Relation between light absorption measured by the quantitative filter technique and attenuation of Chlorella fusca cultures of different cell densities: application to estimate the...

Autores
Jerez, Celia G.; García, Carolina Belén; Rearte, Tomás Agustín; López Figueroa, Félix
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In order to estimate microalgal carbon assimilation or production of Chlorella fusca cultures based on electron transport rate (ETR) as in vivo chlorophyll a fluorescence, it is necessary to determine the photosynthetic yield and the absorbed quanta by measuring the incident irradiance and the fraction of absorbed light, i.e., absorptance or absorption coefficient in the photosynthetic active radiation (PAR) region of the spectra. Due to difficulties associated with the determination of light absorption, ETR is commonly expressed as relative units (rETR) although this is not a good estimator of the photosynthetic production since photobiological responses depend on the absorbed light. The quantitative filter technique (QFT) is commonly used to measure the absorbed quanta of cells retained on a filter (AbQf) as estimator of the absorbed quanta of cell suspensions (AbQs) determined by using integrating spheres. In this study, light attenuation of thin-layer cell suspensions is determined by using a measuring system designed to reduce the scattering. The light attenuation is related to the absorptance as the fraction of absorbed light by both indoor and outdoor C. fusca cultures of different cell densities. A linear relation between AbQf and AbQs (R2 = 0.9902, p < 0.01) was observed, AbQf = 1.98 × AbQs, being 1.98 an amplification factor to convert AbQs values into AbQf ones. On the other hand, depending on the culture system, the convenience of the use of the absorptance, light absorption or specific light absorption coefficient expressed per area (thin-layer cascade or flat panel cultivators), volume (cylindrical and tubular photobioreactors), or chlorophyll units (any type of cultivation system) is discussed. The procedure for the measurement of light absorption presented in this study for C. fusca could be applied in other phytoplankton groups. The absorbed quanta as determined in this study can be used to express absolute ETR instead of relative ETR, since the first one provides much more relevant photobiological information of microalgae culture systems.
Fil: Jerez, Celia G.. Universidad de Malaga. Facultad de Ciencias. Departamento de Ecología y Geología; España
Fil: García, Carolina Belén. Universidad Nacional de Cuyo. Instituto Multidisciplinario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina
Fil: Rearte, Tomás Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Recursos Naturales y Ambiente. Cátedra de Química Analítica; Argentina
Fil: López Figueroa, Félix. Universidad de Malaga. Facultad de Ciencias. Departamento de Ecología y Geología; España
Materia
Absorptance
Chlorella Fusca
Chlorophyceae
Electron Transport Rate
In Vivo Absorption Coefficient
Light Attenuation Coefficient
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/69336
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/69336
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Relation between light absorption measured by the quantitative filter technique and attenuation of Chlorella fusca cultures of different cell densities: application to estimate the absolute electron transport rate (ETR)Jerez, Celia G.García, Carolina BelénRearte, Tomás AgustínLópez Figueroa, FélixAbsorptanceChlorella FuscaChlorophyceaeElectron Transport RateIn Vivo Absorption CoefficientLight Attenuation Coefficienthttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In order to estimate microalgal carbon assimilation or production of Chlorella fusca cultures based on electron transport rate (ETR) as in vivo chlorophyll a fluorescence, it is necessary to determine the photosynthetic yield and the absorbed quanta by measuring the incident irradiance and the fraction of absorbed light, i.e., absorptance or absorption coefficient in the photosynthetic active radiation (PAR) region of the spectra. Due to difficulties associated with the determination of light absorption, ETR is commonly expressed as relative units (rETR) although this is not a good estimator of the photosynthetic production since photobiological responses depend on the absorbed light. The quantitative filter technique (QFT) is commonly used to measure the absorbed quanta of cells retained on a filter (AbQf) as estimator of the absorbed quanta of cell suspensions (AbQs) determined by using integrating spheres. In this