cellular responses that operate in dopaminergic neurons (N27 cells) exposed to an overload of transition metals such as iron (Fe, 1 mM), copper (Cu, 10 and 50 µM) or their combination for 24 hs. Under these experimental conditions, reactive oxygen species measured by fluorescence microscopy, and lipid peroxidation levels increased as a function of metal concentration. Maximum levels of lipid peroxides were observed in the presence of Fe + Cu. Cell viability, determined by MTT reduction, strongly decreased in the presence of Cu and with the combination of both metals. Under these experimental conditions, an increase in the levels of Akt phosphorylation in Ser473 was observed. Bcl-2 expression showed the same profile that Akt phosphorylation. In addition, the expression and the activation of the secretory and cytosolic isoforms of phospholipase A2 (PLA2) were differentially affected by metal overload. Our results demonstrate that phospholipid deacylation processes catalyzed by PLA2s and PI3K activation are involved in the response of dopaminergic neurons to metal-induced oxidative stress.Fil: Sánchez Campos, Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Rodriguez Diez, Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Uranga, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina48th Annual Meeting Argentine Society for Biochemistry and Molecular BiologyMendozaArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularInstituto de Histología y Embriología “Dr. Mario H. Burgos”2012info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/244984Metal-induced oxidative stress activates different lipid signaling pathways in dopaminergic neurons; 48th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; Mendoza; Argentina; 2012; 109-1090327-95451667-5746CONICET DigitalCONICETengNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:08:10Zoai:ri.conicet.gov.ar:11336/244984instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:08:10.539CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Metal-induced oxidative stress activates different lipid signaling pathways in dopaminergic neurons
title Metal-induced oxidative stress activates different lipid signaling pathways in dopaminergic neurons
spellingShingle Metal-induced oxidative stress activates different lipid signaling pathways in dopaminergic neurons
Sánchez Campos, Sofía
NEURODEGENERATION
DOPAMINERGIC NEURONS
OXIDATIVE STRESS
PHOSPHOLIPASE A
title_short Metal-induced oxidative stress activates different lipid signaling pathways in dopaminergic neurons
title_full Metal-induced oxidative stress activates different lipid signaling pathways in dopaminergic neurons
title_fullStr Metal-induced oxidative stress activates different lipid signaling pathways in dopaminergic neurons
title_full_unstemmed Metal-induced oxidative stress activates different lipid signaling pathways in dopaminergic neurons
title_sort Metal-induced oxidative stress activates different lipid signaling pathways in dopaminergic neurons
dc.creator.none.fl_str_mv Sánchez Campos, Sofía
Rodriguez Diez, Guadalupe
Uranga, Romina Maria
Salvador, Gabriela Alejandra
author Sánchez Campos, Sofía
author_facet Sánchez Campos, Sofía
Rodriguez Diez, Guadalupe
Uranga, Romina Maria
Salvador, Gabriela Alejandra
author_role author
author2 Rodriguez Diez, Guadalupe
Uranga, Romina Maria
Salvador, Gabriela Alejandra
author2_role author
author
author
dc.subject.none.fl_str_mv NEURODEGENERATION
DOPAMINERGIC NEURONS
OXIDATIVE STRESS
PHOSPHOLIPASE A
topic NEURODEGENERATION
DOPAMINERGIC NEURONS
OXIDATIVE STRESS
PHOSPHOLIPASE A
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The characterization of the mechanisms mediating the effects of metal-induced oxidative stress on neuronal dysfunction and death is central in the understanding of the pathology of several neurodegenerative disorders such as Parkinson’s disease. In this work, we characterized the cellular responses that operate in dopaminergic neurons (N27 cells) exposed to an overload of transition metals such as iron (Fe, 1 mM), copper (Cu, 10 and 50 µM) or their combination for 24 hs. Under these experimental conditions, reactive oxygen species measured by fluorescence microscopy, and lipid peroxidation levels increased as a function of metal concentration. Maximum levels of lipid peroxides were observed in the presence of Fe + Cu. Cell viability, determined by MTT reduction, strongly decreased in the presence of Cu and with the combination of both metals. Under these experimental conditions, an increase in the levels of Akt phosphorylation in Ser473 was observed. Bcl-2 expression showed the same profile that Akt phosphorylation. In addition, the expression and the activation of the secretory and cytosolic isoforms of phospholipase A2 (PLA2) were differentially affected by metal overload. Our results demonstrate that phospholipid deacylation processes catalyzed by PLA2s and PI3K activation are involved in the response of dopaminergic neurons to metal-induced oxidative stress.
Fil: Sánchez Campos, Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Rodriguez Diez, Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Uranga, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
48th Annual Meeting Argentine Society for Biochemistry and Molecular Biology
Mendoza
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
description The characterization of the mechanisms mediating the effects of metal-induced oxidative stress on neuronal dysfunction and death is central in the understanding of the pathology of several neurodegenerative disorders such as Parkinson’s disease. In this work, we characterized the cellular responses that operate in dopaminergic neurons (N27 cells) exposed to an overload of transition metals such as iron (Fe, 1 mM), copper (Cu, 10 and 50 µM) or their combination for 24 hs. Under these experimental conditions, reactive oxygen species measured by fluorescence microscopy, and lipid peroxidation levels increased as a function of metal concentration. Maximum levels of lipid peroxides were observed in the presence of Fe + Cu. Cell viability, determined by MTT reduction, strongly decreased in the presence of Cu and with the combination of both metals. Under these experimental conditions, an increase in the levels of Akt phosphorylation in Ser473 was observed. Bcl-2 expression showed the same profile that Akt phosphorylation. In addition, the expression and the activation of the secretory and cytosolic isoforms of phospholipase A2 (PLA2) were differentially affected by metal overload. Our results demonstrate that phospholipid deacylation processes catalyzed by PLA2s and PI3K activation are involved in the response of dopaminergic neurons to metal-induced oxidative stress.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/244984
Metal-induced oxidative stress activates different lipid signaling pathways in dopaminergic neurons; 48th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; Mendoza; Argentina; 2012; 109-109
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/244984
identifier_str_mv Metal-induced oxidative stress activates different lipid signaling pathways in dopaminergic neurons; 48th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; Mendoza; Argentina; 2012; 109-109
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Instituto de Histología y Embriología “Dr. Mario H. Burgos”
publisher.none.fl_str_mv Instituto de Histología y Embriología “Dr. Mario H. Burgos”
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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