Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB)

Autores
Pavan, María Elisa; Robles, Carlos Alejandro; Cairó, Fabián A.; Marcellino, Romanela; Pettinari, María Julia
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Caseous lymphadenitis, caused by Corynebacterium pseudotuberculosis, has a high prevalence in many regions of the world, including Argentina and Brazil. A polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) method for the identification of this microorganism was designed based on the hypervariable region of the polymorphic RNA polymerase b-subunit gene (rpoB). All available Corynebacterium rpoB sequences were analyzed by computer-assisted restriction analysis. The rpoB PCR–RFLP pattern predicted by using endonucleases MseI and StuI clearly differentiated C. pseudotuberculosis from sixty-one other Corynebacterium species. This method was successfully applied to identify twelve wild C. pseudotuberculosis ovine isolates and one caprine isolate. It was also used to differentiate C. pseudotuberculosis from Arcanobacterium pyogenes, an ovine pathogen with similar clinical characteristics. These results indicate that this new molecular method can be used for the reliable identification of the pathogen, essential for the timely detection of infected animals and for epidemiological studies.
Estación Experimental Agropecuaria Bariloche. Área de Producción Animal. Grupo de Salud Animal
Fil: Paván, María Elisa. Biochemiq S.A. Laboratorio de Biología Molecular; Argentina
Fil: Robles, Carlos Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área de Producción Animal. Grupo Salud Animal; Argentina
Fil: Cairó, Fabián A. Biochemiq S.A. Laboratorio de Biología Molecular; Argentina
Fil: Marcellino, Romanela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área de Producción Animal. Grupo Salud Animal; Argentina
FiL: Pettinari, María Julia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fuente
Research in Veterinary Science 92 : 202–206 (2012)
Materia
Corynebacterium
Sanidad Animal
Ovinos
Animal Health
Sheep
Caseous Lymphadenitis
Identification
PCR
Linfoadenitis Caseosa
Corynebacterium pseudotuberculosis
Identificación
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3935
Acceder
id INTADig_a8cf70fa070fcab679bbbc371382f04e
oai_identifier_str oai:localhost:20.500.12123/3935
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB)Pavan, María ElisaRobles, Carlos AlejandroCairó, Fabián A.Marcellino, RomanelaPettinari, María JuliaCorynebacteriumSanidad AnimalOvinosAnimal HealthSheepCaseous LymphadenitisIdentificationPCRLinfoadenitis CaseosaCorynebacterium pseudotuberculosisIdentificaciónCaseous lymphadenitis, caused by Corynebacterium pseudotuberculosis, has a high prevalence in many regions of the world, including Argentina and Brazil. A polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) method for the identification of this microorganism was designed based on the hypervariable region of the polymorphic RNA polymerase b-subunit gene (rpoB). All available Corynebacterium rpoB sequences were analyzed by computer-assisted restriction analysis. The rpoB PCR–RFLP pattern predicted by using endonucleases MseI and StuI clearly differentiated C. pseudotuberculosis from sixty-one other Corynebacterium species. This method was successfully applied to identify twelve wild C. pseudotuberculosis ovine isolates and one caprine isolate. It was also used to differentiate C. pseudotuberculosis from Arcanobacterium pyogenes, an ovine pathogen with similar clinical characteristics. These results indicate that this new molecular method can be used for the reliable identification of the pathogen, essential for the timely detection of infected animals and for epidemiological studies.Estación Experimental Agropecuaria Bariloche. Área de Producción Animal. Grupo de Salud AnimalFil: Paván, María Elisa. Biochemiq S.A. Laboratorio de Biología Molecular; ArgentinaFil: Robles, Carlos Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área de Producción Animal. Grupo Salud Animal; ArgentinaFil: Cairó, Fabián A. Biochemiq S.A. Laboratorio de Biología Molecular; ArgentinaFil: Marcellino, Romanela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área de Producción Animal. Grupo Salud Animal; ArgentinaFiL: Pettinari, María Julia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaElsevier2018-11-21T12:42:40Z2018-11-21T12:42:40Z2012-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/3935https://www.sciencedirect.com/science/article/pii/S00345288110008530034-5288https://doi.org/10.1016/j.rvsc.2011.02.007Research in Veterinary Science 92 : 202–206 (2012)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:30Zoai:localhost:20.500.12123/3935instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:30.833INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB)
title Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB)
spellingShingle Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB)
Pavan, María Elisa
Corynebacterium
Sanidad Animal
Ovinos
Animal Health
Sheep
Caseous Lymphadenitis
Identification
PCR
Linfoadenitis Caseosa
Corynebacterium pseudotuberculosis
Identificación
title_short Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB)
title_full Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB)
title_fullStr Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB)
title_full_unstemmed Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB)
title_sort Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB)
dc.creator.none.fl_str_mv Pavan, María Elisa
Robles, Carlos Alejandro
Cairó, Fabián A.
