Skin damage mechanisms related to airborne particulate matter exposure

Autores
Magnani, Natalia Daniela; Muresan, Ximena M.; Belmonte, Giuseppe; Cervellati, Franco; Sticozzi, Claudia; Pecorelli, Alessandra; Miracco, Clelia; Marchini, Timoteo Oscar; Evelson, Pablo Andrés; Valacchi, Giuseppe
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Epidemiological studies suggest a correlation between increased airborne particulate matter (PM) and adverse health effects. The mechanisms of PM-health effects are believed to involve oxidative stress and inflammation. To evaluate the ability of PM promoting skin tissue damage, one of the main organs exposed to outdoor pollutants, we analyzed the effect of concentrated ambient particles (CAPs) in a reconstructed human epidermis (RHE) model. RHE tissues were exposed to 25 or 100 μg/ml CAPs for 24 or 48 h. Data showed that RHE seems to be more susceptible to CAPs-induced toxicity after 48 h exposure than after 24 h. We found a local reactive O2 species (ROS) production increase generated from metals present on the particle, which contributes to lipids oxidation. Furthermore, as a consequence of altered redox status, NFkB nucleus translocation was increase upon CAPs exposure, as well as cyclooxygenase 2 and cytochrome P450 levels, which may be involved in the inflammatory response initiated by PM. CAPs also triggered an apoptotic process in skin. Surprisingly, by transition electron microscopy analysis we showed that CAPs were able to penetrate skin tissues. These findings contribute to the understanding of the cutaneous pathophysiological mechanisms initiated by CAPs exposure, where oxidative stress and inflammation may play predominant roles.
Fil: Magnani, Natalia Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Muresan, Ximena M.. Universita Di Ferrara; Italia
Fil: Belmonte, Giuseppe. Universita Di Ferrara; Italia
Fil: Cervellati, Franco. Universita Di Ferrara; Italia
Fil: Sticozzi, Claudia. Universita Di Ferrara; Italia
Fil: Pecorelli, Alessandra. Universita Di Ferrara; Italia
Fil: Miracco, Clelia. Università Degli Studi Di Siena; Italia
Fil: Marchini, Timoteo Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Evelson, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Valacchi, Giuseppe. Universita Di Ferrara; Italia
Materia
Air Pollution
Cutaneous Tissues
Inflammation
Oxidative Damage
Particulate Matter
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/38814
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/38814
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Skin damage mechanisms related to airborne particulate matter exposureMagnani, Natalia DanielaMuresan, Ximena M.Belmonte, GiuseppeCervellati, FrancoSticozzi, ClaudiaPecorelli, AlessandraMiracco, CleliaMarchini, Timoteo OscarEvelson, Pablo AndrésValacchi, GiuseppeAir PollutionCutaneous TissuesInflammationOxidative DamageParticulate Matterhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Epidemiological studies suggest a correlation between increased airborne particulate matter (PM) and adverse health effects. The mechanisms of PM-health effects are believed to involve oxidative stress and inflammation. To evaluate the ability of PM promoting skin tissue damage, one of the main organs exposed to outdoor pollutants, we analyzed the effect of concentrated ambient particles (CAPs) in a reconstructed human epidermis (RHE) model. RHE tissues were exposed to 25 or 100 μg/ml CAPs for 24 or 48 h. Data showed that RHE seems to be more susceptible to CAPs-induced toxicity after 48 h exposure than after 24 h. We found a local reactive O2 species (ROS) production increase generated from metals present on the particle, which contributes to lipids oxidation. Furthermore, as a consequence of altered redox status, NFkB nucleus translocation was increase upon CAPs exposure, as well as cyclooxygenase 2 and cytochrome P450 levels, which may be involved in the inflammatory response initiated by PM. CAPs also triggered an apoptotic process in skin. Surprisingly, by transition electron microscopy analysis we showed that CAPs were able to penetrate skin tissues. These findings contribute to the understanding of the cutaneous pathophysiological mechanisms initiated by CAPs exposure, where oxidative stress and inflammation may play predominant roles.Fil: Magnani, Natalia Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Muresan, Ximena M.. Universita Di Ferrara; ItaliaFil: Belmonte, Giuseppe. Universita Di Ferrara; ItaliaFil: Cervellati, Franco. Universita Di Ferrara; ItaliaFil: Sticozzi, Claudia. Universita Di Ferrara; ItaliaFil: Pecorelli, Alessandra. Universita Di Ferrara; ItaliaFil: Miracco, Clelia. Università Degli Studi Di Siena; ItaliaFil: Marchini, Timoteo Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Evelson, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Valacchi, Giuseppe. Universita Di Ferrara; ItaliaOxford University Press2016-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/38814Magnani, Natalia Daniela; Muresan, Ximena M.; Belmonte, Giuseppe; Cervellati, Franco; Sticozzi, Claudia; et al.; Skin damage mechanisms related to airborne particulate matter exposure; Oxford University Press; Toxicological Sciences; 149; 1; 1-2016; 227-2361096-6080CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/toxsci/article/149/1/227/2461704info:eu-repo/semantics/altIdentifier/doi/10.1093/toxsci/kfv230info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:06:57Zoai:ri.conicet.gov.ar:11336/38814instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:06:57.605CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Skin damage mechanisms related to airborne particulate matter exposure
title Skin damage mechanisms related to airborne particulate matter exposure
spellingShingle Skin damage mechanisms related to airborne particulate matter exposure
Magnani, Natalia Daniela
Air Pollution
Cutaneous Tissues
Inflammation
Oxidative Damage
Particulate Matter
title_short Skin damage mechanisms related to airborne particulate matter exposure
title_full Skin damage mechanisms related to airborne particulate matter exposure
title_fullStr Skin damage mechanisms related to airborne particulate matter exposure
title_full_unstemmed Skin damage mechanisms related to airborne particulate matter exposure
title_sort Skin damage mechanisms related to airborne particulate matter exposure
dc.creator.none.fl_str_mv Magnani, Natalia Daniela
Muresan, Ximena M.