study, light attenuation of thin-layer cell suspensions is determined by using a measuring system designed to reduce the scattering. The light attenuation is related to the absorptance as the fraction of absorbed light by both indoor and outdoor C. fusca cultures of different cell densities. A linear relation between AbQf and AbQs (R2 = 0.9902, p < 0.01) was observed, AbQf = 1.98 × AbQs, being 1.98 an amplification factor to convert AbQs values into AbQf ones. On the other hand, depending on the culture system, the convenience of the use of the absorptance, light absorption or specific light absorption coefficient expressed per area (thin-layer cascade or flat panel cultivators), volume (cylindrical and tubular photobioreactors), or chlorophyll units (any type of cultivation system) is discussed. The procedure for the measurement of light absorption presented in this study for C. fusca could be applied in other phytoplankton groups. The absorbed quanta as determined in this study can be used to express absolute ETR instead of relative ETR, since the first one provides much more relevant photobiological information of microalgae culture systems.Fil: Jerez, Celia G.. Universidad de Malaga. Facultad de Ciencias. Departamento de Ecología y Geología; EspañaFil: García, Carolina Belén. Universidad Nacional de Cuyo. Instituto Multidisciplinario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; ArgentinaFil: Rearte, Tomás Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Recursos Naturales y Ambiente. Cátedra de Química Analítica; ArgentinaFil: López Figueroa, Félix. Universidad de Malaga. Facultad de Ciencias. Departamento de Ecología y Geología; EspañaSpringer2016-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/69336Jerez, Celia G.; García, Carolina Belén; Rearte, Tomás Agustín; López Figueroa, Félix; Relation between light absorption measured by the quantitative filter technique and attenuation of Chlorella fusca cultures of different cell densities: application to estimate the absolute electron transport rate (ETR); Springer; Journal of Applied Phycology; 28; 3; 6-2016; 1635-16480921-8971CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s10811-015-0685-3info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s10811-015-0685-3info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-03T08:50:17Zoai:ri.conicet.gov.ar:11336/69336instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-03 08:50:17.474CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Relation between light absorption measured by the quantitative filter technique and attenuation of Chlorella fusca cultures of different cell densities: application to estimate the absolute electron transport rate (ETR)
title Relation between light absorption measured by the quantitative filter technique and attenuation of Chlorella fusca cultures of different cell densities: application to estimate the absolute electron transport rate (ETR)
spellingShingle Relation between light absorption measured by the quantitative filter technique and attenuation of Chlorella fusca cultures of different cell densities: application to estimate the absolute electron transport rate (ETR)
Jerez, Celia G.
Absorptance
Chlorella Fusca
Chlorophyceae
Electron Transport Rate
In Vivo Absorption Coefficient
Light Attenuation Coefficient
title_short Relation between light absorption measured by the quantitative filter technique and attenuation of Chlorella fusca cultures of different cell densities: application to estimate the absolute electron transport rate (ETR)
title_full Relation between light absorption measured by the quantitative filter technique and attenuation of Chlorella fusca cultures of different cell densities: application to estimate the absolute electron transport rate (ETR)
title_fullStr Relation between light absorption measured by the quantitative filter technique and attenuation of Chlorella fusca cultures of different cell densities: application to estimate the absolute electron transport rate (ETR)
title_full_unstemmed Relation between light absorption measured by the quantitative filter technique and attenuation of Chlorella fusca cultures of different cell densities: application to estimate the absolute electron transport rate (ETR)
title_sort Relation between light absorption measured by the quantitative filter technique and attenuation of Chlorella fusca cultures of different cell densities: application to estimate the absolute electron transport rate (ETR)
dc.creator.none.fl_str_mv Jerez, Celia G.
García, Carolina Belén
Rearte, Tomás Agustín
López Figueroa, Félix
author Jerez, Celia G.
author_facet Jerez, Celia G.
