Effect of quorum sensing molecules and natamycin on biofilms of Candida tropicalis and other yeasts isolated from industrial juice filtration membranes

Autores
Agustín, María del Rosario; Viceconte, Fátima Regina; Vela Gurovic, Maria Soledad; Costantino, Andrea Rosana; Brugnoni, Lorena Inés
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aims: Cells limit the cell number of dense biofilms by releasing self-inhibitory molecules. Here, we aim to assess the effectiveness of yeast quorum sensing (QS) molecules and the antifungal agent natamycin against yeast biofilms of strains commonly isolated from fruit juice ultrafiltration membranes.Methods and results.Yeast QS molecules such as tyrosol, 2-phenylethanol and farnesol were detected by solvent extraction and HS SPME GC-MS in C. tropicalis cultures. The effect of quorum sensing (QS) molecules on mono and multi species biofilms formed by Rhodotorula mucilaginosa, Candida tropicalis, Candida krusei and Candida kefyr was evaluated by plate count and epifluorescence microscopy.Farnesol caused a decrease in cell number and disrupted mono and multispecies yeast biofilms during adhesion (0.6 mmol l-1). 2-phenyl ethanol 1.2 mmol l-1 stimulated biofilm density and increased cell number in both mono and multispecies biofilms, while tyrosol did not show effects when tested against C. tropicalis biofilms (0.05-1.2 mmol l-1). Natamycin caused a strong decrease on cell number and disruption of biofilm structure in C. tropicalis biofilms at high concentrations (0.3 to 1.2 mmol l-1). The combination of farnesol 0.6 mmol l-1 and natamycin at 0.01 mmol l-1, the maximum concentration of natamycin accepted for direct addition into fruit juices, effectively reduced cell counts and disrupted the structure of C. tropicalis biofilms.ConclusionFarnesol 0.6 mmol l-1 significantly increased the inhibition exerted by natamycin 0.01 mmol l-1 (¡­5 ppm) reducing biofilm development from juice on stainless steel surfaces. Significance and impact of the studyThese results support the use of QS molecules as biofilm inhibitors in beverages and would certainly inspire the design of novel preservative and cleaning products for the food industry based on combinatory approaches.
Fil: Agustín, María del Rosario. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Viceconte, Fátima Regina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Vela Gurovic, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Costantino, Andrea Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Brugnoni, Lorena Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Materia
QUORUM SENSING
BIOFILM
JUICE
FARNESOL
CANDIDA SPP.
NATAMYCIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/108262
Acceder
id CONICETDig_81dde1221619a284e37251bfc99a7cb8
oai_identifier_str oai:ri.conicet.gov.ar:11336/108262
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of quorum sensing molecules and natamycin on biofilms of Candida tropicalis and other yeasts isolated from industrial juice filtration membranesAgustín, María del RosarioViceconte, Fátima ReginaVela Gurovic, Maria SoledadCostantino, Andrea RosanaBrugnoni, Lorena InésQUORUM SENSINGBIOFILMJUICEFARNESOLCANDIDA SPP.NATAMYCINhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Aims: Cells limit the cell number of dense biofilms by releasing self-inhibitory molecules. Here, we aim to assess the effectiveness of yeast quorum sensing (QS) molecules and the antifungal agent natamycin against yeast biofilms of strains commonly isolated from fruit juice ultrafiltration membranes.Methods and results.Yeast QS molecules such as tyrosol, 2-phenylethanol and farnesol were detected by solvent extraction and HS SPME GC-MS in C. tropicalis cultures. The effect of quorum sensing (QS) molecules on mono and multi species biofilms formed by Rhodotorula mucilaginosa, Candida tropicalis, Candida krusei and Candida kefyr was evaluated by plate count and epifluorescence microscopy.Farnesol caused a decrease in cell number and disrupted mono and multispecies yeast biofilms during adhesion (0.6 mmol l-1). 