Aeromonas hydrophila strains as biocatalysts for transglycosylation

Autores
Nóbile, Matías Leonardo; Terreni, Marco; Lewkowicz, Elizabeth Sandra; Iribarren, Adolfo Marcelo
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Microbial transglycosylation is useful as a green alternative in the preparation of purine nucleosides and analogues, especially for those that display pharmacological activities. In a search for new transglycosylation biocatalysts, two Aeromonas hydrophila strains were selected. The substrate specificity of both micro-organisms was studied and, as a result, several nucleoside analogues have been prepared. Among them, ribavirin, a broad spectrum antiviral, and the well-known anti HIV didanosine, were prepared, in 77 and 62% yield using A. hydrophila CECT 4226 and A. hydrophila CECT 4221, respectively. In order to scale-up the processes, the reaction conditions, product purification and biocatalyst preparation were analyzed and optimized.
Fil: Nóbile, Matías Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; Argentina
Fil: Terreni, Marco. Universita degli Studi di Pavia; Italia
Fil: Lewkowicz, Elizabeth Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; Argentina
Fil: Iribarren, Adolfo Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad Nacional de Quilmes; Argentina
Materia
AEROMONAS HYDROPHILA
DIDANOSINE
MICROBIAL TRANSGLYCOSYLATION
RIBAVIRIN
WHOLE CELL BIOCATALYSTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/190810
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Aeromonas hydrophila strains as biocatalysts for transglycosylationNóbile, Matías LeonardoTerreni, MarcoLewkowicz, Elizabeth SandraIribarren, Adolfo MarceloAEROMONAS HYDROPHILADIDANOSINEMICROBIAL TRANSGLYCOSYLATIONRIBAVIRINWHOLE CELL BIOCATALYSTShttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Microbial transglycosylation is useful as a green alternative in the preparation of purine nucleosides and analogues, especially for those that display pharmacological activities. In a search for new transglycosylation biocatalysts, two Aeromonas hydrophila strains were selected. The substrate specificity of both micro-organisms was studied and, as a result, several nucleoside analogues have been prepared. Among them, ribavirin, a broad spectrum antiviral, and the well-known anti HIV didanosine, were prepared, in 77 and 62% yield using A. hydrophila CECT 4226 and A. hydrophila CECT 4221, respectively. In order to scale-up the processes, the reaction conditions, product purification and biocatalyst preparation were analyzed and optimized.Fil: Nóbile, Matías Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; ArgentinaFil: Terreni, Marco. Universita degli Studi di Pavia; ItaliaFil: Lewkowicz, Elizabeth Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; ArgentinaFil: Iribarren, Adolfo Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad Nacional de Quilmes; ArgentinaTaylor & Francis Ltd2010-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/190810Nóbile, Matías Leonardo; Terreni, Marco; Lewkowicz, Elizabeth Sandra; Iribarren, Adolfo Marcelo; Aeromonas hydrophila strains as biocatalysts for transglycosylation; Taylor & Francis Ltd; Biocatalysis and Biotransformation; 28; 5-6; 12-2010; 395-4021024-2422CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.3109/10242422.2010.538949info:eu-repo/semantics/altIdentifier/doi/10.3109/10242422.2010.538949info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:26:46Zoai:ri.conicet.gov.ar:11336/190810instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:26:46.533CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Aeromonas hydrophila strains as biocatalysts for transglycosylation
title Aeromonas hydrophila strains as biocatalysts for transglycosylation
spellingShingle Aeromonas hydrophila strains as biocatalysts for transglycosylation
Nóbile, Matías Leonardo
AEROMONAS HYDROPHILA
DIDANOSINE
MICROBIAL TRANSGLYCOSYLATION
RIBAVIRIN
WHOLE CELL BIOCATALYSTS
