Comparative Proteomic Analyses Between Biofilm-Forming and Non-biofilm-Forming Strains of Corynebacterium pseudotuberculosis Isolated From Goats
- Autores
- Sá, Maria Conceição Aquino de; Marques Da Silva, Wanderson; Rodrigues, Carla Catarine Santos; Rezende, Cristiana Perdigão; Marchioro, Silvana Beutinger; Rocha Filho, José Tadeu Raynal; Sousa, Thiago de Jesus; Oliveira, Helinando Pequeno de; Costa, Mateus Matiuzzi da; Figueiredo, Henrique César Pereira; Portela, Ricardo Dias; Castro, Thiago Luiz de Paula; Azevedo, Vasco; Seyffert, Nubia; Meyer, Roberto
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Caseous lymphadenitis (CLA) is a chronic disease that affects small ruminants and causes economic losses in the associated breeding system. The causative agent of CLA is Corynebacterium pseudotuberculosis, a Gram-positive bacterium that exhibits tropism for external and internal lymph nodes and induces abscess formation in the host. Bacterial communities often produce a biofilm matrix that serves various functions, including protection against hostile environmental conditions, antibiotics, and the host immune response. Although biofilm formation has been reported for C. pseudotuberculosis, not all strains demonstrate this property in culture. In this work, we report the first comparative proteomic analysis of one biofilm-forming (CAPJ4) and one biofilm-non-forming strain (CAP3W) of C. pseudotuberculosis isolated from goats. Bacterial whole cell protein extracts were obtained for mass spectrometry analyses. Using LC-MS/MS, our studies reveal three and four proteins exclusively found in the CAPJ4 and CAP3W proteome, respectively. In addition, label-free quantitative analysis identified 40 proteins showing at-least 2-fold higher values in CAPJ4 compared CAP3W proteome Notably, CAPJ4 differentially synthesized the penicillin-binding protein, which participates in the formation of peptidoglycans. CAPJ4 also exhibited upregulation of N-acetylmuramoyl-L-alanine amidase and galactose-1-phosphate uridylyltransferase, which are involved in biofilm formation and exopolysaccharide biosynthesis. Here, we demonstrate that biofilm formation in C. pseudotuberculosis is likely associated with specific proteins, some of which were previously shown to be associated with virulence and biofilm formation in other organisms. Our findings may drive studies related to the bacterial mechanisms involved in the biofilm formation, in addition to providing targets for the treatment of CLA.
Fil: Sá, Maria Conceição Aquino de. Universidade Federal da Bahia; Brasil
Fil: Marques Da Silva, Wanderson. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Rodrigues, Carla Catarine Santos. Universidade Federal da Bahia; Brasil
Fil: Rezende, Cristiana Perdigão. Universidade Federal de Minas Gerais; Brasil
Fil: Marchioro, Silvana Beutinger. Universidade Federal da Bahia; Brasil
Fil: Rocha Filho, José Tadeu Raynal. Universidade Federal da Bahia; Brasil
Fil: Sousa, Thiago de Jesus. Universidade Federal de Minas Gerais; Brasil
Fil: Oliveira, Helinando Pequeno de. Universidade Federal do Vale do Sao Francisco; Brasil
Fil: Costa, Mateus Matiuzzi da. Universidade Federal do Vale do Sao Francisco; Brasil
Fil: Figueiredo, Henrique César Pereira. Universidade Federal de Minas Gerais; Brasil
Fil: Portela, Ricardo Dias. Universidade Federal da Bahia; Brasil
Fil: Castro, Thiago Luiz de Paula. Universidade Federal da Bahia; Brasil
Fil: Azevedo, Vasco. Universidade Federal de Minas Gerais; Brasil
Fil: Seyffert, Nubia. Universidade Federal da Bahia; Brasil
Fil: Meyer, Roberto. Universidade Federal da Bahia; Brasil - Materia
-
BACTERIAL BIOFILM
CASEOUS LYMPHADENITIS
PROTEOMICS
SMALL RUMINANTS
VIRULENCE FACTORS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/155309
Ver los metadatos del registro completo