García, Carolina Belén
Rearte, Tomás Agustín
López Figueroa, Félix
author_role author
author2 García, Carolina Belén
Rearte, Tomás Agustín
López Figueroa, Félix
author2_role author
author
author
dc.subject.none.fl_str_mv Absorptance
Chlorella Fusca
Chlorophyceae
Electron Transport Rate
In Vivo Absorption Coefficient
Light Attenuation Coefficient
topic Absorptance
Chlorella Fusca
Chlorophyceae
Electron Transport Rate
In Vivo Absorption Coefficient
Light Attenuation Coefficient
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In order to estimate microalgal carbon assimilation or production of Chlorella fusca cultures based on electron transport rate (ETR) as in vivo chlorophyll a fluorescence, it is necessary to determine the photosynthetic yield and the absorbed quanta by measuring the incident irradiance and the fraction of absorbed light, i.e., absorptance or absorption coefficient in the photosynthetic active radiation (PAR) region of the spectra. Due to difficulties associated with the determination of light absorption, ETR is commonly expressed as relative units (rETR) although this is not a good estimator of the photosynthetic production since photobiological responses depend on the absorbed light. The quantitative filter technique (QFT) is commonly used to measure the absorbed quanta of cells retained on a filter (AbQf) as estimator of the absorbed quanta of cell suspensions (AbQs) determined by using integrating spheres. In this study, light attenuation of thin-layer cell suspensions is determined by using a measuring system designed to reduce the scattering. The light attenuation is related to the absorptance as the fraction of absorbed light by both indoor and outdoor C. fusca cultures of different cell densities. A linear relation between AbQf and AbQs (R2 = 0.9902, p < 0.01) was observed, AbQf = 1.98 × AbQs, being 1.98 an amplification factor to convert AbQs values into AbQf ones. On the other hand, depending on the culture system, the convenience of the use of the absorptance, light absorption or specific light absorption coefficient expressed per area (thin-layer cascade or flat panel cultivators), volume (cylindrical and tubular photobioreactors), or chlorophyll units (any type of cultivation system) is discussed. The procedure for the measurement of light absorption presented in this study for C. fusca could be applied in other phytoplankton groups. The absorbed quanta as determined in this study can be used to express absolute ETR instead of relative ETR, since the first one provides much more relevant photobiological information of microalgae culture systems.
Fil: Jerez, Celia G.. Universidad de Malaga. Facultad de Ciencias. Departamento de Ecología y Geología; España
Fil: García, Carolina Belén. Universidad Nacional de Cuyo. Instituto Multidisciplinario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina
Fil: Rearte, Tomás Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Recursos Naturales y Ambiente. Cátedra de Química Analítica; Argentina
Fil: López Figueroa, Félix. Universidad de Malaga. Facultad de Ciencias. Departamento de Ecología y Geología; España
description In order to estimate microalgal carbon assimilation or production of Chlorella fusca cultures based on electron transport rate (ETR) as in vivo chlorophyll a fluorescence, it is necessary to determine the photosynthetic yield and the absorbed quanta by measuring the incident irradiance and the fraction of absorbed light, i.e., absorptance or absorption coefficient in the photosynthetic active radiation (PAR) region of the spectra. Due to difficulties associated with the determination of light absorption, ETR is commonly expressed as relative units (rETR) although this is not a good estimator of the photosynthetic production since photobiological responses depend on the absorbed light. The quantitative filter technique (QFT) is commonly used to measure the absorbed quanta of cells retained on a filter (AbQf) as estimator of the absorbed quanta of cell suspensions (AbQs) determined by using integrating spheres. In this study, light attenuation of thin-layer cell suspensions is determined by using a measuring system designed to reduce the scattering. The light attenuation is related to the absorptance as the fraction of absorbed light by both indoor and outdoor C. fusca cultures of different cell densities. A linear relation between AbQf and AbQs (R2 = 0.9902, p < 0.01) was observed, AbQf = 1.98 × AbQs, being 1.98 an amplification factor to convert AbQs values into AbQf ones. On the other hand, depending on the culture system, the convenience of the use of the absorptance, light absorption or specific light absorption coefficient expressed per area (thin-layer cascade or flat panel cultivators), volume (cylindrical and tubular photobioreactors), or chlorophyll units (any type of cultivation system) is discussed. The procedure for the measurement of light absorption presented in this study for C. fusca could be applied in other phytoplankton groups. The absorbed quanta as determined in this study can be used to express absolute ETR instead of relative ETR, since the first one provides much more relevant photobiological information of microalgae culture systems.
publishDate 2016
dc.date.none.fl_str_mv 2016-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/69336
Jerez, Celia G.; García, Carolina Belén; Rearte, Tomás Agustín; López Figueroa, Félix; Relation between light absorption measured by the quantitative filter technique and attenuation of Chlorella fusca cultures of different cell densities: application to estimate the absolute electron transport rate (ETR); Springer; Journal of Applied Phycology; 28; 3; 6-2016; 1635-1648
0921-8971
CONICET Digital
CONICET
url http://hdl.handle.net/11336/69336
identifier_str_mv Jerez, Celia G.; García, Carolina Belén; Rearte, Tomás Agustín; López Figueroa, Félix; Relation between light absorption measured by the quantitative filter technique and attenuation of Chlorella fusca cultures of different cell densities: application to estimate the absolute electron transport rate (ETR); Springer; Journal of Applied Phycology; 28; 3; 6-2016; 1635-1648
0921-8971
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s10811-015-0685-3
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s10811-015-0685-3
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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