title_short Aeromonas hydrophila strains as biocatalysts for transglycosylation
title_full Aeromonas hydrophila strains as biocatalysts for transglycosylation
title_fullStr Aeromonas hydrophila strains as biocatalysts for transglycosylation
title_full_unstemmed Aeromonas hydrophila strains as biocatalysts for transglycosylation
title_sort Aeromonas hydrophila strains as biocatalysts for transglycosylation
dc.creator.none.fl_str_mv Nóbile, Matías Leonardo
Terreni, Marco
Lewkowicz, Elizabeth Sandra
Iribarren, Adolfo Marcelo
author Nóbile, Matías Leonardo
author_facet Nóbile, Matías Leonardo
Terreni, Marco
Lewkowicz, Elizabeth Sandra
Iribarren, Adolfo Marcelo
author_role author
author2 Terreni, Marco
Lewkowicz, Elizabeth Sandra
Iribarren, Adolfo Marcelo
author2_role author
author
author
dc.subject.none.fl_str_mv AEROMONAS HYDROPHILA
DIDANOSINE
MICROBIAL TRANSGLYCOSYLATION
RIBAVIRIN
WHOLE CELL BIOCATALYSTS
topic AEROMONAS HYDROPHILA
DIDANOSINE
MICROBIAL TRANSGLYCOSYLATION
RIBAVIRIN
WHOLE CELL BIOCATALYSTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Microbial transglycosylation is useful as a green alternative in the preparation of purine nucleosides and analogues, especially for those that display pharmacological activities. In a search for new transglycosylation biocatalysts, two Aeromonas hydrophila strains were selected. The substrate specificity of both micro-organisms was studied and, as a result, several nucleoside analogues have been prepared. Among them, ribavirin, a broad spectrum antiviral, and the well-known anti HIV didanosine, were prepared, in 77 and 62% yield using A. hydrophila CECT 4226 and A. hydrophila CECT 4221, respectively. In order to scale-up the processes, the reaction conditions, product purification and biocatalyst preparation were analyzed and optimized.
Fil: Nóbile, Matías Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; Argentina
Fil: Terreni, Marco. Universita degli Studi di Pavia; Italia
Fil: Lewkowicz, Elizabeth Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; Argentina
Fil: Iribarren, Adolfo Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad Nacional de Quilmes; Argentina
description Microbial transglycosylation is useful as a green alternative in the preparation of purine nucleosides and analogues, especially for those that display pharmacological activities. In a search for new transglycosylation biocatalysts, two Aeromonas hydrophila strains were selected. The substrate specificity of both micro-organisms was studied and, as a result, several nucleoside analogues have been prepared. Among them, ribavirin, a broad spectrum antiviral, and the well-known anti HIV didanosine, were prepared, in 77 and 62% yield using A. hydrophila CECT 4226 and A. hydrophila CECT 4221, respectively. In order to scale-up the processes, the reaction conditions, product purification and biocatalyst preparation were analyzed and optimized.
publishDate 2010
dc.date.none.fl_str_mv 2010-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/190810
Nóbile, Matías Leonardo; Terreni, Marco; Lewkowicz, Elizabeth Sandra; Iribarren, Adolfo Marcelo; Aeromonas hydrophila strains as biocatalysts for transglycosylation; Taylor & Francis Ltd; Biocatalysis and Biotransformation; 28; 5-6; 12-2010; 395-402
1024-2422
CONICET Digital
CONICET
url http://hdl.handle.net/11336/190810
identifier_str_mv Nóbile, Matías Leonardo; Terreni, Marco; Lewkowicz, Elizabeth Sandra; Iribarren, Adolfo Marcelo; Aeromonas hydrophila strains as biocatalysts for transglycosylation; Taylor & Francis Ltd; Biocatalysis and Biotransformation; 28; 5-6; 12-2010; 395-402
1024-2422
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.3109/10242422.2010.538949
info:eu-repo/semantics/altIdentifier/doi/10.3109/10242422.2010.538949
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis Ltd
publisher.none.fl_str_mv Taylor & Francis Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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