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Comparative Proteomic Analyses Between Biofilm-Forming and Non-biofilm-Forming Strains of Corynebacterium pseudotuberculosis Isolated From GoatsSá, Maria Conceição Aquino deMarques Da Silva, WandersonRodrigues, Carla Catarine SantosRezende, Cristiana PerdigãoMarchioro, Silvana BeutingerRocha Filho, José Tadeu RaynalSousa, Thiago de JesusOliveira, Helinando Pequeno deCosta, Mateus Matiuzzi daFigueiredo, Henrique César PereiraPortela, Ricardo DiasCastro, Thiago Luiz de PaulaAzevedo, VascoSeyffert, NubiaMeyer, RobertoBACTERIAL BIOFILMCASEOUS LYMPHADENITISPROTEOMICSSMALL RUMINANTSVIRULENCE FACTORShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Caseous lymphadenitis (CLA) is a chronic disease that affects small ruminants and causes economic losses in the associated breeding system. The causative agent of CLA is Corynebacterium pseudotuberculosis, a Gram-positive bacterium that exhibits tropism for external and internal lymph nodes and induces abscess formation in the host. Bacterial communities often produce a biofilm matrix that serves various functions, including protection against hostile environmental conditions, antibiotics, and the host immune response. Although biofilm formation has been reported for C. pseudotuberculosis, not all strains demonstrate this property in culture. In this work, we report the first comparative proteomic analysis of one biofilm-forming (CAPJ4) and one biofilm-non-forming strain (CAP3W) of C. pseudotuberculosis isolated from goats. Bacterial whole cell protein extracts were obtained for mass spectrometry analyses. Using LC-MS/MS, our studies reveal three and four proteins exclusively found in the CAPJ4 and CAP3W proteome, respectively. In addition, label-free quantitative analysis identified 40 proteins showing at-least 2-fold higher values in CAPJ4 compared CAP3W proteome Notably, CAPJ4 differentially synthesized the penicillin-binding protein, which participates in the formation of peptidoglycans. CAPJ4 also exhibited upregulation of N-acetylmuramoyl-L-alanine amidase and galactose-1-phosphate uridylyltransferase, which are involved in biofilm formation and exopolysaccharide biosynthesis. Here, we demonstrate that biofilm formation in C. pseudotuberculosis is likely associated with specific proteins, some of which were previously shown to be associated with virulence and biofilm formation in other organisms. Our findings may drive studies related to the bacterial mechanisms involved in the biofilm formation, in addition to providing targets for the treatment of CLA.Fil: Sá, Maria Conceição Aquino de. Universidade Federal da Bahia; BrasilFil: Marques Da Silva, Wanderson. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Rodrigues, Carla Catarine Santos. Universidade Federal da Bahia; BrasilFil: Rezende, Cristiana Perdigão. Universidade Federal de Minas Gerais; BrasilFil: Marchioro, Silvana Beutinger. Universidade Federal da Bahia; BrasilFil: Rocha Filho, José Tadeu Raynal. Universidade Federal da Bahia; BrasilFil: Sousa, Thiago de Jesus. Universidade Federal de Minas Gerais; BrasilFil: Oliveira, Helinando Pequeno de. Universidade Federal do Vale do Sao Francisco; BrasilFil: Costa, Mateus Matiuzzi da. Universidade Federal do Vale do Sao Francisco; BrasilFil: Figueiredo, Henrique César Pereira. Universidade Federal de Minas Gerais; BrasilFil: Portela, Ricardo Dias. Universidade Federal da Bahia; BrasilFil: Castro, Thiago Luiz de Paula. Universidade Federal da Bahia; BrasilFil: Azevedo, Vasco. Universidade Federal de Minas Gerais; BrasilFil: Seyffert, Nubia. Universidade Federal da Bahia; BrasilFil: Meyer, Roberto. Universidade Federal da Bahia; BrasilFrontiers Media2021-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/155309Sá, Maria Conceição Aquino de; Marques Da Silva, Wanderson; Rodrigues, Carla Catarine Santos; Rezende, Cristiana Perdigão; Marchioro, Silvana Beutinger; et al.; Comparative Proteomic Analyses Between Biofilm-Forming and Non-biofilm-Forming Strains of Corynebacterium pseudotuberculosis Isolated From Goats; Frontiers Media; Frontiers in Veterinary Science; 8; 2-2021; 1-102297-1769CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fvets.2021.614011/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fvets.2021.614011info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T11:23:04Zoai:ri.conicet.gov.ar:11336/155309instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 11:23:05.112CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Comparative Proteomic Analyses Between Biofilm-Forming and Non-biofilm-Forming Strains of Corynebacterium pseudotuberculosis Isolated From Goats |
| title |
Comparative Proteomic Analyses Between Biofilm-Forming and Non-biofilm-Forming Strains of Corynebacterium pseudotuberculosis Isolated From Goats |
| spellingShingle |