Belmonte, Giuseppe
Cervellati, Franco
Sticozzi, Claudia
Pecorelli, Alessandra
Miracco, Clelia
Marchini, Timoteo Oscar
Evelson, Pablo Andrés
Valacchi, Giuseppe
author Magnani, Natalia Daniela
author_facet Magnani, Natalia Daniela
Muresan, Ximena M.
Belmonte, Giuseppe
Cervellati, Franco
Sticozzi, Claudia
Pecorelli, Alessandra
Miracco, Clelia
Marchini, Timoteo Oscar
Evelson, Pablo Andrés
Valacchi, Giuseppe
author_role author
author2 Muresan, Ximena M.
Belmonte, Giuseppe
Cervellati, Franco
Sticozzi, Claudia
Pecorelli, Alessandra
Miracco, Clelia
Marchini, Timoteo Oscar
Evelson, Pablo Andrés
Valacchi, Giuseppe
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Air Pollution
Cutaneous Tissues
Inflammation
Oxidative Damage
Particulate Matter
topic Air Pollution
Cutaneous Tissues
Inflammation
Oxidative Damage
Particulate Matter
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Epidemiological studies suggest a correlation between increased airborne particulate matter (PM) and adverse health effects. The mechanisms of PM-health effects are believed to involve oxidative stress and inflammation. To evaluate the ability of PM promoting skin tissue damage, one of the main organs exposed to outdoor pollutants, we analyzed the effect of concentrated ambient particles (CAPs) in a reconstructed human epidermis (RHE) model. RHE tissues were exposed to 25 or 100 μg/ml CAPs for 24 or 48 h. Data showed that RHE seems to be more susceptible to CAPs-induced toxicity after 48 h exposure than after 24 h. We found a local reactive O2 species (ROS) production increase generated from metals present on the particle, which contributes to lipids oxidation. Furthermore, as a consequence of altered redox status, NFkB nucleus translocation was increase upon CAPs exposure, as well as cyclooxygenase 2 and cytochrome P450 levels, which may be involved in the inflammatory response initiated by PM. CAPs also triggered an apoptotic process in skin. Surprisingly, by transition electron microscopy analysis we showed that CAPs were able to penetrate skin tissues. These findings contribute to the understanding of the cutaneous pathophysiological mechanisms initiated by CAPs exposure, where oxidative stress and inflammation may play predominant roles.
Fil: Magnani, Natalia Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Muresan, Ximena M.. Universita Di Ferrara; Italia
Fil: Belmonte, Giuseppe. Universita Di Ferrara; Italia
Fil: Cervellati, Franco. Universita Di Ferrara; Italia
Fil: Sticozzi, Claudia. Universita Di Ferrara; Italia
Fil: Pecorelli, Alessandra. Universita Di Ferrara; Italia
Fil: Miracco, Clelia. Università Degli Studi Di Siena; Italia
Fil: Marchini, Timoteo Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Evelson, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Valacchi, Giuseppe. Universita Di Ferrara; Italia
description Epidemiological studies suggest a correlation between increased airborne particulate matter (PM) and adverse health effects. The mechanisms of PM-health effects are believed to involve oxidative stress and inflammation. To evaluate the ability of PM promoting skin tissue damage, one of the main organs exposed to outdoor pollutants, we analyzed the effect of concentrated ambient particles (CAPs) in a reconstructed human epidermis (RHE) model. RHE tissues were exposed to 25 or 100 μg/ml CAPs for 24 or 48 h. Data showed that RHE seems to be more susceptible to CAPs-induced toxicity after 48 h exposure than after 24 h. We found a local reactive O2 species (ROS) production increase generated from metals present on the particle, which contributes to lipids oxidation. Furthermore, as a consequence of altered redox status, NFkB nucleus translocation was increase upon CAPs exposure, as well as cyclooxygenase 2 and cytochrome P450 levels, which may be involved in the inflammatory response initiated by PM. CAPs also triggered an apoptotic process in skin. Surprisingly, by transition electron microscopy analysis we showed that CAPs were able to penetrate skin tissues. These findings contribute to the understanding of the cutaneous pathophysiological mechanisms initiated by CAPs exposure, where oxidative stress and inflammation may play predominant roles.
publishDate 2016
dc.date.none.fl_str_mv 2016-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/38814
Magnani, Natalia Daniela; Muresan, Ximena M.; Belmonte, Giuseppe; Cervellati, Franco; Sticozzi, Claudia; et al.; Skin damage mechanisms related to airborne particulate matter exposure; Oxford University Press; Toxicological Sciences; 149; 1; 1-2016; 227-236
1096-6080
CONICET Digital
CONICET
url http://hdl.handle.net/11336/38814
identifier_str_mv Magnani, Natalia Daniela; Muresan, Ximena M.; Belmonte, Giuseppe; Cervellati, Franco; Sticozzi, Claudia; et al.; Skin damage mechanisms related to airborne particulate matter exposure; Oxford University Press; Toxicological Sciences; 149; 1; 1-2016; 227-236
1096-6080
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/toxsci/article/149/1/227/2461704
info:eu-repo/semantics/altIdentifier/doi/10.1093/toxsci/kfv230
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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