Marcellino, Romanela
Pettinari, María Julia
author Pavan, María Elisa
author_facet Pavan, María Elisa
Robles, Carlos Alejandro
Cairó, Fabián A.
Marcellino, Romanela
Pettinari, María Julia
author_role author
author2 Robles, Carlos Alejandro
Cairó, Fabián A.
Marcellino, Romanela
Pettinari, María Julia
author2_role author
author
author
author
dc.subject.none.fl_str_mv Corynebacterium
Sanidad Animal
Ovinos
Animal Health
Sheep
Caseous Lymphadenitis
Identification
PCR
Linfoadenitis Caseosa
Corynebacterium pseudotuberculosis
Identificación
topic Corynebacterium
Sanidad Animal
Ovinos
Animal Health
Sheep
Caseous Lymphadenitis
Identification
PCR
Linfoadenitis Caseosa
Corynebacterium pseudotuberculosis
Identificación
dc.description.none.fl_txt_mv Caseous lymphadenitis, caused by Corynebacterium pseudotuberculosis, has a high prevalence in many regions of the world, including Argentina and Brazil. A polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) method for the identification of this microorganism was designed based on the hypervariable region of the polymorphic RNA polymerase b-subunit gene (rpoB). All available Corynebacterium rpoB sequences were analyzed by computer-assisted restriction analysis. The rpoB PCR–RFLP pattern predicted by using endonucleases MseI and StuI clearly differentiated C. pseudotuberculosis from sixty-one other Corynebacterium species. This method was successfully applied to identify twelve wild C. pseudotuberculosis ovine isolates and one caprine isolate. It was also used to differentiate C. pseudotuberculosis from Arcanobacterium pyogenes, an ovine pathogen with similar clinical characteristics. These results indicate that this new molecular method can be used for the reliable identification of the pathogen, essential for the timely detection of infected animals and for epidemiological studies.
Estación Experimental Agropecuaria Bariloche. Área de Producción Animal. Grupo de Salud Animal
Fil: Paván, María Elisa. Biochemiq S.A. Laboratorio de Biología Molecular; Argentina
Fil: Robles, Carlos Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área de Producción Animal. Grupo Salud Animal; Argentina
Fil: Cairó, Fabián A. Biochemiq S.A. Laboratorio de Biología Molecular; Argentina
Fil: Marcellino, Romanela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área de Producción Animal. Grupo Salud Animal; Argentina
FiL: Pettinari, María Julia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
description Caseous lymphadenitis, caused by Corynebacterium pseudotuberculosis, has a high prevalence in many regions of the world, including Argentina and Brazil. A polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) method for the identification of this microorganism was designed based on the hypervariable region of the polymorphic RNA polymerase b-subunit gene (rpoB). All available Corynebacterium rpoB sequences were analyzed by computer-assisted restriction analysis. The rpoB PCR–RFLP pattern predicted by using endonucleases MseI and StuI clearly differentiated C. pseudotuberculosis from sixty-one other Corynebacterium species. This method was successfully applied to identify twelve wild C. pseudotuberculosis ovine isolates and one caprine isolate. It was also used to differentiate C. pseudotuberculosis from Arcanobacterium pyogenes, an ovine pathogen with similar clinical characteristics. These results indicate that this new molecular method can be used for the reliable identification of the pathogen, essential for the timely detection of infected animals and for epidemiological studies.
publishDate 2012
dc.date.none.fl_str_mv 2012-04
2018-11-21T12:42:40Z
2018-11-21T12:42:40Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/3935
https://www.sciencedirect.com/science/article/pii/S0034528811000853
0034-5288
https://doi.org/10.1016/j.rvsc.2011.02.007
url http://hdl.handle.net/20.500.12123/3935
https://www.sciencedirect.com/science/article/pii/S0034528811000853
https://doi.org/10.1016/j.rvsc.2011.02.007
identifier_str_mv 0034-5288
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Research in Veterinary Science 92 : 202–206 (2012)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1844619128534466560
score 12.559606

Publicaciones similares