Comparative Proteomic Analyses Between Biofilm-Forming and Non-biofilm-Forming Strains of Corynebacterium pseudotuberculosis Isolated From Goats Sá, Maria Conceição Aquino de BACTERIAL BIOFILM CASEOUS LYMPHADENITIS PROTEOMICS SMALL RUMINANTS VIRULENCE FACTORS |
| title_short |
Comparative Proteomic Analyses Between Biofilm-Forming and Non-biofilm-Forming Strains of Corynebacterium pseudotuberculosis Isolated From Goats |
| title_full |
Comparative Proteomic Analyses Between Biofilm-Forming and Non-biofilm-Forming Strains of Corynebacterium pseudotuberculosis Isolated From Goats |
| title_fullStr |
Comparative Proteomic Analyses Between Biofilm-Forming and Non-biofilm-Forming Strains of Corynebacterium pseudotuberculosis Isolated From Goats |
| title_full_unstemmed |
Comparative Proteomic Analyses Between Biofilm-Forming and Non-biofilm-Forming Strains of Corynebacterium pseudotuberculosis Isolated From Goats |
| title_sort |
Comparative Proteomic Analyses Between Biofilm-Forming and Non-biofilm-Forming Strains of Corynebacterium pseudotuberculosis Isolated From Goats |
| dc.creator.none.fl_str_mv |
Sá, Maria Conceição Aquino de Marques Da Silva, Wanderson Rodrigues, Carla Catarine Santos Rezende, Cristiana Perdigão Marchioro, Silvana Beutinger Rocha Filho, José Tadeu Raynal Sousa, Thiago de Jesus Oliveira, Helinando Pequeno de Costa, Mateus Matiuzzi da Figueiredo, Henrique César Pereira Portela, Ricardo Dias Castro, Thiago Luiz de Paula Azevedo, Vasco Seyffert, Nubia Meyer, Roberto |
| author |
Sá, Maria Conceição Aquino de |
| author_facet |
Sá, Maria Conceição Aquino de Marques Da Silva, Wanderson Rodrigues, Carla Catarine Santos Rezende, Cristiana Perdigão Marchioro, Silvana Beutinger Rocha Filho, José Tadeu Raynal Sousa, Thiago de Jesus Oliveira, Helinando Pequeno de Costa, Mateus Matiuzzi da Figueiredo, Henrique César Pereira Portela, Ricardo Dias Castro, Thiago Luiz de Paula Azevedo, Vasco Seyffert, Nubia Meyer, Roberto |
| author_role |
author |
| author2 |
Marques Da Silva, Wanderson Rodrigues, Carla Catarine Santos Rezende, Cristiana Perdigão Marchioro, Silvana Beutinger Rocha Filho, José Tadeu Raynal Sousa, Thiago de Jesus Oliveira, Helinando Pequeno de Costa, Mateus Matiuzzi da Figueiredo, Henrique César Pereira Portela, Ricardo Dias Castro, Thiago Luiz de Paula Azevedo, Vasco Seyffert, Nubia Meyer, Roberto |
| author2_role |
author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
BACTERIAL BIOFILM CASEOUS LYMPHADENITIS PROTEOMICS SMALL RUMINANTS VIRULENCE FACTORS |
| topic |
BACTERIAL BIOFILM CASEOUS LYMPHADENITIS PROTEOMICS SMALL RUMINANTS VIRULENCE FACTORS |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
Caseous lymphadenitis (CLA) is a chronic disease that affects small ruminants and causes economic losses in the associated breeding system. The causative agent of CLA is Corynebacterium pseudotuberculosis, a Gram-positive bacterium that exhibits tropism for external and internal lymph nodes and induces abscess formation in the host. Bacterial communities often produce a biofilm matrix that serves various functions, including protection against hostile environmental conditions, antibiotics, and the host immune response. Although biofilm formation has been reported for C. pseudotuberculosis, not all strains demonstrate this property in culture. In this work, we report the first comparative proteomic analysis of one biofilm-forming (CAPJ4) and one biofilm-non-forming strain (CAP3W) of C. pseudotuberculosis isolated from goats. Bacterial whole cell protein extracts were obtained for mass spectrometry analyses. Using LC-MS/MS, our studies reveal three and four proteins exclusively found in the CAPJ4 and CAP3W proteome, respectively. In addition, label-free quantitative analysis identified 40 proteins showing at-least 2-fold higher values in CAPJ4 compared CAP3W proteome Notably, CAPJ4 differentially synthesized the penicillin-binding protein, which participates in the formation of peptidoglycans. CAPJ4 also exhibited upregulation of N-acetylmuramoyl-L-alanine amidase and galactose-1-phosphate uridylyltransferase, which are involved in biofilm formation and exopolysaccharide biosynthesis. Here, we demonstrate that biofilm formation in C. pseudotuberculosis is likely associated with specific proteins, some of which were previously shown to be associated with virulence and biofilm formation in other organisms. Our findings may drive studies related to the bacterial mechanisms involved in the biofilm formation, in addition to providing targets for the treatment of CLA. Fil: Sá, Maria Conceição Aquino de. Universidade Federal da Bahia; Brasil Fil: Marques Da Silva, Wanderson. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Rodrigues, Carla Catarine Santos. Universidade Federal da Bahia; Brasil Fil: Rezende, Cristiana Perdigão. Universidade Federal de Minas Gerais; Brasil Fil: Marchioro, Silvana Beutinger. Universidade Federal da Bahia; Brasil Fil: Rocha Filho, José Tadeu Raynal. Universidade Federal da Bahia; Brasil Fil: Sousa, Thiago de Jesus. Universidade Federal de Minas Gerais; Brasil Fil: Oliveira, Helinando Pequeno de. Universidade Federal do Vale do Sao Francisco; Brasil Fil: Costa, Mateus Matiuzzi da. Universidade Federal do Vale do Sao Francisco; Brasil Fil: Figueiredo, Henrique César Pereira. Universidade Federal de Minas Gerais; Brasil Fil: Portela, Ricardo Dias. Universidade Federal da Bahia; Brasil Fil: Castro, Thiago Luiz de Paula. Universidade Federal da Bahia; Brasil Fil: Azevedo, Vasco. Universidade Federal de Minas Gerais; Brasil Fil: Seyffert, Nubia. Universidade Federal da Bahia; Brasil Fil: Meyer, Roberto. Universidade Federal da Bahia; Brasil |
| description |
Caseous lymphadenitis (CLA) is a chronic disease that affects small ruminants and causes economic losses in the associated breeding system. The causative agent of CLA is Corynebacterium pseudotuberculosis, a Gram-positive bacterium that exhibits tropism for external and internal lymph nodes and induces abscess formation in the host. Bacterial communities often produce a biofilm matrix that serves various functions, including protection against hostile environmental conditions, antibiotics, and the host immune response. Although biofilm formation has been reported for C. pseudotuberculosis, not all strains demonstrate this property in culture. In this work, we report the first comparative proteomic analysis of one biofilm-forming (CAPJ4) and one biofilm-non-forming strain (CAP3W) of C. pseudotuberculosis isolated from goats. Bacterial whole cell protein extracts were obtained for mass spectrometry analyses. Using LC-MS/MS, our studies reveal three and four proteins exclusively found in the CAPJ4 and CAP3W proteome, respectively. In addition, label-free quantitative analysis identified 40 proteins showing at-least 2-fold higher values in CAPJ4 compared CAP3W proteome Notably, CAPJ4 differentially synthesized the penicillin-binding protein, which participates in the formation of peptidoglycans. CAPJ4 also exhibited upregulation of N-acetylmuramoyl-L-alanine amidase and galactose-1-phosphate uridylyltransferase, which are involved in biofilm formation and exopolysaccharide biosynthesis. Here, we demonstrate that biofilm formation in C. pseudotuberculosis is likely associated with specific proteins, some of which were previously shown to be associated with virulence and biofilm formation in other organisms. Our findings may drive studies related to the bacterial mechanisms involved in the biofilm formation, in addition to providing targets for the treatment of CLA. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021-02 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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http://hdl.handle.net/11336/155309 Sá, Maria Conceição Aquino de; Marques Da Silva, Wanderson; Rodrigues, Carla Catarine Santos; Rezende, Cristiana Perdigão; Marchioro, Silvana Beutinger; et al.; Comparative Proteomic Analyses Between Biofilm-Forming and Non-biofilm-Forming Strains of Corynebacterium pseudotuberculosis Isolated From Goats; Frontiers Media; Frontiers in Veterinary Science; 8; 2-2021; 1-10 2297-1769 CONICET Digital CONICET |
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http://hdl.handle.net/11336/155309 |
| identifier_str_mv |
Sá, Maria Conceição Aquino de; Marques Da Silva, Wanderson; Rodrigues, Carla Catarine Santos; Rezende, Cristiana Perdigão; Marchioro, Silvana Beutinger; et al.; Comparative Proteomic Analyses Between Biofilm-Forming and Non-biofilm-Forming Strains of Corynebacterium pseudotuberculosis Isolated From Goats; Frontiers Media; Frontiers in Veterinary Science; 8; 2-2021; 1-10 2297-1769 CONICET Digital CONICET |
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eng |
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eng |
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