2-phenyl ethanol 1.2 mmol l-1 stimulated biofilm density and increased cell number in both mono and multispecies biofilms, while tyrosol did not show effects when tested against C. tropicalis biofilms (0.05-1.2 mmol l-1). Natamycin caused a strong decrease on cell number and disruption of biofilm structure in C. tropicalis biofilms at high concentrations (0.3 to 1.2 mmol l-1). The combination of farnesol 0.6 mmol l-1 and natamycin at 0.01 mmol l-1, the maximum concentration of natamycin accepted for direct addition into fruit juices, effectively reduced cell counts and disrupted the structure of C. tropicalis biofilms.ConclusionFarnesol 0.6 mmol l-1 significantly increased the inhibition exerted by natamycin 0.01 mmol l-1 (¡­5 ppm) reducing biofilm development from juice on stainless steel surfaces. Significance and impact of the studyThese results support the use of QS molecules as biofilm inhibitors in beverages and would certainly inspire the design of novel preservative and cleaning products for the food industry based on combinatory approaches.Fil: Agustín, María del Rosario. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Viceconte, Fátima Regina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Vela Gurovic, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Costantino, Andrea Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Brugnoni, Lorena Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaWiley Blackwell Publishing, Inc2019-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/108262Agustín, María del Rosario; Viceconte, Fátima Regina; Vela Gurovic, Maria Soledad; Costantino, Andrea Rosana; Brugnoni, Lorena Inés; Effect of quorum sensing molecules and natamycin on biofilms of Candida tropicalis and other yeasts isolated from industrial juice filtration membranes; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 126; 6; 4-2019; 1808-18201364-5072CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sfamjournals.onlinelibrary.wiley.com/doi/abs/10.1111/jam.14248info:eu-repo/semantics/altIdentifier/doi/10.1111/jam.14248info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:56:42Zoai:ri.conicet.gov.ar:11336/108262instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:56:42.586CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of quorum sensing molecules and natamycin on biofilms of Candida tropicalis and other yeasts isolated from industrial juice filtration membranes
title Effect of quorum sensing molecules and natamycin on biofilms of Candida tropicalis and other yeasts isolated from industrial juice filtration membranes
spellingShingle Effect of quorum sensing molecules and natamycin on biofilms of Candida tropicalis and other yeasts isolated from industrial juice filtration membranes
Agustín, María del Rosario
QUORUM SENSING
BIOFILM
JUICE
FARNESOL
CANDIDA SPP.
NATAMYCIN
title_short Effect of quorum sensing molecules and natamycin on biofilms of Candida tropicalis and other yeasts isolated from industrial juice filtration membranes
title_full Effect of quorum sensing molecules and natamycin on biofilms of Candida tropicalis and other yeasts isolated from industrial juice filtration membranes
title_fullStr Effect of quorum sensing molecules and natamycin on biofilms of Candida tropicalis and other yeasts isolated from industrial juice filtration membranes
title_full_unstemmed Effect of quorum sensing molecules and natamycin on biofilms of Candida tropicalis and other yeasts isolated from industrial juice filtration membranes
title_sort Effect of quorum sensing molecules and natamycin on biofilms of Candida tropicalis and other yeasts isolated from industrial juice filtration membranes
dc.creator.none.fl_str_mv Agustín, María del Rosario
Viceconte, Fátima Regina
Vela Gurovic, Maria Soledad
Costantino, Andrea Rosana
Brugnoni, Lorena Inés
author Agustín, María del Rosario
author_facet Agustín, María del Rosario
Viceconte, Fátima Regina
Vela Gurovic, Maria Soledad
Costantino, Andrea Rosana
Brugnoni, Lorena Inés
author_role author
author2 Viceconte, Fátima Regina
Vela Gurovic, Maria Soledad
Costantino, Andrea Rosana
Brugnoni, Lorena Inés
author2_role author
author
author
author
dc.subject.none.fl_str_mv QUORUM SENSING
BIOFILM
JUICE
FARNESOL
CANDIDA SPP.
NATAMYCIN
topic QUORUM SENSING
BIOFILM
JUICE
FARNESOL
CANDIDA SPP.
NATAMYCIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Aims: Cells limit the cell number of dense biofilms by releasing self-inhibitory molecules. Here, we aim to assess the effectiveness of yeast quorum sensing (QS) molecules and the antifungal agent natamycin against yeast biofilms of strains commonly isolated from fruit juice ultrafiltration membranes.Methods and results.Yeast QS molecules such as tyrosol, 2-phenylethanol and farnesol were detected by solvent extraction and HS SPME GC-MS in C. tropicalis cultures. The effect of quorum sensing (QS) molecules on mono and multi species biofilms formed by Rhodotorula mucilaginosa, Candida tropicalis, Candida krusei and Candida kefyr was evaluated by plate count and epifluorescence microscopy.Farnesol caused a decrease in cell number and disrupted mono and multispecies yeast biofilms during adhesion (0.6 mmol l-1). 2-phenyl ethanol 1.2 mmol l-1 stimulated biofilm density and increased cell number in both mono and multispecies biofilms, while tyrosol did not show effects when tested against C. tropicalis biofilms (0.05-1.2 mmol l-1). Natamycin caused a strong decrease on cell number and disruption of biofilm structure in C. tropicalis biofilms at high concentrations (0.3 to 1.2 mmol l-1). The combination of farnesol 0.6 mmol l-1 and natamycin at 0.01 mmol l-1, the maximum concentration of natamycin accepted for direct addition into fruit juices, effectively reduced cell counts and disrupted the structure of C. tropicalis biofilms.ConclusionFarnesol 0.6 mmol l-1 significantly increased the inhibition exerted by natamycin 0.01 mmol l-1 (¡­5 ppm) reducing biofilm development from juice on stainless steel surfaces. Significance and impact of the studyThese results support the use of QS molecules as biofilm inhibitors in beverages and would certainly inspire the design of novel preservative and cleaning products for the food industry based on combinatory approaches.
Fil: Agustín, María del Rosario. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Viceconte, Fátima Regina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Vela Gurovic, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Costantino, Andrea Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Brugnoni, Lorena Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
description Aims: Cells limit the cell number of dense biofilms by releasing self-inhibitory molecules. Here, we aim to assess the effectiveness of yeast quorum sensing (QS) molecules and the antifungal agent natamycin against yeast biofilms of strains commonly isolated from fruit juice ultrafiltration membranes.Methods and results.Yeast QS molecules such as tyrosol, 2-phenylethanol and farnesol were detected by solvent extraction and HS SPME GC-MS in C. tropicalis cultures. The effect of quorum sensing (QS) molecules on mono and multi species biofilms formed by Rhodotorula mucilaginosa, Candida tropicalis, Candida krusei and Candida kefyr was evaluated by plate count and epifluorescence microscopy.Farnesol caused a decrease in cell number and disrupted mono and multispecies yeast biofilms during adhesion (0.6 mmol l-1). 2-phenyl ethanol 1.2 mmol l-1 stimulated biofilm density and increased cell number in both mono and multispecies biofilms, while tyrosol did not show effects when tested against C. tropicalis biofilms (0.05-1.2 mmol l-1). Natamycin caused a strong decrease on cell number and disruption of biofilm structure in C. tropicalis biofilms at high concentrations (0.3 to 1.2 mmol l-1). The combination of farnesol 0.6 mmol l-1 and natamycin at 0.01 mmol l-1, the maximum concentration of natamycin accepted for direct addition into fruit juices, effectively reduced cell counts and disrupted the structure of C. tropicalis biofilms.ConclusionFarnesol 0.6 mmol l-1 significantly increased the inhibition exerted by natamycin 0.01 mmol l-1 (¡­5 ppm) reducing biofilm development from juice on stainless steel surfaces. Significance and impact of the studyThese results support the use of QS molecules as biofilm inhibitors in beverages and would certainly inspire the design of novel preservative and cleaning products for the food industry based on combinatory approaches.
publishDate 2019
dc.date.none.fl_str_mv 2019-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/108262
Agustín, María del Rosario; Viceconte, Fátima Regina; Vela Gurovic, Maria Soledad; Costantino, Andrea Rosana; Brugnoni, Lorena Inés; Effect of quorum sensing molecules and natamycin on biofilms of Candida tropicalis and other yeasts isolated from industrial juice filtration membranes; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 126; 6; 4-2019; 1808-1820
1364-5072
CONICET Digital
CONICET
url http://hdl.handle.net/11336/108262
identifier_str_mv Agustín, María del Rosario; Viceconte, Fátima Regina; Vela Gurovic, Maria Soledad; Costantino, Andrea Rosana; Brugnoni, Lorena Inés; Effect of quorum sensing molecules and natamycin on biofilms of Candida tropicalis and other yeasts isolated from industrial juice filtration membranes; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 126; 6; 4-2019; 1808-1820
1364-5072
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://sfamjournals.onlinelibrary.wiley.com/doi/abs/10.1111/jam.14248
info:eu-repo/semantics/altIdentifier/doi/10.1111/jam.14248
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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